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Fish as food
TYBSc Semester VI
(Applied component: Marine science)
Biren Daftary
Biochemical composition of Raw Fish
• Principal contents: protein, fat and water
• Protein = 20%
• Variation in fat and water
• Minerals, vitamins and enzymes
• No carbohydrate except glycogen in liver
• Water = 55 - 83%
Proteins
• Normal 15 - 20%
• In some species < 15% or as high as 28%
• Chains of chemical units linked together to make
a long molecule
• Each unit = amino acid (20)
• Essential in human diet for maintenance of good
health
• Lysine & Methionine are found in high
concentrations in fish proteins
• Skeletal protein = 50 – 60% of total weight of fish
• Protein content classified into following
groups:
a) Albumin fraction: 16 – 22%
b) Protein fraction: 75%
c) Stroma: 3%
Non-protein N2 components
• Peptides (Flavour)
• Free AA, Histidine (Richer in red muscle than
white)
• Bases like ammonia, trimethylamines
• Creatine, taurine, betaine, histamine, glycine,
uric acid, carnosine, etc.
• Nucleic acids
Fat
• Average fat content between 1-8%
• If fat > 8% fish classified as oily or fat
• Depot fats
• Brain shows highest concentration of fat
• Heart shows lowest
• Fat content determines the quality of fish and
hence its price
• Fatty substances: glyceride group or lipid
group of fatty acids
• Fatty acids: Straight chain monocarboxylic
forms having even no of C atoms (12-28)
• Glyceride group: Fatty acids occur on three
-OH groups of glycerol molecule
Fish Oil
• Marine fish oil contains larger quantities of
C18, C20 and C22
• Fresh water fish oil contains larger quantities
of C16 and C18 but less of C20 and C22
• Large applications in industries
• Shark oil rich in unsaponifiable substances
Minerals
• 1-2% of fish flesh composition
• Concentrated in fish bones
• Principal minerals: Ca, Mg, K, Na, P, S, Fe, Cl,
Cu, Mn, I, Br
• Trace minerals: Sr, Zn, Ba, Al, Pb, Mo, Co, Ni,
Hg, Cd
Vitamins
• Liver rich in B12 and B complex, A and D
• A and D: inner organs of the body
• B: liver, eyes, skin, kidney, spleen and
intestines and roe
• Eels are rich in vitamin A, mackerel in vitamin
D
• Vitamin A, D and E: Fat soluble
• Vitamins of B group: water soluble
Nutritional value of Raw Fish
• Nutritional value of fish meat > meat of cattle
• Less stroma protein in fish
• Excellent source of all essential AA needed in diet
• 300g of fish = ½ of total protein & fat
requirement and 1/4th of calories needed in
balanced daily diet
• This amount also provides 50% P, 30% Fe, 100%
vit A, 30% vit B1 & B2 and 50-100% of niacin req.
Nutritional value of preserved and
processed fish
• Exudation causes 4% loss in proteins, water soluble
vitamins are also lost
• Cooking at 60˚C causes loss in weight due to water loss
• Frying causes heavy loss of essential AA
• Steam/pressure cooking causes loss in proteins and
minerals
• Canning doesn’t affect protein and fat and heat stable
vitamins
• Canning causes considerable losses in vit B1, C,
pantothenic acid & pteroylglutamic acid
• Salting: 1-5% loss in proteins
• Smoking: 8-30% loss in proteins
Freezing & lyophilizing:
• Only vitamin E is destroyed
• Encourage rancidity of fat & denaturation of
proteins
• Undesired look reduces acceptability of the
product, although there is no actual loss of
proteins
FISH BY-PRODUCTS
Fish Protein Concentrate
• FPC is prepared from whole fish or its parts
• Concentration is increased by removal of water,
oil, bones and other materials
• FPC does not bear resemblance to the original
nutritive values of raw fish
• It is a gritty odourless, colourless & tasteless
powder
• Stable at room temperature for 3-4 years
• Components: Highly digestible protein + lysine +
minerals
• Highly nutritious
• For human consumption but not direct
• 5-10% added in bread and biscuits
• 35g/day is recommended level of use of FPC
Fish maws
• Fish maws is the internal gas filled organ that
helps to maintain the buoyancy of fish
• Also called swim bladder/air bladder
• In some Asian cultures fish maws are considered
a food delicacy (China)
• Served in soups or stews
• Used in the food industry as a source of collagen
• Can be made into a strong water resistant glue
• Making isinglass for the clarification of beer
Isinglass
• It’s a collagen derived from thin, inner shiny
silvery layer of airbladder of some fishes
• Airbladders are washed thoroughly, outer thick
fibrous layer is separated from inner layer which
is isinglass raw material
• This raw material is then purified
• Russian isinglass from sturgeons is considered as
a very high quality product
• Good substitute for gelatin in confectionary
• Swells in cold water without dissolving
• At high temp it produces strongly adhesive
gelatin
• Chief use is clarification of beer, vinegar, etc.
