Introduction of Mycobacterium tuberculosis, quinoline and their biological importance. Design, diversity oriented synthesis, primary antimycobacterial activity evaluation against M.smegmatis strain and library generation of bedaquiline related quinolinyl heterocycles. Structure optimization of obtained active hits by using molecular hybridization, ring functionalization and antimycobacterial activity evaluation against M.smegmatis strain. Low cytotoxicity against A549 cells highlighted the efficiency of the hits. Also antimicrobial and anti-inflammatory activities were also carried out. BDMS catalyzed one pot multicomponent synthesis of imidazo[1,2-a]pyridine and Friedlander synthesis of quinolone under solvent free conditions.

1. QUINOLINYL HETEROCYCLES AS ANTIMYCOBACTERIALAGENTS:
DESIGN DIVERSITY-ORIENTED SYNTHESIS, STRUCTURE ACTIVITY
RELATIONSHIPAND ACTIVE ANALOGUES OPTIMIZATION
Thesis Submitted
To
Department of Chemistry
Kakatiya University, Warangal
For the degree of
Doctor of Philosophy in Chemistry
By
Rachakonda Venkatesham, UGC-SRF,
Under the Supervision of
Dr. A. Manjula, Principal Scientist
Crop Protection Chemicals (Organic-II) Division
CSIR-I.I.C.T
Crop Protection Chemicals Division (Organic-II)
CSIR-Indian Institute of Chemical Technology
Hyderabad-500607, India
July-2014

2. Thesis
 Conclusions
 Acknowledgements

3. Chapter-I
Introduction of Mycobacterium tuberculosis, Quinoline and their
Biological importance

4. Tuberculosis (TB)
 Tuberculosis (TB) is the leading infectious disease in the world.
 It is caused by the bacillus Mycobacterium tuberculosis (MTB) and was discovered
by Robert Koch in 1882.
 Latent TB
 Drug sensitive Tuberculosis-curable with first line drugs (INH, PZA, EMB, RIF)
 MDR-TB-resistant to RIF and INH, treatment FQs & inject able drugs AMK,
KAN, CAP.
 XDR-TB resistant to at least one FQs and inject able drugs
 TDR-TB incurable form of TB
 HIV- Co-Infection
DOTS strategy
BCG-Vaccination

5. Figure 2: M. tuberculosis aerosol transmission and progression to infectious TB or
non-infectious (latent) disease

6. Figure 1: Available TB drugs

7. Figure 5: Pictorial representation of recently approved and under clinical trial TB active
compounds and their targets

8.  TMC-207 (Badaquiline) is a diaryl quinolinyl US-FDA approved TB drug.
 Trade name is Sirturo.
 Effectively inhibits the Mycobacterium smegmatis strain.
TMC-207 (Bedaquiline)
TB Bacteria
Figure 2: TMC-207 activity on TB bacteria

9. Figure 4: Classification of quinoline synthesis, based on starting material

10. Bedaquiline

11. Figure 2. Extrapolation of isoniazid and bedaquiline
Isoniazid

13. Figure 5: Synthetic strategy of TMC-207 based analougues

15. Chapter-I
Design, diversity-oriented synthesis and structure activity
relationship studies of quinolinyl heterocycles as
antimycobacterial, antimicrobial and anti-inflammatory agents

18. Scheme 3: Oxidative condensation for C4 unsubtituted quinoline synthesis

21. Scheme 8: Synthesis of 2-(2-methylquinolin-3-yl)-5-phenethyl-1,3,4-oxadiazole

22. Scheme 9: Synthesis of 2-bromo-1-(2-methylquinoln-3-yl)ethanone

23. Scheme 10

24. Scheme 11: Synthesis of 3-(dimethylamino)-1-(2-methylquinoline-3-yl)prop-2-one

25. Scheme 12

27. Scheme 15: Synthesis of 1-(2-methylquinolin-3-yl)-3-(2,4,6-trimethoxyphenyl)prop-2-en-1-one

28. Scheme 16: Synthesis of 1-(3-(2-methylquinolin-3-yl)-5-(2,4,6-trimethoxyphenyl)-
4,5-dihydro-1H-pyrazol-1-yl)ethanone

