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“ Cataloging Computationally Putative Resistance Genes in Grape and Their Corresponding Pathogenicity Genes” Bioinformatics Project Dr. Yaqoub Ashhab Amer Wazwaz & Murad Shneawer  26/5/2011
Outline ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Introduction ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Project’s Procedure ,[object Object],[object Object],[object Object],[object Object],[object Object]
Real Examples (1) rpfF   Xylella fastidiosa  subsp  Temecula1 Gene ID :  1144610   Xylella fastidiosa  subsp .  fastidiosa  complete genome  ACCESSION CP002165
Xylella fastidiosa
Real Examples (2) virB2   Agrobacterium tumefaciens Gene ID :  1224319   Agrobacterium vitis complete sequence  NC_011988.1  :  Accession
Agrobacterium vitis
Agrobacterium vitis
[object Object],[object Object],2. Resistance Genes
[object Object],[object Object],[object Object],[object Object],WHY Arabidopsis Thaliana as a model   plant
Search for Resistance Genes in Arabidopsis Thaliana
1. PR5 ( Gene ID:   843842)  CDS
PR5 v.s Vitis vinifera
 
82% identity in Vitis vinifera
Vitis vinifera   PR5 v.s CDS identity 82%
 
2. NPR1 ( Gene ID: 842733)  CDS
Blast NPR1 v.s Vitis vinifera
NPR1 V.S 72 % Vitis vinifera
3. Blast RPS4  Gene ID ( 834561) CDS V.S Vitis vinifera
83%Vitis vinifera   RPS4 V.S
 

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Cataloging computationally putative resistance genes in grape and