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Dendrobium Family – Orchidaceae Exhibits a vast diversity in vegetative and floral characteristics 1,600 Dendrobium species are recognized worldwide High value of crop – for flower and medicinal purpose D. husohanense- Anti-tumor and Anti- inflammatry D. longicornu - used to treat fever and coughs Micropropagation Seeds are minute and lack endosperm Micropropagation has been achieved using Shoot tip culture Seed culture (Immature and mature embryo) Auxiliary Bud culture Pseudobulb segment culture Shoot Tip culture Sterlization of Explant Shoot tips(0.5–0.8 cm) harvested from mother plants carefully washed in distilled water surface decontaminated with 0.1% streptomycin (20 s) 70% (v/v) ethanol (50 s) and 0.1% (w/v) HgCl2 (2 min) Thoroughly rinsed with sterilized distilled water Media Subculturing Micropropagation of Dendrobium From Pseudobulb segment Regeneration from Pseudobulb Segment Cultures Pseudobulb segments of about 0.5-1.0 cm excised from the 1 year old in vitro raised seedlings Any leaves or roots, if present, were removed from the segments prior to inoculation Each segment had one or two axillary buds. Single pseudobulb segment was cultured in test tubes (25 mm × 150 mm), each containing 12 mL half-strength MS basal medium supplemented with BAP 1.0 mg L-1 individually or in combination with NAA at 1 mg L-1 The medium was solidified with 4 g L-1 agar Rooting of Regenerated Shoots (Pseudobulbs) Small clumps of shoots having 2-3 pseudobulbs (3-4 cm in length) were cultured in test tubes (25 mm × 150 mm) Each containing 12 mL half-strength MS basal medium supplemented with or without 1.0 mg L-1 IAA or IBA or NAA. The cultures were incubated for 3 months under the conditions as described above. The pH was adjusted to 5.8 before autoclaving at 121°C, 15 lb in-2 for 15 min. Micropropagation of Dendrobium From Auxiliary bud Stem (1–2 cm long), each comprising a node and axillary bud are used as explant- wash in running tape water for 15-20 minute Surface sterilization with- - 10 % (v/v) NaClO solution for 10 minute - 0.1 % (w/v) HgCl2 for 2 min - washing 5–6 times with sterilized distilled water The explants were shortened to 3–4 mm after the removal of leaves, dry sheaths and other external tissues Micropropagation of Dendrobium from immature seeds Capsules collected from hand-pollinated plants after 8–14 wk of pollination Surface-disinfected in 70% ethanol for 30 s, followed by 1.0% sodium hypochlorite with two drops of Tween 20 per 100 ml for 10 min and rinsed five times with sterile distilled water After sterilization, the c

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