29. Compound Antimycobacterial activity
(against M. smegmatis strain)
Cytotoxicity activity
(against A549 cells)
a &b MIC(μg/mL) cStd. Dev. dIC50 (μM) Std. Dev
86 >100 - 96.373 20.335
89a 34.97 4.2 144.839 28.677
91 >100 - >200 -
92 >100 - 117.724 32.516
94 >100 - 94.198 18.000
95 14.66 1.25 137.510 4.534
102a 57.87 13.18 170.745 48.263
Rifampicin (Control) 2.08 0.04 ----- ------
Isoniazid (Control) 12.07 0.98 ---- ----
aMycobacterium smegmatis ATCC 14468 (MC2).
b Concentration of compounds inhibiting growth by 50%
c GI50 values are indicated as mean ± SD (standard deviation) of three independent experiments.
dIC50 (μM) against A549 cells
Table 1: Primary antimycobacterial and cytotoxicity screening of diverse quinolinyl
heterocycles

30. Figure 14: Graphical representation of activity results

31. Scheme 18: Synthesis of 2,5-disubstituted quinolinyl 1,3,4-oxadiazole (26) derivatives

32. Scheme 19: Synthesis of N-acyl quinolinyl pyrazoline (16) derivatives

33. Scheme 20: Synthesis of N-phenyl quinolinyl pyrazoline derivatives

34. Scheme 21: BDMS catalyzed synthesis of 3-(tetrahydro-2H-pyran-2-yl)pentane-2,4-dione

35. Scheme 22

36. Scheme 24: Synthesis of 1-(3-(2-methylquinolin-3-yl)-1H-pyrazol-1-yl)ethanone

37. 52 compounds synthesized
40 compounds tested

38. Mycobacterium
Tuberculosis
Antimicrobial
Biological
Activities
Anti-
Inflammatory

39. Antimycobacterial activity Cytotoxicity activity Antimycobacterial activity Cytotoxicity activity
A Ba&b CC Dd Cc
A Ba&b CC Dd Cc
89b 35.93 9.62 164.512 13.317 102d >70 - 110.856 21.568
89c 35.04 14.92 174.567 22.078 102e 52.09 13.77 >200 -
89d >70 - 145.988 24.743 102f >70 - >200 -
89e >70 - 72.159 20.916 102h >70 - >200 -
89f 41.05 13.0 103.472 16.873 102i >70 - 93.330 14.414
89g 29.18 10.71 134.278 51.084 102j 50.70 1.59 >200 -
89h 61.88 15.23 113.023 2.596 102k 64.90 12.13 72.726 4.731
89i 43.29 10.0 …….. 0.657 102l >70 - >200 -
89j 22.71 4.0 138.767 17.112 102m >70 - 114.596 6.073
89k 47.53 9.03 93.314 11.475 102n >70 - 166.047 83.831
89l >70 - 91.132 4.406 106a >70 - 92.976 11.253
89m 16.83 5.93 111.013 4.083 106b >70 - >200 -
89n 39.73 10.61 99.364 15.649 106c 43.52 4.36 142.803 61.457
89o >70 - >200 - 109a 24.11 6.71 97.873 21.434
102b >70 - 110.429 21.661 109b >70 - 142.528 38.767
102c >70 - 115.977 15.083 112 17.34 4.21 87.025 8.86
X 2.15 0.57 ----- ----- X 2.15 0.57 ----- -----
Y 11.78 0.86 ----- ----- Y 11.78 0.86 ----- -----
A: Compunds, B: MIC (µg/mL), C: Std dev, D: IC50 (µm/mL), a Mycobacterium smegmatis ATCC 14468 (MC2).
b Concentration of compounds inhibiting growth by 90 %. c MIC values are indicated as mean ± SD (standard deviation), dIC50
(μg/mL) against A549 cells, X: Rifampicin, Y: Isoniazid
Table 2: Antimycobacterial activity of quinolinyl oxadiazoles pyrazolines and
pyrazoles

