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DuPont Food Innovations




 > The Challenge           > The Solution              > Cases                    > Why DuPont




             The BAX® System
             from DuPont Qualicon
             Pathogen detection the easy way

             Testing for pathogens is vital to the food industry, but traditional culture methods can
             be time-consuming and require highly skilled technicians for accurate interpretation.
             Newer antibody-based methods are faster but may not detect low concentrations, or
             they can cross-react with related bacteria to produce false positive results, which
             lead to re-testing that, can dramatically slow time to market.

             The BAX System from DuPont Qualicon, however, uses advanced molecular science to
                     ®



             focus on the genetic structure of the target microbe. This automated DNA-based
             method combines speed and ease of use with unprecedented performance to reliably
             detect pathogens, such as Salmonella and E. coli O157:H7, in food and environmental
             samples. Contact us to learn how the BAX System can help streamline your testing
                                                       ®



             procedures and save you money.




http://food.dupont.com                                                                                  Page 1
DuPont Food Innovations



                                         New challenges in a global world

> The Challenge
> The Solution
> Cases
> Why DuPont




                                         Food processors need accurate results from pathogen tests of their raw materials,
  Contact Us                             environmental samples and end products to help ensure the food they release to the
                                         market is safe. While accuracy is a top priority to avoid recalls and protect the brand,
 Call our food experts*                  a processing plant in this fast-paced global food system cannot afford to use slow
 Tel. +800 387 668 38
  or +34 985 12 37 73                    or unreliable testing methods if it wants to be efficient and profitable. Fortunately,
                                         DNA-based testing systems, such as the BAX System, offer advanced science, including
                                                                                     ®

  or send us your questions
  http://dupont.com/food-contact
                                         the power of the polymerase chain reaction (PCR) to rapidly amplify a genetic fragment
                                         of the targeted pathogen to detectable levels within hours instead of days or weeks.



  More Information about
  DuPont solutions for
  pathogen detection on

  http://dupont.com/pathogen-detection




http://food.dupont.com                                                                                                     Page 2
DuPont Food Innovations



                                         Pathogen testing: Accurate and Fast

> The Challenge
> The Solution
> Cases
> Why DuPont




                                         The award-winning BAX System is a fast and accurate method for detecting
                                                                ®




  Contact Us                             pathogens or other unwelcome organisms in a broad range of food and environmental
                                         samples. The system breaks down samples at the genetic level, using the power of
 Call our food experts*                  PCR to detect the target microbe with certainty.
 Tel. +800 387 668 38
  or +34 985 12 37 73
                                         Advanced technology with the BAX System
                                                                             ®


  or send us your questions
  http://dupont.com/food-contact
                                         Beyond accurate results for presence/absence, the BAX System offers advanced
                                                                                                ®



                                         capabilities for multiple target detection in the same well. Probe-based chemistry can
                                         provide for additional information, such as species differentiation within each sample.
                                         With a single program for several bacteria, the BAX System allows you to test
                                                                                               ®

  More Information about
  DuPont solutions for                   multiple targets in the same run, up to 96 samples per batch. Tabletted PCR reagents,
  pathogen detection on                  which enable minimal hands-on time using standard laboratory techniques, provide
  http://dupont.com/pathogen-detection   for long shelf life and consistency.
                                         Friendly screen prompts guide you through the entire procedure, reducing the need
                                         for highly skilled technicians and expensive training. Depending on the test chemistry,
                                         the system delivers results on prepared samples within 1 to 3.5 hours of processing
                                         time. These results are clearly displayed on screen with a simple positive or negative
                                         icon that eliminates the need for expert interpretation. Electronic files allow you to
                                         print, share and store your results for easy archiving and retrieval.
                                         Customers around the world have made the BAX System an integral part of their
                                                                                           ®



                                         quality control systems due to its tremendous capability for impact on their
                                         operations--from dramatically decreasing false positives and minimizing re-testing to
                                         reducing employee training and speeding the time to market.
                                         The speed and accuracy of DNA-based detection. The simplicity of a load-it-and-leave-it
                                         operation. The familiarity of a Microsoft Windows interface. The BAX system
                                                                                  ®          ®                   ®



                                         combines it all for smooth testing procedures at any stage in the processing pipeline




http://food.dupont.com                                                                                                    Page 3
DuPont Food Innovations



                                         Salmonella detection
                                         with the BAX® System
> The Challenge
> The Solution
> Cases
> Why DuPont




