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Diagrams of different types of protozoa
                Amoeba
         Shelled amoeba

             Flagellates

          Ciliates – suctoria

         Ciliates – peritrichus

            Heliozoanes
   Free living vs. symbiosis
   Photosynthetic(autotrophy)
   Combinations(hetrotrophy)
   Absorb solutes(osmotrophy)
   Ingest particulates(phagotrophy)-
    predation on bacteria and other protozoa.
   Pinocytosis (fluid uptake)
   Factors affecting distribution of free living forms
   Nacl concentration,
   pH,
   Temperature,
   Oxygen requirements.
  Nutrition
--free living forms – phagocytosis of particulate
   matter.
--Complex nutritional needs.
--All are chemoheterotrops .
--Storage products –glycogen , starch and lipids.
• One organism lives on other organism ,
                 called the host.eg.-cuckoo(koel) and the
 Parasitism      crow.
               • The host is harmed, but usually survives.



               • One organism benefits and the other neither
Commensalism     benefits nor is harmed.
               • Example-Orchid growing on a mango tree.



               • Both organisms are benefited from the
  Mutualism      relationship.
               • Example-association of fungus and algae.
   Osmotrophy is the uptake of dissolved organic
    compounds by osmosis for nutrition
   Pinocytosis, a process by which liquid droplets are
    ingested by living cells. It is a process by which
    cells engulf external substances, gathering them
    into special membrane-bound vesicles contained
    within the cell. In pinocytosis, rather than an
    individual droplet of liquid traveling passively
    through the cell membrane, the droplet first
    becomes bound, or adsorbed, on the cell
    membrane, which then invaginates (forms a
    pocket) and pinches off to form a vesicle in the
    cytoplasm.
Asexual
1.     Fission
     - characterized by division of the body into two or more parts, each of
       which develops into a complete individual.
2.     Budding
     - self supportive cell sprouts from & detaches from the parent
       organism.
3.     Schizogony
      - a multiple fission type; the nucleus undergoes multiple divisions
       before the cell divides.
Sexual
1. Conjugation
    - the simplest form of reproduction. Two single-celled organisms link
      together, exchange genetic information, & then separate.



                                               - Reproduction -
   Conjugation in Protozoa is a two-way process,
    genetic material is passed between each
    conjugant.
   The process of union of two gametes to form a
    zygote. It involves both plasmogamy and
    karyogamy.
   The macroconjugants resemble the normal
    feeding individuals.
    The microconjugants resemble the swarmers,
    although smaller.
Conjugation
The life stages of protozoa are:-
         – latent survival form.
                  - The active motile stage of
  protozoan parasite or active pathogenic
  vegetative state.
- Some protozoa are
  coated with a
  protective capsule
  called cyst.


Cyst permits organism
  to survive when food
  & oxygen are lacking
  or when temperature
  is not suitable &
  toxic chemicals are
  present.
-   Cyst is a reproductive structure in which
    new cells are produced asexually.

- The breaking of cyst is called excystation.
Tropozoite
s
Entamoeba
               histolytica is
               the causative
               agent of
               amoebic
               dysentery.
It is the
only
photogenic
amoeba
found in the
animal
African

   Trypanosomiasis
It is named as
sleeping disease
that is caused by
tyrpanosoma brucei
gambience &
trypanosoma brucei
rhodesiense-flagellates
that are injected
by tsetse fly.
   Do all mosquito bite human beings ?

   Both males and females obtain some nutrition
    from flower nectar, but it is only the females
    that feed on blood to acquire the extra protein
    boost needed to produce and lay eggs.
    Male mosquitoes do not bite human beings.
    They live on plant juices, other natural liquids
    from plants and decomposing organic material.
1. Malaria
- A protozoan
  cardiovascular
  disease caused by
  anopheles mosquito
  through injection
  of protozoan
  parasite
  plasmodium.
 Plasmodium malariae,
 Plasmodium ovale,

 Plasmodium vivax,

 Plasmodium flaciparum.
Plasmodium
     life
     cycle

-   Anopheles mosquito
    feeds on blood &
    serves as the
    vector.

- Sporozoites enter
  blood stream and
  proceed to the
  liver within 30 min
  to infect
- 1 week = Multiplication &
   transformation in the liver leads
   to the release of about 5,000-
   10,000 merozites PER
   HEPATOCYTE infected with only
   one sporozite!

-   Merozites infect red blood cells,
    initiating the symptoms and
    pathology of malaria.