40. Figure 16: Graphical representation of antimycobacterial active compounds
Design and diversity oriented synthesis of novel quinoline containing heterocycles has been
achieved employing simple reaction protocols and common intermediates. Simultaneous screening for
antimycobacterial activity led to the identification of quinolinyl oxadiazole and pyrazole frameworks as
active molecules. The synthetic protocols have been successfully extended for generation of library of
uinolinyl oxadiazoles, pyrazolines and pyrazoles. In all, 40 compounds were tested for antimycobacterial
activity and lead identification gave of 20 active molecules and 3 (8, 12j and 12m) promising
compounds. Further, lowcytotoxic effects of these compounds against A549 cell line underline their
potential as antitubercular agents.
• Finding of this results have been published in Eur. J. Med. Chem. 70 (2013) 536-547
Venkatesham Rachakonda, Manjula Alla, Sudha Sravanti Kotapalli, Ramesh Ummanni

41. Gram negative bacterial strains Gram positive bacterial strains
Comp a b c d e f g h i j
89n -- -- -- -- 0.14 0.14 0.13 -- -- --
89r -- 0.14 -- 0.13 -- -- -- -- -- --
89s 0.14 -- -- 0.13 0.11 -- 0.11 0.14 -- --
89w 0.14 -- -- -- -- -- -- -- -- --
89y -- -- -- -- 0.15 -- 0.15 -- -- --
Std -- 0.18 0.22 -- -- 0.18 0.14 0.16 0.22 0.18
Std (Standard): Streptomycin (Concentration 10μg/mL)
a= Eschericha coli, b= Proteus vulgaris, c= Proteus mirabilis, d= Klebsiela
pneumoniae, e= Enterobacter aerogens, f= Bacillus subtilis, g= Bacillum
megaterium, h= Bacillus pumilis, i= Staphylococcus aureus, j= Streptococcus
pyogens and-- = Not active
Antibacterial activity (MIC 10µg/mL) of 2-(2-methylquinolin-3-yl)-5-substituted-1, 3, 4-oxadiazoles
Five of the seventeen
compounds tested showed
impressive MIC values
(10μg/mL). Compounds 89w,
89s showed effective inhibition
on E. coli at 10µg/mL
concentration. Compounds 82r,
82s and 89n, 89s, 89y showed
effective inhibition on Klebsiella
pneumoniae and Enterobacter
aerogenes, at 10µg/mL
concentration, respectively. At
this concentration (10μg/mL),
standard has not shown any
inhibition of these species.
Among all the tested
compounds, 89w, 89n, 89r, 89s,
89y effectively inhibited the
growth of resistant gram
negative bacteria compared to
gram positive bacteria at
10µg/mL concentration

42. Antifungal activity of 2-(2-methylquinolin-3-yl)-5-substituted-1, 3, 4-oxadiazoles
Zone of inhibition ( mm)
A B a b c d A B a b c d
89a
600 4.50 5.03 7.59 5.56
89o
600 NA 4.35 3.54 5.09
900 8.45 10.73 14.45 10.67 900 NA 8.45 6.23 10.45
89b
600 4.26 5.35 3.32 5.92
89q
600 5.56 4.32 7.65 6.32
900 7.72 10.85 6.54 11.23 900 11.05 8.25 14.34 12.35
89c
600 5.05 6.32 6.59 6.90
89r
600 6.25 4.05 3.50 3.45
900 10.23 13.16 14.12 13.06 900 12.24 8.25 6.45 6.56
89d
600 5.32 5.56 4.56 3.23
89s
600 4.56 6.32 8.56 4.45
900 10.82 11.32 8.89 6.67 900 8.23 12.34 16.24 9.05
89e
600 3.34 5.54 4.34 2.12
89v
600 4.12 5.03 5.23 4.05
900 6.21 10.21 8.21 4.34 900 8.45 10.34 10.56 8.10
89f
600 3.42 4.26 3.25 3.45
89w
600 5.65 4.45 3.32 5.56
900 6.75 8.59 6.50 7.14 900 10.25 9.08 6.67 10.54
89g
600 3.12 4.98 3.92 5.39
89I
600 2.13 5.32 6.87 5.23
900 6.23 9.43 7.42 11.12 900 4.32 10.64 12.21 10.54
89n
600 6.50 6.25 NA 5.35
……..
……… ……… ……… ……… ……….
900 12.23 12.87 NA 11.05 ……… ……… ……… ……… ………
X
600 5.35 10.80 7.56 9.34
X
600 5.35 10.80 7.56 9.34
900 12.01 20.03 14.76 18.26 900 12.01 20.03 14.76 18.26
a=Candida albicans, b=Fusarium oxysporium, c=Dreschleria halides, d=Colletotrichum falcatum; NA=No Activity; B: Itraconazole
(Standard)
An extra heterocycle substitution on oxadiazole enhances the antifungal activity. 3-[5-(4-
Methoxyphenyl)-[1,3,4]oxadiazol-2-yl]2-methylquinoline (89r) is the other compound which
inhibited fungal growth to significant extent.

