1. Bacteriological Culture Media

2. Culture media provide optimal
conditions
for growth & multiplication of bacteria.

3. BACTERIOLOGICAL LOOP

4. INOCULATION ON CULTURE PLATES

5. According to the physical state
media may be:
Fluid Media
Solid Media
Growth is visualized as
Turbidity
Colonies
(macroscopic products of 20-30 cell divisions of
a single bacterium on a solid medium)

6. Plating-out technique
 Procedure:
Loopful of the specimen
Specimen

7. Solid media are preferable:
 Isolation in pure culture.
 Identification
(colony morphology,
effect on medium).

8. Types of culture media:
1) Simple media
2) Enriched media
3) Selective media
4) Enrichment media
5) Indicator media
6) Selective and indicator media

9. 1- SIMPLE MEDIA

10. 1- SIMPLE MEDIA
a- Nutrient broth
Meat
Extract

11. SEA WEEDS
Sea weeds

12. AGAR POWDER

13. 1- SIMPLE MEDIA
b- Nutrient agar
Nutrient Broth
+
2% agar-agar

14. 1-SIMPLE MEDIA
Staphylococcus aureus on nutrient agar (golden yellow endopigment)

15. 1- SIMPLE MEDIA
Ps. aeruginosa on nutrient agar (greenish exopigment)

16. 1-SIMPLE MEDIA
Proteus on nutrient agar (swarming growth)

17. 2-ENRICHED MEDIA
a- Blood agar ( also Indicator Medium)
Nutrient agar
+
5-10% Blood

18. 2- ENRICHED MEDIA
Beta hemolysis on blood agar

19. 2- ENRICHED MEDIA
Alpha hemolysis on blood agar

20. 2- ENRICHED MEDIA
Non hemolytic organism on blood agar

21. 2- ENRICHED MEDIA
Blood agar

22. 2- ENRICHED MEDIA
b-Chocolate agar
Heated Blood agar
Haemoglobin
Heat
Haematin

23. 3-SELECTIVE MEDIA
Lowenstein Jensen medium for M. tuberculosis
Beaten Eggs
+
Mineral salts
+
Malachite Green

24. 4- ENRICHMENT MEDIA
Selenite F Broth

25. 5- Differential (indicator) media
Triple Sugar Iron (TSI) agar
TSI agar

26. 6- SELECTIVE INDICATOR (DIFFERENTIAL) MEDIA
MacConkey’s medium
Bile Salts
+
Lactose
+
Neutral Red

27. 6- SELECTIVE INDICATOR (DIFFERENTIAL) MEDIA
MacConkey’s medium

28. Blood Culture
For isolation of bacteria from
blood samples in cases of
bacteraemia.

29. Blood culture technique
(2)
Added to
50 – 100 ml
fluid medium
(broth)
(1)
5 – 10 ml blood
(4)
Bacterial
Growth
(3)
Subculture on
Solid medium

30. Why use Large volume of broth???
1q111
1- Reduction of conc. of antibacterial
constituents in blood
2- Multiplication of bacteria
3- Neutralization of antibiotics

31. Anaerobic Cultivation
-For obligate anaerobes
Achieved by: - exclusion of oxygen
- addition of reducing substance

32. ANAEROBIC MEDIA
a- Robertson’s cooked meat medium

33. ANAEROBIC MEDIA
b- Thioglycollate Broth

34. GAS PAK SYSTEM FOR ANAEROBIC CULTIVATION

35. GAS PAK SYSTEM FOR ANAEROBIC CULTIVATION

36. MCQs

37. 1) MacConkey’s medium:
a) Is an enriched medium.
b) Is an enrichment medium.
c) Contains phenol red as a pH indicator.
d) Gives pink colonies by lactose fermenting
bacteria.
e) Gives green colonies by lactose non-fermenting
bacteria.

38. 2) Blood culture technique is used to
diagnose:
a) Sore throat
b) Conjunctivitis
c) Skin infection
d) Sinusitis
e) Bacteraemia

39. 3) All are correct about enrichment media
except:
a)They are solid media.
b) Blood agar is not considered an enrichment
medium.
c)They have selective properties.
d)They help multiplication of certain species and
suppress others.
e)They include selenite broth.

40. 4) All are correct about chocolate agar
EXCEPT:
a) It is an enrichment medium.
b) It contains heated blood.
c) It is used to grow fastidious organisms.
d) It is an enriched medium.
e) It is brown in colour.

41. 5) One of the following is used to grow strict
anaerobes:
a) Selenite broth
b) MacConkey’s medium
c) Blood agar
d) Lowenstein-Jensen medium
e) Robertson’s cooked meat medium

42. 6) When strict anaerobes are grown in the
Gaspak system:
a)They must be inoculated on cooked meat medium.
b)They can be grown on blood agar plates.
c) 50% carbon dioxide must be added.
d) The hydrogen released acts as a catalyst.
e)The palladium in the system acts as a nutrient.

43. Thank You For Your Attention