• Also used as an adhesive in the form of
cements and court plaster
Fish Body Oil
• Obtained from entire body of fish along with
liver
• Generated from oil sardines / less edible
varieties of fish or from wastes discarded from
canneries
• 5kg of fish = ½ kg fish body oil
• Lesser Vit A and D, less unsaponifiable matter
when compared to liver oil
Uses:
• Source of vit A and D for medicinal purpose
• Manufacture of paints & varnishes
• Leather and tanning industry
• Manufacture of cheap soaps
• Iron and steel industry as a tempering agent
• Manufacture of chemicals, cosmetics and candles
• Fungicides for citrus group of trees
Fish Liver Oil
• Rich source of vit A and D
• Fat of liver is the source of fish liver oil
Uses:
• Prophylactic & curative agent
• High vit A & D enables it to cure rickets, eye
disorders, abnormalities of skin and vertebrae
• Good growth of bone and teeth
• More resistance to counter bacterial attack
Methods of extraction:
• Auto fermentation
• Boiling
• Steaming (85-90˚C under 2kg/sq-cm pressure)
• Chemical digestion
• Solvent extraction
• Refinement of oil
Chitosan
• It is a linear polysaccharide
• Made by treating shrimp and other crustacean shells with
NaOH
• No of commercial and biomedical uses
• Used in agriculture as seed treatment & bio pesticide
• Used in wine making industry, helps to prevent spoilage
• Rapidly clots blood and hence used in bandages and
haemostatic agents
• Research going on for study which shows it limits fat
absorption in the body
• Also used in water filtration
Shark Fin Rays
• Valuable by-product of shark
• Shark fins are dried & soaked overnight in 10%
Acetic acid
• The scale and softened muscles are scraped
off and the rays are separated individually
• Then thoroughly washed and dried
• Essential ingredient in some exotic soups
Fish as food

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Fish as food

  • 1. Fish as food TYBSc Semester VI (Applied component: Marine science) Biren Daftary
  • 2. Biochemical composition of Raw Fish • Principal contents: protein, fat and water • Protein = 20% • Variation in fat and water • Minerals, vitamins and enzymes • No carbohydrate except glycogen in liver • Water = 55 - 83%
  • 3. Proteins • Normal 15 - 20% • In some species < 15% or as high as 28% • Chains of chemical units linked together to make a long molecule • Each unit = amino acid (20) • Essential in human diet for maintenance of good health • Lysine & Methionine are found in high concentrations in fish proteins • Skeletal protein = 50 – 60% of total weight of fish
  • 4. • Protein content classified into following groups: a) Albumin fraction: 16 – 22% b) Protein fraction: 75% c) Stroma: 3%
  • 5. Non-protein N2 components • Peptides (Flavour) • Free AA, Histidine (Richer in red muscle than white) • Bases like ammonia, trimethylamines • Creatine, taurine, betaine, histamine, glycine, uric acid, carnosine, etc. • Nucleic acids
  • 6. Fat • Average fat content between 1-8% • If fat > 8% fish classified as oily or fat • Depot fats • Brain shows highest concentration of fat • Heart shows lowest • Fat content determines the quality of fish and hence its price
  • 7. • Fatty substances: glyceride group or lipid group of fatty acids • Fatty acids: Straight chain monocarboxylic forms having even no of C atoms (12-28) • Glyceride group: Fatty acids occur on three -OH groups of glycerol molecule
  • 8. Fish Oil • Marine fish oil contains larger quantities of C18, C20 and C22 • Fresh water fish oil contains larger quantities of C16 and C18 but less of C20 and C22 • Large applications in industries • Shark oil rich in unsaponifiable substances
  • 9. Minerals • 1-2% of fish flesh composition • Concentrated in fish bones • Principal minerals: Ca, Mg, K, Na, P, S, Fe, Cl, Cu, Mn, I, Br • Trace minerals: Sr, Zn, Ba, Al, Pb, Mo, Co, Ni, Hg, Cd
  • 10. Vitamins • Liver rich in B12 and B complex, A and D • A and D: inner organs of the body • B: liver, eyes, skin, kidney, spleen and intestines and roe • Eels are rich in vitamin A, mackerel in vitamin D • Vitamin A, D and E: Fat soluble • Vitamins of B group: water soluble
  • 11. Nutritional value of Raw Fish • Nutritional value of fish meat > meat of cattle • Less stroma protein in fish • Excellent source of all essential AA needed in diet • 300g of fish = ½ of total protein & fat requirement and 1/4th of calories needed in balanced daily diet • This amount also provides 50% P, 30% Fe, 100% vit A, 30% vit B1 & B2 and 50-100% of niacin req.