-   Merozites replicate and
    differentiate again

-   Cell ruptures to release new
    merozites (which will infect more
    red blood cells)

-   Some merozites differentiate into
     ♂ and ♀ gametocytes.

-   Gametocytes ingested by Female
    Anopheles differentiate into
    male and female gametes which
    fuse to form a zygote and
    become sporozites in the salivary
    gland….
-   Chills, fever, sweating—
    peak every 48hrs.


-   Weak, anemic,
    splenomegaly.


- Blocked capillaries =
  intense headaches,
  renal failure, heart
  failure, or cerebral
  damage.
Diagnosis
- The most common diagnostic test is the blood
   smear.


Treatment
- Drug quininine, chloroquine, primaquine, &
   mefloquine.
Disease              Casual agents       Organs affected         vector

1. Amoebiasis        Entamoeba           Intestine, Liver        Water
                     histolytica


2.African Sleeping   Trypanosoma brucei Blood, Brain             Tsetse fly (
Sickness                                                         Glossina)


3.Leishmaniasis (    Leishmania          White Blood             Sand fly (
Kala - azar)         donovani            Cells,Skin, Intestine   Phlemotomus)


4.Malaria            Plasmodium spp.     Liver, Red Blood        Mosquito (
                                         Cells                   anopheles)


5. Babesiosis        Babesia microti     Red Blood Cells         Tick ( Txode)
Protozoa play important roles in the fertility
of soils. By grazing on soil bacteria, they
regulate bacterial populations and maintain
them in a state of physiological youth in the
active growing phase. This enhances the
rates at which bacteria decompose dead
organic matter. Protozoa also excrete
nitrogen and phosphorus, in the form of
ammonium and orthophosphate, as products
of their metabolism, and studies have shown
that the presence of protozoa in soils
enhances plant growth.
Protozoa play important roles in
wastewater treatment .

Good-quality, clean effluents are
produced in the presence of large ciliated
protozoan communities because they
graze voraciously on dispersed bacteria
and because they have the ability to
flocculate suspended particulate matter
and bacteria.
                        Importance
Protozoa probably play a similar role in
 polluted natural ecosystems. Indeed, there
 is evidence that, by feeding on oil-degrading
 bacteria, they decrease bacterial growth in
 much the same way they enhance rates of
 decomposition in soils, thereby speeding up
 the breakdown of oil spillages.




