1. PREDICCION DE GENES CON GENSCAN José María Hidalgo Utrera Joan Miquel Fuster Mollá Ana Isabel Martínez García
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5. Conocimientos básicos exon exon start donor acceptor intron CDS UTR UTR Región reguladora EXON 1 EXON 2 EXON 3 EXON 4 EXON n Región reguladora PROMOTOR 3` Intrón 1 Intrón 2 Intrón 3 Secuencia que no se traduce Secuencia que no se traduce 5`
12. Modelo general Signal Models State length distributions Transcriptional Translational signals Splice Signals MDD Acceptor splice Site model Exon models Reverse-strand states Algorithmic issues Initial, transition probabilities HMM
splice site determination is a crucial part of determining gene structure. All good programs take these short motifs into account. INTRON: RNASPL: A V. Solovyev program ( h ttp://dot.imgen.bcm.tmc.edu:9331/gene-finder/gf.html) Recognition of exon-exon junctions in cDNA. Prediction is performed by linear discriminant function combining characteristics describing tipical sequences around exon-exon junctions. SPLICEVIEW: The program is based on prediction of splice signals by classification approach (a set of consensuses). It is based on two main assumptions: 1) The high frequency of some nucleotides in definite site positions reflects a functional importance to preserve the nucleotide in this position. 2) Nucleotides of different site positions are considered to be mutually dependent, thus forming the structure which might be recognised by some particle. (http://l25.itba.mi.cnr.it/~webgene/wwwspliceview_help.html)
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