International Journal of Engineering Research and Development
Bioceramics in Simulated Body Fluid
1. 1 23
Journal of Materials Science:
Materials in Medicine
Official Journal of the European Society
for Biomaterials
ISSN 0957-4530
J Mater Sci: Mater Med
DOI 10.1007/s10856-014-5229-x
Investigating the surface reactivity of SiO2–
TiO2–CaO–Na2O/SrO bioceramics as a
function of structure and incubation time
in simulated body fluid
Y. Li, A. Coughlan & Anthony. W. Wren
2. 1 23
Your article is protected by copyright and all
rights are held exclusively by Springer Science
+Business Media New York. This e-offprint is
for personal use only and shall not be self-
archived in electronic repositories. If you wish
to self-archive your article, please use the
accepted manuscript version for posting on
your own website. You may further deposit
the accepted manuscript version in any
repository, provided it is only made publicly
available 12 months after official publication
or later and provided acknowledgement is
given to the original source of publication
and a link is inserted to the published article
on Springer's website. The link must be
accompanied by the following text: "The final
publication is available at link.springer.com”.
3. Investigating the surface reactivity of SiO2–TiO2–CaO–Na2O/SrO
bioceramics as a function of structure and incubation time
in simulated body fluid
Y. Li • A. Coughlan • Anthony. W. Wren
Received: 21 January 2014 / Accepted: 21 April 2014
Ó Springer Science+Business Media New York 2014
Abstract This study focuses on evaluating the biocom-
patibility of a SiO2–TiO2–CaO–Na2O/SrO glass and glass–
ceramic series. Glass and ceramic samples were synthe-
sized and characterized using X-ray diffraction. Each
material was subject to maturation in simulated body fluid
over 1, 7 and 30 days to describe any changes in surface
morphology. Calcium phosphate (CaP) deposition was
observed predominantly on the Na?
containing amorphous
and crystalline materials, with plate-like morphology. The
precipitated surface layer was also observed to crystallize
with respect to maturation, which was most evident in the
amorphous Na?
containing glasses, Ly-N and Ly-C. The
addition of Sr2?
greatly reduced the solubility of all sam-
ples, with limited CaP precipitation on the amorphous
samples and no deposition on the crystalline materials. The
morphology of the samples was also different, presenting
irregular plate-like structures (Ly-N), needle-like deposits
(Ly-C) and globular-like structures (Ly-S). Cell culture
analysis presented a significant increase in cell viability
with the Na?
materials, 134 %, while the Sr2?
containing
glasses, 60–80 % and ceramics, 60–85 % presented a
general reduction in cell viability, however these reduc-
tions were not significant.
1 Introduction
In recent years bioactive glasses and ceramics have stim-
ulated interest as materials that can stimulate the regener-
ation of bone tissue [1]. A common and widely studied
characteristic of bioactive glasses and glass–ceramics if the
formation of a biologically active apatite (A) layer which
supports bone bonding which can be evaluated using
simulated body fluid (SBF), which is a solution that con-
tains an ionic composition similar to that of human blood
plasma [2, 3]. Prior to the 1970s, artificial materials that
were implanted into the human body, specifically bone
defects, resulted in the materials being encapsulated by
fibrous tissue which resulted in the materials isolation for
the surrounding bone. In the early 1970s, Hench [4, 5]
produced glass in the Na2O–CaO–SiO2–P2O5 system that
spontaneously bonds to living bone without the formation
of surrounding fibrous tissue. Since this development,
many different types of ceramics such as sintered
hydroxyapatite, sintered b-tricalcium phosphate, A/b-tri-
calcium phosphate biphasic ceramics and glass/ceramic A–
W (wollastonite) have been shown to bond to living bone
[2].
Additionally, many different materials have been pro-
duced from bioactive glass and ceramics that can be used
for numerous medical applications. These materials include
glass–ceramic scaffolds [6–8] for bone repair, glass
microspheres for cancer treatment [9–11], composite
materials for drug release [12, 13] and also composite
materials where bioactive glasses are used to improve
bioactivity or mechanical strength [14–17]. Bioactive glass
and ceramics are a valuable addition to medical materials
as they can incorporate biological compatibility with
mechanical strength and bone adhesion, vital components
for skeletal repair [18]. However, uncertainties still exists
Y. Li Á Anthony. W. Wren (&)
Inamori School of Engineering, Alfred University, Alfred,
NY 14802, USA
e-mail: wren@alfred.edu
A. Coughlan
School of Materials Engineering, Purdue University,
West Lafayette, IN, USA
123
J Mater Sci: Mater Med
DOI 10.1007/s10856-014-5229-x
Author's personal copy
4. relating to the mechanical durability due to their interac-
tions within the biological environment, and also the effect
of mechanical strain and the dissolution rate of materials,
and as such, altering the mechanical properties of a bio-
active glass without compromising its bioactivity is of key
interest [18]. This concern has previously been highlighted
when producing glass–ceramic scaffolds from 45S5 Bio-
glassÒ
, where it has been suggested that crystallization of
45S5 BioglassÒ
can reduce its bioactivity as, post sintering,
crystallization turns the material from being a bioactive
material into an inert ceramic [19]. However, there have
since been studies that suggest the predominant crystal
phase of 45S5 BioglassÒ
(Na2Ca2Si3O9), can significantly
improve the mechanical properties of the material, and that
crystallization does not inhibit the bioactivity as precipi-
tation of an amorphous calcium phosphate (CaP) occurs in
biological fluids [20–22].
