troduction History Types of array   Protein arrays in situ Protein in-situ arrays PISA NAPPA DAPA   Application  Conclusion References

1. PROTEIN ARRAY
By
KAUSHAL KUMAR SAHU
Assistant Professor (Ad Hoc)
Department of Biotechnology
Govt. Digvijay Autonomous P. G. College
Raj-Nandgaon ( C. G. )

2. Contents of the topic :
Introduction
History
Types of array
Protein arrays in situ
Protein in-situ arrays
PISA
NAPPA
DAPA
Application
Conclusion
References

3. Introduction:

4. History:
Microarray ELISA-style assays will accelerate immunodiagnostics
significantly.
This aspect of the technology was discussed in the 1980s by Ekins,
who Introduced the concept of the ambient analyte assay and
demonstrated that microspot immunoassays could be perfomed
with high sensitivity and selectivity.

5. DEFINITION

6. Types of protein
arrays:

9. Various types of protein array:
Ab array

10. Various types of protein array:
Ab array

11. Cell/Tissue array

12. Surface plasmone array

13. Protein array formed by concatamer structure:

15. Protein arrays In situ techniques:
PISA method :

16. Protein arrays In situ techniques:
NAPPA method:

17. Protein arrays In situ techniques:
DAPA method :

18. Protein arrays In situ techniques:
DAPA method :

19. Protein arrays In situ techniques:
Screening for protein functions and interactions:
Remaining Challenges :
Capture reagents and cross-reactivity:

20. Application:

21. Principle of Gene manipulation & Genomics :by
Primrose & twymane:2nd edition ,2004
Proteomics by Timothy Palzkill,2001 ,1st edition
References