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ISOLATION AND CHARACTERISATION OF HUMAN FETAL LIVER STEM CELLS WITH AFP, CK 18 & ALBUMIN PRESENTED BY S.EZHIL MANGAI

UNDER THE GUIDANCE OF ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

INTRODUCTION ,[object Object],[object Object],[object Object],[object Object]

PROPERTIES OF STEM CELLS ,[object Object],[object Object]

TYPES OF STEM CELLS ,[object Object],[object Object],[object Object],[object Object],[object Object]

OBJECTIVE ,[object Object],[object Object]

STEPS INVOLVED ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

CONTINUE… ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

LIVER CELL EXTRACTION ,[object Object],[object Object]

ISOLATION OF STEM CELLS ,[object Object],[object Object],[object Object]

MAGNETIC ASSOCIATED CELL SORTER ,[object Object],[object Object],[object Object]

CONTINUE… ,[object Object],[object Object],[object Object]

CONTINUE… ,[object Object],[object Object],[object Object],[object Object]

CELL COUNTING ,[object Object],[object Object],[object Object],[object Object]

RESULTS ,[object Object],[object Object],[object Object],[object Object],[object Object]

APPLICATIONS ,[object Object],[object Object],[object Object],[object Object]

OBJECTIVE ,[object Object],[object Object],[object Object]

VIABILITY ASSAY ,[object Object],[object Object],[object Object]

IMMUNOCYTOCHEMISTRY ,[object Object],[object Object],[object Object],[object Object]

PROCEDURE ,[object Object],[object Object],[object Object]

[object Object],[object Object],[object Object],[object Object],[object Object]

MTT ASSAY ,[object Object],[object Object]

[object Object],[object Object]

PROCEDURE ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

RESULTS ,[object Object],[object Object],[object Object],[object Object]

[object Object],[object Object],[object Object],[object Object]

[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

MTT ASSAY Absorbance of MTT Formazan, the Standard Procedure to Access Cell Activity: 0.9945 250 1.1436 300 0.8398 200 0.6240 150 0.4421 100 0.2621 50 0.0000 Blank OD at 580 nm Concentration of cell activity (µg/ml)

n Estimation of cell activity by MTT assay: 0.1790 39 0.1349 35 OD at 540 nm Percentage of activity (µg/ml)

WORK TO BE DONE ,[object Object],[object Object],[object Object],[object Object],[object Object]

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