Bls 206 lecture 3

Isolation Technique ,[object Object],[object Object],[object Object],[object Object]

Mixed Culture from Raw Poultry

Streak Plate Isolation Principle ,[object Object],[object Object],[object Object],[object Object]

The Goal -Isolated Colonies to Start Pure Cultures

Can an isolated colony be considered pure? ,[object Object],[object Object],[object Object],[object Object],[object Object]

Any special considerations? ,[object Object],[object Object],[object Object],[object Object],[object Object]

Microbes will surprise you each chance they get !

Isolation Requires Aseptic Technique

Isolation Requires Aseptic Technique ,[object Object],[object Object],[object Object],[object Object],[object Object]

Quadrant 1- Streak with broad narrow strokes in the upper half of the first quarter of the plate.

Incinerate and cool the loop between the quadrants

Quadrant 2 – Rotate the plate, enter the previous streak mark one or two times and then streak the upper portion of the second quarter of the plate with broad strokes.

Quadrant 3 – Rotate the plate, enter quadrant 2 one or two times and then streak with shorter more separated strokes from the top of the quadrant to the center.

Quadrant 4 – Enter quadrant 3 and then streak with broad S-shaped motions through the center of the plate.

Culturing and Isolation Techniques ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

[object Object],[object Object],[object Object],[object Object],[object Object]

[object Object],[object Object],[object Object],[object Object]

Inoculation of a Broth Culture ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Compare your inoculated broth tube to the un-inoculated control tube to determine the amount of turbidity. The more turbid the broth The higher the bacterial count per mL of broth.

Inoculating an Agar Slant ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Streak Plates ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Pour Plating for Colony Counts ,[object Object],[object Object],[object Object],[object Object]

Calculating CFU from dilution plating results ,[object Object],[object Object],[object Object],The initial dilution is calculated As follows: So the initial dilution is 1/10 or 0.1 or 10 -1 .

[object Object],[object Object],[object Object]

[object Object],Therefore, the “Dilution factor for our example is: 10 6 and the Total Colony Forming Units (CFUs) for this sample is:

Bls 206 lecture 3

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Bls 206 lecture 3