This study was conducted to determine any differences
in bioactivity and subsequent changes in surface mor-
phology, specifically in SBF and cell culture, as a function
of material composition (Na?
/Sr2?
concentration), incu-
bation time, and structure (amorphous/crystalline). The
starting glass composition, SiO2–TiO2–CaO–Na2O/SrO,
alters Na?
and Sr2?
concentration which will affect the
dissolution rate of the glasses. A previous study by the
authors on the solubility of these materials determined that
(1) crystallization greatly reduces the ion release rate, (2)
pH is also reduced with crystallization, (3) the mechanical
durability of the materials is greatly enhanced by crystal-
lization where no significant changes are observed after
30 days in aqueous media and (4) the Na?
containing
materials produces higher ion release rates than the Sr2?
analogues. The glass composition utilized in this study was
selected as Na?
is critical in promoting dissolution of the
glass as it acts as a network modifier within the glass
network [22, 23]. Sr2?
also acts as a network modifier
within the glass, however, it shares atomic similarities to
Ca2?
and has been previously investigated and applied to
treating postmenopausal women with osteoporosis [24, 25].
Titanium (Ti) has been incorporated as numerous studies
cite that Ti containing materials result in the deposition of
CaP surface layer when incubated in SBF [26]. That can be
attributed to the formation of Ti–OH groups providing
favourable conditions for Ca precipitation [26].
2 Materials and methods
2.1 Glass synthesis
Three glass compositions (Ly-N, Ly-C, Ly-S) were formu-
lated for this study with the principal aim being to inves-
tigate any property changes with the modification of
sodium (Na?
) and strontium (Sr2?
) in the glass. A control
glass (Ly-C) was also formulated which contained equal
quantities of Na?
and Sr2?
. Glasses were prepared by
weighing out appropriate amounts of analytical grade
reagents and ball milling (1 h, Table 1).
2.1.1 Glass powder production
The powdered mixes were oven dried (100 °C, 1 h) and
fired (1,500 °C, 1 h) in platinum crucibles and shock
quenched into water. The resulting frits were dried, ground
and sieved to retrieve glass powders with a maximum
particle size of 45 lm.
2.1.2 Disc sample preparation
Disc samples (Ly-N, Ly-C, Ly-S) were prepared by
weighing approximately 0.5 g powder into a stainless steel
die (sample dimensions 1.5 9 6/ mm) which was pressed
under 3 tonnes of pressure. Disc samples were kept
amorphous for Ly-N, Ly-C, Ly-S by heat treating the
pressed discs below the glass transition temperature for
24 h, and crystalline analogues were heat treated to the
sintering temperature for Ly-N (653 °C), Ly-C (713 °C)
and Ly-S (825 °C) in order to determine any differences in
bioactivity as a result of structure. Disc samples were then
used for SBF testing and while 100 ll extracts were used
for indirect cell culture analysis.
2.2 Glass characterization
2.2.1 X-ray diffraction (XRD)
Diffraction patterns were collected using a Siemens D5000
XRD Unit (Bruker AXS, Inc., WI, USA). Glass powder
samples were packed into standard stainless steel sample
holders. A generator voltage of 40 kV and a tube current of
30 mA was employed. Diffractograms were collected in
the range 10° 2h 80°, at a scan step size 0.02° and a
step time of 10 s. Any crystalline phases present were
identified using JCPDS (Joint Committee for Powder Dif-
fraction Studies) standard diffraction patterns (data repro-
duced from previously published manuscript [27], Fig. 1;
Table 3).
Table 1 Glass composition
(mol. fr.)
