This document provides a protocol for extracting ancient DNA from bones and teeth. The method aims to maximize DNA recovery while minimizing co-extraction of PCR inhibitors. Key steps include extracting DNA from bone powder using an EDTA-proteinase K buffer, then purifying the DNA by binding it to silica in the presence of guanidinium thiocyanate. All steps are done at room temperature to reduce DNA degradation. The protocol yields DNA extracts within 2 days and has advantages over other methods such as being quick, scalable, simple to implement, and efficiently removing PCR inhibitors.