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Old man Saltbush Improvement
                   p




        Presented
           by
        Ram Nair
Background
              Southern Australia’s agricultural regions are
             experiencing a growing trend of variable rainfall and
             increasing risk of crop failure particularly in the more
             vulnerable marginal areas.

              Most
              M t common strategic response t managing climate
                               t t i               to        i li t
             variability in lower rainfall cropping areas is
             diversification and maintaining a healthy emphasis
             on livestock enterprises.

              Increasing prominence of livestock industries creates
                       gp
             opportunities for shrub forage species.

              Old man Saltbush (Atriplex nummularia) is a key
             species in productive and resilient grazing systems
             based on perennials.
Cultivar Development



       Development of clonal cultivar
OMS is native to Australia and grows naturally in harsh, arid areas. Seed
was collected from 600 plants at 27 localities (provenances) around
Australia. Comprehensive germplasm collections of OMS were established
in SA (Monarto), Condobolin (NSW) and Tammin (WA). - 20,000 plants at
each site. Sequential selection was conducted for key traits and elite
     site
plants identified for clonal evaluation trials.


       Development of seed cultivar
Suitable male and female plants identified from the in-situ collections will
be utilised for this.
Over 80 000 plants screened at 4 locations
            p
              2 subspecies
        Originating from 27 sites
Methodology




  Assessment of variability in:

          productivity – edible biomass (Adelaide method)

          animal preference (sheep grazing)
            i l     f       ( h        i )

          re-growth after grazing

          Nutritive value
Saltbush evaluation site at Monarto, SA
Saltbush evaluation site at Condobolin, NSW
Saltbush evaluation site at Tammin, NSW
Results:



    Significant variation was observed for edible biomass.

    Heritability for dibl bi
    H it bilit f edible biomass: 0.15 to 0.21
                                 0 15 t 0 21
What do the animals have to say?
Grazing plan at Monarto, SA
                                            Monarto




                                       25 /ha; 1.8 ha; therefore 45 sheep
           ( Low (rep 1)


        ( High (rep 1)                 50 /ha; 1.8 ha; therefore 90 sheep


            ( Low (rep 2)
                                       25 /ha; 1.8 ha; therefore 45 sheep


                    ( High (rep 2)       50 /ha; 0.9 ha; therefore 45sheep




Pre-conditioning paddocks
Grazing preference scoring
Score 1              Score 2                     Score 3




Score 4                                Score 5
Saltbush evaluation site at Monarto, SA
    - grazing preference by sheep
Saltbush evaluation site at Condobolin, NSW
       - grazing preference by sheep
Saltbush evaluation site at Tammin, NSW
- grazing preference by sheep
Results:



           A. nummularia ssp nummularia was grazed in preference
            to A. nummularia ssp spathulata at all sites.

       Heritability for grazing preference: 0.26 to 0.62
Results:




        Breeding values were calculated for each individual
                 shrub (20,000 plants at each site) for both
       edible biomass and grazing preference for use in index
        selection.

        Based on a selection index for edible biomass and
       grazing preference by sheep, 1000 plants were short-
                                                       short
       listed at each site.
Selections within A. nummularia

                                    selection index
                       1 Edible biomass + 1.5 Grazing preference


                  50
                  45
                  40
% Predicte gain




                  35
         ed




                  30
                                                                   Edible biomass
                  25
                  20                                               Grazing preference
                  15
                  10
                   5
                   0
                         Monarto    Condobolin     Tammin
Saltbush evaluation site at Tammin, NSW –
          regrowth after grazing
Results:



            Based on re-growth after grazing 100 plants were
           selected at each site for nutritive value analysis
           - (April 2009).
Nutritive value is the key selection factor




                   (Marta Monjardino, 2008)
Predicting organic matter digestibility of shrubs
                 (NIR method)
Predicted Organic Matter Digestibility



                    55.0

                    54.0

                    53.0
Predicted OMD (%)




                    52.0
          O




                    51.0

                    50.0
P




                    49.0

                    48.0

                    47.0
                    47 0
                           Tammin across all   Tammin EGG   Tammin top 10
                                lines
Predicted Organic Matter Digestibility



                    56.0

                    54.0

                    52.0
Predicted OMD (%)




                    50.0
          O




                    48.0

                    46.0
P




                    44.0

                    42.0

                    40.0
                           Condo across all   Condo EGG   Condo top 10
                                lines
Predicted Organic Matter Digestibility

                   58.0

                   57.0
              %)
                   56.0
                   56 0
Predicte OMD (%



                   55.0
       ed




                   54.0

                   53.0

                   52.0

                   51.0
                          Monarto across all   Monarto EGG   Monarto top 10
                                lines
Soluble Ash (% of DM)