Ly-N Ly-C Ly-S
SiO2 0.55 0.55 0.55
TiO2 0.05 0.05 0.05
CaO 0.22 0.22 0.22
Na2O 0.18 0.09 0.00
SrO 0.00 0.09 0.18
J Mater Sci: Mater Med
123
Author's personal copy
5. 2.2.2 Hot stage microscopy (HSM)
A MISURA side view HSM, Expert Systems (Modena,
Italy), with image analysis system and electrical furnace,
with max temperature of 1,600 °C and max rate of 80 °C/
min. The parameters for this experiment were a heat rate of
20 °C/min from 20 to 500 °C and 5 °C/min from 500 to
1,200 °C. The computerized image analysis system auto-
matically records and analyses the sample geometry during
heating.
2.2.3 Scanning electron microscopy and energy dispersive
X-ray analysis (SEM–EDS)
Backscattered electron imaging was carried out with an
FEI Co. Quanta 200F Environmental SEM. Additional
compositional analysis was performed with an EDAX
Genesis Energy-Dispersive Spectrometer. All EDS spectra
were collected at 20 kV using a beam current of 26 nA.
Quantitative EDS spectra were subsequently converted into
relative concentration data.
Fig. 1 Scanning electron
microscopy of a Ly-N, b
Ly-C and c Ly-S including
corresponding EDX and
quantitative composition in
mol%
J Mater Sci: Mater Med
123
Author's personal copy
6. 2.3 Biocompatibility testing
2.3.1 Sample preparation
Discs of each glass were autoclaved prior to use and sterile
de-ionized water was used as the solvent to prepare
extracts. The volume of extract was determined using
Eq. 1. Vs is the volume of extract used, Sa is the exposed
surface area of the disc.
Vs ¼
Sa
10
: ð1Þ
Samples (n = 3 amorphous, and n = 3 crystalline) were
aseptically immersed in appropriate volumes of sterile de-
ionized water and agitated at (37 ± 2 °C) for 1, 7 and
30 days after which 100 ll of fluid was used for cytotox-
icity testing.
2.3.2 SBF trial
SBF was produced in accordance with the procedure out-
lined by Kokubo and Takadama [2]. The composition of
SBF is outlined in Table 2. The reagents were dissolved in
order, from reagent 1 to 9, in 500 ml of purified water
using a magnetic stirrer. The solution was maintained at
36.5 °C. 1 M-HCl was titrated to adjust the pH of the SBF
to 7.4. Purified water was then used to adjust the volume of
the solution up to 1 l. Glass/ceramic discs (n = 2) were
immersed in concentrations of SBF as determined by Eq. 1
and were subsequently stored in for 1, 7 and 30 days in an
incubator at 37 °C. A JEOL JSM-840 SEM equipped with
a Princeton Gamma Tech energy dispersive X-ray (EDX)
system was used to obtain secondary electron images and
carry out chemical analysis of the surface of glass and
ceramic discs. All EDX spectra were collected at 20 kV,
using a beam current of 0.26 nA. Quantitative EDX con-
verted the collected spectra into concentration data by
using standard reference spectra obtained from pure ele-
ments under similar operating parameters.
2.3.3 Cell culture analysis
The established cell line L929 (American Type Culture
Collection CCL 1 fibroblast, NCTC clone 929) was used in
this study as required by ISO10993 part 5. Cells were
maintained on a regular feeding regime in a cell culture
incubator at 37 °C/5 % CO2/95 % air atmosphere. Cells
were seeded into 24 well plates at a density of 10,000 cells
per well and incubated for 24 h prior to testing with both
extracts and cement discs. The culture media used was
M199 media supplemented with 10 % fetal bovine serum
(Sigma Aldrich, Ireland) and 1 % (2 mM) L-glutamine
(Sigma Aldrich, Ireland). The cytotoxicity of cement
extracts was evaluated using the methyl tetrazolium (MTT)
assay in 24 well plates. Aliquots (100 ll) of undiluted
sample were added into wells containing L929 cells in
culture medium (1 ml) in triplicate over 1, 7 and 30 days.
Cement discs (n = 3 amorphous, and n = 3 crystalline)
were placed in the plate wells and were tested after 24 h.
Each of the prepared plates was incubated for 24 h at
37 °C/5 % CO2. The MTT assay was then added in an
amount equal to 10 % of the culture medium volume/well.
The cultures were then re-incubated for a further 2 h
(37 °C/5 % CO2). Next, the cultures were removed from
the incubator and the resultant formazan crystals were
dissolved by adding an amount of MTT Solubilization
solution [10 % Triton X-100 in acidic isopropanol (0.1 N
HCI)] equal to the original culture medium volume. Once
the crystals were fully dissolved, the absorbance was
measured at a wavelength of 570 nm. Aliquots (100 ll) of
tissue culture water were used as controls, and cells were
assumed to have metabolic activities of 100 %.
2.4 Statistical analysis
One-way analysis of variance was employed to compare
the difference in cell viability as a function of maturation
(1, 7 and 30 days) for each material tested. Comparison of
relevant means was performed using the post hoc Bonfer-
roni test. Differences between groups was deemed signifi-
cant when P B 0.05.
