Customized Antibody development service for protein, peptides and small molecules. www.kmshc.com/pdf/Specialist_Biosimilar_Service.pdf‎

1. 1. Polyclonal Antibody Development Service
MTR offering a variety of Polyclonal Antibody Services to fit your research and production
needs. we develop polyclonal antibodies to targets including parasites, cancer-specific
surface antigens, hormones, growth factors and their receptors, glycoproteins, peptides,
proteins and their modification sites (mutation site-specific, phosphorylation site-specific,
cleavage site-specific, etc), and other antigens such as small-molecule compounds and DNA.
We have created antibodies for various applications, such as functional assays, antibodies for
immunoprecipitation, IHC, IF, flow cytometry, western blot, and EIA.
Project Timeline:
4-6 weeks
Deliverables:
We will deliver approximately 1 ml of mice serum or 5 ml of Rabbit serum with guaranteed
titer at least 1:10,000 (Range 1:10,000 to 1:100,000).
2. Monoclonal Antibody Development Services
MTR offer mouse hybridoma development technology for producing custom monoclonal
antibodies from synthesized peptide or recombinant protein antigens. Novel immunization
strategies, antigen design, and screening technologies are essential for the successful
generation of monoclonal hybridoma cells lines. Our immunization protocols take advantage
of using altered immunization routes, combinations of ligand carriers and dosing strategies to
produce the most diverse monoclonal antibody population possible. Antigens must be
carefully designed, especially when dealing with peptides, to generate a robust reagent that is
able to recognize conformational changes of the native protein in assay conditions.
Highlights:
Monoclonal antibodies - Robust protocol options for immunizing mice, ELISA-titer test
bleeds, creating fusions, screening hybridomas, and delivering cell lines
Customizable and adaptable - each protocol can be expanded or modified in a variety of
ways to service specific needs for final scale, specificity or assay validation
Project Timeline:
3-4 months

2. Deliverables:
We will deliver two positive Hynridoma clone and 5ml of culture supernatant.
3. Antibody and Protein Purification Services
Services and Offerings
MTR provide purification service antibodies and protein utilizing a wide array of protein
purification methods; from research (mg) to scale-up (grams) quantities. We also offer low
endotoxin production for in-vivo use. Our goal is to find or develop the antibody purification
procedure which provides the best balance between yield, purity, and cost. Available
Antibody Purification services and methods include:
Ammonium Sulphate Precipitation
This method is useful for concentration and partial purification of antibodies from most
sources and all species. Although, on its own the yields of antibody are impure, it is the
method of choice when combined with ion exchange for large volumes of anti-sera. It is not
recommended for purification of tissue culture supernatant.
Protein A
This process binds the Fc region of major sub-classes of mouse and rat IgG to varying
degrees. The results with protein A are usually high purity and high yields. It is useful for
large-scale purifications of tissue culture supernatants.
Protein G
Protein G can be used for purifying antibody from most sources. Like protein A, the results
are usually of high purity and high yield. It is applicable to a wider range of
immunoglobulins than protein A, but elution procedures can be much harsher.

3. Ion Exchange Chromatography
This method is used for all sources of antibodies. It is applicable to all lgG isotypes. It is
usually the method of choice when purifying antibodies from ascites, as the monoclonal can
be resolved from the host immunoglobulins.
Antigen Affinity
This is the method of choice when specific antibody is required from polyclonal anti-sera. It
requires pure antigen for the best results and can sometimes result in inactive antibody.
Size Exclusion
This method is appropriate for IgM antibodies from all sources. It is used when IgM
Antibodies need to be separated from polyclonal anti-sera. It is not recommended as a single
stage.
Project Timeline:
2-3 weeks
Deliverables:
Purified antibody
Project report
4. Immunoassay Development Services
MTR immunoassay development services are project-based and designed for diagnostics and
drug discovery applications. Our scientists and project managers will discuss with each client
the project design, cost estimates, and delivery specifications.
We offer three basic categories of services
 Immunoassay development service for diagnostic Kits.
 Development and Validation of ELISA of ELISA for pharmacokinetic assay of Bio
similar protein, Antibody and peptide.

4.  Development and Validation of ELISA for Immunogenicity assay for Bio similar protein,
antibody and peptide
Project Timeline:
3-4 months
Deliverables:
 Deliverables vary depending on the types of services requested and the requirements of
the assays.
 In general, we deliver cell lines, antibodies and conjugates, assay protocols, assay
reagents, assay kits, experiment records, and specific data derived from a given project.
5. Labeling of Antibody and Protein
MTR offer conjugation service for antibody, protein and peptide with following conjugate
 Traditional fluorophores such as FITC and Texas Red® dye
 Biotin
 HRP
 Alkaline phosphatase, and other enzymes
 KLH and BSA for peptide
If you don’t see the label you are interested in, just ask! In most cases we can accommodate
your request.
Sample requirements:
Synthetic peptide or amino acid sequence of the peptide
Project Timeline:
2-3 weeks
Deliverables:
Peptide carrier conjugate
Project report
6. Molecular and cell Biology Services
MTR offer reliable and economical molecular biology outsourcing, including de novo gene
synthesis, gene cloning, DNA construct, plasmid preparation, and site directed mutagenesis.

5. The services cover the entire range of gene analysis from identification and cloning to
functional studies.
Deliverables:
Cloned gene in desired vector.
7. Cell-based Bioassay Services
MTR offer service for developing the cell based assays for the determination of specific
activity and biological characterization. We have expertise and technology for the following
assay
 Cell Proliferation Assays
 Cell Based cAMP Release Assay
 Competitive Receptor Binding (Flow Cytometry)
 Reporter Gene Assay
 CDC Assay
 ADCC Assay
 Antiproliferation Assay
 Immunogenecity Assays
 Neutralizing Antibody Assays
Deliverables:
Project report