Alta Kadarusman - Vertebrates Plenary

The number of freshwater species in the world is quite small when compared with the vast number of marine species. There are several marine forms which are penetrated brackish and freshwater but remain unable to breed there while others have adapted sufficiently to remain for their entire life span. Polychaetes are commonly found in Indian estuaries, among 152 species recorded, 119 species from the east coast, 10 species from both east and west coasts. A total of 8 species were identified along Thenkasi District. Macro benthic polychaetes highlighted the presence of indicator species at all stations. Most of the benthic studies explored that Nereididae is the dominant species. The polychaetes observed throughout this study were a similar size and this is a clear indication of extreme disturbance imposed on the sediment. The present findings show macrobenthic polychaete diversity rich all along the Thenkasi District of Tamil Nadu. S. Balasubramanian | T. Citarasu | S. Lazarus | A. Renu "Species Diversity of Polychaete Worms from Some Selected Freshwater Environment of Thenkasi District, Tamil Nadu" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-5 , August 2019, URL: https://www.ijtsrd.com/papers/ijtsrd26516.pdfPaper URL: https://www.ijtsrd.com/biological-science/zoology/26516/species-diversity-of-polychaete-worms-from-some-selected-freshwater-environment-of-thenkasi-district-tamil-nadu/s-balasubramanian

Extensive cryptic diversity in Indo-Australian rainbowfishes revealed by DNA Barcoding

Lifestyle Business

Interest for Rainbowfish ,[object Object],30,000 years ago 50,000 years ago Pearl for aquarium trade Diversity & Systematic confusion Threats on Papuan Melanotaeniids ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],(15) (61)

Objectives ,[object Object],[object Object],[object Object]

Study area and sampling localities 105 Localities 35 IRG specimens Iriatherina Glossolepis Chilatherina Melanotaenia 4 Genera 53 Nominal Species New Guinea Vogelkop Cape York Port Moresby

Methods and Analysis Expedition 2007-2010 Vogelkop Peninsula Half Northern New Guinea Breeding Specimens Commercial sources Rainbowfish Association-IRG Genomic DNA Extraction + PCR + Sequencing Sequences Alignment BOLD module Divergence: K2P Graphic representation : NJ Tree Deposited: GenBank + BOLD Datation Geology-based calibration + some published Mitochondrial molecular clock Phylogenetic Species 1. validate 2. define Diagnostic morphological characters

Results and discussion ,[object Object],[object Object],[object Object],[object Object],Highlight four major clades : I: Vogelkop Peninsula New Guinea mapsof.net Include 9 australian species IA: Main Vogelkop IB: Bomberai II : Kuweri Lengguru III : South NG + Australia IV : North NG

Mixed COI cluster ,[object Object],[object Object],[object Object],New Guinea Vogelkop Australia

Cryptic lineages detected ,[object Object],[object Object],[object Object],Vogelkop peninsula Bomberai Lengguru Ayamaru Nominal species Cryptic lineages

Mixed conspecific & Congeneric Min. K2P = 0 Genetic divergence and taxonomic levels Overlap between within and among species K2P distances (closely related species) Genetic Divergence : Congeneric 14x higher than Conspecific Fig. 1. Distribution of the K2P distances 350 individuals 69 species analyzed

Fig. 2. Distribution of the K2P distances to NN Intraspecific divergence Overlap between within species genetic distances and distances to NN (closely related species)

Large scale pattern of genetic divergence Include 9 australian species Clade III Clade IV Clade II Clade I ,[object Object],[object Object],[object Object],[object Object]

[object Object],Relationship among clades Clade IA Clade IB Clade II Clade III Clade IV ,[object Object]

[object Object],[object Object],Datation ,[object Object],[object Object],11.4 myr 8.8 myr Min.subs R: 27.4 and 21 Myr Canonical ‘fish’ : - Estimated : 11.4 and 8.8 Myr - Fixed: 0.012 subst./Myr Can. Verteb. Subs R: 11.4 and 5.3 Myr Uplift Lengguru Arch : - Fixed : 12 and 9 Myr - Estimated: (0.009 and 0.015 subst./Myr Clade III Clade IV Clade IA Clade IB Clade II Synthetic cladogram among clades (lengguru)

Lengguru Arch Vogelkop and the role important of Lengguru Arch ,[object Object],[object Object],[object Object],Ayamaru plateau Raja Ampat Is

Conclusion ,[object Object],[object Object],[object Object],[object Object],[object Object]

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