3 Results
3.1 Material characterization
Characterization techniques utilized for this study includes
scanning electron microscopy (SEM/EDX), XRD and
HSM. SEM and the corresponding EDX are presented in
Fig. 1 for Ly-N, Ly-C and Ly-S. Figure 1a presents Ly-
N which confirms the composition contains Si4?
, Ca2?
,
Ti4?
and Na?
, which has a composition similar to batch
Table 2 Ionic composition of SBF [2]
Orders Reagents Amount
1 NaCl 7.996 g
2 NaHCO3 0.350 g
3 KCl 0.224 g
4 K2HPO4Á3H2O 0.228 g
5 MgCl2Á6H2O 0.305 g
6 1 M-HCl 40 ml
7 CaCl2 0.278 g
8 Na2SO4 0.071 g
9 NH2C(CH2OH)3 6.057 g
J Mater Sci: Mater Med
123
Author's personal copy
7. calculations. Minor differences include Si4?
slightly higher
at 60 % and Na?
at 11 %. Figure 1b presents Ly-C which
has a composition similar to the batch calculations, addi-
tionally, minor difference include Si4?
at 57 % and Ca2?
slightly lower than batch calculations at 19 %. Both Na?
and Sr2?
levels were determined to be 9 %, similar to batch
calculations. Figure 1c presents Ly-S which also has a
composition close to the original batch calculation. Very
slight compositional differences include Si4?
being 56 %
and Ca2?
being slightly lower than original calculations at
20 %. XRD patterns for each material are presented in
Fig. 2 with the complete list of crystal phases and crystal
size for Ly-N, Ly-C and Ly-S. Ly-N presented in Table 3.
The crystal phases for Ly-C contain sodium calcium sili-
cate phases (combeite) in addition to SiO2. Ly-S was found
to contain multiple crystal phases and each is listed in
Table 3, however as there is no Na?
in the starting com-
position, no sodium calcium silicate phases (combeite)
exist. Additionally, the crystal size for each phase was
calculated and for Ly-N the mean crystal size was 601 A˚ ,
for Ly-C the crystal size exceeded 1,000 A˚ and for Ly-S the
mean crystal size was 348 A˚ . HSM data is presented in
Fig. 3 and presents the sintering (Ts), softening (Tf) and
melting (Tm) temperature of Ly-N, Ly-S and Ly-C. From
Fig. 3 it is evident the thermal properties of each material
change as the Na?
/Sr2?
concentration differs. Regarding
Ly-N the Ts was found to be 653 °C, however as Sr2?
is
substituted the Ts increases to 713 °C (Ly-C) and 825 °C
(Ly-S). Regarding the Tf it was found to decrease from
866 °C (Ly-N) to 745 °C (Ly-C) and to increase to
1,243 °C (Ly-S) as the Sr2?
concentration increased. The
Tm for Ly-N and Ly-C were similar at 1,068 and 1,115 °C,
respectively, whereas Ly-S was found to be 1,244 °C.
3.2 Evaluation of surface dissolution and reactivity
SBF testing was conducted on glass and ceramic disc
samples with respect to (1) composition, (2) amorphous
and crystalline structure and (3) incubation time, over 1, 7
and 30 days. With respect to Figs. 4, 6 and 8, images are
presented at 1, 7 and 30 days for both the amorphous and
crystalline analogues, and the corresponding EDX of the
30 day samples. Figure 4 presents the Ly-N SBF results
where after 1 day incubation there was no CaP deposition
present. It is evident from Fig. 4 that after 7 days
Fig. 2 X-ray diffraction of a amorphous materials and b glass–
ceramic materials
Table 3 Crystal phases identified for Ly-N, Ly-C and Ly-S (see
Fig. 1)
Phase ID Reference
codes
Crystal size
(A˚ )
Ly-N
Combeite - Na2.2Ca1.9Si3O9 04-04–2757 612
Sodium: Na2Ca3Si6O16 04-012–8681 591
Ly-C
Combeite: Na4.8Ca3Si6O18 04-007–5453 [1,000
Silicon dioxide: SiO2 04-007–5453 [1,000
Ly-S
Strontium silicon: Sr2Si3 01-089–2593 348
Titanium oxide: Ti8O15 04-007–0444 138
Calcium silicon: CaSi2 04-007–0647 229
Strontium silicide: SrSi 01-076–7303 317
Strontium titanium silicate:
Sr2TiSi2O8
04-006–7366 261
Silicon oxide: SiO2 00-029–0085 [1,000
Perovskite: CaTiO3 04-015–4851 229
Fig. 3 Hot stage microscopy testing of Ly-N, Ly-C and Ly-S present-
ing sintering, softening and melting temperature for each material
J Mater Sci: Mater Med
123
Author's personal copy
8. incubation, the amorphous Ly-N surface was completely
covered in CaP. The crystalline counterpart did experience
CaP deposition, however it was minimal compared to the
amorphous Ly-N. However, after 30 days the surfaces of
the amorphous and crystalline analogues of Ly-N were
fully covered in CaP. The corresponding EDX detected the
presence of phosphate at *9 and *4 wt% for the amor-
phous and crystalline Ly-N, respectively. Figure 5 presents
the 30 days SEM images of the amorphous and crystalline
Ly-N at 1, 5 and 50k magnification. With respect to
Ly-C (Fig. 6) the surface of the amorphous Ly-C is similar
to Ly-N amorphous; however the crystalline counterpart
lacks the porous surface presented by Ly-N crystalline. CaP
deposition can be seen on the 7 days Ly-C amorphous and
the surface is fully covered after 30 days. Corresponding
EDX from 30 day samples presents high P levels in the Ly-
C amorphous, however a relatively low P signal was
present for Ly-C crystalline.