 Tammin EGG


Tammin top 10

 Monarto EGG


Monarto top 10

  Condo EGG


 Condo top 10

                 0.0   5.0   10.0   15.0   20.0   25.0   30.0
89 elite plants were selected for clonal propagation -
(May June 2009).
(May-June 2009)
Clonal propagation


                                %
                                striking
                                rate of
                                cuttings
                 Range                     Mean

Monarto          8.93 70.34
                 8 93 – 70 34              31.28
                                           31 28

Condobolin       7.69 – 82.41              34.17

Tammin           9.59 – 85.80              46.91

EGG control      69.80

A. spathulatha   8.02
control
Clonal evaluation trial



         Replicated trials of the 89 selected clones and the two
         controls (EGG + spath) were planted at Monarto
         (SA), Dareton (NSW) and Tammin (WA) in spring 2009.

         Design
         D i – row column – At each site 2184 plants (91 8 x 3)
                     l            h it         l t (91x

         Data on dry matter production, animal preference and
         nutritive value will be collected. These trials will help to
         identify the best clone(s) for potential commercial release as
         cultivar(s) by 2011.
                 ( ) y
Clonal trial at Monarto, SA
Clonal trial at Dareton, NSW
Seed Cultivar development

Old man saltbush plant is dioecious (female and male plants).

Hybridisation nurseries are being established in SA.
                                                  SA
Hybrid lines will be developed for testing with the direct-
seeding method (by 2012).
Industry use Plan - Market Potential
            y
The market potential for Old man saltbush in Australia and overseas is
extensive.

However, two major impediments to the uptake of new saltbush plantings
in Australia are: 1) cost of seedlings and
                  2) the risk of unsuccessful establishment.
                                              establishment

Reduced planting incentives from the government may also limit uptake.
(Su ey
(Survey by Dr Anna Dutkiewicz, Adoption Manager FFI C C)
                  a ut e c , dopt o       a age     CRC)


Currently the major plantings of saltbush is by seedlings.


Cost of planting saltbush seedlings can vary between $650 to 850 per ha,
in contrast direct seeding can cost between $150 to 185 per ha
                                                            ha.
Acknowledgements:


Hayley Norman, CSIRO
Allan Rintoul, CSIRO
Peter Jessop, NSW DPI
             p,
Eric Bailey, NSW DPI
Merv Tucker, DWLBC
Glenn Gale, DWLBC
                            Richard Mazanec, DEC; WA
Ed Barrett-Lennard, DAFWA   Mike Bennell, formerly DWLBC
Joe Jacobs, DPI Vic
    Jacobs                  David McKenna, formerly DWLBC
                                  McKenna

