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Objective 5 and 8 (chickpea)
   Workplan for 2009-10




          Pooran Gaur
On behalf of TL-II Chickpea Team
Project locations and partners

State     Districts    NARS partners
Andhra    Kurnool &    •  Acharya NG Ranga Agricultural
Pradesh   Prakasam        University (ANGRAU), Hyderabad
                       •  AP State Seeds Development
                          Corporation (APSSDC)
                       •  National Seed Corporation (NSC)
Karnataka Gulbarga &   •  University of Agricultural Sciences
          Dharwad         (UAS), Dharwad
                       •  UAS, Raichur
                       •  Karnataka State Seeds Corporation
                          (KSSC)
Activity 5.1: Identify and enhance adoption of farmer and
market preferred chickpea cultivars in water-limited
areas
   •  FPVS have been completed and 3-4 farmer and
      market preferred varieties identified at each
      location
   •  NPT trials will be conducted on two desi (ICCV
      97105, ICCV 00108) and one kabuli (ICCV 00305)
      lines in Tanzania and on two desi (ICCV 97105,
      ICCV 00108) and two kabuli (ICCV 92318, ICCV
      00305) lines in Kenya
   •  Activities on breeder seed production will be
      carried out under Objective 8
Activity 5.2: Develop improved chickpea
germplasm with enhanced tolerance to drought,
resistance to fusarium wilt and market preferred
seed traits
   •  Over 700 desi and 400 kabuli breeding lines
      will be evaluated for phenology, grain yield,
      seed traits and resistance to fusarium wilt
      at ICRISAT, Patancheru.
   •  135 advanced breeding lines (80 desi + 55
      kabuli), ICSN-Desi and ICSN-Kabuli will be
      evaluated by NARS partners in India (3
      locations) and ESA (2 locations)
Activity 5.2

•  Evaluation of over 250 progenies derived from
   crosses between popular cultivars (ICCC 37, JG 11,
   JG 130) and drought tolerant germplasm (ICC 4958
   and ICC 8261) at ICRISAT, Patancheru.
MABC for improving root traits
                                      (from TL-I)
Crosses:          3 Cultivars x 2 Donors for root traits        Donors
BC1:                      Cultivar x F1
                                   ↓
                                BC1F1
BC 2:                      Cultivar x BC1F1
                                    ↓
                                 BC2F1
           Subjected to foreground and background selection

BC3:                     Cultivar x BC2F1                       Cultivars
                             As in BC 2
                                  ↓
                               BC3F1
        Selected heterozygous plants for QTL-linked markers
            and over 90% genome of the recurrent parent           JG 11
                                  ↓
                               BC3F2
        Selected homozygous plants for QTL-linked markers
                                  ↓
                               BC3F3
2009/10                  Seed multiplication                  Chefe       KAK 2
                                  ↓
2010/11          Multilocation evaluation BC3F4 lines
Marker-assisted recurrent selection (MARS)
              using good-by-good crosses for improving
                yield under moisture stress conditions
                              (from TL-I)
     Reselection generation after generation, with interbreeding of
     selects (selected using QTL-associated molecular markers) to
     provide opportunity for genetic recombination and cumulate
     favorable alleles

                 2 crosses                         1 cross
2009/10          Genotyping of F3 completed;      Genotyping of F3 plants
                 F4 grown for seed multiplication

2010/11          Multilocation evaluation        F4 (Seed multiplication)
                 of F5 progenies and
                 QTL analysis

                 Selection and inter-crossing     Multilocation evaluation
                 of F3s                           of F5 progenies and
                                                  QTL analysis
• 
Activity 5.2

Enhance resistance to pod borer by combining
different mechanisms of resistance from the cultivated
and the wild species
  •  Promising F6 progenies from C. arietinum (ICC
     506) x C. reticulatum (IG 72933) will be further
     evaluated for resistance to pod borer.
  •  Seven C. arietinum (JG 130, JAKI 9218, JG 11, KAK
     2, JGK 1, ICC 17109, ICC 506) x C. reticulatum (IG
     72933) crosses will be advanced to F3.
Activity 5.3: Enhance capacity of NARS in chickpea
improvement research and development and provide
training to farmers in improved chickpea production
technology
 Development of infrastructure facilities
 DZARC-        •  Upgrading irrigation facilities
 Ethiopia
 LZARDI-       •  Fencing of experimental area
 Tanzania      •  Establishing of fusarium wilt sick plot
               •  Renovation of greenhouse
 KARI-Kenya    •  Establishing of fusarium wilt sick plot
Activity 5.3