In order to investigate any changes in the surface of the
materials with respect to incubation time, XRD was con-
ducted on the samples that presented complete coverage by
CaP both after 30 days incubation in SBF. Figure 7 pre-
sents diffraction patterns of Ly-N (amorphous and crystal-
line) and Ly-C (amorphous) before and after 30 days
immersion in SBF. From Fig. 7a is evident that after
30 days crystal peaks are forming from the initially glassy
structure, which can be directly attributed to the deposition
of CaP as the starting material is amorphous. Crystal peaks
were identified as CaP (PDF 04-014-2292, Ca3(PO4)2)
which suggests that the crystal phase is an immature form
of hydroxyapatite. Figure 7b presents the crystalline ana-
logue of Ly-N which is initially predominantly crystalline.
However, after 30 days in SBF, the region corresponding
to 5–30° 2h exhibit a relatively minor amorphous region in
addition to the loss of a number of peaks in the 5–30° 2h,
and between 50 and 75° 2h. This is indicative of a poorly
Fig. 4 SEM images of amorphous and crystalline Ly-N after 1, 7 and 30 days in SBF and 30 day EDX of the amorphous and crystalline surfaces
J Mater Sci: Mater Med
123
Author's personal copy
9. crystalline CaP surface layer which is predominantly
amorphous with minor crystal formation. Attempts to
identify any newly formed crystal regions were inconclu-
sive. Figure 7c presents Ly-C amorphous which also pre-
sents a number of minor crystal peaks which are present at
55 and 60° 2h. However, this was less pronounced than
Ly-N amorphous which made phase identification difficult.
Regarding Ly-S, SEM imaging and the corresponding
30 days EDX are presented in Fig. 8. With respect to the
amorphous samples, CaP deposition can be observed at
each time period (1, 7 and 30 days), however to a much
lesser degree than the Na?
containing glasses. It is evident
from the EDX that after 30 days CaP is localized, and is
not as prevalent in density as Ly-N amorphous and
Fig. 5 Low and high magnification SEM images of Ly-N amorphous and crystalline samples after 30 days in SBF
J Mater Sci: Mater Med
123
Author's personal copy
10. crystalline and Ly-C amorphous. With respect to the
crystalline analogue of Ly-S, there was relatively minor
detection of P at less than 0.5 wt% for each time period.
When comparing the CaP structures formed with respect to
each material (Fig. 9), it is evident that different mor-
phologies exist for both Na?
and Sr2?
containing materials.
Figure 9a presents Ly-N at 7 days displaying full surface
coverage with highly irregular, plate-like crystal formation.
Figure 9a presents the intermediate glass (Ly-C) at 7 days
which predominantly displays needle-like crystals that
seem to be growing epitaxially, in addition, it is evident
that precipitation is beginning on the particles on the left of
the same image. This may be the beginning the formation
of plate-like crystal similar to the Ly-N as after 30 days the
final morphology is similar to that of Ly-N at 30 days. With
respect to Ly-S, CaP deposition presented spherical glob-
ular like projections that were far less abundant that the
plate-like projections presented on Ly-N and Ly-C.
