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STATION MARDI CAMERON HIGHLANDS PETI SURAT 19,
PEJABAT POS TANAH RATA, 39007 CEMERON HIGHLANDS,
                           PAHANG.




                   Industrial Training Report

       Submitted in Partial Fulfillment of the Requirement

           for the Award of D.Sc (hons), Planting, UiTM

                    Faculty Applied Science

                  University Technology Mara




                               By




                 MARZUQI BIN MOHD SALLEH

                            OCT 2009



                                1
Student’s Declaration




I declare that this report is the result of my own works except as cited in the
references. The report has not been accepted for any diploma and is not
concurrently submitted in candidature of any other diploma.




Signature     :




Name          : Marzuqi Bin Mohd Salleh




Matrix No     :




Date          : 28 January 2010




                                       2
ACKNOWLEDGEMENTS

        Writing reports has become a practical way unusual for me; an
experience not be alone with the blessings of Allah Al-mighty. I have several
people to thank for helping me achieve this exceptional performance and
extraordinary.




        First, I have a family, especially my father Mrs Mohd Salleh Bin Ahmad to
thank for providing emotional support and inspiration. He continued to encourage
me to stay motivated and focused. Secondly, I have a mother Salbiah Binte
Mahamud and my family to thank for their extraordinary patience, moral and
financial support.




        In addition, I was really doing my practical training for 5 weeks. I have
been conducting, Mrs. Zainal Bin Salleh, all friends and all stations Mardi support
have helped me a lot to thank you for doing good deeds to keep running on
practical training.




        Last but not least, I would also like to thank all lecturer practical training,
all my friends, other students for their continuous support in the training practical.




        In short, for every person who has touched my life, and especially those
involved in writing reports misery, I thank you. Thank you from my heart policy,
because I can’t do this without you. Thank you very much, you are the best!




        Thank you.




                                          3
OBJECTIVES

•   To educate the DPIM students to become a professional person with the
    characteristics are credibility, responsible hardworking level. And also are
    sincerely religious in exploring the earth with responsibility especially in
    agriculture.


•   For build the attitude to DPIM students. So that, the DPIM students will have
    are courage, hardworking and also strong in physically and mentally as well
    as. Knowledge among DPIM students need to establish.


•   To develop estate with plenty types of crops as convenience when the
    student having their practical training.


•   To develop the landscape / garden that is beautiful and interesting places in
    the campus not just as a recreation park but also as an education and
    exposure to the students about method and landscape design that is very
    interesting.


•   To upgrade the product produce by this farm by using new method in
    agriculture as well as a source of income for UiTM Perlis.


•   Complete the equipment that is use for agriculture for the student convenient
    to achieve goals and to rise up the student potential in agriculture.


•   To help general academic department and student affairs in any types of
    facilities that farm department should have.


•   To have are responsible, positive attitude and with the highly moral ethics of
    workers when giving their services.




                                          4
CONTENTS

      Content                                            Page
________________________________________________________________
Student’s Declaration                                      i
Acknowledgements                                           ii
Objectives                                                 iii
Content                                                  iv – v


CHAPTER 1 : COMPANY PROFILE                              1-2


CHAPTER 2 : EXECUTIVE SUMMARY
2.1 Programs and Activities                               3-5


CHAPTER 3: PERSONAL INVOLVES
3.1 Who are involves                                      6-8
3.1.1 Supervisors from MARDI


CHAPTER 4: EQUIPMENT OR WORK PROCEDURES INVOLVES
4.1   Nature of crops                                      9
4.1.1 The Chrysanthemum (Kekwa plants)                   9 - 10

4.1.2 Tea planting                                        11

4.1.3 Apple (Malus Domestica)                             12
4.1.4 Nursery                                             13




4.2   Field Maintenances                                  14


4.2.1 Control of Grass                                   14 – 16

4.2.2 Pruning                                             17

4.2.3 Mulching                                            18




                                   5
4.3    Information

4.3.1 Setting the drip system            19

4.3.2 Technology of Fertigation          20

4.3.3 Soil and water conservation        21

4.3.4 Asexual Propagation                22

4.3.5 Landscaping                       23 - 25

4.3.6 Compost                           26 - 27

4.3.7 Propagation techniques            27 - 30

4.3.8 Outdoor activities                 31




CHAPTER 5: CONCLUSION
32


CHAPTER 6: REFERENCES                    33


CHAPTER 7: APPENDIX                     34 – 40


Appendix 1                              34 – 35


Appendix 2                              36 – 39


Appendix 3                               40




                                    6
CHAPTER 1: COMPANY PROFILE

Welcome to Agro Technology Park in MARDI Cameron Highlands
The Cameron Highlands countryside is endowed with inexhaustible historical,
natural and human riches, generously revealed to the visitor. Its traditional
villages, enhance and complement a wonderful natural environment, with their
local character, traditional architecture and village planning.

The district of Cameron Highlands countryside is endowed with inexhaustible
historical, natural and human riches, generously revealed to the visitor. Its
traditional villages, enhance and complement a wonderful natural environment,
with their local character, traditional architecture and village planning.


The MARDI Cameron Highland’s Stations was first established by the British in
1925. It was then known as the Federal Experimental Station. Tea was first
introduced in the country in 1925 and these early tea bushes are growing healthy
at the station until today. The first tea factory built in 1935 is still capable of
processing black tea.

The Agro technology Park MARDI Cameron Highlands is located within the
station and was officiated by His Royal Highness DYMM The Sultan Of Pahang
on 14th of June, 2003. Since then, about 65,000 visitors, visit the park each year.


The Agro technology Park is only 1km away from the town of Tanah Rata and
easily reaches by car, taxi, bus or even by foot. Among the major attractions are
the English Garden, strawberry production, vegetable and cut flower production,
green house research and the sale centre.




                                          7
Figure 1.0 Office.




Map of Agro Technology Part




          Figure 1.2 all areas in the MARDI Cameron Highlands.




                                      8
CHAPTER 2: EXECUTIVE SUMMARY

2.1 Programs and Activities


We are 3 members. My group’s members are:
   1. Marzuqi Bin Mohd Salleh                     2008502409
   2. Mohamad Syazwan Bin M. Rosley               2008766593
   3. Muhamad Hafiz Bin Shamsudin                 2008754211


    Date               Unit         Conductor                     Activity

18 to 23      Soil and water    Pn. Aini Hayati       1. Transplant.
Nov.2009      conservation.
                                                     •   The chrysanthemum
                                                         plant.

                                                      2. Weeding.


                                                     •   At the soil and
                                                         conservation plot.
24 to 25      Cutting, Roses,   En.Yazid              1. Weeding.
Nov.2009      Orchid            Mahiyuddin
              Management.                            •   At the Gerbera plant.


                                                      2. Transplant.


                                                     •   The chrysanthemum
                                                         plant.

                                                      3. To make a simple
                                                         landscape.


                                                     • At the orchid house.
30 Nov. to    Commercial        En. Mustafa          1. Weeding.
04 Dis.2009   Project.          Kamal
                                                     •   At the arachinis




                                    9
hypogaea L.

                                                 2. Soil reparation.
                                                 3. To fill up the
                                                     cocopeat into the
                                                     poly bag.
                                                 4. Medium mixture.
                                                 5. Setting up the drip
                                                     system.
                                                 6. Compound clearing.


                                                 •   At the arachinis
                                                     hypogaea L.


                                                 7. Pruning.


                                                 •   At the arachinis
                                                    hypogaea L.
07 to 09 Dis.   Sub-tropical     En. Osman sah   1. Planting the orange
2009            project.                             plant at the orange
                                                     plot.
                                                 2. To make the wedge
                                                     grafting.
                                                 3. Transfer the orange
                                                     plant at the nursery.
                                                 4. To make the cut
                                                     grafting.
                                                 5. To make the
                                                     marcotting.




10 to 14        Tea plantation   En. Muhammad    1. Clearing the morning
Dis.2009        and banana       Nor                 glory plant at the tea
                khaki.                               plot.
                                                 2. Weeding at tea area.




                                       10
3. Visit to Nursery.
15 to 17   Agronomy     En.Mohammad        1. Pruning tomatoes
Dis.2009                Abid                   plant.
                                           2. To fill up the
                                               cocopeat into the
                                               poly bag.
                                           3. Planting the pearl
                                               grass at the edible
                                               garden area.




21 to 22   Entomology   En. Zulkifli bin   1. Weeding.
Dis.2009                Mohd Saaid
                                           •   At the edible garden
                                               and clearing that
                                               area.

                                           2. Planting.


                                           •   Planted the vegetable
                                               plant into the pots.

                                           3. Pruning.


                                           •   Tomato plants at the
                                               tomatoes plot.




                            11
CHAPTER 3: PERSONAL


3.1 Who are involves


  3.1.1 Supervisors from MARDI


  1. Name              : Puan Aini Hayati binti Abd. Rahman


      Position         : Assistant Research Officer (Q32)


      Unit             : Soil and Water Conservation


      Status           : Married


     Name of Husband : Jamaludin bin Ismail




  2. Name              : En. Jamaludin bin Ismail


     Position          : Research Assistant (Q17)


     Unit              : Soil and water Conservation


     Status            : Married


     Name of Wife      : Puan Aini Hayati binti Abd. Rahman




  3. Name              : En. Yazed bin Mahiyuddin


     Position          : Research Assisstant (Q17)


     Unit              : Cutting, Roses, and Orchid Management


     Status            : Married


                                    12
4. Name      : En. Mustafa Kamal bin Hanafiah


  Position   : Research Assistant (Q17)


  Unit       : Commercial Project


  Status     : Married




5. Name      : En. Osman Sah bin Md Daud


  Position   : Research Assistant (Q22)


  Unit       : Sub. Tropical Project


  Status      : Married




6. Name       : En. Muhammad Noor bin Bahauddin


  Position    : Research Assistant (Q23)


  Unit        : Tea Plantation and Banana Khaki


  Status      : Married




                          13
7. Name       : En. Mohammad Abid bin Ahmad


  Position    : Research Officer (Q41)


  Unit        : Agronomy


  Status      : Married




8. Name       : En. Saiful Zaimi bin Jamil


  Position    : Research Officer (Q41)


  Unit        : Entomology


  Status      : Married




9. Name      : En. Zulkifli bin Mohd Saaid


  Position   : Assistant Research


  Unit       : Agronomy


  Status     : Married




                           14
CHAPTER 4: EQUIPMENT OR WORK PROCEDURES
                                    INVOLVES


4.1      Nature of Crops


There are various types of green plants, fruits and vegetables. In Cameran Highlands,
plants that live there in the cold temperatures. Therefore, MARDI cameran highlands
also provide opportunities for visitors who want to see the various plants that cold
weather.




