thalidomide tragedy immunosuppressant immunostimulant immunomodulator types of immunity immune system regulatory framework in india drug development phases phases of clinical trials gcp ich guidelines counting of viable cells application of cell culture characterization of cells cryoprotectant cryopreservation coulter counter method cell counting technique haemocytometer main test sighting study inhalational chamber sub-chronic toxicity subacute toxicity acute toxicity oecd guidelines inhalational toxicity studies toxicity studies
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