090130_Journal_Club

Photoactivated Transcription 1/29/09 Andrew Hires Svoboda Journal Club

Photoactivated Transcription in Neurospora (Payen 1843) ,[object Object],[object Object]

Species Dependent Strategies ,[object Object],[object Object],[object Object],[object Object],[object Object]

Some Examples ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

phyR : Peak induction within 1 hour

[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

phyR : Performance ,[object Object],50:1 LacZ induction, 1/5 expression of wild-type GAL4 system

phyR : Photon Dosing ,[object Object],1 minute pulse best 1mmol 660nm photons = 180 J 180 J / s m 2 = 180W / m 2 for 1s, or 3W / m 2 for 1 minute Filtered fluorescents for long term LEDs for pulsed activation

phyR : Temporal precision and reversibility ,[object Object],[object Object]

phyR : Issues ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

WC : Light activated transcription factor ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

WC : Light Responsive Element sequence

WC : wc-1 gene structure ,[object Object],[object Object],[object Object],[object Object],[object Object]

WC : FAD required for light sensitivity ,[object Object],[object Object],[object Object]

WC : Photon Dose and Wavelength ,[object Object],[object Object],[object Object]

WC : Two-photon action spectra ,[object Object],Heikal & Webb 2002

WC : Issues ,[object Object],[object Object],[object Object],[object Object],[object Object]

090130_Journal_Club

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