2. What is CBC?
Complete Blood Count :
Hb, RBC, MCV, MCH, MCHC, WBC & Diff, Platelet,
Reticulocyte
3. Anemia
• RBC mass
• ed level of Hb more than 2SD of mean normal of Hb
according to age
Age Hb level
• New born <13 gr/dl
• 2-3 months < 9 FT
< 7 premature
• 6m-2y <9.5
• 2y – 6 years old <10.5
• 6 – 12 y/o <11.5
• >12 y/o Male < 14
Female < 12
4. MCV
• Mean corpuscular volume: 100 (fl)
• Age: 2-10 y/o MCV= Age (year) + 70
• Age ≥ 10 y/o MCV < 80: Microcytosis
MCH
• Mean corpuscular hemoglobin: 100 (Pg)
• More sensitive than MCV
• MCH 25- 27 hypochromia
Rbc
HCT
5. MCHC
• Mean corpuscular hemoglobin concentration:
100 100
• It is important in diagnosis of congenital
Spherocytosis (MCHC > 35)
:
Rbc
Hb
Rbc
HCT
7. The Three Basic
Measures
Measurement Normal Range
A. RBC count 5 million 4 to 6
B. Hemoglobin 15 g% 12 to 17
C. Hematocrit 45 38 to 50
A x 3 = B x 3 = C - This is the rule of thumb
Check whether this holds good in given results
If not -indicates micro or macrocytosis or hypochro.
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8. Anaemia – First Test
RETICULOCYTE COUNT %
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Normal
Less than 2%
• ‘RBC to be’ or Apprentice RBC
• Fragments of nuclear material
• RNA strands which stain blue
9.
10.
11. The reticulocyte count
(kinetic approach)
• Increased reticulocytes (greater than 2-3% or
100,000/mm3 total) are seen in blood loss and
hemolytic processes, although up to 25% of
hemolytic anemias will present with a normal
reticulocyte count due to immune destruction of red
cell precursors.
• Retic counts are most helpful if extremely low
(<0.1%) or greater than 3% (100,000/mm3 total).
12. The reticulocyte count
• To be useful the reticulocyte count must be adjusted for
the patient's hematocrit. Also when the hematocrit is
lower reticulocytes are released earlier from the marrow
so one can adjust for this phenomenon. Thus:
• Corrected retic. = Patients retic. x (Patients Hct/45)
• Reticulocyte index (RPI) = corrected retic.
count/Maturation time
(Maturation time = 1 for Hct=45%, 1.5 for 35%, 2 for
25%, and 2.5 for 15%.)
• Absolute reticulocyte count = retic x RBC number.
15. Workup – Second Test
• The next step is ‘What is the size of RBC’ ?
• MCV indicates the Red cell volume (size)
• Both the MCH & MCHC tell Hb content of RBC
• If the RPI is 2 or less
• We are dealing with either
• Hypoproliferative anaemia (lack of raw material)
• Maturation defect with less production
• Bone marrow suppression (primary/ secondary)
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18. CLASSIFICATION
• Classification by Pathophysiology
• Blood Loss
• Decreased Production
• Increased Destruction
• Classification by Morphology
• Normocytic
• Microcytic
• Macrocytic
19. Anaemia Workup – 3rd Test
Red cell Distribution Width – RDW
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RDW < 13
Mean 90 fl
RDW is 13
MCV 90 fl
20. RDW
• Red cell distribution width = anisocytosis
• RDW = 11-14.5%
• IDA: RDW
• -thalassemia minor: NL or RDW
21. Anaemia Workup - 4th Test
Peripheral Smear Study
• Are all RBC of the same size ?
• Are all RBC of the same normal discoid shape ?
• How is the colour (Hb content) saturation ?
• Are all the RBC of same colour/ multi coloured ?
• Are there any RBC inclusions ?
• Are intra RBC there any hemo-parasites ?
• Are leucocytes normal in number and D.C ?
• Is platelet distribution adequate ?
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23. RBC morphology
• 7-9 m with 1/3 central palor
• Lifespan of 110-120 days
• About the size of nucleus of normal lymphocyte
• Poikilocytosis & Anisocytosis
26. Howell Jolly body
• Nuclear remnant - DNA
• hemolytic anemia
• absent or hypofunction
spleen
27. HISTORY
AGE: Nutritional iron deficiency is never responsible for
anemia in term infants before 6 months of age; rarely seen in
premature infants before the time that they have doubled their
birth weight.
