3. Mdm2
Negative regulator of p53 tumor suppressor
gene
Discovery from transformed mouse cell line
Mdm2 over expression with Ras oncogene led
to tumor formation in nude mice
Human homolog is sometimes called Hdm2
9. E3 ligase activity
Targets both itself and p53
for degradation by
proteasome
Inhibitor of MDM2-p53
interactions is nutlin
MDM2 also interacts with
ubiquitin protease, USP7
that reverses
ubiquitylation to prevent
degradation with
proteasome
Fine regulatory circuit
18. References
Harris. Curtis C. et. al. Proceedings of the National Academy of Sciences.
http://www.pnas.org/content/103/6/1659/F1.expansion.html. Accessed May
7th
2013.
Proteasome. Wikipedia. http://en.wikipedia.org/wiki/Proteasome. Accessed
May 7th
2013
Mdm2. Wikipedia. http://en.wikipedia.org/wiki/Mdm2. Accessed May 7th
2013.
Knappskog et. al. MDM2 promoter SNP285 and SNP309; phylogeny and
impact on cancer risk. PubMed Central.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3260817/. Accessed May 13th
2013
Jayaraman A et. al. The interaction of p53 and MDM2 genes in cancers, in
silico studies and phylogenetic analysis. Biology and Medicine Vol 3 (3): 01-
12, 2011.
Databases/Tools Used: NCBI Protein. NCBI BLASTp. ClustalW2.
ClustalW2 Phylogeny. Cn3D. HomoloGene.