AB SCIEX 2014: A Year in Review, A Year to Remember!

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Description month by month of the exciting year of 2014 for AB SCIEX, a year full of innovations, new products and discoveries, events, and much more!

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AB SCIEX 2014
A Year in Review and a Year to Remember!

2 © 2014 AB SCIEX
January
Halal Meat Authenticity Testing Reassures Consumers of No
Pig or Horse Contamination.

3 © 2014 AB SCIEX
February
A Glass of Wine Could Trigger
Allergies, Increasing the Need
for ‘Wine-Testing’ Before ‘Wine-
Tasting’.
Innovation delivered at
PITTCON 2014: Rainer
Blair, President of AB
SCIEX announced
availability of
breakthrough technology
CESI-MS.

4 © 2014 AB SCIEX
April
AB SCIEX Announces
Immunosuppressants Kit for
Use by Doctors in Europe to
Help Care for Organ Transplant
Patients.
Analytica 2014 in Munich

5 © 2014 AB SCIEX
May
AB SCIEX establishes a partnership with The Center for
Neuroscience and Cell Biology of University of Coimbra, in
Portugal.

6 © 2014 AB SCIEX
June
ASMS 2014 in Baltimore: next-generation Proteomics platform
revealed, with TripleTOF® 6600 System and SWATH™
Acquisition 2.0. And Triple Quad™ 3500 System, for food and
environmental testing laboratories.

7 © 2014 AB SCIEX
July
Full House for the Ruedi Aebersold talk on SWATH™ at the
Fudan University! and first China release of the new TripleTOF®
6600.

8 © 2014 AB SCIEX
August
Sciex Biopharma Solutions
Mobile Lab Tour
IMSC in Geneva

9 © 2014 AB SCIEX
September
AB SCIEX scientists take the « Dry » Ice Bucket Challenge!

10 © 2014 AB SCIEX
October
HUPO 2014 in Madrid
AB SCIEX and Illumina
Collaborate to Create World’s
First Multi-omics Cloud-
Computing Environment.

11 © 2014 AB SCIEX
Awards
Dr. Subhasish “Babu”
Purkayastha wins HUPO 2014
Science and Technology
Award and becomes second
AB SCIEX team member to be
recognized for the
commercialization of
transformative chemistries
such as iTRAQ® reagents.
AB SCIEX Wins New Product
Innovation Award from Frost &
Sullivan in Asia Pacific.

12 © 2014 AB SCIEX
November
AB SCIEX expands software
capability to include
DiscoveryQuant™ 3.0 for
the High Throughput
Pharmaceutical Laboratory.
Germany’s Swimming Professor
chooses AB SCIEX QTRAP®
technology for ground-breaking
Rhine river water
decontamination.

13 © 2014 AB SCIEX
December
More than 50 « How-To »
videos available on Youtube,
and free eLearning center on
the website.
AB SCIEX receives « pastry
art » from customer in Vienna!

14 © 2014 AB SCIEX
See you in 2015, with more Discoveries, more
Challenges, more Content, and more Fun!

15 © 2014 AB SCIEX
Trademarks/Licensing
For Research Use Only. Not for use in diagnostic procedures.
© 2014 AB SCIEX. The trademarks mentioned herein are
the property of AB Sciex Pte. Ltd. or their respective owners.
AB SCIEX™ is being used under license. All rights reserved.
Information subject to change without notice.

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La spectrométrie de masse en tandem est devenue un outil essentiel pour les applications cliniques et la recherche biomédicale impliquant l’analyse de biomarqueurs. Contrairement aux méthodes classiques de dosage immunologique, l’analyse par chromatographie liquide couplée à la spectrométrie de masse (LC-MS/MS) permet une analyse très sélective et spécifique de composés multiples en un seul passage, conduisant à une plus grande confiance dans les résultats et permettant de nouvelles découvertes. La LC-MS/MS est idéalement adaptée pour identifier les hormones stéroïdes, la vitamine D et ses métabolites, des peptides ou des protéines et d'autres composés dans des matrices complexes tels que le sang, l'urine, la salive et les lysats céllulaires. Aujourd'hui, la LC-MS/MS est également la méthode préférée de l'analyse médico-légale surpassant les techniques d'analyse traditionnelles - à la fois à des fins de dépistage et de confirmation. Cette présentation vous présentera comment SCIEX pourrait contribuer à améliorer le monde dans lequel nous vivons en permettant aux scientifiques et aux analystes de laboratoire de trouver des réponses aux défis analytiques complexes auxquels ils sont confrontés.