4.1.1 The Chrysanthemum (Kekwa plants)

       They came from China. They are living ‘Short-day-plant’. They need more
extra light at night (>10.5 hours) for a shape of flower. Get the suitable length of
the chrysanthemum stem about (100 cm) in time of period and control for slowing
the flower to grown up, They need more extra light of 100 watt ± 4 hours at night
during in 5 to 8 weeks. Used lamp until the plants grown about 35 cm high for 5
to 8 weeks. A shaped of flower can be seen after 2 to 3 weeks and they
harvested in 6 to 10 weeks after switch off the lamp. In one season is about 3 to
4 months. Spray/pompon species were planted >90% in Cameron Highlands
than standard species. They are also planting with propagation by cutting and
seeds. Popular species are Reagon Sunny, Reagon Purple, Jaguar Purple, Red,
Monalisa Yellow, Monalisa White, Dublin Red and Spider White.

The technology light with cutting propagation method of Chrysanthemum plants:

 The Chrysanthemum plant need more extra night light (Short-day-plant).
 After planted the Chrysanthemum tree it needs more extra light of 100 watt ±
      4 hours at night. Also, they always need switch on the light during in 5 to 8
      weeks after that switch off the lamp in 6 to 10 weeks.
 Used extra light every night for :

         -To get the suitable length of Chrysanthemum stem about (100 cm) in
         period date.



                                          15
-To control for slowing to grown up

In plant planting, the transplant is very important because to give plant growth on
the soil actual and get direct nutrient and absorb water or air from soil.

Normally, in this case they used propagation by cutting. As we know, propagation
have more advantages such as to retain the desirable characteristic of the
mother plant and increase or multiply the number of plants rapidly.




     Figure 4.0 Chrysanthemum shows that the way of retransplanting.




     Figure 4.1 show the place where chrysanthemum tree are planted.



To produce the best quality of chrysanthemum plant they need more cool
weather and produce the colourful flower.



                                         16
4.1.2 Tea planting


       Tea planting is a living green leaf in family of Camellia and they came
from China, Tibet and North India. There are two varieties such as small leaf and
wide leaf. In small leaf variety, known as Camellia sinensis, live well at district of
highland in Cameron Highlands. In wide leaf variety, known as Camellia
assamica, most live well at district of tropical. In tea planting, there are colorful
dark green, small size and white flower.

   Figure 4.2 show that the current working of the plant weeded control.




                                           17
Figure 4.3 show that way of planting in the Cameron Highlands.
4.1.3 Apple (Malus Domestica)


         The apple thee is temperate plants. Dormant time for winter (<7°C, until >
100 hours) need to promote the flowers. In tropical compound, there are pruning,
bend the branch, drop leaves, and hormone spraying (Dormex 3%). Highlands
area >800m have potential to planting apple from varieties that need short
dormant time (~300 hours) like Anna, Grany Smith and Rome Beauty. After >3
years planted, all leave will be dropping. New leaves and flowers will be growing
up after 2-4 weeks. Schedule of dropping the leaves are very importance to do so
that the production of flower occur in less rain season (open area). Fruit will
mature after 125 days flowering. Produce two time a year (May- June and Nov-
Dis.).




          Figure 4.4 show that the place where apple tree are planted.




         Figure 4.5 show that way of planting in the Cameron Highlands.


                                         18
4.1.4 Nursery

4.1.4.1 Planting of tea plant.


        Learn the techniques how to make planting the tea plant in nursery?

•   Select the suitable stem and free pest or disease.
•   Length of stem about 5 to 6 cm.
•   Put the hormone (IAA) at the end of stem.
•   To planting into the poly bag.
•   Follow by watering.
•   All poly bags must be put into the white plastic. Then, close be used to white
    plastic because they are enough nutrients, enough humidity, and weed
    controls.
•   For our information, in that case for tea planting they used to plant by cutting
    propagation because they are easy grown rapidly.
•   After 1 year they will transfer to the field.




           Figure 4.6 show that the place where tea tree are planted.




                                            19
4.2       Field Maintenances


      •   Each plant must have care well to plant a garden so fertile. Care was often each
          week.




4.2.1 Control of Grass

1. Manual Control


              -Used the mulching (e.g. plastic, cover crop and etc).

              -Used the labor energy.

2. Mechanical Control

              -Used the tractor

3. Biology Control

             -Used the insect such as bacteria or virus.

4. Chemical Control

             -Used the chemical spray.

             -Save cost.




                                            20
figure 4.7 show that weeding method with good.




Grasses Problem

   Grass is no need in plant. It makes trouble to plant.
   It challenged between the plant to get nutrient, water, space and light.
   Some grasses needs in plants such as Maman Pasir.
   The Maman Pasir is insect control.
   Cost of grass is 10 % to 35 % than cost of planting.




Disadvantages of grass

•   A challenged with plant. The plant become dwarf and loses profit, it caused
    the grass more quite grown than plant.
•   Drain irrigation will become trouble.




General Recommendations for Weed Management




                                            21
 Likewise with insects, identifying all the different weed types in the farm and
   understanding their means to survival and their role in agro-ecosystem are
   important steps towards their management.
 It is also essential to adhere to this basic principle in managing weeds. Which
   is minimizes to competition between the weed and the cultivated crop.
 For weed control, the following methods and materials could be used.




        figure 4.8 show the weeds that have been extracted by hand.




1) Cultural Practices

-The use of cultural practices, which limit weed development include manual
weeding around the base of the plant and on the beds, crop rotation, forking,
digging and burying, rot ovation, close spacing and the use of ground covers.




2) Mulching

-The use of dried grass mulch on the beds and regulated use of plastic mulch to
cover the surface of the beds before planting help to reduce weed infestation.




                                       22
3) Physical Control

    -In some instances, flame weeding could be used.

    •   Weeds are major problem in any farm. They need to be managed well even
        at an early stage of cultivation; otherwise the following problems will result in:

a. High costs for labor and time taken for management (almost 50%) to remove
    these weeds;
b. Adverse effects on crops when they start to complete for water, nutrients and
    light. They can stunt crop growth and cause loss of yield.
c. Harboring of pest and pathogens, such as Cleome rutidospema, which is host of
    flea beeties, H. undalis and P. xylostella.




•   To avoid the above problems, early interventions are necessary to prevent the
    build-up of high weed populations, especially during rainy seasons. The major
    weed species found in a vegetable farm include both broadleaf and grasses. The
    major grassy weeds include nutgrass, Eleusine indica, Cyperus rotundus and
    Paspalum conjugatum. The broadleaf weeds commonly found are borreria
    latifolia, C. rutidospema, Asystasia gangetica and Amaranthus spinosus.
    Naturally, the type of weed species found in an area depends on the inherent soil
    conditions and agronomic practices typical of the farm.




    4.2.2 Pruning

    •   Pruning is the activity of removal of a plant to improve its appearance and
        health and to control it growth and shape.




    Purposes of the pruning are:

    •   Removed the dead / diseased portion /part.
    •   To get the shape of the plant-landscape.
    •   Sanitation.



                                              23
The different between pruning and thinning is:

•   Pruning is the removal of portion of the plant to improve its appearance and
    health and to control it growth and shape whereas pinching is the removal of
    the terminal shoot on each branch of the flower and allows the lateral shoot to
    develop, thereby creating fuller plant.




             figure 4.9 shows the results of clipping trees and a shady.




4.2.3 Mulching

•   Is the process of spreading generally organic plant materials between and
    around the plants to cover the soil. Mulches reduce soil moisture loss, reduce
    soil temperatures, control weed and increase organic matter content after
    decoying. Example of materials used: Leaves, rice straws, grass clippings,
    cocoa beans, sawdust, oil palm shell and ground fir bark.
•   A plastic Mulching used to site location plants as to control the weeds.


                                         24
•     White color: A minimizes attack from insect to perch under leaf.
•     Black color: For soil treatment.


                   -To kill the pathogen from soil.




             figure 4.10 show the mulching completed installed.




             figure 4.11 to show the installation is in progress.

4.3      Information

4.3.1 Setting the drip system

In all the plants, flow system is very important to planting because to support the
plant growth well. From planting on flower bed (planting Chrysanthemum) it used
the drip system. What we did it? Firstly, from the drip system has loose. So, we



                                           25
learn the technique How to taut the rope. Most importantly, we should have bend
crossing string well. It’s because to support the drip system flow stay in well
position and easy to manage.

•   Netting is used to support the Chrysanthemum plant. It needs to move up
    when the plant starts increasing to grown up.




           Figure 4.12 show how to made a setting the drip system




4.3.2 Technology of Fertigation

    Fertigation is a combination of two words ‘Fertilization’ and ‘irrigation’. The
concept of this system is to deliver fertilizer in form of a solution to the root zone
through the irrigation system. This technology can be the applied in the soil or
soilless, in the open field or under protective structive structure (PS), However,


                                         26
this system recommended implement in soilless media under prodective
structure to reduce the risk of soil borne disease and disease of leaves, shoots
and fruit on the plants. In addition, PS can also avoid the fertilizer solution given
to the plant to be too liquid. Therefore, Fertilization is not effective. Media used is
coconut coir. Dust or better know as cocopeat because it is cheap and easily can
obtained from local source.