Training of farmers and extension personnel
 •    A calendar of training programs developed for each
      location
 •    About 100 extension personnel will be provided
      training on improved technologies for chickpea
      production in India and ESA
 •    Over 3000 farmers in India and 1000 farmers in ESA
      will be provided training on chickpea crop and seed
      production technologies, and seed processing,
      storage and marketing.
Degree students
Country    Student          Degree Research area
                            program
Ethiopia   Tadesse Sefera   M Sc    Molecular characterization
           Gela                     of chickpea cultivars
Kenya      Peter Kaloki     M Sc    Heat tolerance in chickpea
India      Tosh Garg        PhD     Molecular mapping of
                                    fusarium wilt, ascochyta
                                    blight, and botrytis grey
                                    mould resistance in
                                    chickpea
Objective 8
Developing sustainable seed production and
  delivery system for reaching the poor in
    drought-prone areas of SSA and SA

            Workplan for India
Improving the availability of quality seed and
       linking formal and informal seed sectors

•  About 150 t breeder seed and 600 t other classes of
   seed (foundation, certified and TL) will be produced
   by project partners
•  About 400 farmers will be trained as seed growers
   and each farmer will be provided 15 to 25 kg quality
   seed to promote informal seed system.
•  About 20 farmers from selected villages in Andhra
   Pradesh will be linked to APSSDC or NSC for
   production of Foundation/Certified seed.
•  Two Seed Producing Farmers’ Groups will be
   established in Karnataka and linked to KSSC and
   NSP of UAS-Raichur.
Enhancing local capacity to produce,
              deliver, store and market seed


•  Training of farmers, NGOs and extension officials in
   seed production, processing, storage and marketing
•  Training of local seed traders in proper seed
   handling and storage
•  Publication of chickpea seed production manual in
   English and local languages (Telugu and Kannada)
Enhancing local-level awareness of
          improved varieties (demand creation)

•    TV talks
•    Radio talks
•    News and popular articles in local newspapers
•    Field days
•    Farmer Fairs
•    Distribution of seed samples
Workplan
for
ESA

Project partners in ESA


Country    Partners
Ethiopia   •  Debre Zeit (DZARC)
           •  Holetta (HARC)
           •  Kulumsa (KARC)
           •  Debre Birhan
              (ARARI)
Tanzania   LZARDI, Ukiriguru
Activity 8.2.1: Improving the availability of initial
Foundation seed by NARS and other public sector
as well as private sector partners

Ethiopia: 12 t of foundation seed of Habru,
            Arerti and Ejere

Tanzania: 3 t of foundation seed of ICCV 00305,
     ICCV 00108, ICCV 97105 and ICCV 92318
Activity 8.2.2: Designing, testing and implementing seed
production programs, tailored to varying client needs
   •  Continue links with NGOs and CBO partners
      involved in seed production and distribution to
      smallholder farmers in Tanzania (2 NGOs) and
      Ethiopia(3 NGOs)
   •  Farmer cooperative in Ethiopia will be used for
      seed production and distribution
   •  Pro-poor seed delivery systems such as seed
      revolving scheme, community seed banks, farmer
      field schools will be continued
Activity 8.2.3: Designing, testing and implementing
seed diffusion, marketing, and institutional
arrangements to enhance seed delivery tailored to
varying client needs
 •  Farmer marketing groups established in Tanzania
    will be continued
 •  In Ethiopia, linkages with Agricultural
    Commodities and Supplies for seed multiplication
    and marketing
Activity 8.2.4: Enhancing local level capacity to produce,
deliver, store and market seed
   •  Training to farmers (100) and extension staff (15)
      on seed production, processing, proper storage
      and market channels for seed both in Ethiopia and
      Tanzania
   •  Complete printing of seed production manuals in
      Amharic and Swahili
Activity 8.2.5: Enhancing local level awareness of
released varieties
 •  Organizing field days at seed production and
    scaling up sites, seed fairs, demonstrations to
    create awareness about new varieties
 •  Flyers describing new varieties in local language
    and English will be printed and distributed
 •  Mass communication through Radio, TV, news
    papers will be continued
Proposed plan for phase II

Target countries: Same as in Phase I
                  (ESA: Ethiopia, Tanzania and Kenya
                  SA: India)
Target regions:     Expansion of activities to new districts
                    (1 to 2 districts in each country)

Activity 1:   Identification of farmer and market
                            preferred cultivars

Emphasis in FPVS will be in the new target areas
Activity 2: Targeted breeding

•  Use newly identified sources of resistance/tolerance
   for developing improved breeding lines with
   enhanced drought tolerance, resistance to fusarium
   wilt, and market-preferred seed traits.

•  Develop ascochyta blight resistant breeding lines for
   Ethiopia and Kenya – new activity.

