3. A micromanipulator is a device which is used to
physically interact with a sample under a
microscope
It may typically consist of an input joystick ,
a mechanism for reducing the range of
movement and an output section with the
means of holding a microtool to hold, inject,
cut or otherwise manipulate the object as
required
Micromanipulators are usually used in
conjuction with microscope.
It is the method used in the isolation of pure
culture technique
4.
5. Micromanipulator have been
built, which permit one to pick
out a single cell from a mixed
culture.
This instrument is used in
conjuction with a microscope to
pick a single cell (particularly
bacterial cell)from a hanging
drop preparation.
6. The micromanipulator have a micrometer
adjustments by means of which its
micropipette can be move right and left,
forward and backward, and up and down.
A series of hanging drop of a diluted
culture are placed on a special sterile
coverslip by a micropipette.
Now a hanging drop is searched, which
contains only a single microorganism cell
7.
8. This cell is drawn into the micropipette
by gentle suction and then transferred to
a large drop of sterile medium on another
sterile coverslip.
When the number of cell increases in that
drop as a result of multiplication, the
drop is transferred to a culture tube
having suitable medium.
This yield a pure culture of the required
microorganism.
9.
10. The advantage of this method are
that can be reasonably sure that
the cultures come from a single
cell and one can obtain strains
with in the species.