   Equipment used in this system is stock tanks, solution tanks, filter, main pipe,
delivery pipe, micro tube and dripper. Electrical power pump may be used as an
alternative to the gravity method to deliver the fertilizer solution to the plant if
insufficient water pressure or to irrigate a vast area of the farm. The used of the
limer will make the fertigation system more efficient in terms of management of
the fertilizer solution to the plant. Application of fertigation of fertigation system
has many advantages, which may reduce the operation cost, improve quality and
productivity, time saving, reduce the number of labour and labor cost and
increase the efficiency of field management. Besides, fertilizer and water are
effectively used.

   Soilless media can reduce the risk of soil borne disease and avoid the soil
pollution caused by salt accumulation. Fertigation is a clean system, odorless,
environmental friendly and crop such as tomato, red chili, cucumber, zucchini,
eggplant, melon, capsicum and strawberry.




4.3.3 Soil and water conservation

   Soil erosion by water is main soil degradation process in CH. Erosion process
is detachment and transportation of soil particles by erosion agent (i.e water and
wind) and ended with deposition. Erosion carried together with nutrients, and
pesticides resulting in eutrophication (nutrients enrichment in rivers and lakes).
Objective of the soil and water conservation plot is to show the effectiveness of


                                          27
cover crop and horizontal arrangement of soil bunds. Visitors are exposed to the
techniques in measuring soil loss, surface runoff and rainfall. Rhizotron is
constructed to show the root growth of daylily which is comparable to vetiver
grass is recommended by FAO to control erosion on slopes.

There are six construct plots in soil and water conservation area:

1. Cabbage (Capitata)
2. Kum Chum (Hemeocalis Fulva)
3. Cover Crop (Desmodium sp.)
4. Vetiver grass (Vetiveria zizanoides (L) Nash )
5. Cabbage (Capitata)
6. Lycium Sinensis (Kau Kei)




               Figure 4.13 show that how to use the clinometer.

 Slope for soil and water conservation plot is 5°.
 Used the clinometer for determine or measuring the slope.


In researched, the Desmodium sp. is the best reducing in soil erosion.

4.3.4 Asexual Propagation


•   Is the reproduction of plant which is not involved the fusion of gametes. The
    propagation utilizes the vegetative parts.



                                         28
•   Part of plant can be used in asexual propagation.


 Leave
 Stem
 Root
 Embryos
 Pollen grains
 Shoot tip
 Callus




Purposes:


•   To retain the desirable characteristic of the mother plant.
•   To increase or multiply the number of plants rapidly.
•   To shorten the bearing age of plant, especially in fruit trees.
•   To prevent the plant species from being lost or extinct.
•   To produce superior strains and disease-resistance plant that will be best
    suitable under given climate and soil condition.




                                          29
Figure 4.12 show that how to made the wedge and marchoting
                                        methods.




4.3.5 Landscaping

•   Is the art of purposely changing the natural features that exist out-of-doors,
    with the intention of making the environment more attractive or functional.
    This is done to meet the extended needs for man for his physical needs,
    mental health and comfort.




Functions of plants in Landscape design. Contribute to landscaping in two major
functions:

a) Plants Physical Function:

-Control Erosion

-Soothes the climate

-Provide screening from light, wind and from unwanted views

-Create a form of direction

-Protect wild-life




b) Plants Esthetic Function:

-Create spatial effect

-Planning views

-To soften the concrete effect on the environment

-To connect concrete and structure with the ground effect

-Creating unity effect on various elements in particular area


                                        30
figure 4.13 show the landscape area.




Figure 4.14 show the road landscape.




                31
figure 4.15 show the cuctus garden area.
As we know, Plants may be propagated under two (2) general categories: Sexual
and vegetative or asexual propagation.

    Sexual Propagation

•   Is the common method of reproduction and multiply of plants. This is usually
    done with the use of seeds and spores. Garden vegetable like tomato,
    eggplant, radish, carrot, mustard and legume crops (Cawpea, Munyo and
    Peas) are generally propagated by seeds. Fruits trees such as avocado,
    santol, jackfruit, guava, mango, etc. are also propagated by the use of seeds,
    although in some cases, the resulting plant may not come true to type and to
    not have the same characteristic as the parent plants.
•   Seeds are still the most economical and fastest means in the reproduction of
    plants. However, some plants like fruit trees produced from seeds very in
    their characteristics. Hence, we cannot always expect springs or progenies.
    Ferns and mosses are by natural propagation by spores.




Asexual Propagation

•   Is the development of new plant natural or artificial with the use of seeds,
    since it does not involve the function of sex, it is commonly referred to as
    asexual.




                                         32
Disadvantages:

•      There is possibility of disease being transmitted from the parent to the new
       individual plant.
•      Some source of certified scion or buds sticks is a usual problem.




 4.3.6 Compost

What is Composting?

      Zucconi and Berthold (1987)defined composting as a controlled bioxidative
       process that:

i.      Involved a heterogeneous organic substrate in the solid state.
ii.     Evolves by passing through a hemophilic phase and a temporary release of
       phototoxic and ;
iii.     Leads to production of carbon dioxide, water minerals and a stabilized
       organic matter (compost).




Factors Affecting Composting Process

•      Micro-organism
•      Carbon : nitrogen ratio (C/N ratio)
•      Particles size



                                             33
•   Aeration
•   Moisture
•   Temperature
•   Size of heap




Cube Box Cocopeat

•   When wallowed into the water and they are also to be enlarge used to fill up
    into the poly bag are about 10 poly bags.




                      Figure 4.16 show the Cube Box Cocopeat.

Other Benefits of compost are:

•   It stores and slowly releases some nutrients, for some effective use to the
    plants.
•   The organic matter is increased, thereby the root structure, especially the root
    hairs of the plants are increased.
•   It balances the pH of the soil by acting as butter.
•   By recycling wastes into compost, the environment becomes more
    sustainable.
•   It attracts earthworms and soil organisms that are responsible for making the
    soil a ‘living’ soil.
•   It reduces soil erosion
•   It suppresses certain soil borne diseases
•   It reduces reliance on petroleum based fertilizers




                                         34
Manage Labour

•   Weed control
•   Organic fertilizer application
•   Compost making
•   Crop production measures
•   Post harvest handling and marketing




4.3.7 Propagation techniques

•   Are the studies of process of extending the life of a particular species of plant
    such as propagation by grafting like wedge grafting, and whip or tongue
    grafting.




        Whip or Tongue Grafting

•   This method has districts advantage of allowing a much greater area of
    cambial contact than either cleft or saddle grafting.




Procedure:

•   Start in the same manner as cleft or saddle grafting
•   After cutting back the rootstock, make a smooth slanting cut about 3-5 cm
    long where you intend to make the graft
•   About 1/3 from the cut surface, make a downward cut so it would form some
    sort of a tongue that point upward
•   Make similar but reverse cut on the scion
•   Fit them together making sure their tongues interlock snugly and bind with a
    plastic tape.




Method of Artificial propagation



                                         35
Cutting

Is a detached portion of the selected mother plants intended to be multiplied, of
which at least one-third to one-half of the length is inserted in the soil or rooting
media with the sole purpose of producing new plant. Cuttings may be divided into
tree classes:

   Root cuttings made from matured roots as in the case of rimas.
   Leaf cuttings as in Begonia
   Stem cuttings soft wood cuttings may either be made from young and
    immature parts of the plants as in the case of Gumammela, San Francissco
    and Papua. Hardwood cuttings are made from matured twigs of the plants
    intended to be produced as in the case of Bougainvillea. Lately, success has
    been made in producing rooted Chico and citrus with the use of cuttings
    dipped in root hormone solution.




Factors to be Considered in Grafting

          The weather conditions and the stage of plant should be considered for
the success in grafting. Grafting may be done at the beginning of the dry season
and rainy season. The scion should be well-prepared from selected pedigreed
trees, the characteristics of which are to be perpetuated. In the same manner, the
stock should be healthy, vigorous and free from diseases and insect pests.

Basic Requirements of Plant Life

1) Water
2) Energy
3) Air
4) Medium




Water

   Water enables all the major metabolic processes within the plant.
   Water acts as a solvent to convey nutrients, foods and other substances
    throughout the plant.


                                         36
   Water provides plant cells with their internal Turgor Pressure.
   The quantity and quality of water in important


       -Too much water in the soil can cause the plant to suffocate. Wet roots
       are more susceptible to rot or mold (kulat).

      -Too little water will result in loss of turgidity, wilting. Metabolic processes
       will be reduced, leading to growth retardation and possible death.

       -Water can be unsuitable for plants if it contains: Pollutants such as
       herbicides; high salinity: mud or silt.




Marcotting

•   Is the process of inducing Brach or twigs to produce roots while still attached
    to the parent plant.




Procedure:

•   Firstly, to prepare all equipment those like knife, suitable plant, medium,
    hormone and etc.
•   To select the suitable plant.
•   Make a girdle around the branch about 1 to 2 cm wide or depend on the size
    of the branch.
•   Scrape off the cambium layer completely to prevent the re-growth of the bark.
    It may help to apply a rooting compound to the exposed the stem.
•   Wrap the cut surface with about a handful of medium. If the medium is too
    wet, the stem may decay.
•   Wrap with a piece of plastic film about 15 × 20 cm around the ball of medium
    and tie all around with cotton twine. The plastic film keeps down loss of
    moisture from the medium.
•   As soon as you see enough roots throughout the plastic film, cut the
    marcotted branch from the mother plant. It may be from 2 to 12 months after
    marcotting depends on the crop.




                                          37
•   Cutting may be done in gradual basis until if attain sufficient roots to live by
    itself as indicated by a new flush of growth (a critical period for the new plant).