                           Importance
Protozoa
Protozoa

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Protozoa

  • 1. Diagrams of different types of protozoa  Amoeba  Shelled amoeba  Flagellates  Ciliates – suctoria  Ciliates – peritrichus  Heliozoanes
  • 2. Free living vs. symbiosis  Photosynthetic(autotrophy)  Combinations(hetrotrophy)  Absorb solutes(osmotrophy)  Ingest particulates(phagotrophy)- predation on bacteria and other protozoa.  Pinocytosis (fluid uptake)
  • 3. Factors affecting distribution of free living forms  Nacl concentration,  pH,  Temperature,  Oxygen requirements.
  • 4.  Nutrition --free living forms – phagocytosis of particulate matter. --Complex nutritional needs. --All are chemoheterotrops . --Storage products –glycogen , starch and lipids.
  • 5. • One organism lives on other organism , called the host.eg.-cuckoo(koel) and the Parasitism crow. • The host is harmed, but usually survives. • One organism benefits and the other neither Commensalism benefits nor is harmed. • Example-Orchid growing on a mango tree. • Both organisms are benefited from the Mutualism relationship. • Example-association of fungus and algae.
  • 6. Osmotrophy is the uptake of dissolved organic compounds by osmosis for nutrition  Pinocytosis, a process by which liquid droplets are ingested by living cells. It is a process by which cells engulf external substances, gathering them into special membrane-bound vesicles contained within the cell. In pinocytosis, rather than an individual droplet of liquid traveling passively through the cell membrane, the droplet first becomes bound, or adsorbed, on the cell membrane, which then invaginates (forms a pocket) and pinches off to form a vesicle in the cytoplasm.
  • 7. Asexual 1. Fission - characterized by division of the body into two or more parts, each of which develops into a complete individual. 2. Budding - self supportive cell sprouts from & detaches from the parent organism. 3. Schizogony - a multiple fission type; the nucleus undergoes multiple divisions before the cell divides. Sexual 1. Conjugation - the simplest form of reproduction. Two single-celled organisms link together, exchange genetic information, & then separate. - Reproduction -
  • 8.
  • 9.
  • 10. Conjugation in Protozoa is a two-way process, genetic material is passed between each conjugant.  The process of union of two gametes to form a zygote. It involves both plasmogamy and karyogamy.  The macroconjugants resemble the normal feeding individuals.  The microconjugants resemble the swarmers, although smaller.
  • 12. The life stages of protozoa are:- – latent survival form. - The active motile stage of protozoan parasite or active pathogenic vegetative state.
  • 13. - Some protozoa are coated with a protective capsule called cyst. Cyst permits organism to survive when food & oxygen are lacking or when temperature is not suitable & toxic chemicals are present.
  • 14. - Cyst is a reproductive structure in which new cells are produced asexually. - The breaking of cyst is called excystation.
  • 16. Entamoeba histolytica is the causative agent of amoebic dysentery. It is the only photogenic amoeba found in the animal
  • 17. African Trypanosomiasis It is named as sleeping disease that is caused by tyrpanosoma brucei gambience & trypanosoma brucei rhodesiense-flagellates that are injected by tsetse fly.
  • 18. Do all mosquito bite human beings ?  Both males and females obtain some nutrition from flower nectar, but it is only the females that feed on blood to acquire the extra protein boost needed to produce and lay eggs.  Male mosquitoes do not bite human beings. They live on plant juices, other natural liquids from plants and decomposing organic material.
  • 19. 1. Malaria - A protozoan cardiovascular disease caused by anopheles mosquito through injection of protozoan parasite plasmodium.
  • 20.  Plasmodium malariae,  Plasmodium ovale,  Plasmodium vivax,  Plasmodium flaciparum.
  • 21. Plasmodium life cycle - Anopheles mosquito feeds on blood & serves as the vector. - Sporozoites enter blood stream and proceed to the liver within 30 min to infect
  • 22. - 1 week = Multiplication & transformation in the liver leads to the release of about 5,000- 10,000 merozites PER HEPATOCYTE infected with only one sporozite! - Merozites infect red blood cells, initiating the symptoms and pathology of malaria. - Merozites replicate and differentiate again - Cell ruptures to release new merozites (which will infect more red blood cells) - Some merozites differentiate into ♂ and ♀ gametocytes. - Gametocytes ingested by Female Anopheles differentiate into male and female gametes which fuse to form a zygote and become sporozites in the salivary gland….
  • 23. - Chills, fever, sweating— peak every 48hrs. - Weak, anemic, splenomegaly. - Blocked capillaries = intense headaches, renal failure, heart failure, or cerebral damage.
  • 24. Diagnosis - The most common diagnostic test is the blood smear. Treatment - Drug quininine, chloroquine, primaquine, & mefloquine.
  • 25. Disease Casual agents Organs affected vector 1. Amoebiasis Entamoeba Intestine, Liver Water histolytica 2.African Sleeping Trypanosoma brucei Blood, Brain Tsetse fly ( Sickness Glossina) 3.Leishmaniasis ( Leishmania White Blood Sand fly ( Kala - azar) donovani Cells,Skin, Intestine Phlemotomus) 4.Malaria Plasmodium spp. Liver, Red Blood Mosquito ( Cells anopheles) 5. Babesiosis Babesia microti Red Blood Cells Tick ( Txode)
  • 26. Protozoa play important roles in the fertility of soils. By grazing on soil bacteria, they regulate bacterial populations and maintain them in a state of physiological youth in the active growing phase. This enhances the rates at which bacteria decompose dead organic matter. Protozoa also excrete nitrogen and phosphorus, in the form of ammonium and orthophosphate, as products of their metabolism, and studies have shown that the presence of protozoa in soils enhances plant growth.
  • 27. Protozoa play important roles in wastewater treatment . Good-quality, clean effluents are produced in the presence of large ciliated protozoan communities because they graze voraciously on dispersed bacteria and because they have the ability to flocculate suspended particulate matter and bacteria. Importance
  • 28. Protozoa probably play a similar role in polluted natural ecosystems. Indeed, there is evidence that, by feeding on oil-degrading bacteria, they decrease bacterial growth in much the same way they enhance rates of decomposition in soils, thereby speeding up the breakdown of oil spillages. Importance

Hinweis der Redaktion

  1. Free living don’t require host.
  2. Free living means don’t require host cell.
  3. Symbiosis is the close relationship between two organisms.
  4. Plasmodium flaciparum is the most severe and prevalent.
  5. Splenomegaly is an enlargement of the spleen.