                                         When Salmonella are present in a food or environmental sample, the genetic-based
                                         BAX System uses the power of the polymerase chain reaction (PCR) to rapidly create
                                            ®

  Contact Us
                                         (amplify) millions of copies of a unique, Salmonella-specific DNA fragment for reliable
 Call our food experts*                  Salmonella detection.
 Tel. +800 387 668 38
  or +34 985 12 37 73
                                         Advantages of the BAX System:
                                                               ®


  or send us your questions
  http://dupont.com/food-contact
                                         - Single tablet that combines all of the PCR reagents you need for Salmonella
                                           detection
                                         - Minimal hands-on time, in addition to accuracy, long shelf life and consistency
  More Information about
                                         - Automated processing in 1 to 3.5 hours for a full rack of 96 tests
  DuPont solutions for                   - Data can be easily stored, shared or printed
  pathogen detection on

  http://dupont.com/pathogen-detection



                                         E. coli O157:H7 testing
                                         with BAX® System
                                         Developed in collaboration with the USDA Agricultural Research Service and
                                         validated on real-world sample sizes, this extremely reliable and highly accurate
                                         assay incorporates Scorpion probes for advanced real-time PCR technology. This
                                                                     ®



                                         assay delivers superior sensitivity and specificity so you can detect all known
                                         E. coli O157:H7 –- even atypical isolates.




http://food.dupont.com                                                                                                       Page 4
DuPont Food Innovations



                                         Pathogen testing case studies
                                         Choosing the best tool for pathogen detection doesn't
                                         have to be difficult
> The Challenge
                                         Every food company wants a pathogen detection method that is fast, reliable and
> The Solution
                                         easy to use. The quality control labs understand that PCR-based testing systems offer
> Cases                                  advanced science, with results that are faster than culture and more accurate than
                                         antibodies. But how are they to know which PCR system is best? For one company, the
> Why DuPont
                                         choice was easy.

                                         Salmonella testing: It‘s not just for the plant anymore

                                         When Salmonella shows up in the plant, food companies need accurate testing
  Contact Us
                                         methods to verify that their interventions are working. The BAX System allowed this
                                                                                                          ®




 Call our food experts*                  poultry processor to do just that, plus extend their monitoring into feed, flocks and
 Tel. +800 387 668 38                    other supply areas.
  or +34 985 12 37 73

  or send us your questions
                                         Reducing false positives is critical for processed meat producer
  http://dupont.com/food-contact

                                         Although this producer of processed meat products implemented good measures to
                                         significantly reduce the risk of pathogens, its quality assurance testing method
                                         consistently reported false positives. Incorporating the BAX System into routine
                                                                                                       ®


  More Information about
  DuPont solutions for
                                         testing has made a tremendous difference.
  pathogen detection on

  http://dupont.com/pathogen-detection




                                           Salmonella Testing and Detection
                                           Salmonella is one of the most common causes of food-borne illness, with
                                           about 140,000 reported cases of Salmonellosis annually in the European
                                           Union. Various serotypes have been associated with meat, poultry, eggs, milk,
                                           fish, sauces, cream-filled desserts, peanut butter, chocolate and other foods.

                                           With an increase in antibiotic resistance and an upward prevalence trend in
                                           broilers, food processors need fast and accurate testing methods. Traditional
                                           isolation from culture requires many steps and more than four days to get
                                           results.




http://food.dupont.com                                                                                                    Page 5
DuPont Food Innovations



                                                 Why DuPont?

> The Challenge
> The Solution
> Cases
> Why DuPont




                                                 DuPont is a science company. Founded in 1802, DuPont puts science to work by
   Contact Us                                    solving problems and creating solutions that make people’s lives better, safer and
                                                 easier. Operating in more than 70 countries, the company offers a wide range of
   Call our food experts*                        products and services to markets including agriculture, nutrition, electronics,
   Tel. +800 387 668 38                          communications, safety and protection, home and construction, transportation and
   or +34 985 12 37 73
                                                 apparel.
   or send us your questions
   http://dupont.com/food-contact




                                                 Contact us
   More Information about
   DuPont solutions for                          If you have any questions or you want to learn more about DuPont pathogen detection
   pathogen detection on                         solutions, please contact us:
   http://dupont.com/pathogen-detection
                                                 Call our Food Industry Experts on
                                                 Tel. +800 387 668 68
                                                 or +34 985 12 37 73
*Toll free number in CH, FR, DE, IT, UK, ES,
DK, SE, NO, BE, FI, LU, GR, PT, AT, IE when
calling from a landline. Mobile phone calls      Send us your question on
may or may not be toll free depending on
provider. Language options in English, French,   www2.dupont.com/Food__Innovations/en_GB/contact/index.html?src=fdemea_uk_doc_saf
German and Italian. All others please use
contact form.