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Recently (November 2014), threats in the form of letters were sent to farming and dairy industry leaders in New Zealand. The letters were accompanied by small packages of milk powder that were shown to contain a concentrated form of the pesticide 1080 (sodium fluoroacetate). The sender demanded that the New Zealand government stop using 1080 for pest control. Sodium fluoroacetate is used to protect New Zealand’s native flora and fauna against introduced pests like possums and ferrets. Opponents, however, argue that it also kills native animals and contaminates the environment.1-2 Such criminal threats are a potential danger and weaken consumers’ trust in the food supply chain. Accurate and reliable analytical methods are needed to monitor food ingredients and final products to ensure food safety in light of this threat. Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) is an ideal analytical technique to detect polar analytes in complex food samples. Here we present first results of method development to detect sodium fluoroacetate in milk and infant formula. The sample preparation protocol consists of a simple acetonitrile extraction and defatting using hexane. LC separation was achieved using a HILIC column in normal phase mode. The mass spectrometer was operated in Multiple Reaction Monitoring (MRM) mode. In MRM mode the transition of a molecular ion into a characteristic fragment ion is monitored. The monitoring of more than a single fragment ion allows not only quantitation but also highly confident identification based on the ratio between quantifier and qualifier transitions. Initial studies show that sodium fluoroacetate can be detected at concentrations below 1 ng/mL (below 10 ng/mL in matrix) using the SCIEX QTRAP® 4500 system, with good accuracy and repeatability. Linearity for quantitation was achieved over 3 orders of magnitude (0.1 to 100 ng/mL). Future experiments are planned to further increase sensitivity, simplify sample preparation and to include an internal standard to correct low recoveries and matrix effects.

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Transport plays an important role in the absorption, distribution, and elimination of a variety of drugs. In recent years, a large number of transporters, both efflux (ATP-binding cassette (ABC) family) and influx (solute carrier (SLC) family members) have been identified and well characterized in vitro. However, the abundance of these transporters in the hepatocyte and cell lines as well as in the tissues such as intestine, liver, and kidney has not been accurately quantitated due to technical challenges. This work aims to build a robust liquid chromatography-mass spectrometry (LC-MS) workflow on the SCIEX TripleTOF® 6600 platform to enable the quantitation of a variety of SLC and ABC drug transporters expressed in the hepatocyte and cell line plasma membranes.

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Heterogeneity and high molecular weight species pose challenges for analytical scientists and both of these problems converge with Antibody Drug Conjugates (ADCs). ADCs are one of the fastest growing segments of the biotherapeutic pipeline, with hundreds of therapeutics in development. Early in development, analysts are tasked with providing rapid feedback to their synthetic chemists on how well a conjugation strategy may have worked. Later in development as process development accelerates, analysts need means to rapidly confirm that the product has maintained its integrity, for example in formulation. Therefore there is always pressure to respond rapidly to the demands of multiple departments. In this Technical Brief we illustrate how TripleTOF® technology coupled with SelexION™, Eksigent ekspert™ MicroLC, and BioPharmaView™ software or PeakView® software can provide answers where they are needed, fast. Assays that may have needed days to complete are now ready to report within less than an hour.

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Following the Food Standards Agency’s (FSA) announcement in January that horse and pig DNA had been identified in beef products sold by several supermarket chains, further testing across Europe and beyond has revealed widespread incidences of such contamination.1 However, most testing methods are based on detection of species-specific DNA in meat, using the polymerase chain reaction (PCR) – which does not detect or identify proteins. This is a concern because DNA can be easily disrupted or removed during standard meat processing and food manufacturing. As a result, horse tissue or other contaminants remain undetected in food samples, despite strong presence of the contaminating proteins. An alternative protein-based method, ELISA (enzyme-linked immunosorbent assay), can be used to complement DNA testing, but this method has limitations, including that it detects only one part of the protein and not multiple protein markers. The LC-MS/MS-based method presented offers a more accurate and reliable approach to meat speciation than PCR or ELISA-based techniques or other indirect methods, and also allows for the detection of veterinary drug residues in the same analysis, which is not possible by ELISA or PCR. The method was developed using an Eksigent ekspert™ microLC 200 UHPLC system coupled with a SCIEX QTRAP® 5500 LC/MS/MS system. The method uses multiple reaction monitoring (MRM) to detect peptide markers for horse and is capable of providing sequence information by acquiring an enhanced product ion (EPI) scan for each triggering MRM which can be used to further confirm the peptide’s / proteins and therefore the species identity. This gives greater confidence for food testing when distinguishing between species; for example horse and beef proteins may differ by as little as one or two amino acids. At the same time it is also possible to detect and quantify veterinary drug residues using the same extraction method and LC conditions by simply adding additional MRM transitions to the method. Here the nonsteroidal anti-inflammatory drug (NSAID) BUTE was detected in meat samples.