3.3 Evaluation of cytocompatibility
Regarding cell culture analysis, Fig. 10a presents the
cytocompatibility of the amorphous samples while
Fig. 10b presents the crystalline analogues. It is evident
from Fig. 10a that Ly-N presented a steady increase in cell
viability. The increase was not deemed significant at 1 day
(94 %, P = 1.000) or 7 day (111 %, P = 0.900), however
at 30 days a significant increase was observed (134 %,
P = 0.0001). Ly-C amorphous remained relatively con-
stant over each time period with no significant change
(P = 0.037–0.197) when compared to the growing cell
population. The Sr2?
containing series, Ly-S, produced
significantly lower cell viability at 1 day (70 %,
P = 0.007) and 7 day (59 %, P = 0.0001) however, no
significant change in viability (68 %) was observed at
30 days. With respect to the crystalline analogues there
was no significant increase in cell viability for any of the
Fig. 6 SEM images of amorphous and crystalline Ly-C after 1, 7 and 30 days in SBF and 30 day EDX of the amorphous and crystalline surfaces
J Mater Sci: Mater Med
123
Author's personal copy
11. Fig. 7 XRD of samples with surface changes in SBF before and after 30 days incubation
Fig. 8 SEM images of amorphous and crystalline Ly-S after 1, 7 and 30 days in SBF and 30 day EDX of the amorphous and crystalline surfaces
J Mater Sci: Mater Med
123
Author's personal copy
12. materials. There was a significant decrease in viability for
Ly-C at 7 days (59 %, P = 0.000), and 30 days (71 %,
P = 0.001). Ly-S also presented an overall reduction in cell
viability; however this change did not reach significance.
4 Discussion
4.1 Material characterization
SEM coupled with EDX was used to image the glass
particles and estimate the glass composition. The mean
particle size was previously determined for each glass,
which were similar at 4.6 lm for Ly-N, 3.9 lm for
Ly-C and 4.6 lm for Ly-S [27]. Particle size distribution
can significantly influence biocompatibility through chan-
ges in exposed surface area and the subsequent particle
dissolution rate. It can also be observed that the Na?
containing glasses (Ly-N, Ly-C) were found to have smaller
particles agglomerated to larger particles, in particular with
Ly-N. This agglomeration of the Na?
containing glasses
may be due to electrostatic charge build up during pro-
cessing. With respect to glass composition, the batch
calculation closely resembled the data acquired by EDX.
XRD was conducted on each material to confirm the
amorphous/crystalline state and to determine the phases
present in the crystalline materials. Sodium calcium silicate
phases were found to exist in Ly-N and Ly-C, whereas
numerous phases were detected in Ly-S (Table 3). Previous
studies suggest that crystallization converts glass from
being a bioactive to an inert material and, in the case of
glass–ceramic scaffolds, the mechanical integrity is also
compromised. However additional studies by Chen et al.
[28] suggest that crystalline 45S5 BioglassÒ
forms Na2-
Ca2Si3O9 phases that can significantly improve the
mechanical properties of the material, that crystallization
does not inhibit bioactivity with respect to bone bonding
ability and when immersed in body fluids the crystalline
Na2Ca2Si3O9 decomposes and transits to amorphous CaP.
Studies by Clupper and Hench [20] determined that the
predominant crystal phase associated with BioglassÒ
,
Na2Ca2Si3O9 slightly decreases the formation kinetics of
an A layer on BioglassÒ
surface but did not totally suppress
its formation. Further studies by Filho et al. [21] found that
there is no compromise in bioactivity for the 45S5 glass–
ceramic system even when 100 % crystalline. Their study
Fig. 9 SEM images of CaP deposition on a Ly-N, b Ly-C and c Ly-S
Fig. 10 Cell viability of
amorphous and crystalline
samples
J Mater Sci: Mater Med
123
Author's personal copy
13. in particular focused on determining the surface precipi-
tation reactions in SBF and determined that by inducing
crystallinity (ranging from 8 to 100 %), the materials
maintained their bioactivity in SBF [21]. HSM determined
that differences in the thermal properties of the materials
existed where the sintering temperature increases with an
increase in Sr2?
concentration, i.e. Ly-N Ly-C
Ly-S. This may be due to the divalent nature of Sr2?
,
charge neutralizing intermittent non bridging oxygen sites
in the glass, where two Na?
atoms are required to fulfill the
same vacancy. This would likely result in higher temper-
atures to decompose the Ly-S glass particles to the extent
required for sintering, as compared to Ly-N.
4.2 Evaluation of surface characteristics and reactivity
With respect to the amorphous surface for each material,
differences in surface morphology exist where the particles
are fused together resulting in a ‘cobblestone’ like surface.