                        figure 4.17 marcotting completed.




4.3.8 Outdoor activities

We have an opportunity there with staff Mardi mountain climbing.




                                          38
CHAPTER 5 : CONCLUSION

         In conclusion, we have got more information and knowledge in MARDI
Cameron Highlands. One of all, many thank to MARDI Cameron Highlands
organizers because they were accepted us as practical training students there.
As long as 5 weeks we have completely done our practical training, also we have
7 units followed such as Ms. Aini Hayati bt Abd Rahaman in unit Soil and Water
Conservation. With her, we have learned about Chrysanthemum plant.

         Secondly, followed by Mr. Yazed bin Mahiyuddin in unit of Cutting,
Roses, Orchid Management. In his unit, we learned that how to make transplant
the Chrysanthemum plant and pruning at Gerbera plant. Thirdly, we followed En.



                                      39
Mustafa Kamal bin Hanafiah in unit of commercial project. With his, he teaches to
us how to make that filled up cocopeat into the poly bags, put mulching on the
bed and make medium mixture (cocopeat:Organic fertilizer)(1:1,5:1). We learned
about organic plants there are no need chemical matters and they use biological
control for maintenance. After that, we have followed Mr. Osman Sah in unit Sub
Tropical Project. With his, we learned about propagation techniques such as
marcotting and cutting. We have got experience to make marcotting and cutting.
It was interesting for us to learn.

             Then, we have followed Mr. Mohammad Nor in unit Tea Plantation. We
were learned to pruning and weeding. Morning Glory is challenging between tea
plant and Morning Glory, so they need to cut off. Propagation for tea is cut
grafting. Tea plant has live for many years. After that, we have followed Mr.
Mohammad Abid in unit Agronomy. We have put mulching on the bed, filled up
the cocopeat, pruning and harvest tomatoes. Lastly, we have followed Mr. Saiful
in unit Entemology. In his unit, we have learned about pest and disease for
example, there are four main insects in Cameron Highlands such as Plutella
Xylostella, Leaf Minor, Red Spider Mites and White Fly. For control component,
there is Integrated Pest Management (IPM) such as parasite, predator, bacteria
and virus.

        Finally, we have apologies to all organization. We hope all those
knowledge we can use for next time. All profits that come from God and all come
that from our lack. Virtually all gains and losses have come from Allah S.W.T.




                                        40
CHAPTER 6: REFERENCES

Derector, infomardi : Chrysanthemum, Info Center Argo Technology, MARDI,
        Cameron Highlans.

Derector, infomardi: tea, Info Center Argo Technology, MARDI, Cameron
        Highlans.

Derector, infomardi: fruits, Info Center Argo Technology, MARDI, Cameron
        Highlans.

En. Zulkifli bin Mohd Saaid, A. R. (2009), Agronomy, MARDI Cameron
        Highlands.Pahang.

Aro technology part, welcome to Cameron HighlandsSelamat, Pahang.

Puan Aini Hayati, A.R. (2009), Sistem Fertigasi, Bahagian Pengurusan dan
        Pemuliharaan Sumber Tanah, MARDI Cameron Highlands.

Prof Rasyid: Landscape AGR 112, propagation, UiTM Arau, Perlis.




                                      41
CHAPTER 7:APPENDIX

                                         Appendix 1

  Crop pests
  Major insect pest attack should be given particular attention to plants grown under the
  protective structure of plants. Among the main insect pest, the type of damage and
  control methods for plant tomatoes, chilli and melon are as follows.

  Plant                Pest                        Damage                          control

Chili          Afid (Aphis gossypii)         •    Tues suck fluid            •   Mulching plastic
                                                  leaves, young leaves           shine can prevent
                  •   Flea leaf green
                                                  wrinkled                       landing afid, reduce
                      kehijau parents,
                                                  berkerinting                   the number of afid,
                      there below the
                                                  produce klorosis               can further reduce
                      surface of the
                                                  spots, stunted fruit           disease virus
                      leaf
                                                  trees and attacked             infection.
                                                  virus disease                  • natural enemies
                                                                                 such as tortoise
                                                                                 beetles can help
                                                                                 reduce the number
                                                                                 of afid.
                                                                                 Selected chemical
                                                                                 poisons such as
                                                                                 profenofos and
                                                                                 imidacloprid
                                                                                 spraying when
                                                                                 necessary and
                                                                                 repeated 7 days
                                                                                 later.




                                             42
Chili   Kutu Trips (Thrips         •   Tues suck fluid leaves,           •   mulching plastic
        sp.)                           leaves become smaller                 shine prevent and
                                       and privation, wrinkled               reduce the number
            •   Insects fine,
                                       and curved tapering                   of early attacks.
                yellow, found in
                                       upward, brown leaves                  Poisonous chemicals
                flowers, shoots
                                       under the surface.                    such as imidacloprid,
                and leaves
                                       Kill young shoots, the                fipronil and
                                       carrier virus disease.                profenofos spraying
                                                                             when necessary and
                                                                             repeated 7 days
                                                                             later.
Chili   Hama                           •    ues suck fluid leaves,   •   Poison pests such
        (Polyphagotarsonemu                 leaf berkerinting,           as propargit used
        s latus, Tetranychus                small, pointed and           when necessary and
        sp.) Very small                     curved down.                 repeated 7 days
        (<1 mm) white                                                    later.
                                       •    Texture thick and
           Yellowish
                                            brittle leaves, shoots
                                            to kill.
        White fly                  •   Insects are sucking the       •   insecticide such as
        (Bemisia tabaci)               fluid from the surface of         profenofos (10 HST),
Chili
        • Small, white wings           the leaf. Leaves turn             prothiophos (7 HST),

        and body.                      yellow and sometimes              furathiocarb (7 HST),
                                       there are spots klorosis.         malathion (7 HST), oil

                                   •   This insect produces              and white imidaclorprid

                                       sweets that encourage             (7 HST) effective control

                                       fungal growth-kan soot            of these flies. Insecticide

                                       on the surface of leaves.         sprays can also prevent

                                       This prevents fungus              the spread of white fly
                                                                         virus spread as
                                   •   affect the process of
                                                                         permanent (persistent).
                                       photosynthesis and
                                       plant growth.




                                       43
Appendix 2

WHAT KULTUR In-Vitro
Cultured in-vitro culture covering protoplas, cells, tissues and organs in the media
and the right environment and aseptik.


USE IN TECHNICAL-vitro
1. Plant breeding clones quickly
2. Liberation of plant disease
3. Method storage janaplasma
4. Embryo rescue
5. In plant breeding techniques such as culture specific haploid
6. Production such as culture haploid
7. Production of compounds, such as drugs, enzymes, flavorings and others.


BENEFITS OF IN-vitro reproduction
1. A high reproductive rate and fast
2. Quality high yield and uniform
3. Plant materials can be obtained at any time
4. Production of planting material more easily controlled
5. Planting material easily brought to any
growth crops that can be difficult to breed in common


CROP PRODUCTION PROCESS MATERIALS
Phase 1 - Preparation of plant materials
Phase 2 - Phase inductance
Phase 3 - Phase reproductive shoots
Phase 4 - Phase pengakaran shoots
Stage 5 - Stage management in the home nursery and natural conditions


PROBLEMS AND TECHNICAL doubts about the In-Vitro
1. Melibatan the high cost
2. Memerlikan expertise of high
3. Mutation




                                         44
EKSPLAN
Ekspaln the tree that is separated from the parent tree, which is used to start the
culture in-vitro. In theory any plant can be used as part eksplan, for example the
end of shoots, stems, leaves and roots. Parts are used depending on the type of
crops, methods and goals of the culture.


MEDIUM
Eksplan separated from the parent tree interrupted supply elements essential to
life and further growth. Therefore, elements of this growth medium supplied
through (artificial). Medium can be solid or liquid medium. Generally in-vitro
culture medium consisted of the following components:
1. Macro nutrients
2. Micro nutrients
3. Sugar
4. Vitamins
5. Growth regulators
6. Natural extracts
7. Pemejal agents and other


MEDIUM MS (MURASHIGE AND SKOOG, 1962)


A. Macro nutrients
1. Ammonium Nitrate
2. Potassium Nitrate
3. Potassium Phosphate
4. Magnesium Sulfate
5. Calcium Klorid


B Micro Nutrients
1. Acid Borik
2. Manganum Sulfate
3. Zinc Sulfate
4. Potassium Iodid
5. Sodium Molibdat



                                         45
6. Copper Sulfate
7. Cobalt Kloride


A. Iron
    1. Iron Sulfate
    2. Dinatrium ethylene Diaminatetra Acetate (Na EDTA)


    B Vitamins
    1. Thiamine
    2. Piridoksin
    3. Nikotonoc acid
    4. Glycine


    C. Material growth regulators (hormones)
    1. Auxin
    2. Sitokinin
    3. Gibberelin


    D. Natural extracts
    1. Coconut water
    2. Tomato juice, fruit juice and other


    E. Agent pemejal
    1. Agar-agar
    2. Agar-agar Artificial (Gelrite)


    F. Other additional
    1. Mio-Inositol
    2. Activated charcoal
    3. Antibiotics


PHYSICAL FACTORS that affect tree growth
1. Temperature - around 25 - 28oC
2. Light - 1.000 to 3.000 lux in the room growth from 12 to 16 hours
3. medium pH - 5.7 - 5.8 for MS medium
4. ventilation - the volume of medium in about 20 - 25% of the volume of container.




                                             46
GENERAL PRACTICES KULTUR In-Vitro


1. THE ASEPTIK
Among the most important thing in the work culture in-vitro is cleanliness. The
aim is to maintain the status sterility (bacteria free) culture in the former. It is
important to maintain cleanliness of the room, especially growth. Use
desinfaktant like Dettol to wipe floor room from time to time growth is
encouraged. Culture that is often contaminated diautoklafkan separately before
the former kutur washed for reuse.