•  Use marker-assisted breeding (MABC & MARS) for
   improving yield under moisture stress conditions in
   target environments (linked to TL-I).
Activity 3: Capacity building
•  Capacity building of researchers and technicians in
   using genomic tools in chickpea improvement (in
   partnership with TL-I).
•  Knowledge empowerment of extensional personnel
   and farmers in chickpea crop and seed production
   technologies, seed processing, storage and
   marketing
•  Degree students (MSc/PhD)
Chickpea seed system
Activity: Enhancing access of farmers to quality seed
•  Validate and promote promising seed system
   models identified in phase I
33 Pooran Gaur Objective5 Work Plan

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33 Pooran Gaur Objective5 Work Plan

  • 1. Objective 5 and 8 (chickpea) Workplan for 2009-10 Pooran Gaur On behalf of TL-II Chickpea Team
  • 2. Project locations and partners State Districts NARS partners Andhra Kurnool & •  Acharya NG Ranga Agricultural Pradesh Prakasam University (ANGRAU), Hyderabad •  AP State Seeds Development Corporation (APSSDC) •  National Seed Corporation (NSC) Karnataka Gulbarga & •  University of Agricultural Sciences Dharwad (UAS), Dharwad •  UAS, Raichur •  Karnataka State Seeds Corporation (KSSC)
  • 3. Activity 5.1: Identify and enhance adoption of farmer and market preferred chickpea cultivars in water-limited areas •  FPVS have been completed and 3-4 farmer and market preferred varieties identified at each location •  NPT trials will be conducted on two desi (ICCV 97105, ICCV 00108) and one kabuli (ICCV 00305) lines in Tanzania and on two desi (ICCV 97105, ICCV 00108) and two kabuli (ICCV 92318, ICCV 00305) lines in Kenya •  Activities on breeder seed production will be carried out under Objective 8
  • 4. Activity 5.2: Develop improved chickpea germplasm with enhanced tolerance to drought, resistance to fusarium wilt and market preferred seed traits •  Over 700 desi and 400 kabuli breeding lines will be evaluated for phenology, grain yield, seed traits and resistance to fusarium wilt at ICRISAT, Patancheru. •  135 advanced breeding lines (80 desi + 55 kabuli), ICSN-Desi and ICSN-Kabuli will be evaluated by NARS partners in India (3 locations) and ESA (2 locations)
  • 5. Activity 5.2 •  Evaluation of over 250 progenies derived from crosses between popular cultivars (ICCC 37, JG 11, JG 130) and drought tolerant germplasm (ICC 4958 and ICC 8261) at ICRISAT, Patancheru.
  • 6. MABC for improving root traits (from TL-I) Crosses: 3 Cultivars x 2 Donors for root traits Donors BC1: Cultivar x F1 ↓ BC1F1 BC 2: Cultivar x BC1F1 ↓ BC2F1 Subjected to foreground and background selection BC3: Cultivar x BC2F1 Cultivars As in BC 2 ↓ BC3F1 Selected heterozygous plants for QTL-linked markers and over 90% genome of the recurrent parent JG 11 ↓ BC3F2 Selected homozygous plants for QTL-linked markers ↓ BC3F3 2009/10 Seed multiplication Chefe KAK 2 ↓ 2010/11 Multilocation evaluation BC3F4 lines
  • 7. Marker-assisted recurrent selection (MARS) using good-by-good crosses for improving yield under moisture stress conditions (from TL-I) Reselection generation after generation, with interbreeding of selects (selected using QTL-associated molecular markers) to provide opportunity for genetic recombination and cumulate favorable alleles 2 crosses 1 cross 2009/10 Genotyping of F3 completed; Genotyping of F3 plants F4 grown for seed multiplication 2010/11 Multilocation evaluation F4 (Seed multiplication) of F5 progenies and QTL analysis Selection and inter-crossing Multilocation evaluation of F3s of F5 progenies and QTL analysis • 
  • 8. Activity 5.2 Enhance resistance to pod borer by combining different mechanisms of resistance from the cultivated and the wild species •  Promising F6 progenies from C. arietinum (ICC 506) x C. reticulatum (IG 72933) will be further evaluated for resistance to pod borer. •  Seven C. arietinum (JG 130, JAKI 9218, JG 11, KAK 2, JGK 1, ICC 17109, ICC 506) x C. reticulatum (IG 72933) crosses will be advanced to F3.
  • 9. Activity 5.3: Enhance capacity of NARS in chickpea improvement research and development and provide training to farmers in improved chickpea production technology Development of infrastructure facilities DZARC- •  Upgrading irrigation facilities Ethiopia LZARDI- •  Fencing of experimental area Tanzania •  Establishing of fusarium wilt sick plot •  Renovation of greenhouse KARI-Kenya •  Establishing of fusarium wilt sick plot
  • 10. Activity 5.3 Training of farmers and extension personnel •  A calendar of training programs developed for each location •  About 100 extension personnel will be provided training on improved technologies for chickpea production in India and ESA •  Over 3000 farmers in India and 1000 farmers in ESA will be provided training on chickpea crop and seed production technologies, and seed processing, storage and marketing.