The crystalline analogues for each material formed a por-
ous/dense or irregular surface morphology relatively spe-
cific to the materials composition. CaP deposition on the
Ly-N supports earlier findings by Clupper and Hench,
where the formation of an A layer was decreased but not
totally suppressed. The higher resolution images (Fig. 5)
confirm the complete coverage of both Ly-N amorphous
and crystalline surfaces with CaP. Higher resolution SEM
images (50k) present a floral-plate like CaP present for
both materials, which is similar in structure to A deposition
on A–W glass–ceramic [2]. This suggests that the CaP
deposited is similar irrespective of amorphous/crystalline
structure, with the only difference being the time required
for this surface layer to deposit. Ly-C amorphous presented
CaP deposition the covered the surface after 30 days. The
lack of surface precipitation on Ly-C crystalline may be
due to a number of factors. The lower Na?
concentration in
the starting glass results in reduced solubility, which
reduces the rate of ion release from the material. Previous
ion release studies on these materials by the authors
resulted in the Na?
containing materials being more solu-
ble than the Sr2?
(i.e. Ly-N [ Ly-C [ Ly-S) [27]. In
addition, the amorphous materials were found to be far
more soluble than the crystalline analogues [27]. XRD
conducted on samples with full CaP surface coverage
determined that the evolution of crystal structures was
identified predominantly in samples containing Na?
.
Although the amorphous samples Ly-N and Ly-C induced
complete surface coverage after 30 days, the crystalline
analogue of Ly-N produced the same surface morphology
which took longer to produce as there was no significant
coverage after 7 days, suggesting that a solubility limit
exists. The precipitation of the CaP crystals support this
where the Na?
containing materials produced plate-like
crystals (Fig. 9) after 30 days, whereas Ly-S produced
globular-like crystals which were only sporadically dis-
tributed on the surface after 30 days. Also, it seems the
reduced solubility in addition to the inclusion of Sr2?
,
results in an overall reduction in CaP deposition.
4.3 Evaluation of cytocompatibility
A general trend that can be observed with the cell viability
data is that as Sr2?
is incorporated into the glass, there is a
general reduction in cell viability. Ly-N produced the
highest cell viability after 30 days at 134 %, which is
likely attributed to the solubility [27] and the release of
Na?
. Na?
is well known to be critical to cellular metabo-
lism including providing the required ion concentration
gradients between the interstitial fluids and the cytoplasm.
As such, cells have the ability to tightly control the influx/
efflux of ions like Na?
, K?
and Ca2?
. The solubility of the
Ly-C and Ly-S is lower [27] than Ly-N which may be a
contributing factor to the reduction/insignificant change in
cell viability. In particular with Ly-S, it is also possible that
Sr2?
hinders the metabolic process in this particular cell
line, as positive reports have been established regarding
Sr2?
use in osteoblasts [24]. With respect to the crystalline
analogues, which were much less soluble than the amor-
phous counterparts [27], there were relatively insignificant
changes in cell viability. This may be due to a combination
of the reduced ion release rate, in addition to these cells not
requiring Sr2?
for metabolism and ion homeostasis.
5 Conclusion
The work determined that the Na?
containing glasses and
ceramics consistently produced CaP surface layers more
readily than the Sr2?
containing materials. However, dif-
ferent morphologies of CaP was observed with differences
in glass composition. Additionally, the CaP surface layer
was observed to crystallize after 30 days with the Na?
containing materials. Testing of the liquid extracts in cell
culture was observed to significantly increase cell viability
in the Na?
containing glasses, while no significant toxicity
was experienced with the Sr2?
and crystalline analogues of
each material. This study concludes that the inclusion of
Na?
significantly enhances the surface reactivity of
bioceramics, and that the addition of ions with different
electronic states can significantly influence the ion release
rate, atomic arrangement and morphology of the precipi-
tated surface A layer, and the associated cellular response.
J Mater Sci: Mater Med
123
Author's personal copy
14. References
1. Jones JR. Review of bioactive glass: from Hench to hybrids. Acta
Biomater. 2013;9:4457–86.
2. Kokubo T, Takadama H. How useful is SBF in predicting in vivo
bone bioactivity. Biomaterials. 2006;27:2907–15.
3. Kokubo T, Kim H-M, Kawashita M. Novel bioactive materials
with different mechanical properties. Biomaterials. 2003;24:
2161–75.
4. Hench LL. The story of Bioglass. J Mater Sci Mater Med. 2006;
17:967–78.
5. Hench LL. Genetic design of bioactive glass. J Eur Ceram Soc.
2009;29(7):1257–65.
6. Haimi S, Gorianc G, Moimas L, Lindroos B, Huhtala H, Raty S,
Kuokkanen H, Sandor GK, Schmid C, Miettinen S, Suuronen R.
Characterization of zinc-releasing three-dimensional bioactive
glass scaffolds and their effect on human adipose stem cell pro-
liferation and osteogenic differentiation. Acta Biomater.
2009;5(8):3122–31.
7. Vargas GE, Mesones RV, Bretcanu O, Lo´pez JMP, Boccaccini
AR, Gorustovich A. Biocompatibility and bone mineralization
potential of 45S5 BioglassÒ
-derived glass–ceramic scaffolds in
chick embryos. Acta Biomater. 2009;5(1):374–80.
