Diese Präsentation wurde erfolgreich gemeldet.
Wir verwenden Ihre LinkedIn Profilangaben und Informationen zu Ihren Aktivitäten, um Anzeigen zu personalisieren und Ihnen relevantere Inhalte anzuzeigen. Sie können Ihre Anzeigeneinstellungen jederzeit ändern.
New insights into
exercise-induced
Mitochondrial Adaptations
Mitochondria
Cristae
Outer
Membrane
Matrix
Inner
Membrane
Lowell BB, and Shulman GI. Science 307: 384-387, 2005.
Defects ...
Exercise-induced changes in mitochondrial
content and mitochondrial respiration
Does antioxidant supplementation promote
o...
DefinitionsISEAL
Miller, K.L. (2012). Am J Physiol . 302: E496-499.
• Exercise: one-bout of exercise
• Training: a series ...
Mitochondrial adaptationsISEAL
Granata et al. 2016. FASEB J. 30(2): 959-970
TerminologyISEAL
Bishop, D.J., Skinner, T. (2014). Lactate Threshold.
In: “ESSA student manual for Health, Exercise and Sp...
0
2
4
6
8
10
12
14
16
18
20
[lactate]
Treadmill Speed (km/h)
8.5 10 11.5 13 14.5 16 17.5 19 20.5
L1
L2
L3
L4
L5
(HIT)
LI
L...
0
5
10
15
20
25
50 55 60 65 70 80 90 100
% VO2max
[lactate]
High LT
Low LT
L3
L3L2
L5
TerminologyISEAL
Bishop, D.J., Skinn...
TR TR UTUT
TerminologyISEAL
Baldwin et al. (2000). MSSE. 32:1648–1654.
Mitochondrial changes – cross-sectionalISEAL
Bishop et al. (2014). BBA. 140(4): 1266-1275.
Changes in CS (rats) – trainingISEAL
R² = 0.0063
0
10
20
30
40
50
60
70
80
90
100
0 10 20 30 40 50 60
CitrateSynthaseChang...
Changes in CS – trainingISEAL
Training intensity Training volume
R² = 0.797
0
10
20
30
40
50
60
70
80
0 50000 100000 15000...
Changes in CSISEAL
Granata et al. 2016. FASEB J. 30(2): 959-970
Changes in CSISEAL
Training intensity
Granata et al. 2016. FASEB J. 30(2): 959-970
Changes in CSISEAL
Training volume
* Significantly different (P < 0.05) from NT, † from RT
Granata et al. (2016). FASEB J....
Changes in CSISEAL
* Significantly different (P < 0.05) from pre-NT, # from post-NT
Training volume
Granata et al. (2016)....
Changes in complexesISEAL
Granata et al. (2016). FASEB J. In Press
Changes in CS (humans) – trainingISEAL
Training intensity Training volume
R² = 0.797
0
10
20
30
40
50
60
70
80
0 50000 100...
Changes in MR (mice) – trainingISEAL
Training intensity Training volume
R² = 0.7421
0
5
10
15
20
25
30
35
40
45
0 10 20 30...
ISEAL
Granata et al. (2015). FASEB J. in press
Changes in mitochondrial respiration
ISEAL Intensity & Mito Respiration
Daussin, et al. (2008). Am J Physiol. 295: R264-272.
↑36%
↑13%
ISEAL Changes in mitochondrial respiration
Granata et al. (2015). In Review
p160
RIP
140
v
PGC-1α mRNACREBMEF2 PGC-1α
AMPATP
AMPK
P AMPK
P
PGC-1α
MEF2
Mito
Genes
Nucleus
PGC-1α
PGC-1α
p160
ROS p38 M...
ISEAL
Granata et al. (2016) unpublished data
PGC-1α & p53 mRNA
ISEAL
Edgett et al. (2013) PLOS ONE. 8(8): e71623
PGC-1α mRNA
ISEAL
Edge et al. (2015) PLOS ONE. 10(12):e0141317
10 x 2 min
intervals
(90% VO2max, :
1 min rest).
Percival et al. (2015)...
Nuclear PGC-1αISEAL
Granata et al. (2016). Unpublished
Effect of training statusISEAL
Granata et al. (2016). Unpublished
ISEAL
p53 M
M
MDM2
PHF20
p53 M
M
nucleus
cytosol
stress
degradationstability
MDM2 MDM2
PHF20actiononp53
Cui et al. (2012)....
ISEAL P53 and PHF20
Cui et al. (2012). Nature Struct & Molec Biol 19, 916-924.
ISEAL
p53
P
nucleus
cytosol
stability
AMPK
P
p38
P
P53 and PHF20
Granata et al. (2016). Unpublished
ConclusionsISEAL
• Exercise training volume key for
mitochondrial content (citrate synthase)
• Exercise and training inten...
ISEAL Thanks
@BlueSpotScience
David.Bishop@vu.edu.au
Is there an optimal exercise stimulus for mitochondrial adaptations?
Nächste SlideShare
Wird geladen in …5
×