2. SUB-KULTUR
Culture should be transferred to new medium when the food supply in the
medium has been exhausted, a different medium when needed or when
changing the nature of medium (dry, changing colors). Discoloration medium
showed production of hazardous waste to the tissue. Normally, each
pengsubkulturan done 2 to 6 weeks.




                                           47
Appendix 3


DEBT ORKID
MEDIA: VACIN AND WENT (VW1) 1949 (modification)
      KOMPONEN         Total for 1 liter   Total for   Total volume     Total volume
                                             stock     of stock used   of stock used
                       Media (g/liter)     (g/100ml)     (m/liter)        (m/liter)
1 NUTRIEN MAKRO
A. Trikalsium fosfat          20              2             10               1
   Ca3(PO4) + HCl
B. Kalium Nitrat            52.2             5.25           10             26.1
   KNO3
C. Asid monokalium
   fosfat                     25              2.5           10             12.5
   KH2PO4
D. Magnesium sulfat           25              2.5           10             12.5
   mgSO4 7H2O
E. Ammonium sulfat            50              5             10               25
   (NH4)2 SO4
2 NUTRIEN MIKRO
F. 1)Manganum Sulfat        0.075           0.0075          10            0.0375
   2) Boric Asid           0.056 g          0.0056                         0.028
   3) Molybolic Asid       0.016 g          0.0016                            -
   4) Cuprum sulfat        0.04 g           0.004                          0.02
   5) Zink Sulfat          0.33 g           0.033                          0.165
3 FERUM
G. 1) NaEDTA                3.73            0.373           10             1.865
   2) Ferum Sulfat
    FeSO4 7H2O              2.78             0..78                         1.39
4 OTHER ENHANCEMENTS
  Sukrose                     20
    Coconut water          200 ml                                      Burn Frozen
   Agar-agar Gelrite       1.4 gm/liter
   Activate Charcoal      2 gm/liter