  • 12. Nutritional value of preserved and processed fish • Exudation causes 4% loss in proteins, water soluble vitamins are also lost • Cooking at 60˚C causes loss in weight due to water loss • Frying causes heavy loss of essential AA • Steam/pressure cooking causes loss in proteins and minerals • Canning doesn’t affect protein and fat and heat stable vitamins • Canning causes considerable losses in vit B1, C, pantothenic acid & pteroylglutamic acid • Salting: 1-5% loss in proteins • Smoking: 8-30% loss in proteins
  • 13. Freezing & lyophilizing: • Only vitamin E is destroyed • Encourage rancidity of fat & denaturation of proteins • Undesired look reduces acceptability of the product, although there is no actual loss of proteins
  • 15. Fish Protein Concentrate • FPC is prepared from whole fish or its parts • Concentration is increased by removal of water, oil, bones and other materials • FPC does not bear resemblance to the original nutritive values of raw fish • It is a gritty odourless, colourless & tasteless powder • Stable at room temperature for 3-4 years • Components: Highly digestible protein + lysine + minerals
  • 16. • Highly nutritious • For human consumption but not direct • 5-10% added in bread and biscuits • 35g/day is recommended level of use of FPC
  • 17. Fish maws • Fish maws is the internal gas filled organ that helps to maintain the buoyancy of fish • Also called swim bladder/air bladder • In some Asian cultures fish maws are considered a food delicacy (China) • Served in soups or stews • Used in the food industry as a source of collagen • Can be made into a strong water resistant glue • Making isinglass for the clarification of beer
  • 18.
  • 19. Isinglass • It’s a collagen derived from thin, inner shiny silvery layer of airbladder of some fishes • Airbladders are washed thoroughly, outer thick fibrous layer is separated from inner layer which is isinglass raw material • This raw material is then purified • Russian isinglass from sturgeons is considered as a very high quality product • Good substitute for gelatin in confectionary • Swells in cold water without dissolving
  • 20. • At high temp it produces strongly adhesive gelatin • Chief use is clarification of beer, vinegar, etc. • Also used as an adhesive in the form of cements and court plaster
  • 21.
  • 22. Fish Body Oil • Obtained from entire body of fish along with liver • Generated from oil sardines / less edible varieties of fish or from wastes discarded from canneries • 5kg of fish = ½ kg fish body oil • Lesser Vit A and D, less unsaponifiable matter when compared to liver oil
  • 23. Uses: • Source of vit A and D for medicinal purpose • Manufacture of paints & varnishes • Leather and tanning industry • Manufacture of cheap soaps • Iron and steel industry as a tempering agent • Manufacture of chemicals, cosmetics and candles • Fungicides for citrus group of trees
  • 24.
  • 25. Fish Liver Oil • Rich source of vit A and D • Fat of liver is the source of fish liver oil Uses: • Prophylactic & curative agent • High vit A & D enables it to cure rickets, eye disorders, abnormalities of skin and vertebrae • Good growth of bone and teeth • More resistance to counter bacterial attack
  • 26. Methods of extraction: • Auto fermentation • Boiling • Steaming (85-90˚C under 2kg/sq-cm pressure) • Chemical digestion • Solvent extraction • Refinement of oil
  • 27.
  • 28. Chitosan • It is a linear polysaccharide • Made by treating shrimp and other crustacean shells with NaOH • No of commercial and biomedical uses • Used in agriculture as seed treatment & bio pesticide • Used in wine making industry, helps to prevent spoilage • Rapidly clots blood and hence used in bandages and haemostatic agents • Research going on for study which shows it limits fat absorption in the body • Also used in water filtration
  • 29.
  • 30. Shark Fin Rays • Valuable by-product of shark • Shark fins are dried & soaked overnight in 10% Acetic acid • The scale and softened muscles are scraped off and the rays are separated individually • Then thoroughly washed and dried • Essential ingredient in some exotic soups