Is there an optimal exercise stimulus for mitochondrial adaptations?

7.102 Aufrufe

Veröffentlicht am

My talk from our symposium "New insights into exercise-induced Mitochondrial Adaptations" at the acsm meeting in Boston, 2016

Veröffentlicht in: Wissenschaft
  • Als Erste(r) kommentieren

Is there an optimal exercise stimulus for mitochondrial adaptations?

  1. 1. New insights into exercise-induced Mitochondrial Adaptations
  2. 2. Mitochondria Cristae Outer Membrane Matrix Inner Membrane Lowell BB, and Shulman GI. Science 307: 384-387, 2005. Defects  o Parkinson’s o Alzheimers’s o Huntington’s o Artherosclerosis o Cardiomyopathies o Diabetes ADP + O2  ATP
  3. 3. Exercise-induced changes in mitochondrial content and mitochondrial respiration Does antioxidant supplementation promote or impede skeletal muscle mitochondrial biogenesis? Autophagy and exercise-induced mitochondrial biogenesis Overview
  4. 4. DefinitionsISEAL Miller, K.L. (2012). Am J Physiol . 302: E496-499. • Exercise: one-bout of exercise • Training: a series of exercise bouts • Mitochondrial biogenesis: • the making of new mitochondrial components • it has been suggested that only measurements of the synthesis rates of mitochondrial proteins are indicative of mitochondrial biogenesis
  5. 5. Mitochondrial adaptationsISEAL Granata et al. 2016. FASEB J. 30(2): 959-970
  6. 6. TerminologyISEAL Bishop, D.J., Skinner, T. (2014). Lactate Threshold. In: “ESSA student manual for Health, Exercise and Sport Assessment”. 0 2 4 6 8 10 12 14 16 18 20 8.5 10 11.5 13 14.5 16 17.5 19 20.5 Treadmill Speed (km/h) [lactate] Lactate inflection Lactate threshold
  7. 7. 0 2 4 6 8 10 12 14 16 18 20 [lactate] Treadmill Speed (km/h) 8.5 10 11.5 13 14.5 16 17.5 19 20.5 L1 L2 L3 L4 L5 (HIT) LI LT L6 (SIT) Wpeak Terminology - LTISEAL Bishop, D.J., Skinner, T. (2014). Lactate Threshold. In: “ESSA student manual for Health, Exercise and Sport Assessment”.
  8. 8. 0 5 10 15 20 25 50 55 60 65 70 80 90 100 % VO2max [lactate] High LT Low LT L3 L3L2 L5 TerminologyISEAL Bishop, D.J., Skinner, T. (2014). Lactate Threshold. In: “ESSA student manual for Health, Exercise and Sport Assessment”.
  9. 9. TR TR UTUT TerminologyISEAL Baldwin et al. (2000). MSSE. 32:1648–1654.
  10. 10. Mitochondrial changes – cross-sectionalISEAL Bishop et al. (2014). BBA. 140(4): 1266-1275.
  11. 11. Changes in CS (rats) – trainingISEAL R² = 0.0063 0 10 20 30 40 50 60 70 80 90 100 0 10 20 30 40 50 60 CitrateSynthaseChange(%) Training Intensity (m/min) R² = 0.8753 0 10 20 30 40 50 60 70 80 90 100 0 5000 10000 15000 20000 25000 CitrateSynthaseChange(%) Training Volume (m/wk) Bishop et al. (2014). BBA. 140(4): 1266-1275.
  12. 12. Changes in CS – trainingISEAL Training intensity Training volume R² = 0.797 0 10 20 30 40 50 60 70 80 0 50000 100000 150000 200000 250000 300000 ChangeincitrateSynthaseactivity(%) Training Volume R² = 0.1097 0 10 20 30 40 50 60 70 80 0 50 100 150 200 250 300 Changeincitratesynthaseactivity(%) Training Intensity (%VO2max) Bishop et al. (2014). BBA. 140(4): 1266-1275.
