.

1. Maria Camila Florez Carmona
Pontificial Bolivarian University
III Semester

2. INTRODUCTION
 Salmonella is a highly gram-negative bacterium from the Enterobacteriaceae group, which is
one of the causes of entenc diseases such as salmonellosis and diarrheal infections in
humans worldwide.

3. GENERAL OBJECTIVE
Analyze if the Salmonella antibacterial Rhs polymorphic
toxin inhibits translation through ADP-ribosylation of EF-Tu
P-loop

4. METHODS
 PCR: It allows the rapid production of millions to billions of a specific segment of DNA, which can
then be studied in greater detail. PCR involves the use of short synthetic DNA fragments, called
primers, to select a segment of the genome to be amplified, and then multiple sessions of DNA
synthesis to amplify that segment.
 WESTER BLOD: It is a test that evaluates a reaction between antigen and antibody. I do an
electrophoresis, separate proteins and identify them with specific antibodies to see if they are
present. It was done to evaluate a toxin so they evaluated a protein present.

5. METHODS
 SDS-PAGE: This process, the separation is a function of molecular mass, which allows to determine
the molecular weight of the proteins. For this purpose, the electrophoretic mobility of the protein of
unknown molecular weight is compared with that of reference proteins of known molecular weight.
 TOXICITY ESSAY: determination of the effect of a material or mixture on a selected group of
organisms under defined conditions. It measures the proportions of organisms affected (quantal
effect) or the degree of effect (graded) after exposure to the sample.

6. DISCUSSION
AUTHOR What the main author says It’s Okey?
Kjeldgaard,M The peptide nitrogen of the D21
residue forms a hydrogen bond
with the oxygen of the 􏰀-
phosphate of the GDP
Ok
Maracci,C Indeed, substitutions of the EF-Tu D21
residue have been shown to decrease
the GTPase rate of the EF- Tu–tRNA–
GTP ternary complexes in the
preprogrammed ribosomes
Ok
Vetter,I.R. The D21 residue is located on the P-
loop––a highly conserved motif found
in cellular AT- Pases and GTPases and
responsible for coordinating the
nucleotide phosphates
Ok

7. RESULTS
FIGURE 1.E FIGURE 3

8. RESULTS
FIGURE 5B
FIGURE 4E

9. CONCLUSIONS
 Molecular biology influences the study of the pathogenesis of diseases, this is important because
we can understand the mechanisms and new treatments for different diseases.
 Molecular biology is important in medicine as it participates in the production of new therapeutic
agents to treat different pathologies.