                                     48

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MARDI report training

  • 1. STATION MARDI CAMERON HIGHLANDS PETI SURAT 19, PEJABAT POS TANAH RATA, 39007 CEMERON HIGHLANDS, PAHANG. Industrial Training Report Submitted in Partial Fulfillment of the Requirement for the Award of D.Sc (hons), Planting, UiTM Faculty Applied Science University Technology Mara By MARZUQI BIN MOHD SALLEH OCT 2009 1
  • 2. Student’s Declaration I declare that this report is the result of my own works except as cited in the references. The report has not been accepted for any diploma and is not concurrently submitted in candidature of any other diploma. Signature : Name : Marzuqi Bin Mohd Salleh Matrix No : Date : 28 January 2010 2
  • 3. ACKNOWLEDGEMENTS Writing reports has become a practical way unusual for me; an experience not be alone with the blessings of Allah Al-mighty. I have several people to thank for helping me achieve this exceptional performance and extraordinary. First, I have a family, especially my father Mrs Mohd Salleh Bin Ahmad to thank for providing emotional support and inspiration. He continued to encourage me to stay motivated and focused. Secondly, I have a mother Salbiah Binte Mahamud and my family to thank for their extraordinary patience, moral and financial support. In addition, I was really doing my practical training for 5 weeks. I have been conducting, Mrs. Zainal Bin Salleh, all friends and all stations Mardi support have helped me a lot to thank you for doing good deeds to keep running on practical training. Last but not least, I would also like to thank all lecturer practical training, all my friends, other students for their continuous support in the training practical. In short, for every person who has touched my life, and especially those involved in writing reports misery, I thank you. Thank you from my heart policy, because I can’t do this without you. Thank you very much, you are the best! Thank you. 3
  • 4. OBJECTIVES • To educate the DPIM students to become a professional person with the characteristics are credibility, responsible hardworking level. And also are sincerely religious in exploring the earth with responsibility especially in agriculture. • For build the attitude to DPIM students. So that, the DPIM students will have are courage, hardworking and also strong in physically and mentally as well as. Knowledge among DPIM students need to establish. • To develop estate with plenty types of crops as convenience when the student having their practical training. • To develop the landscape / garden that is beautiful and interesting places in the campus not just as a recreation park but also as an education and exposure to the students about method and landscape design that is very interesting. • To upgrade the product produce by this farm by using new method in agriculture as well as a source of income for UiTM Perlis. • Complete the equipment that is use for agriculture for the student convenient to achieve goals and to rise up the student potential in agriculture. • To help general academic department and student affairs in any types of facilities that farm department should have. • To have are responsible, positive attitude and with the highly moral ethics of workers when giving their services. 4
  • 5. CONTENTS Content Page ________________________________________________________________ Student’s Declaration i Acknowledgements ii Objectives iii Content iv – v CHAPTER 1 : COMPANY PROFILE 1-2 CHAPTER 2 : EXECUTIVE SUMMARY 2.1 Programs and Activities 3-5 CHAPTER 3: PERSONAL INVOLVES 3.1 Who are involves 6-8 3.1.1 Supervisors from MARDI CHAPTER 4: EQUIPMENT OR WORK PROCEDURES INVOLVES 4.1 Nature of crops 9 4.1.1 The Chrysanthemum (Kekwa plants) 9 - 10 4.1.2 Tea planting 11 4.1.3 Apple (Malus Domestica) 12 4.1.4 Nursery 13 4.2 Field Maintenances 14 4.2.1 Control of Grass 14 – 16 4.2.2 Pruning 17 4.2.3 Mulching 18 5
  • 6. 4.3 Information 4.3.1 Setting the drip system 19 4.3.2 Technology of Fertigation 20 4.3.3 Soil and water conservation 21 4.3.4 Asexual Propagation 22 4.3.5 Landscaping 23 - 25 4.3.6 Compost 26 - 27 4.3.7 Propagation techniques 27 - 30 4.3.8 Outdoor activities 31 CHAPTER 5: CONCLUSION 32 CHAPTER 6: REFERENCES 33 CHAPTER 7: APPENDIX 34 – 40 Appendix 1 34 – 35 Appendix 2 36 – 39 Appendix 3 40 6
  • 7. CHAPTER 1: COMPANY PROFILE Welcome to Agro Technology Park in MARDI Cameron Highlands The Cameron Highlands countryside is endowed with inexhaustible historical, natural and human riches, generously revealed to the visitor. Its traditional villages, enhance and complement a wonderful natural environment, with their local character, traditional architecture and village planning. The district of Cameron Highlands countryside is endowed with inexhaustible historical, natural and human riches, generously revealed to the visitor. Its traditional villages, enhance and complement a wonderful natural environment, with their local character, traditional architecture and village planning. The MARDI Cameron Highland’s Stations was first established by the British in 1925. It was then known as the Federal Experimental Station. Tea was first introduced in the country in 1925 and these early tea bushes are growing healthy at the station until today. The first tea factory built in 1935 is still capable of processing black tea. The Agro technology Park MARDI Cameron Highlands is located within the station and was officiated by His Royal Highness DYMM The Sultan Of Pahang on 14th of June, 2003. Since then, about 65,000 visitors, visit the park each year. The Agro technology Park is only 1km away from the town of Tanah Rata and easily reaches by car, taxi, bus or even by foot. Among the major attractions are the English Garden, strawberry production, vegetable and cut flower production, green house research and the sale centre. 7
  • 8. Figure 1.0 Office. Map of Agro Technology Part Figure 1.2 all areas in the MARDI Cameron Highlands. 8
  • 9. CHAPTER 2: EXECUTIVE SUMMARY 2.1 Programs and Activities We are 3 members. My group’s members are: 1. Marzuqi Bin Mohd Salleh 2008502409 2. Mohamad Syazwan Bin M. Rosley 2008766593 3. Muhamad Hafiz Bin Shamsudin 2008754211 Date Unit Conductor Activity 18 to 23 Soil and water Pn. Aini Hayati 1. Transplant. Nov.2009 conservation. • The chrysanthemum plant. 2. Weeding. • At the soil and conservation plot. 24 to 25 Cutting, Roses, En.Yazid 1. Weeding. Nov.2009 Orchid Mahiyuddin Management. • At the Gerbera plant. 2. Transplant. • The chrysanthemum plant. 3. To make a simple landscape. • At the orchid house. 30 Nov. to Commercial En. Mustafa 1. Weeding. 04 Dis.2009 Project. Kamal • At the arachinis 9
  • 10. hypogaea L. 2. Soil reparation. 3. To fill up the cocopeat into the poly bag. 4. Medium mixture. 5. Setting up the drip system. 6. Compound clearing. • At the arachinis hypogaea L. 7. Pruning. • At the arachinis hypogaea L. 07 to 09 Dis. Sub-tropical En. Osman sah 1. Planting the orange 2009 project. plant at the orange plot. 2. To make the wedge grafting. 3. Transfer the orange plant at the nursery. 4. To make the cut grafting. 5. To make the marcotting. 10 to 14 Tea plantation En. Muhammad 1. Clearing the morning Dis.2009 and banana Nor glory plant at the tea khaki. plot. 2. Weeding at tea area. 10
  • 11. 3. Visit to Nursery. 15 to 17 Agronomy En.Mohammad 1. Pruning tomatoes Dis.2009 Abid plant. 2. To fill up the cocopeat into the poly bag. 3. Planting the pearl grass at the edible garden area. 21 to 22 Entomology En. Zulkifli bin 1. Weeding. Dis.2009 Mohd Saaid • At the edible garden and clearing that area. 2. Planting. • Planted the vegetable plant into the pots. 3. Pruning. • Tomato plants at the tomatoes plot. 11
  • 12. CHAPTER 3: PERSONAL 3.1 Who are involves 3.1.1 Supervisors from MARDI 1. Name : Puan Aini Hayati binti Abd. Rahman Position : Assistant Research Officer (Q32) Unit : Soil and Water Conservation Status : Married Name of Husband : Jamaludin bin Ismail 2. Name : En. Jamaludin bin Ismail Position : Research Assistant (Q17) Unit : Soil and water Conservation Status : Married Name of Wife : Puan Aini Hayati binti Abd. Rahman 3. Name : En. Yazed bin Mahiyuddin Position : Research Assisstant (Q17) Unit : Cutting, Roses, and Orchid Management Status : Married 12
  • 13. 4. Name : En. Mustafa Kamal bin Hanafiah Position : Research Assistant (Q17) Unit : Commercial Project Status : Married 5. Name : En. Osman Sah bin Md Daud Position : Research Assistant (Q22) Unit : Sub. Tropical Project Status : Married 6. Name : En. Muhammad Noor bin Bahauddin Position : Research Assistant (Q23) Unit : Tea Plantation and Banana Khaki Status : Married 13
  • 14. 7. Name : En. Mohammad Abid bin Ahmad Position : Research Officer (Q41) Unit : Agronomy Status : Married 8. Name : En. Saiful Zaimi bin Jamil Position : Research Officer (Q41) Unit : Entomology Status : Married 9. Name : En. Zulkifli bin Mohd Saaid Position : Assistant Research Unit : Agronomy Status : Married 14
  • 15. CHAPTER 4: EQUIPMENT OR WORK PROCEDURES INVOLVES 4.1 Nature of Crops There are various types of green plants, fruits and vegetables. In Cameran Highlands, plants that live there in the cold temperatures. Therefore, MARDI cameran highlands also provide opportunities for visitors who want to see the various plants that cold weather. 4.1.1 The Chrysanthemum (Kekwa plants) They came from China. They are living ‘Short-day-plant’. They need more extra light at night (>10.5 hours) for a shape of flower. Get the suitable length of the chrysanthemum stem about (100 cm) in time of period and control for slowing the flower to grown up, They need more extra light of 100 watt ± 4 hours at night during in 5 to 8 weeks. Used lamp until the plants grown about 35 cm high for 5 to 8 weeks. A shaped of flower can be seen after 2 to 3 weeks and they harvested in 6 to 10 weeks after switch off the lamp. In one season is about 3 to 4 months. Spray/pompon species were planted >90% in Cameron Highlands than standard species. They are also planting with propagation by cutting and seeds. Popular species are Reagon Sunny, Reagon Purple, Jaguar Purple, Red, Monalisa Yellow, Monalisa White, Dublin Red and Spider White. The technology light with cutting propagation method of Chrysanthemum plants:  The Chrysanthemum plant need more extra night light (Short-day-plant).  After planted the Chrysanthemum tree it needs more extra light of 100 watt ± 4 hours at night. Also, they always need switch on the light during in 5 to 8 weeks after that switch off the lamp in 6 to 10 weeks.  Used extra light every night for : -To get the suitable length of Chrysanthemum stem about (100 cm) in period date. 15
  • 16. -To control for slowing to grown up In plant planting, the transplant is very important because to give plant growth on the soil actual and get direct nutrient and absorb water or air from soil. Normally, in this case they used propagation by cutting. As we know, propagation have more advantages such as to retain the desirable characteristic of the mother plant and increase or multiply the number of plants rapidly. Figure 4.0 Chrysanthemum shows that the way of retransplanting. Figure 4.1 show the place where chrysanthemum tree are planted. To produce the best quality of chrysanthemum plant they need more cool weather and produce the colourful flower. 16
  • 17. 4.1.2 Tea planting Tea planting is a living green leaf in family of Camellia and they came from China, Tibet and North India. There are two varieties such as small leaf and wide leaf. In small leaf variety, known as Camellia sinensis, live well at district of highland in Cameron Highlands. In wide leaf variety, known as Camellia assamica, most live well at district of tropical. In tea planting, there are colorful dark green, small size and white flower. Figure 4.2 show that the current working of the plant weeded control. 17
  • 18. Figure 4.3 show that way of planting in the Cameron Highlands. 4.1.3 Apple (Malus Domestica) The apple thee is temperate plants. Dormant time for winter (<7°C, until > 100 hours) need to promote the flowers. In tropical compound, there are pruning, bend the branch, drop leaves, and hormone spraying (Dormex 3%). Highlands area >800m have potential to planting apple from varieties that need short dormant time (~300 hours) like Anna, Grany Smith and Rome Beauty. After >3 years planted, all leave will be dropping. New leaves and flowers will be growing up after 2-4 weeks. Schedule of dropping the leaves are very importance to do so that the production of flower occur in less rain season (open area). Fruit will mature after 125 days flowering. Produce two time a year (May- June and Nov- Dis.). Figure 4.4 show that the place where apple tree are planted. Figure 4.5 show that way of planting in the Cameron Highlands. 18
  • 19. 4.1.4 Nursery 4.1.4.1 Planting of tea plant. Learn the techniques how to make planting the tea plant in nursery? • Select the suitable stem and free pest or disease. • Length of stem about 5 to 6 cm. • Put the hormone (IAA) at the end of stem. • To planting into the poly bag. • Follow by watering. • All poly bags must be put into the white plastic. Then, close be used to white plastic because they are enough nutrients, enough humidity, and weed controls. • For our information, in that case for tea planting they used to plant by cutting propagation because they are easy grown rapidly. • After 1 year they will transfer to the field. Figure 4.6 show that the place where tea tree are planted. 19