Anemia occurring in the neonatal period is generally the result
of recent blood loss, isoimmunization, or initial manifestation of
a congenital hemolytic anemia or congenital infection
Anemia first detected at 3 to 6 months of age suggests a
congenital disorder of hemoglobin synthesis or hemoglobin
structure
Gender: Consider X-linked disorders in males (G6PD
deficiency, pyruvate kinase deficiency)
28. History
Drug: Oxidant-induced hemolytic anemia, phenytoin (Dilantin)-
induced megaloblastic anemia, drug-induced aplastic anemia
Infectious: Hepatitis-induced aplastic anemia, infection-induced
red cell aplasia, hemolytic anemia
Inheritance: Family history of anemia, jaundice, gallstones, or
splenomegaly
Diarrehia: Suspect small bowel disease with malabsorption of
folate or vitamin BIz. Suspect inflammatory bowel disease with
blood loss. Suspect exudative enteropathy with blood loss
29. History
Neonatal: A history of hyperbilirubinemia in the
newborn period suggests the presence of congenital
hemolytic anemia, such as the hereditary spherocytosis
of G6PD deficiency. Prematurity predisposes to the
early development of iron deficiency
Diet:Document sources of iron, vitamin BIz, folic
acid, or vitamin E in the diet. A history of pica,
geophagia, or pagophagia suggests the presence of iron
deficiency
48. Microcytic hypochromic
anemia
1. IDA
2. or thalassemia trait
3. Sideroblastic anemia
4. Anemia of chronic disease
5. Lead poisoning
6. Copper deficiency
7. Malnutrition
49. DDX of Microcytosis
Iron deficiency anemia Familial telangiectasia (OWR)
idiopathic pulmonary
hemosiderosis, PNH
- thalassemia No simple test, trial of iron therapy
- thalassemia Thal minor - elevated Hb A2 if not
iron deficient
Anemia of chronic disease MCV 75-82; inadequate
erythropoietin response
Hb CC African-American, splenomegaly,
mild anemia MCV 65, many target
cells
Hb EE Southeast Asian, no anemia or
minimal anemia. MCV 65 many
target cells
50. Approach to Dx
• Hx- age,sex,pica,infection, duration, onset, subjacent
illness, blood loss (GI, menstruation, surgery…), diet,
medications, toxic exposure, occupation, Family Hx,
Social Hx
• PE- complete exam including skin (jaundice, petechiae),
HEENT, Abdomen (hepatosplenomegally), lymphatics,
rectal, and pelvic
71. Lab Test for DDx of Common Microcytic Hypochromic Anemias
Iron
Deficiency
Thalassemia
Minor
Chronic
Disease
Scrum Iron N or
TIBC N or N or
% Iron
Saturation
(< 10%) N or
Serum Feritin
FEP N
72. Hemolytic anemias
•Coomb’s test direct negative, indirect positive = delayed
transfusion reaction
•Hereditary spherocytosis
•Osmotic fragility test: fresh and incubated
•Autosomal dominant, spectrin decreased, splenectomy
•Sickle cells
•Sickledex- solubility screening test for hgb S
•Cellulose acetate electrophoresis necessary to define AS, SS, SC, S-
thal etc
•Elliptocytes
•Most are not anemic or have compensated hemolytic process
•10-15% have chronic hemolytic anemia
80. RBC morphology
•7-9 m with 1/3 central palor
•Lifespan of 110-120 days
•About the size of nucleus of normal lymphocyte
•Poikilocytosis & Anisocytosis
97. Coombs’ Negative Hemolytic Anemia
Membrane Defects
Spherocytosis
•Common among Northern
European
•Autosomal dominant
•Decreased spectrin
•Osmotic fragility test
•Autohemolysis test
Elliptocytosis
•90% with no clinically
significant hemolysis
•Abnormal membrane
protein
98. Coombs’ Negative Hemolytic Anemia
Deficiency of RBC Enzymes
Pyruvate Kinase Def.
•Severe anemia in
newborns
•Adults symptomatic
•Jaundice
•Splenomegaly
•Fluorescent screening test
•Quantitative test
G6PD Def.
•X-linked
•Mediterranean, African
American, and Asian
•Oxidant drugs – ASA, quinine,
primaquine, chloroquine,
sulfacetamide, sulfamethoxazole,
nitrofurantoin, chloramphenicol,
procainamide, quinidine
•Infections
•Quantitative test
99. Coombs’ Negative Hemolytic Anemia
Hemoglobinopathy
HbS disease
•Valine substitution for
Glutamic acid at the 6th
position of b-chain
•Sickle crises
•Severe anemia
•Screening test - Na
Metabisulfite solubility
•Hgb electrophoresis
105. Hemolytic Anaemia
Anemia of increased RBC destruction
– Normochromic, normocytic anemia
– Shortened RBC survival
– Reticulocytosis – due to ↑ RBC destruction
Will not be symptomatic until the RBC life span is
reduced to 20 days – BM compensates 6 times
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106. Tests Used to Diagnose Hemolysis
.1Reticulocyte count
.2Combined with serial Hb
.3Serum LDH
.4Serum bilirubin
.5Haptoglobin
.6Urine hemosiderin
.7Hemoglobinuria
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