APBDRF Scientific Advisory Board Meeting December 2013: Research Update

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Ben Decker

Gesundheit & Medizin Lifestyle

2013 Research Update
APBDRF
H. Orhan AKMAN, PhD
Dr. DiMauro’s Laboratory
Columbia University Medical Center
Department of Neurology
New York

Adult Polyglucosan Body Disease
Diagnosis, and Development of tools for Research and Treatment

Mouse model of APBD
Possible Pharmacological
Treatments of APBD
Diagnosis of Manifesting
Heterozygote Patients

Mouse model of APBD with Y329S
mutation
Generation of mouse model of APBD:
Y329S TAT to TCT
Tyr
Ser

FRT
LoxP

5’ ARM 2.1 kb

5’ ARM

Exon 7

Exon 7

PGK- Neomycin

3’ARM 6.5 kb

3’ARM

Breeding scheme and expected
genotypes
Y329S TAT to TCT
Tyr
Ser
FRT
LoxP

5’ ARM 2.1 kb
5’ ARM

1

Exon 7

PGK-Neomycin

3’ARM 6.5 kb
3’ARM

Exon 7

2

1

Expected & other genotypes
Y329S-Neo
5’

Exon 7

PGK-Neomycin

3’

Exon 7

PGK-Neomycin

3’

WT-Neo
5’

Y329S
5’

Exon 7

3’

Exon 7

3’

WT-lox
5’

Exon-7 del or D
5’

3’

Polyglucosan can be used in the liver
during fasting

Difference between Gbe1Y329S and
Gbe1neo/neo mice

Gbe1+/+

Gbe1neo/neo

Percent
change

Percent
change

Gbe1ys/ys

Brain

230 ± 17

25 ± 8

11%

41± 9

n=4

17.8%

Heart

457 ± 56

72 ± 34

16%

92± 21

n=4

20.1%

Liver

855 ± 43

115 ± 42

13%

210± 35

n=4

24.6%

38 ± 18

15%

ND

Muscle 251 ± 23

n=4

ND

Gbe1Y329S mice live longer than
Gbe1neo/neo mice

Gbe1Y329S mice has polyglucosan in the
liver

PAS

Diastase-PAS

Brain
30 days old mice

PAS

Diastase-PAS

Cerebellum
30 days old mice

PAS

Diastase-PAS

Brain 4 month old mice

PAS

Diastase-PAS

Brain 4 month old mice Gbe1ys-neo

PAS

Diastase-PAS

High throughput screening
We have tested 1700 FDA approved
compounds.
Analysis has yield 15 compounds decreasing
PGB content in tissue culture

Control

15

14

13

12

11

10

9

8

7

6

5

4

3

2

1

Relative Glycogen Content
(microgram/mg protein)

Validation of 15 HTS hits

5.0

4.0

3.0

2.0

1.0

0.0

Treatment with Guaiacol

YS untreated

YS treated
PAS

Diastase-PAS

Diagnosis of APBD
GBE enzyme assay
Histologic examination of
sural nerve biopsy
Sequencing genomic DNA
and messenger RNA.

Sequencing mRNA Proof of second
genetic change

Search for the genetic change that
eliminates the normal allele
12 to 15

14 to 16
C P1 P2 P3 C

1 to 6

C P1 P2 P3 C

5 to 10

9 to 14

THANK YOU
Salvatore DiMauro, MD
Sindu Krishna, PhD
Pablo Abreu

Michio Hirano, MD
Valentina Emmanuelle, MD
Carmen Paradas, MD

Sergey Pompou, MD

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