  • 20. 4.2 Field Maintenances • Each plant must have care well to plant a garden so fertile. Care was often each week. 4.2.1 Control of Grass 1. Manual Control -Used the mulching (e.g. plastic, cover crop and etc). -Used the labor energy. 2. Mechanical Control -Used the tractor 3. Biology Control -Used the insect such as bacteria or virus. 4. Chemical Control -Used the chemical spray. -Save cost. 20
  • 21. figure 4.7 show that weeding method with good. Grasses Problem  Grass is no need in plant. It makes trouble to plant.  It challenged between the plant to get nutrient, water, space and light.  Some grasses needs in plants such as Maman Pasir.  The Maman Pasir is insect control.  Cost of grass is 10 % to 35 % than cost of planting. Disadvantages of grass • A challenged with plant. The plant become dwarf and loses profit, it caused the grass more quite grown than plant. • Drain irrigation will become trouble. General Recommendations for Weed Management 21
  • 22.  Likewise with insects, identifying all the different weed types in the farm and understanding their means to survival and their role in agro-ecosystem are important steps towards their management.  It is also essential to adhere to this basic principle in managing weeds. Which is minimizes to competition between the weed and the cultivated crop.  For weed control, the following methods and materials could be used. figure 4.8 show the weeds that have been extracted by hand. 1) Cultural Practices -The use of cultural practices, which limit weed development include manual weeding around the base of the plant and on the beds, crop rotation, forking, digging and burying, rot ovation, close spacing and the use of ground covers. 2) Mulching -The use of dried grass mulch on the beds and regulated use of plastic mulch to cover the surface of the beds before planting help to reduce weed infestation. 22
  • 23. 3) Physical Control -In some instances, flame weeding could be used. • Weeds are major problem in any farm. They need to be managed well even at an early stage of cultivation; otherwise the following problems will result in: a. High costs for labor and time taken for management (almost 50%) to remove these weeds; b. Adverse effects on crops when they start to complete for water, nutrients and light. They can stunt crop growth and cause loss of yield. c. Harboring of pest and pathogens, such as Cleome rutidospema, which is host of flea beeties, H. undalis and P. xylostella. • To avoid the above problems, early interventions are necessary to prevent the build-up of high weed populations, especially during rainy seasons. The major weed species found in a vegetable farm include both broadleaf and grasses. The major grassy weeds include nutgrass, Eleusine indica, Cyperus rotundus and Paspalum conjugatum. The broadleaf weeds commonly found are borreria latifolia, C. rutidospema, Asystasia gangetica and Amaranthus spinosus. Naturally, the type of weed species found in an area depends on the inherent soil conditions and agronomic practices typical of the farm. 4.2.2 Pruning • Pruning is the activity of removal of a plant to improve its appearance and health and to control it growth and shape. Purposes of the pruning are: • Removed the dead / diseased portion /part. • To get the shape of the plant-landscape. • Sanitation. 23
  • 24. The different between pruning and thinning is: • Pruning is the removal of portion of the plant to improve its appearance and health and to control it growth and shape whereas pinching is the removal of the terminal shoot on each branch of the flower and allows the lateral shoot to develop, thereby creating fuller plant. figure 4.9 shows the results of clipping trees and a shady. 4.2.3 Mulching • Is the process of spreading generally organic plant materials between and around the plants to cover the soil. Mulches reduce soil moisture loss, reduce soil temperatures, control weed and increase organic matter content after decoying. Example of materials used: Leaves, rice straws, grass clippings, cocoa beans, sawdust, oil palm shell and ground fir bark. • A plastic Mulching used to site location plants as to control the weeds. 24
  • 25. White color: A minimizes attack from insect to perch under leaf. • Black color: For soil treatment. -To kill the pathogen from soil. figure 4.10 show the mulching completed installed. figure 4.11 to show the installation is in progress. 4.3 Information 4.3.1 Setting the drip system In all the plants, flow system is very important to planting because to support the plant growth well. From planting on flower bed (planting Chrysanthemum) it used the drip system. What we did it? Firstly, from the drip system has loose. So, we 25
  • 26. learn the technique How to taut the rope. Most importantly, we should have bend crossing string well. It’s because to support the drip system flow stay in well position and easy to manage. • Netting is used to support the Chrysanthemum plant. It needs to move up when the plant starts increasing to grown up. Figure 4.12 show how to made a setting the drip system 4.3.2 Technology of Fertigation Fertigation is a combination of two words ‘Fertilization’ and ‘irrigation’. The concept of this system is to deliver fertilizer in form of a solution to the root zone through the irrigation system. This technology can be the applied in the soil or soilless, in the open field or under protective structive structure (PS), However, 26
  • 27. this system recommended implement in soilless media under prodective structure to reduce the risk of soil borne disease and disease of leaves, shoots and fruit on the plants. In addition, PS can also avoid the fertilizer solution given to the plant to be too liquid. Therefore, Fertilization is not effective. Media used is coconut coir. Dust or better know as cocopeat because it is cheap and easily can obtained from local source. Equipment used in this system is stock tanks, solution tanks, filter, main pipe, delivery pipe, micro tube and dripper. Electrical power pump may be used as an alternative to the gravity method to deliver the fertilizer solution to the plant if insufficient water pressure or to irrigate a vast area of the farm. The used of the limer will make the fertigation system more efficient in terms of management of the fertilizer solution to the plant. Application of fertigation of fertigation system has many advantages, which may reduce the operation cost, improve quality and productivity, time saving, reduce the number of labour and labor cost and increase the efficiency of field management. Besides, fertilizer and water are effectively used. Soilless media can reduce the risk of soil borne disease and avoid the soil pollution caused by salt accumulation. Fertigation is a clean system, odorless, environmental friendly and crop such as tomato, red chili, cucumber, zucchini, eggplant, melon, capsicum and strawberry. 4.3.3 Soil and water conservation Soil erosion by water is main soil degradation process in CH. Erosion process is detachment and transportation of soil particles by erosion agent (i.e water and wind) and ended with deposition. Erosion carried together with nutrients, and pesticides resulting in eutrophication (nutrients enrichment in rivers and lakes). Objective of the soil and water conservation plot is to show the effectiveness of 27
  • 28. cover crop and horizontal arrangement of soil bunds. Visitors are exposed to the techniques in measuring soil loss, surface runoff and rainfall. Rhizotron is constructed to show the root growth of daylily which is comparable to vetiver grass is recommended by FAO to control erosion on slopes. There are six construct plots in soil and water conservation area: 1. Cabbage (Capitata) 2. Kum Chum (Hemeocalis Fulva) 3. Cover Crop (Desmodium sp.) 4. Vetiver grass (Vetiveria zizanoides (L) Nash ) 5. Cabbage (Capitata) 6. Lycium Sinensis (Kau Kei) Figure 4.13 show that how to use the clinometer.  Slope for soil and water conservation plot is 5°.  Used the clinometer for determine or measuring the slope. In researched, the Desmodium sp. is the best reducing in soil erosion. 4.3.4 Asexual Propagation • Is the reproduction of plant which is not involved the fusion of gametes. The propagation utilizes the vegetative parts. 28
  • 29. Part of plant can be used in asexual propagation.  Leave  Stem  Root  Embryos  Pollen grains  Shoot tip  Callus Purposes: • To retain the desirable characteristic of the mother plant. • To increase or multiply the number of plants rapidly. • To shorten the bearing age of plant, especially in fruit trees. • To prevent the plant species from being lost or extinct. • To produce superior strains and disease-resistance plant that will be best suitable under given climate and soil condition. 29
  • 30. Figure 4.12 show that how to made the wedge and marchoting methods. 4.3.5 Landscaping • Is the art of purposely changing the natural features that exist out-of-doors, with the intention of making the environment more attractive or functional. This is done to meet the extended needs for man for his physical needs, mental health and comfort. Functions of plants in Landscape design. Contribute to landscaping in two major functions: a) Plants Physical Function: -Control Erosion -Soothes the climate -Provide screening from light, wind and from unwanted views -Create a form of direction -Protect wild-life b) Plants Esthetic Function: -Create spatial effect -Planning views -To soften the concrete effect on the environment -To connect concrete and structure with the ground effect -Creating unity effect on various elements in particular area 30
  • 31. figure 4.13 show the landscape area. Figure 4.14 show the road landscape. 31
  • 32. figure 4.15 show the cuctus garden area. As we know, Plants may be propagated under two (2) general categories: Sexual and vegetative or asexual propagation. Sexual Propagation • Is the common method of reproduction and multiply of plants. This is usually done with the use of seeds and spores. Garden vegetable like tomato, eggplant, radish, carrot, mustard and legume crops (Cawpea, Munyo and Peas) are generally propagated by seeds. Fruits trees such as avocado, santol, jackfruit, guava, mango, etc. are also propagated by the use of seeds, although in some cases, the resulting plant may not come true to type and to not have the same characteristic as the parent plants. • Seeds are still the most economical and fastest means in the reproduction of plants. However, some plants like fruit trees produced from seeds very in their characteristics. Hence, we cannot always expect springs or progenies. Ferns and mosses are by natural propagation by spores. Asexual Propagation • Is the development of new plant natural or artificial with the use of seeds, since it does not involve the function of sex, it is commonly referred to as asexual. 32
  • 33. Disadvantages: • There is possibility of disease being transmitted from the parent to the new individual plant. • Some source of certified scion or buds sticks is a usual problem. 4.3.6 Compost What is Composting?  Zucconi and Berthold (1987)defined composting as a controlled bioxidative process that: i. Involved a heterogeneous organic substrate in the solid state. ii. Evolves by passing through a hemophilic phase and a temporary release of phototoxic and ; iii. Leads to production of carbon dioxide, water minerals and a stabilized organic matter (compost). Factors Affecting Composting Process • Micro-organism • Carbon : nitrogen ratio (C/N ratio) • Particles size 33
  • 34. Aeration • Moisture • Temperature • Size of heap Cube Box Cocopeat • When wallowed into the water and they are also to be enlarge used to fill up into the poly bag are about 10 poly bags. Figure 4.16 show the Cube Box Cocopeat. Other Benefits of compost are: • It stores and slowly releases some nutrients, for some effective use to the plants. • The organic matter is increased, thereby the root structure, especially the root hairs of the plants are increased. • It balances the pH of the soil by acting as butter. • By recycling wastes into compost, the environment becomes more sustainable. • It attracts earthworms and soil organisms that are responsible for making the soil a ‘living’ soil. • It reduces soil erosion • It suppresses certain soil borne diseases • It reduces reliance on petroleum based fertilizers 34
  • 35. Manage Labour • Weed control • Organic fertilizer application • Compost making • Crop production measures • Post harvest handling and marketing 4.3.7 Propagation techniques • Are the studies of process of extending the life of a particular species of plant such as propagation by grafting like wedge grafting, and whip or tongue grafting. Whip or Tongue Grafting • This method has districts advantage of allowing a much greater area of cambial contact than either cleft or saddle grafting. Procedure: • Start in the same manner as cleft or saddle grafting • After cutting back the rootstock, make a smooth slanting cut about 3-5 cm long where you intend to make the graft • About 1/3 from the cut surface, make a downward cut so it would form some sort of a tongue that point upward • Make similar but reverse cut on the scion • Fit them together making sure their tongues interlock snugly and bind with a plastic tape. Method of Artificial propagation 35