8. Chen QZ, Rezwan K, Franc¸on V, Armitage D, Nazhat SN, Jones
FH, Boccaccini AR. Surface functionalization of BioglassÒ
-
derived porous scaffolds. Acta Biomater. 2007;3(4):551–62.
9. Anderson JH, Goldberg JA, Bessent RG, Kerr DJ, McKillop JH,
Stewart I, Cooke TG, McArdle CS. Glass yttrium-90 micro-
spheres for patients with colorectal liver metastases. Radiother
Oncol. 1992;25(2):137–9.
10. Bortot MB, Prastalo S, Prado M. Production and characterization
of glass microspheres for hepatic cancer treatment. Proc Mater
Sci. 2009;1:351–8.
11. da Costa Guimaraes C, Moralles McRoberto Martinelli J. Monte
Carlo simulation of liver cancer treatment with 166Ho-loaded
glass microspheres. Radiat Phys Chem. 2014;95:185–7.
12. Ladron de Guevara-Fernandez S, Ragel CV, Vallet-Regi M.
Bioactive glass-polymer materials for controlled release of ibu-
profen. Biomaterials. 2003;24(22):4037–43.
13. Zhao L, Yan X, Zhou X, Zhou L, Wang H, Tang J, Yu C.
Mesoporous bioactive glasses for controlled drug release.
Microporous Mesoporous Mater. 2008;109:210–5.
14. Ravarian R, Moztarzadeh F, Hashjin MS, Rabiee SM, Khoshakh-
lagh P, Tahriri M. Synthesis, characterization and bioactivity
investigation of bioglass/hydroxyapatite composite. Ceram Int.
2010;36(1):291–7.
15. Goller G. The effect of bond coat on mechanical properties of
plasma sprayed bioglass–titanium coatings. Ceram Int.
2004;30(3):351–5.
16. Goller G, Demirkiran H, Oktar FN, Demirkesen E. Processing
and characterization of bioglass reinforced hydroxyapatite com-
posites. Ceram Int. 2003;29(6):721–4.
17. Habibe AF, Maeda LD, Souza RC, Barboza MJR, Daguano
JKMF, Rogero SO, Santos C. Effect of bioglass additions on the
sintering of Y-TZP bioceramics. Mater Sci Eng C. 2009;29(6):
1959–64.
18. Kashyap S, Griep K, Nychka JA. Crystallization kinetics, min-
eralization and crack propagation in partially crystallized bioac-
tive glass 45S5. Mater Sci Eng C. 2011;31(4):762–9.
19. Chen QZ, Thompson ID, Boccaccini AR. 45S5 BioglassÒ
-
derived glass–ceramic scaffolds for bone tissue engineering.
Biomaterials. 2006;27(11):2414–25.
20. Clupper DC, Hench LL. Crystallization kinetics of tape cast
bioactive glass 45S5. J Non-Cryst Solids. 2003;318:43–8.
21. Filho OP, LaTorre GP, Hench LL. Effect of crystallization on
apatite-layer formation of bioactive glass 45S5. J Biomed Mater
Res. 1996;30:509–14.
22. Chen X, Meng Y, Li Y, Zhao N. Investigation on bio-minerali-
zation of melt and solgel derived bioactive glasses. Appl Surf Sci.
2008;255(2):562–4.
23. Serra J, Gonzalez P, Liste S, Chiussi S, Leon B, Perez-Amor M,
Ylanen HO, Hupa M. Influence of the non-bridging oxygen
groups on the bioactivity of silicate glasses. J Mater Sci Mater
Med. 2002;13:1221–5.
24. Marie PJ. Strontium ranelate; a novel mode of action optimizing
bone formation and resorption. Osteoporos Int. 2005;16:S7–10.
25. Marie PJ. Strontium ranelate: new insights into its dual mode of
action. Bone. 2007;40:S5–8.
26. Takadama H, Kim HM, Kokubo T, Nakamura T. XPS study of
the process of apatite formation on bioactive Ti–6Al–4V alloy in
simulated body fluid. Sci Technol Adv Mater. 2001;2:389–96.
27. Li Y, Coughlan A, Laffir FR, Pradhan D, Mellott NP, Wren AW.
Investigating the mechanical durability of bioactive glasses as a
function of structure, solubility and incubation time. J Non-Cryst
Solids. 2013;380:25–34.
28. Chen QZ, Li Y, Jin LY, Quinn JMW, Komesaroff PA. A new
sol–gel process for producing Na2O-containing bioactive glass
ceramics. Acta Biomater. 2010;6:4143–53.
J Mater Sci: Mater Med
123
Author's personal copy