                                                 or read more on
                                                 www2.dupont.com/Food__Innovations/en_GB/food-safety/pathogen-detection.html?src=fdemea_uk_doc_saf




http://food.dupont.com                                                                                                                                                                  Page 6

© 2011 DuPont. The DuPont Oval Logo, DuPont™, and the miracles of science™ are trademarks or registered trademarks of E.I. du Pont de Nemours and Company or its affiliates. All rights reserved.

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Pathogen Detecion

  • 1. DuPont Food Innovations > The Challenge > The Solution > Cases > Why DuPont The BAX® System from DuPont Qualicon Pathogen detection the easy way Testing for pathogens is vital to the food industry, but traditional culture methods can be time-consuming and require highly skilled technicians for accurate interpretation. Newer antibody-based methods are faster but may not detect low concentrations, or they can cross-react with related bacteria to produce false positive results, which lead to re-testing that, can dramatically slow time to market. The BAX System from DuPont Qualicon, however, uses advanced molecular science to ® focus on the genetic structure of the target microbe. This automated DNA-based method combines speed and ease of use with unprecedented performance to reliably detect pathogens, such as Salmonella and E. coli O157:H7, in food and environmental samples. Contact us to learn how the BAX System can help streamline your testing ® procedures and save you money. http://food.dupont.com Page 1
  • 2. DuPont Food Innovations New challenges in a global world > The Challenge > The Solution > Cases > Why DuPont Food processors need accurate results from pathogen tests of their raw materials, Contact Us environmental samples and end products to help ensure the food they release to the market is safe. While accuracy is a top priority to avoid recalls and protect the brand, Call our food experts* a processing plant in this fast-paced global food system cannot afford to use slow Tel. +800 387 668 38 or +34 985 12 37 73 or unreliable testing methods if it wants to be efficient and profitable. Fortunately, DNA-based testing systems, such as the BAX System, offer advanced science, including ® or send us your questions http://dupont.com/food-contact the power of the polymerase chain reaction (PCR) to rapidly amplify a genetic fragment of the targeted pathogen to detectable levels within hours instead of days or weeks. More Information about DuPont solutions for pathogen detection on http://dupont.com/pathogen-detection http://food.dupont.com Page 2
  • 3. DuPont Food Innovations Pathogen testing: Accurate and Fast > The Challenge > The Solution > Cases > Why DuPont The award-winning BAX System is a fast and accurate method for detecting ® Contact Us pathogens or other unwelcome organisms in a broad range of food and environmental samples. The system breaks down samples at the genetic level, using the power of Call our food experts* PCR to detect the target microbe with certainty. Tel. +800 387 668 38 or +34 985 12 37 73 Advanced technology with the BAX System ® or send us your questions http://dupont.com/food-contact Beyond accurate results for presence/absence, the BAX System offers advanced ® capabilities for multiple target detection in the same well. Probe-based chemistry can provide for additional information, such as species differentiation within each sample. With a single program for several bacteria, the BAX System allows you to test ® More Information about DuPont solutions for multiple targets in the same run, up to 96 samples per batch. Tabletted PCR reagents, pathogen detection on which enable minimal hands-on time using standard laboratory techniques, provide http://dupont.com/pathogen-detection for long shelf life and consistency. Friendly screen prompts guide you through the entire procedure, reducing the need for highly skilled technicians and expensive training. Depending on the test chemistry, the system delivers results on prepared samples within 1 to 3.5 hours of processing time. These results are clearly displayed on screen with a simple positive or negative icon that eliminates the need for expert interpretation. Electronic files allow you to print, share and store your results for easy archiving and retrieval. Customers around the world have made the BAX System an integral part of their ® quality control systems due to its tremendous capability for impact on their operations--from dramatically decreasing false positives and minimizing re-testing to reducing employee training and speeding the time to market. The speed and accuracy of DNA-based detection. The simplicity of a load-it-and-leave-it operation. The familiarity of a Microsoft Windows interface. The BAX system ® ® ® combines it all for smooth testing procedures at any stage in the processing pipeline http://food.dupont.com Page 3
