Ensimus - a wireless highly selective lactic acid biochemical sensor based on a nanostructured catalyst.

1. by
Wireless highly selective
lactic acid biosensor
based on a nanostructured catalyst

2. About
§ Robo-Med Ltd.
§ Main area of activity:
ü Development of non-invasive biosensors for real-time monitoring of
body’s vital signals;
ü Development of object recognition
methods & 3D reconstruction;
ü Development of rehabilitation and training
systems for the musculoskeletal system.

3. Problem
Untimely or inaccurate diagnosis ->
DISABILITY or DEATH
In Russia only*:
> 1,3 million deaths from cardio-vascular diseases
> 450 000 cases of stroke annually
> 800 000 limb injuries annually
> 1 million cerebral palsy handicaps
Traditional blood analysis for
determining lactic acid doesn’t
work in real-time application
* Data provided by Rosstat, University of Lausanne

4. Solution
Development of a real-time wireless non-invasive
lactic acid biosensor:
§ "Prussian Blue“ is used as the catalyst with polymer immobilization of the
organic enzyme;
§ “Prussian Blue” is nanostructred on the electrode surface, which greatly
improves the biosensor sensitivity and selectivity;
§ Mechatronic, microfluidic design of the sweat collector with micropumps
analysis and wireless data transmission to a smartphone;
§ The biosensor can be used as part of a at-home muscoskeletal
rehabilitation system for control of the critical levels of physical activity.
Competitive Advantages:
Non-invasive Autonomous Ease of use
Personal health management

5. Nanotech component
Reproducibility
Mixing enzymes with
waterproof polymers
Homogeneous
nanopolymeric
membranes
Preparation of the true
polymer solutions
Nanotechnology of exposing enzymes
to organic solvents

6. Nanotech component
Biosensor structure:
1. An electrode consisting of layers
of silver, graphite and insulator is
coated with a layer of Prussian Blue
using the nanostructuring method.
2. The Prussian Blue layer is
covered with a thin layer of true
polymer solution with enzyme
molecules and as a result we get a
homegenous nanopolymer
membrane which is less than 100
nm thick.
3. Enzyme molecule diameter is
about 7 nm, substrate molecules -
0,8 nm.
Electrode
Nanopolymer membrane
Lactic
acid
Piruvate
Lactate oxidase
Lactate oxidase
Prussian Blue

7. Product characteristics
§ Lac tate, cardio, temperature non-invasive sensors
§ Real-time flow system (application: sports, rehab)
§ Monitoring service (cloud subscription)
§ Control of vital signals & critical physical loads, history
§ Teleconsultations and social networks
§ Rehabilitation & training system
§ Flow sensor + subscription
§ 3D reconstruction system with
biomechanics real-time analysis
§ Rehab treadmill system (optional)

8. Rehab system (sensor + AKS
+ subscription)
Subscription
Analysis of kinematic signatures (AKS)

9. Market Structure
9
MHealth
$1.5
billion
(2012)
$21.5
billion
(2018)
Ensimus
(No-­‐risk
PHM*)
Rehab
market
$29.6
billion
(2014)
$38.8
billion
(2018)
Mo:on
Capture
$4.8
billion
(2016)
Home
telemedicine
$10
billion
(2019)
$611
million
(2014)
$4548
million
(2018)
*Persnal Health Management
Data sources: RBC, РАТМ, ACRM (American Congress of Rehab Medicine)

10. Target markets
10
Home use:
§ Injury and heart stroke rehab;
§ Physical load control, monitoring progress
for athletes and fitness enthusiasts.
Professional use:
§ High performance sports
§ Transplant surgery
§ Newborn screening for genetic disorder
- cystic fibrosis

11. Marketing & Sales
Main market Secondary market
Cer:fied
suppliers,
integrators,
tenders
and
auc:ons
Commercial
fitness
equipment
resellers,
sport
medicine
and
rehab
centers/
clinics
Stratgey
Distribu:on
channels
B2G:
Working
with
departmental
health
organiza:ons
B2В:
Par:cipa:on
in
expos,
trade
fairs,
associa:ons,
communi:es
B2С:
social
marke:ng,
populariza:on,
par:cipa:on
and
crea:on
of
health
context
based
communi:es
E-­‐shops.
Direct
sales
network
Partner
network

12. Project status & break-even
12
2014
Demo
version

13. Partners
16
Pre-Seed
Investment
Fund
North-West Center
for Technology
Transfer
Saint-Petersburg
Administration

14. Contact us
Anton Panfilov
Phone: +7(926) 925-3763
Skype: AVP1962
Email: panfilov@tradition.ru
http://www.robo-med.ru
17