  13. 13. Changes in CSISEAL Granata et al. 2016. FASEB J. 30(2): 959-970
  14. 14. Changes in CSISEAL Training intensity Granata et al. 2016. FASEB J. 30(2): 959-970
  15. 15. Changes in CSISEAL Training volume * Significantly different (P < 0.05) from NT, † from RT Granata et al. (2016). FASEB J. In Press
  16. 16. Changes in CSISEAL * Significantly different (P < 0.05) from pre-NT, # from post-NT Training volume Granata et al. (2016). FASEB J. In Press
  17. 17. Changes in complexesISEAL Granata et al. (2016). FASEB J. In Press
  18. 18. Changes in CS (humans) – trainingISEAL Training intensity Training volume R² = 0.797 0 10 20 30 40 50 60 70 80 0 50000 100000 150000 200000 250000 300000 ChangeincitrateSynthaseactivity(%) Training Volume R² = 0.1097 0 10 20 30 40 50 60 70 80 0 50 100 150 200 250 300 Changeincitratesynthaseactivity(%) Training Intensity (%VO2max) Bishop et al. (2014). BBA. 140(4): 1266-1275.
  19. 19. Changes in MR (mice) – trainingISEAL Training intensity Training volume R² = 0.7421 0 5 10 15 20 25 30 35 40 45 0 10 20 30 40 50 VmaxChange(%) Training Intensity (m/min) R² = 0.1364 0 5 10 15 20 25 30 35 40 45 0 2000 4000 6000 8000 10000 12000 14000 VmaxChange(%) Training Volume (m/wk) Bishop et al. (2014). BBA. 140(4): 1266-1275.
  20. 20. ISEAL Granata et al. (2015). FASEB J. in press Changes in mitochondrial respiration
  21. 21. ISEAL Intensity & Mito Respiration Daussin, et al. (2008). Am J Physiol. 295: R264-272. ↑36% ↑13%
  22. 22. ISEAL Changes in mitochondrial respiration Granata et al. (2015). In Review
  23. 23. p160 RIP 140 v PGC-1α mRNACREBMEF2 PGC-1α AMPATP AMPK P AMPK P PGC-1α MEF2 Mito Genes Nucleus PGC-1α PGC-1α p160 ROS p38 MAPK N R Fp38 P P PThr Thr HDAC P PSe Se NFAT HDAC RIP140 p160 P CaMK P Calcineurin NFAT P T tubule Muscle fibre Action potential Axon terminalAch Motor end plate Na+ K+ Ca2+ P p53
  24. 24. ISEAL Granata et al. (2016) unpublished data PGC-1α & p53 mRNA
  25. 25. ISEAL Edgett et al. (2013) PLOS ONE. 8(8): e71623 PGC-1α mRNA
  26. 26. ISEAL Edge et al. (2015) PLOS ONE. 10(12):e0141317 10 x 2 min intervals (90% VO2max, : 1 min rest). Percival et al. (2015) JAP. 119(11): 1303-1312 PGC-1α mRNA
  27. 27. Nuclear PGC-1αISEAL Granata et al. (2016). Unpublished
  28. 28. Effect of training statusISEAL Granata et al. (2016). Unpublished
  29. 29. ISEAL p53 M M MDM2 PHF20 p53 M M nucleus cytosol stress degradationstability MDM2 MDM2 PHF20actiononp53 Cui et al. (2012). Nature Struct & Molec Biol 19, 916-924. P53 and PHF20
  30. 30. ISEAL P53 and PHF20 Cui et al. (2012). Nature Struct & Molec Biol 19, 916-924.
  31. 31. ISEAL p53 P nucleus cytosol stability AMPK P p38 P P53 and PHF20 Granata et al. (2016). Unpublished
  32. 32. ConclusionsISEAL • Exercise training volume key for mitochondrial content (citrate synthase) • Exercise and training intensity key for mitochondrial respiration) • May be related to exercise-dependent effects on nuclear PGC-1α and p53
  33. 33. ISEAL Thanks @BlueSpotScience David.Bishop@vu.edu.au

×