  • 11. Degree students Country Student Degree Research area program Ethiopia Tadesse Sefera M Sc Molecular characterization Gela of chickpea cultivars Kenya Peter Kaloki M Sc Heat tolerance in chickpea India Tosh Garg PhD Molecular mapping of fusarium wilt, ascochyta blight, and botrytis grey mould resistance in chickpea
  • 12. Objective 8 Developing sustainable seed production and delivery system for reaching the poor in drought-prone areas of SSA and SA Workplan for India
  • 13. Improving the availability of quality seed and linking formal and informal seed sectors •  About 150 t breeder seed and 600 t other classes of seed (foundation, certified and TL) will be produced by project partners •  About 400 farmers will be trained as seed growers and each farmer will be provided 15 to 25 kg quality seed to promote informal seed system. •  About 20 farmers from selected villages in Andhra Pradesh will be linked to APSSDC or NSC for production of Foundation/Certified seed. •  Two Seed Producing Farmers’ Groups will be established in Karnataka and linked to KSSC and NSP of UAS-Raichur.
  • 14. Enhancing local capacity to produce, deliver, store and market seed •  Training of farmers, NGOs and extension officials in seed production, processing, storage and marketing •  Training of local seed traders in proper seed handling and storage •  Publication of chickpea seed production manual in English and local languages (Telugu and Kannada)
  • 15. Enhancing local-level awareness of improved varieties (demand creation) •  TV talks •  Radio talks •  News and popular articles in local newspapers •  Field days •  Farmer Fairs •  Distribution of seed samples
  • 17. Project partners in ESA Country Partners Ethiopia •  Debre Zeit (DZARC) •  Holetta (HARC) •  Kulumsa (KARC) •  Debre Birhan (ARARI) Tanzania LZARDI, Ukiriguru
  • 18. Activity 8.2.1: Improving the availability of initial Foundation seed by NARS and other public sector as well as private sector partners Ethiopia: 12 t of foundation seed of Habru, Arerti and Ejere Tanzania: 3 t of foundation seed of ICCV 00305, ICCV 00108, ICCV 97105 and ICCV 92318
  • 19. Activity 8.2.2: Designing, testing and implementing seed production programs, tailored to varying client needs •  Continue links with NGOs and CBO partners involved in seed production and distribution to smallholder farmers in Tanzania (2 NGOs) and Ethiopia(3 NGOs) •  Farmer cooperative in Ethiopia will be used for seed production and distribution •  Pro-poor seed delivery systems such as seed revolving scheme, community seed banks, farmer field schools will be continued
  • 20. Activity 8.2.3: Designing, testing and implementing seed diffusion, marketing, and institutional arrangements to enhance seed delivery tailored to varying client needs •  Farmer marketing groups established in Tanzania will be continued •  In Ethiopia, linkages with Agricultural Commodities and Supplies for seed multiplication and marketing
  • 21. Activity 8.2.4: Enhancing local level capacity to produce, deliver, store and market seed •  Training to farmers (100) and extension staff (15) on seed production, processing, proper storage and market channels for seed both in Ethiopia and Tanzania •  Complete printing of seed production manuals in Amharic and Swahili
  • 22. Activity 8.2.5: Enhancing local level awareness of released varieties •  Organizing field days at seed production and scaling up sites, seed fairs, demonstrations to create awareness about new varieties •  Flyers describing new varieties in local language and English will be printed and distributed •  Mass communication through Radio, TV, news papers will be continued
  • 23. Proposed plan for phase II Target countries: Same as in Phase I (ESA: Ethiopia, Tanzania and Kenya SA: India) Target regions: Expansion of activities to new districts (1 to 2 districts in each country) Activity 1: Identification of farmer and market preferred cultivars Emphasis in FPVS will be in the new target areas
  • 24. Activity 2: Targeted breeding •  Use newly identified sources of resistance/tolerance for developing improved breeding lines with enhanced drought tolerance, resistance to fusarium wilt, and market-preferred seed traits. •  Develop ascochyta blight resistant breeding lines for Ethiopia and Kenya – new activity. •  Use marker-assisted breeding (MABC & MARS) for improving yield under moisture stress conditions in target environments (linked to TL-I).
  • 25. Activity 3: Capacity building •  Capacity building of researchers and technicians in using genomic tools in chickpea improvement (in partnership with TL-I). •  Knowledge empowerment of extensional personnel and farmers in chickpea crop and seed production technologies, seed processing, storage and marketing •  Degree students (MSc/PhD)
  • 26. Chickpea seed system Activity: Enhancing access of farmers to quality seed •  Validate and promote promising seed system models identified in phase I