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Cotton crops are vulnerable to a variety of sucking pests, which can severely impact plant health, yield, and fiber quality. These pests primarily feed on plant sap, extracting nutrients directly from the plant's vascular system. Here's a breakdown of some of the most significant sucking pests in cotton cultivation: Aphids: Cotton aphids or melon aphids can cause direct damage by sucking sap and indirect damage by secreting honeydew, which encourages sooty mold growth. This can interfere with photosynthesis and weaken the plant. Aphids can also transmit viral diseases. Whiteflies: Two species, the silverleaf whitefly and the bandedwinged whitefly, are particularly troublesome. They not only suck sap from the underside of leaves, causing yellowing and leaf drop, but their honeydew excretion promotes sooty mold and they can transmit several plant viruses. Thrips: While thrips can chew on plants, their primary damage to cotton is through sucking. They attack the cotton plant during its seedling stage, which can stunt growth and reduce vigor. Thrips are also capable of transmitting the Cotton Bud disease. Spider Mites: These are not insects but arachnids. Spider mites, such as the two-spotted spider mite, suck cell contents from the leaves, leading to speckled discoloration and potentially significant leaf loss if infestations are severe. Leafhoppers: Including various species, leafhoppers can cause direct damage through feeding, which results in leaf curling and stunted growth. They can also be vectors for plant diseases. Mealybugs: These pests are less common but can be problematic, especially in clustered planting conditions. They suck sap and secrete honeydew, which leads to sooty mold. Mealybugs can also spread viruses. Stink Bugs: Although primarily known for their chewing mouthparts, certain stink bugs can cause damage similar to sucking pests by injecting saliva into the plant and sucking out nutrients, leading to boll damage and stained lint. Management Strategies: Cultural Controls: This includes practices such as crop rotation, using resistant varieties, and managing planting and harvesting times to avoid peak pest populations. Biological Controls: Beneficial insects like lady beetles, lacewings, and predatory mites can naturally control sucking pest populations. Parasitic wasps also play a role in controlling aphid and whitefly populations. Chemical Controls: Insecticides may be used but should be chosen carefully to minimize resistance development and preserve beneficial insects. Systemic insecticides can be particularly effective against sucking pests. Integrated Pest Management (IPM): Combining multiple control strategies based on monitoring and thresholds to apply the most effective and environmentally sensitive approach. Effective management of sucking pests in cotton requires a thorough understanding of the pest species present, their life cycles, and the ecological balance of the field environment.

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Molecular markers are identifiable DNA sequences used to locate genes associated with specific traits or genetic conditions. A molecular marker is a specific gene fragment present at a specific position called ‘locus’ (pleural loci) in the genome of a cell. In the pool of unknown DNA or in a whole chromosome, these molecular markers help in identification of particular sequence of DNA at particular location.

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AB SCIEX 2014: A Year in Review, A Year to Remember!

1. AB SCIEX 2014 A Year in Review and a Year to Remember!

3. 3 © 2014 AB SCIEX February A Glass of Wine Could Trigger Allergies, Increasing the Need for ‘Wine-Testing’ Before ‘Wine- Tasting’. Innovation delivered at PITTCON 2014: Rainer Blair, President of AB SCIEX announced availability of breakthrough technology CESI-MS.

6. 6 © 2014 AB SCIEX June ASMS 2014 in Baltimore: next-generation Proteomics platform revealed, with TripleTOF® 6600 System and SWATH™ Acquisition 2.0. And Triple Quad™ 3500 System, for food and environmental testing laboratories.

11. 11 © 2014 AB SCIEX Awards Dr. Subhasish “Babu” Purkayastha wins HUPO 2014 Science and Technology Award and becomes second AB SCIEX team member to be recognized for the commercialization of transformative chemistries such as iTRAQ® reagents. AB SCIEX Wins New Product Innovation Award from Frost & Sullivan in Asia Pacific.

12. 12 © 2014 AB SCIEX November AB SCIEX expands software capability to include DiscoveryQuant™ 3.0 for the High Throughput Pharmaceutical Laboratory. Germany’s Swimming Professor chooses AB SCIEX QTRAP® technology for ground-breaking Rhine river water decontamination.

15. 15 © 2014 AB SCIEX Trademarks/Licensing For Research Use Only. Not for use in diagnostic procedures. © 2014 AB SCIEX. The trademarks mentioned herein are the property of AB Sciex Pte. Ltd. or their respective owners. AB SCIEX™ is being used under license. All rights reserved. Information subject to change without notice.

AB SCIEX 2014: A Year in Review, A Year to Remember!

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