Seminario de Biologia Molecular, Tercer semestre Medicina Universidad Pontificia Bolivariana.

1. HAFF
DISEASE
1
Maria Alejandra Escobar Salamanca
III semestre

2. Introduction
A member of the aldo-keto
reductase (AKR)
superfamily, c in L-ascorbic
biosynthesis
2
AKR1A Vit C Kefir
Is an essential nutrient for
in vitro and in vivo
osteoblast differentiation.
Is an alcoholic and acidic
fermented beverage.

3. Introduction
3

4. General objective
4
“Evaluate the osteoprotective effects of kefir peptides
(KPs) in AKR1A1eGFP/eGFP mice and uncover the
underlying mechanism of KPs in the modulation of
bone remodeling”

5. 01 Mice 02
● Wild type (WT)
● AKR1A1 eGFP/eGFP transgenic mice
(CD-1) were generated in our laboratory by
pronuclear microinjection,locus of AKR1A1
gene. On mouse chromosome 4 was
integrated with the pCAG-eGFP transgene.
Serum assays
● ELISA
● Proinflammatory cytokines
● Serum biomarkers of bone remodeling
Materials and methods
5

6. Materials and methods
6
Western blot
● Be separated by electrophoresis and
later they must be transferred to a
membrane
03
Preparation of BMMs and
BMMSCs test
04
● The bone marrow was harvested by cutting the
ends of the femurs and tibias
● BMM (, bone marrow macrophage) ->
osteoclast differentiation
● BMMSC, bone marrow mesenchymal stem cell
-> osteoblast differentiation
● Alkaline phosphatase

7. Results 7

8. 8
Results

9. Discussion
9
Authors
] S.X. Zheng,Y.
Vrindts, M. Lopez,
D. De Groote, P.F.
Zangerle, J.
Collette, et al
Significant difference in IL-1β, IL-6 and TNF-α production between
osteoporotic and control subjects, with higher serum levels of these
cytokines in osteoporotic women
+
M. Martiniakova,
M. Babikova, R.
Omelka
With antioxidative or anti-inflammatory functions have been
demonstrated to ameliorate osteoporosis
+
P. Ducy, M.
Starbuck, M.
Priemel, J. Shen,
G. Pinero, V.
Geoffroy, et al.,
Runx2 is required for the synthesis of bone matrix by mature
osteoblasts
+

10. 1
In order to treat complex diseases
such as osteoporosis, which
deteriorate the quality of life of many
people around the world, we must
have a cytological vision of what is
happening, to understand the signs
and symptoms of the disease itself,
understand it from the micro to the
macro
2
Today we not only need
diagnostic tests that give us
the name of the disease, but
also that explain its origin.
Conclusions
10

11. 11