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Improving saltbush

  • 1. Old man Saltbush Improvement p Presented by Ram Nair
  • 2. Background Southern Australia’s agricultural regions are experiencing a growing trend of variable rainfall and increasing risk of crop failure particularly in the more vulnerable marginal areas. Most M t common strategic response t managing climate t t i to i li t variability in lower rainfall cropping areas is diversification and maintaining a healthy emphasis on livestock enterprises. Increasing prominence of livestock industries creates gp opportunities for shrub forage species. Old man Saltbush (Atriplex nummularia) is a key species in productive and resilient grazing systems based on perennials.
  • 3. Cultivar Development Development of clonal cultivar OMS is native to Australia and grows naturally in harsh, arid areas. Seed was collected from 600 plants at 27 localities (provenances) around Australia. Comprehensive germplasm collections of OMS were established in SA (Monarto), Condobolin (NSW) and Tammin (WA). - 20,000 plants at each site. Sequential selection was conducted for key traits and elite site plants identified for clonal evaluation trials. Development of seed cultivar Suitable male and female plants identified from the in-situ collections will be utilised for this.
  • 4. Over 80 000 plants screened at 4 locations p 2 subspecies Originating from 27 sites
  • 5. Methodology Assessment of variability in: productivity – edible biomass (Adelaide method) animal preference (sheep grazing) i l f ( h i ) re-growth after grazing Nutritive value
  • 6. Saltbush evaluation site at Monarto, SA
  • 7. Saltbush evaluation site at Condobolin, NSW
  • 8. Saltbush evaluation site at Tammin, NSW
  • 9. Results: Significant variation was observed for edible biomass. Heritability for dibl bi H it bilit f edible biomass: 0.15 to 0.21 0 15 t 0 21
  • 10. What do the animals have to say?
  • 11. Grazing plan at Monarto, SA Monarto 25 /ha; 1.8 ha; therefore 45 sheep ( Low (rep 1) ( High (rep 1) 50 /ha; 1.8 ha; therefore 90 sheep ( Low (rep 2) 25 /ha; 1.8 ha; therefore 45 sheep ( High (rep 2) 50 /ha; 0.9 ha; therefore 45sheep Pre-conditioning paddocks
  • 12. Grazing preference scoring Score 1 Score 2 Score 3 Score 4 Score 5
  • 13. Saltbush evaluation site at Monarto, SA - grazing preference by sheep
  • 14. Saltbush evaluation site at Condobolin, NSW - grazing preference by sheep
  • 15. Saltbush evaluation site at Tammin, NSW - grazing preference by sheep
  • 16. Results: A. nummularia ssp nummularia was grazed in preference to A. nummularia ssp spathulata at all sites. Heritability for grazing preference: 0.26 to 0.62
  • 17. Results: Breeding values were calculated for each individual shrub (20,000 plants at each site) for both edible biomass and grazing preference for use in index selection. Based on a selection index for edible biomass and grazing preference by sheep, 1000 plants were short- short listed at each site.
  • 18. Selections within A. nummularia selection index 1 Edible biomass + 1.5 Grazing preference 50 45 40 % Predicte gain 35 ed 30 Edible biomass 25 20 Grazing preference 15 10 5 0 Monarto Condobolin Tammin
  • 19. Saltbush evaluation site at Tammin, NSW – regrowth after grazing
  • 20. Results: Based on re-growth after grazing 100 plants were selected at each site for nutritive value analysis - (April 2009).
  • 21. Nutritive value is the key selection factor (Marta Monjardino, 2008)
  • 22. Predicting organic matter digestibility of shrubs (NIR method)
  • 23. Predicted Organic Matter Digestibility 55.0 54.0 53.0 Predicted OMD (%) 52.0 O 51.0 50.0 P 49.0 48.0 47.0 47 0 Tammin across all Tammin EGG Tammin top 10 lines
  • 24. Predicted Organic Matter Digestibility 56.0 54.0 52.0 Predicted OMD (%) 50.0 O 48.0 46.0 P 44.0 42.0 40.0 Condo across all Condo EGG Condo top 10 lines
  • 25. Predicted Organic Matter Digestibility 58.0 57.0 %) 56.0 56 0 Predicte OMD (% 55.0 ed 54.0 53.0 52.0 51.0 Monarto across all Monarto EGG Monarto top 10 lines
  • 26. Soluble Ash (% of DM) Tammin EGG Tammin top 10 Monarto EGG Monarto top 10 Condo EGG Condo top 10 0.0 5.0 10.0 15.0 20.0 25.0 30.0
  • 27. 89 elite plants were selected for clonal propagation - (May June 2009). (May-June 2009)
  • 28. Clonal propagation % striking rate of cuttings Range Mean Monarto 8.93 70.34 8 93 – 70 34 31.28 31 28 Condobolin 7.69 – 82.41 34.17 Tammin 9.59 – 85.80 46.91 EGG control 69.80 A. spathulatha 8.02 control
  • 29. Clonal evaluation trial Replicated trials of the 89 selected clones and the two controls (EGG + spath) were planted at Monarto (SA), Dareton (NSW) and Tammin (WA) in spring 2009. Design D i – row column – At each site 2184 plants (91 8 x 3) l h it l t (91x Data on dry matter production, animal preference and nutritive value will be collected. These trials will help to identify the best clone(s) for potential commercial release as cultivar(s) by 2011. ( ) y
  • 30. Clonal trial at Monarto, SA
  • 31. Clonal trial at Dareton, NSW
  • 32. Seed Cultivar development Old man saltbush plant is dioecious (female and male plants). Hybridisation nurseries are being established in SA. SA Hybrid lines will be developed for testing with the direct- seeding method (by 2012).
  • 33. Industry use Plan - Market Potential y The market potential for Old man saltbush in Australia and overseas is extensive. However, two major impediments to the uptake of new saltbush plantings in Australia are: 1) cost of seedlings and 2) the risk of unsuccessful establishment. establishment Reduced planting incentives from the government may also limit uptake. (Su ey (Survey by Dr Anna Dutkiewicz, Adoption Manager FFI C C) a ut e c , dopt o a age CRC) Currently the major plantings of saltbush is by seedlings. Cost of planting saltbush seedlings can vary between $650 to 850 per ha, in contrast direct seeding can cost between $150 to 185 per ha ha.
  • 34. Acknowledgements: Hayley Norman, CSIRO Allan Rintoul, CSIRO Peter Jessop, NSW DPI p, Eric Bailey, NSW DPI Merv Tucker, DWLBC Glenn Gale, DWLBC Richard Mazanec, DEC; WA Ed Barrett-Lennard, DAFWA Mike Bennell, formerly DWLBC Joe Jacobs, DPI Vic Jacobs David McKenna, formerly DWLBC McKenna