2. Bacteriophages are viruses that infect
bacterial cells.
Infected cells are used as hosts to replicate
the virus.
E. coli phages used due to ease of culture
and quick regeneration
3.
4. It is used for cloning foreign genes with many
other applications
In this process proteins and peptides are fused
to the Capsid(surface) of the phage
The combination of the phage and peptide is
known as a Fusion Protein
5. Different sets of genes are
inserted into the genomes
of multiple phages
These separate phages will
only display one protein,
peptide, or antibody
Collections of these phages
can comprise Libraries
These Libraries are exposed
to selected targets and only
some phages will interact
with targets
6. Recombinant DNA technology to
incorporate foreign cDNA of interest into
viral DNA.
Spliced into gene for a coat protein so
the protein will be displayed on outside
of phage particles
Unbound phages are washed away leaving
only those showing affinity for the
receptors
Eluted phages showing specificity are
used to infect new host cells for
amplification.
Cycle repeated 2-3 times for stepwise
selection of best binding sequence.
7. The analysis process will be done where
phages can be propagated then
characterized with DNA sequencing.
3 types of common phages used in phage
display are the M13 and F1
Virus takes up a small amount of area
Through using multiple Virions polypeptide
libraries can be created, and each phage
displays a random peptide
8.
9. Epitope mapping and mimicking
Identification of new receptors & ligands
Drug discovery
Epitope discovery – new vaccines
Creation of antibody libraries
Organ targeting
10. Proteins Isolated through phage display will
have a specific interactions with its target
Whether this target is a antigen, protein, or
disease it allows a quick effective treatment
for it