  • 4. DuPont Food Innovations Salmonella detection with the BAX® System > The Challenge > The Solution > Cases > Why DuPont When Salmonella are present in a food or environmental sample, the genetic-based BAX System uses the power of the polymerase chain reaction (PCR) to rapidly create ® Contact Us (amplify) millions of copies of a unique, Salmonella-specific DNA fragment for reliable Call our food experts* Salmonella detection. Tel. +800 387 668 38 or +34 985 12 37 73 Advantages of the BAX System: ® or send us your questions http://dupont.com/food-contact - Single tablet that combines all of the PCR reagents you need for Salmonella detection - Minimal hands-on time, in addition to accuracy, long shelf life and consistency More Information about - Automated processing in 1 to 3.5 hours for a full rack of 96 tests DuPont solutions for - Data can be easily stored, shared or printed pathogen detection on http://dupont.com/pathogen-detection E. coli O157:H7 testing with BAX® System Developed in collaboration with the USDA Agricultural Research Service and validated on real-world sample sizes, this extremely reliable and highly accurate assay incorporates Scorpion probes for advanced real-time PCR technology. This ® assay delivers superior sensitivity and specificity so you can detect all known E. coli O157:H7 –- even atypical isolates. http://food.dupont.com Page 4
  • 5. DuPont Food Innovations Pathogen testing case studies Choosing the best tool for pathogen detection doesn't have to be difficult > The Challenge Every food company wants a pathogen detection method that is fast, reliable and > The Solution easy to use. The quality control labs understand that PCR-based testing systems offer > Cases advanced science, with results that are faster than culture and more accurate than antibodies. But how are they to know which PCR system is best? For one company, the > Why DuPont choice was easy. Salmonella testing: It‘s not just for the plant anymore When Salmonella shows up in the plant, food companies need accurate testing Contact Us methods to verify that their interventions are working. The BAX System allowed this ® Call our food experts* poultry processor to do just that, plus extend their monitoring into feed, flocks and Tel. +800 387 668 38 other supply areas. or +34 985 12 37 73 or send us your questions Reducing false positives is critical for processed meat producer http://dupont.com/food-contact Although this producer of processed meat products implemented good measures to significantly reduce the risk of pathogens, its quality assurance testing method consistently reported false positives. Incorporating the BAX System into routine ® More Information about DuPont solutions for testing has made a tremendous difference. pathogen detection on http://dupont.com/pathogen-detection Salmonella Testing and Detection Salmonella is one of the most common causes of food-borne illness, with about 140,000 reported cases of Salmonellosis annually in the European Union. Various serotypes have been associated with meat, poultry, eggs, milk, fish, sauces, cream-filled desserts, peanut butter, chocolate and other foods. With an increase in antibiotic resistance and an upward prevalence trend in broilers, food processors need fast and accurate testing methods. Traditional isolation from culture requires many steps and more than four days to get results. http://food.dupont.com Page 5
  • 6. DuPont Food Innovations Why DuPont? > The Challenge > The Solution > Cases > Why DuPont DuPont is a science company. Founded in 1802, DuPont puts science to work by Contact Us solving problems and creating solutions that make people’s lives better, safer and easier. Operating in more than 70 countries, the company offers a wide range of Call our food experts* products and services to markets including agriculture, nutrition, electronics, Tel. +800 387 668 38 communications, safety and protection, home and construction, transportation and or +34 985 12 37 73 apparel. or send us your questions http://dupont.com/food-contact Contact us More Information about DuPont solutions for If you have any questions or you want to learn more about DuPont pathogen detection pathogen detection on solutions, please contact us: http://dupont.com/pathogen-detection Call our Food Industry Experts on Tel. +800 387 668 68 or +34 985 12 37 73 *Toll free number in CH, FR, DE, IT, UK, ES, DK, SE, NO, BE, FI, LU, GR, PT, AT, IE when calling from a landline. Mobile phone calls Send us your question on may or may not be toll free depending on provider. Language options in English, French, www2.dupont.com/Food__Innovations/en_GB/contact/index.html?src=fdemea_uk_doc_saf German and Italian. All others please use contact form. or read more on www2.dupont.com/Food__Innovations/en_GB/food-safety/pathogen-detection.html?src=fdemea_uk_doc_saf http://food.dupont.com Page 6 © 2011 DuPont. The DuPont Oval Logo, DuPont™, and the miracles of science™ are trademarks or registered trademarks of E.I. du Pont de Nemours and Company or its affiliates. All rights reserved.