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An Introduction to
Synthetic Biology
“Hacking DNA for Fun and Profit”
1. what?
2. applications
3. the tech
4. free and safe
5. go hack
1. what?
2. applications
3. the tech
4. free and safe
5. go hack
Engineering
 vs Science
Understanding,
             Models



Science                  Engineering



             Artifacts
              (cells)
Synthetic Biology
Synthetic Biology
         =
GeneticEngineering++
1. Recombinant DNA
2. PCR
3. Automated sequencing
1. Recombinant DNA
2. PCR
3. Automated sequencing
4. Automated construction
5. Standardization
6. Abstraction
Specifications


Testing/Validation                           Design


                      Standard
                     Engineering

 Implementation
                       Practice             Modelling




        The Imperial College of London, iGEM 2006
Specifications        Design           Modelling        Implementation       Testing
Start!
         Specifications
           oscillator
                              Based on
                            Lotka-Volterra
                                               Modelling
                                              Lotka-Volterra
                             Based on
                           Quorum Sensing
                                                Modelling
                                               Full system
          Specifications
         test constructs
                               Design
                           test constructs
                                                Modelling
                                              Test construct
                                                                      Build
                                                                 test constructs
                                                                                    Characterized
                                                                                   test constructs
                                                Modelling
                                             characterized sys
                                                                    Build full
                                                                     system
                                                                                   Characterized
                                                                                    Full system      Our Goal!




               The Imperial College of London, iGEM 2006
1. what?
2. applications
3. the tech
4. free and safe
5. go hack
Biofuels
Bioremediation
Medicine
Space ISRU
1. what?
2. applications
3. the tech
4. free and safe
5. go hack
$1/bp, 4wk
e. coli: 4k
you and me: 3gbp
Standardzied
  Assembly
Common
Signal Carrier
Polymerase
Operations
per Second
Characterization
Composition
& Abstraction
http://www.ccbi.cam.ac.uk/iGEM2006/index.php/Description
http://www.ccbi.cam.ac.uk/iGEM2006/index.php/Description
1. what?
2. applications
3. the tech
4. free and safe
5. go hack
Legal Frameworks
Human Factors
1. what?
2. applications
3. the tech
4. free and safe
5. go hack
http://www.igem.org
http://parts.mit.edu
http://www.openwetware.org
Questions!
diybio.org
    igem.org
  parts.mit.edu
openwetware.org
 biohack.sf.net

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Synthetic Biology at BarcampRochester3