  • 36. Cutting Is a detached portion of the selected mother plants intended to be multiplied, of which at least one-third to one-half of the length is inserted in the soil or rooting media with the sole purpose of producing new plant. Cuttings may be divided into tree classes:  Root cuttings made from matured roots as in the case of rimas.  Leaf cuttings as in Begonia  Stem cuttings soft wood cuttings may either be made from young and immature parts of the plants as in the case of Gumammela, San Francissco and Papua. Hardwood cuttings are made from matured twigs of the plants intended to be produced as in the case of Bougainvillea. Lately, success has been made in producing rooted Chico and citrus with the use of cuttings dipped in root hormone solution. Factors to be Considered in Grafting The weather conditions and the stage of plant should be considered for the success in grafting. Grafting may be done at the beginning of the dry season and rainy season. The scion should be well-prepared from selected pedigreed trees, the characteristics of which are to be perpetuated. In the same manner, the stock should be healthy, vigorous and free from diseases and insect pests. Basic Requirements of Plant Life 1) Water 2) Energy 3) Air 4) Medium Water  Water enables all the major metabolic processes within the plant.  Water acts as a solvent to convey nutrients, foods and other substances throughout the plant. 36
  • 37. Water provides plant cells with their internal Turgor Pressure.  The quantity and quality of water in important -Too much water in the soil can cause the plant to suffocate. Wet roots are more susceptible to rot or mold (kulat). -Too little water will result in loss of turgidity, wilting. Metabolic processes will be reduced, leading to growth retardation and possible death. -Water can be unsuitable for plants if it contains: Pollutants such as herbicides; high salinity: mud or silt. Marcotting • Is the process of inducing Brach or twigs to produce roots while still attached to the parent plant. Procedure: • Firstly, to prepare all equipment those like knife, suitable plant, medium, hormone and etc. • To select the suitable plant. • Make a girdle around the branch about 1 to 2 cm wide or depend on the size of the branch. • Scrape off the cambium layer completely to prevent the re-growth of the bark. It may help to apply a rooting compound to the exposed the stem. • Wrap the cut surface with about a handful of medium. If the medium is too wet, the stem may decay. • Wrap with a piece of plastic film about 15 × 20 cm around the ball of medium and tie all around with cotton twine. The plastic film keeps down loss of moisture from the medium. • As soon as you see enough roots throughout the plastic film, cut the marcotted branch from the mother plant. It may be from 2 to 12 months after marcotting depends on the crop. 37
  • 38. Cutting may be done in gradual basis until if attain sufficient roots to live by itself as indicated by a new flush of growth (a critical period for the new plant). figure 4.17 marcotting completed. 4.3.8 Outdoor activities We have an opportunity there with staff Mardi mountain climbing. 38
  • 39. CHAPTER 5 : CONCLUSION In conclusion, we have got more information and knowledge in MARDI Cameron Highlands. One of all, many thank to MARDI Cameron Highlands organizers because they were accepted us as practical training students there. As long as 5 weeks we have completely done our practical training, also we have 7 units followed such as Ms. Aini Hayati bt Abd Rahaman in unit Soil and Water Conservation. With her, we have learned about Chrysanthemum plant. Secondly, followed by Mr. Yazed bin Mahiyuddin in unit of Cutting, Roses, Orchid Management. In his unit, we learned that how to make transplant the Chrysanthemum plant and pruning at Gerbera plant. Thirdly, we followed En. 39
  • 40. Mustafa Kamal bin Hanafiah in unit of commercial project. With his, he teaches to us how to make that filled up cocopeat into the poly bags, put mulching on the bed and make medium mixture (cocopeat:Organic fertilizer)(1:1,5:1). We learned about organic plants there are no need chemical matters and they use biological control for maintenance. After that, we have followed Mr. Osman Sah in unit Sub Tropical Project. With his, we learned about propagation techniques such as marcotting and cutting. We have got experience to make marcotting and cutting. It was interesting for us to learn. Then, we have followed Mr. Mohammad Nor in unit Tea Plantation. We were learned to pruning and weeding. Morning Glory is challenging between tea plant and Morning Glory, so they need to cut off. Propagation for tea is cut grafting. Tea plant has live for many years. After that, we have followed Mr. Mohammad Abid in unit Agronomy. We have put mulching on the bed, filled up the cocopeat, pruning and harvest tomatoes. Lastly, we have followed Mr. Saiful in unit Entemology. In his unit, we have learned about pest and disease for example, there are four main insects in Cameron Highlands such as Plutella Xylostella, Leaf Minor, Red Spider Mites and White Fly. For control component, there is Integrated Pest Management (IPM) such as parasite, predator, bacteria and virus. Finally, we have apologies to all organization. We hope all those knowledge we can use for next time. All profits that come from God and all come that from our lack. Virtually all gains and losses have come from Allah S.W.T. 40
  • 41. CHAPTER 6: REFERENCES Derector, infomardi : Chrysanthemum, Info Center Argo Technology, MARDI, Cameron Highlans. Derector, infomardi: tea, Info Center Argo Technology, MARDI, Cameron Highlans. Derector, infomardi: fruits, Info Center Argo Technology, MARDI, Cameron Highlans. En. Zulkifli bin Mohd Saaid, A. R. (2009), Agronomy, MARDI Cameron Highlands.Pahang. Aro technology part, welcome to Cameron HighlandsSelamat, Pahang. Puan Aini Hayati, A.R. (2009), Sistem Fertigasi, Bahagian Pengurusan dan Pemuliharaan Sumber Tanah, MARDI Cameron Highlands. Prof Rasyid: Landscape AGR 112, propagation, UiTM Arau, Perlis. 41
  • 42. CHAPTER 7:APPENDIX Appendix 1 Crop pests Major insect pest attack should be given particular attention to plants grown under the protective structure of plants. Among the main insect pest, the type of damage and control methods for plant tomatoes, chilli and melon are as follows. Plant Pest Damage control Chili Afid (Aphis gossypii) • Tues suck fluid • Mulching plastic leaves, young leaves shine can prevent • Flea leaf green wrinkled landing afid, reduce kehijau parents, berkerinting the number of afid, there below the produce klorosis can further reduce surface of the spots, stunted fruit disease virus leaf trees and attacked infection. virus disease • natural enemies such as tortoise beetles can help reduce the number of afid. Selected chemical poisons such as profenofos and imidacloprid spraying when necessary and repeated 7 days later. 42
  • 43. Chili Kutu Trips (Thrips • Tues suck fluid leaves, • mulching plastic sp.) leaves become smaller shine prevent and and privation, wrinkled reduce the number • Insects fine, and curved tapering of early attacks. yellow, found in upward, brown leaves Poisonous chemicals flowers, shoots under the surface. such as imidacloprid, and leaves Kill young shoots, the fipronil and carrier virus disease. profenofos spraying when necessary and repeated 7 days later. Chili Hama • ues suck fluid leaves, • Poison pests such (Polyphagotarsonemu leaf berkerinting, as propargit used s latus, Tetranychus small, pointed and when necessary and sp.) Very small curved down. repeated 7 days (<1 mm) white later. • Texture thick and Yellowish brittle leaves, shoots to kill. White fly • Insects are sucking the • insecticide such as (Bemisia tabaci) fluid from the surface of profenofos (10 HST), Chili • Small, white wings the leaf. Leaves turn prothiophos (7 HST), and body. yellow and sometimes furathiocarb (7 HST), there are spots klorosis. malathion (7 HST), oil • This insect produces and white imidaclorprid sweets that encourage (7 HST) effective control fungal growth-kan soot of these flies. Insecticide on the surface of leaves. sprays can also prevent This prevents fungus the spread of white fly virus spread as • affect the process of permanent (persistent). photosynthesis and plant growth. 43
  • 44. Appendix 2 WHAT KULTUR In-Vitro Cultured in-vitro culture covering protoplas, cells, tissues and organs in the media and the right environment and aseptik. USE IN TECHNICAL-vitro 1. Plant breeding clones quickly 2. Liberation of plant disease 3. Method storage janaplasma 4. Embryo rescue 5. In plant breeding techniques such as culture specific haploid 6. Production such as culture haploid 7. Production of compounds, such as drugs, enzymes, flavorings and others. BENEFITS OF IN-vitro reproduction 1. A high reproductive rate and fast 2. Quality high yield and uniform 3. Plant materials can be obtained at any time 4. Production of planting material more easily controlled 5. Planting material easily brought to any growth crops that can be difficult to breed in common CROP PRODUCTION PROCESS MATERIALS Phase 1 - Preparation of plant materials Phase 2 - Phase inductance Phase 3 - Phase reproductive shoots Phase 4 - Phase pengakaran shoots Stage 5 - Stage management in the home nursery and natural conditions PROBLEMS AND TECHNICAL doubts about the In-Vitro 1. Melibatan the high cost 2. Memerlikan expertise of high 3. Mutation 44
  • 45. EKSPLAN Ekspaln the tree that is separated from the parent tree, which is used to start the culture in-vitro. In theory any plant can be used as part eksplan, for example the end of shoots, stems, leaves and roots. Parts are used depending on the type of crops, methods and goals of the culture. MEDIUM Eksplan separated from the parent tree interrupted supply elements essential to life and further growth. Therefore, elements of this growth medium supplied through (artificial). Medium can be solid or liquid medium. Generally in-vitro culture medium consisted of the following components: 1. Macro nutrients 2. Micro nutrients 3. Sugar 4. Vitamins 5. Growth regulators 6. Natural extracts 7. Pemejal agents and other MEDIUM MS (MURASHIGE AND SKOOG, 1962) A. Macro nutrients 1. Ammonium Nitrate 2. Potassium Nitrate 3. Potassium Phosphate 4. Magnesium Sulfate 5. Calcium Klorid B Micro Nutrients 1. Acid Borik 2. Manganum Sulfate 3. Zinc Sulfate 4. Potassium Iodid 5. Sodium Molibdat 45
  • 46. 6. Copper Sulfate 7. Cobalt Kloride A. Iron 1. Iron Sulfate 2. Dinatrium ethylene Diaminatetra Acetate (Na EDTA) B Vitamins 1. Thiamine 2. Piridoksin 3. Nikotonoc acid 4. Glycine C. Material growth regulators (hormones) 1. Auxin 2. Sitokinin 3. Gibberelin D. Natural extracts 1. Coconut water 2. Tomato juice, fruit juice and other E. Agent pemejal 1. Agar-agar 2. Agar-agar Artificial (Gelrite) F. Other additional 1. Mio-Inositol 2. Activated charcoal 3. Antibiotics PHYSICAL FACTORS that affect tree growth 1. Temperature - around 25 - 28oC 2. Light - 1.000 to 3.000 lux in the room growth from 12 to 16 hours 3. medium pH - 5.7 - 5.8 for MS medium 4. ventilation - the volume of medium in about 20 - 25% of the volume of container. 46
  • 47. GENERAL PRACTICES KULTUR In-Vitro 1. THE ASEPTIK Among the most important thing in the work culture in-vitro is cleanliness. The aim is to maintain the status sterility (bacteria free) culture in the former. It is important to maintain cleanliness of the room, especially growth. Use desinfaktant like Dettol to wipe floor room from time to time growth is encouraged. Culture that is often contaminated diautoklafkan separately before the former kutur washed for reuse. 2. SUB-KULTUR Culture should be transferred to new medium when the food supply in the medium has been exhausted, a different medium when needed or when changing the nature of medium (dry, changing colors). Discoloration medium showed production of hazardous waste to the tissue. Normally, each pengsubkulturan done 2 to 6 weeks. 47
  • 48. Appendix 3 DEBT ORKID MEDIA: VACIN AND WENT (VW1) 1949 (modification) KOMPONEN Total for 1 liter Total for Total volume Total volume stock of stock used of stock used Media (g/liter) (g/100ml) (m/liter) (m/liter) 1 NUTRIEN MAKRO A. Trikalsium fosfat 20 2 10 1 Ca3(PO4) + HCl B. Kalium Nitrat 52.2 5.25 10 26.1 KNO3 C. Asid monokalium fosfat 25 2.5 10 12.5 KH2PO4 D. Magnesium sulfat 25 2.5 10 12.5 mgSO4 7H2O E. Ammonium sulfat 50 5 10 25 (NH4)2 SO4 2 NUTRIEN MIKRO F. 1)Manganum Sulfat 0.075 0.0075 10 0.0375 2) Boric Asid 0.056 g 0.0056 0.028 3) Molybolic Asid 0.016 g 0.0016 - 4) Cuprum sulfat 0.04 g 0.004 0.02 5) Zink Sulfat 0.33 g 0.033 0.165 3 FERUM G. 1) NaEDTA 3.73 0.373 10 1.865 2) Ferum Sulfat FeSO4 7H2O 2.78 0..78 1.39 4 OTHER ENHANCEMENTS Sukrose 20 Coconut water 200 ml Burn Frozen Agar-agar Gelrite 1.4 gm/liter Activate Charcoal 2 gm/liter 48