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Biotechnology The New Frontier
Recombinant DNA ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
The Insulin Example ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Transferring Genes ,[object Object],[object Object],[object Object],[object Object]
Restriction Enzymes ,[object Object],[object Object],[object Object],[object Object]
 
Production of Recombinant DNA ,[object Object],[object Object]
 
 
Cloning a Human Gene ,[object Object],[object Object]
Forming a Recombinant Plasmid ,[object Object]
Gene Cloning ,[object Object],[object Object]
Molecular “Searching” Techniques ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Techniques ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Complimentarity and Hybridization ,[object Object],[object Object],[object Object],[object Object]
The Probe-Target Complex ,[object Object],[object Object],[object Object],[object Object]
Hybrid Types ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Features of Hybridization ,[object Object],[object Object],[object Object],[object Object],[object Object]
The Importance of Hybridization ,[object Object],[object Object],[object Object]
Locating the Target ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Steps in the Process ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Separation by Molecular Weight ,[object Object],[object Object],[object Object],[object Object]
Gel Electrophoresis ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Gel Electrophoresis
Separation of Molecules on a Gel ,[object Object],[object Object],[object Object]
Size vs. Molecular Weight ,[object Object],[object Object],[object Object],[object Object],[object Object]
Controlling for Shape ,[object Object],[object Object]
Preparation Techniques ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Determining Molecular Weight ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Use of Standards ,[object Object],[object Object],[object Object]
Staining ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Transfer to Solid Support ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Transfer via Electrophoresis Electrophoresis takes advantage of the molecules'  negative charge .
Capillary Blotting The molecules are transferred in a flow of buffer  from wet filter paper to dry filter paper.
Blocking ,[object Object],[object Object],[object Object],[object Object],[object Object]
Preparing the Probe ,[object Object],[object Object],[object Object],[object Object],[object Object]
Probes for Westerns ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Hybridization & Washing ,[object Object],[object Object],[object Object],[object Object]
Detecting the Probe-Target Hybrids ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Southern Blot
Cloning a Gene by Hybridization :   ,[object Object],[object Object],[object Object],[object Object],[object Object]
Constructing a Genomic Library ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Genomic Libraries
Plasmid Biotechnology
Polymerase Chain Reaction ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Polymerase Action ,[object Object],[object Object],[object Object]
Elements of a PCR ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Temperature Dependent Steps ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
The PCR Process ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
A Polymerase Chain Reaction
DNA Fingerprinting ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
RFLP Allele Analysis
Steps in DNA Fingerprinting ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Steps in DNA Fingerprinting, Cont . ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Uses of DNA Fingerprints ,[object Object],[object Object],[object Object],[object Object]
 
Diagnosis of Inherited Disorders ,[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object]
Developing Cures for Inherited Disorders   ,[object Object],[object Object],[object Object]
Personal Identification ,[object Object],[object Object],[object Object]

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