43. Table 7: Anti-inflammatory activity of 2-(2-methylquinolin-3-yl)-5-substituted-1, 3, 4-oxadiazoles by
carrageenan-induced rat paw edema assay (acute inflammatory model)
Comp Dose
(mg/kg)
Percentage of Edema Inhibition
(%)
30 mins 1h 2h 3h
89b 100 28 35.2 51.6 51.7
89c 100 24 35.2 60 49.4
89d 100 16 26.4 28 24.1
89f 100 32 38.2 57.3 48.2
89g 100 16 35.2 45.3 24.1
89n 100 40 41.1 60 67.8
89o 100 36 44.1 54.6 58.6
89q 100 20 35.2 41.3 42.5
89r 100 12 17.6 40 42.5
89y 100 20 38.2 56 50.5
89I 100 36 50 50.6 57.4
Std
(Diclofenac)
10 40 41.1 52 68.9
The anti-inflammatory activity profile indicates that compounds with heterocyclic substitution at 5th
position of oxadiazole is again the best candidate among the tested molecules. Thiophene substitution
shows better anti-inflammatory activity than standard at 1 h, 2 hrs, 3 hrs time but activity falls at 4
hrs, while in case of pyrazole substitution activity falls from 3 hrs. 2-(2-Methylquinolin-3-yl)-5-(1-
phenylethyl)-1,3,4-oxadiazole (89b) is the only alkyl substitution that exhibits good activity.

44. Chapter-III
Quinolinyl Heterocycles as Antimycobacterial Agents: Towards
Structure Optimization of the Acive Analogues 2-Methyl-3-(1H-
pyrazol-5-yl)quinoline and 3-(5-(2-methylquinolin-3-yl)-1,3,4-
oxadiazol-2-yl)4H-chromen-4-one

45. In all, 40 compounds were tested for antimycobacteium activity and lead identification
gave of 20 active molecules and 2 (1, 2) promising compounds
Compound MIC(μg/mL)
2 14.66
Rifampicin (Control) 2.08
Isoniazid (Control) 12.07
Compound MIC(μg/mL)
1 16.83
Rifampicin(Control) 2.15
Isoniazid (Control) 11.78

47. Scheme 1: Synthesis of 2-methylquinoline-3-carbohydrazone (10) derivatives

48. Figure 6: Various positions for the synthesis of substituted 2-methyl-3-(1H-pyrazol-5-yl)quinoline

49. Scheme 4: Synthesis of 2-methyl-3-(1-(N-aryl/alkyl)-1H-pyrazol-3-yl)quinoline derivatives

51. Figure 8: Retrosynthetic analysis for ring functionalization of pyrazole

52. Scheme 5: Reaction of active methylene group with triethyl orthoformate and DMF-DMA.
Reactive intermediate

53. Scheme 2: Synthesis of ethyl 3-(2-methylquinolin-3-yl)-3-oxopropanoate

54. Scheme 7: Synthesis of ethyl 3-(2-methylquinolin-3-yl)-1H-pyrazole-4-carboxylate

55. Scheme 8: Synthesis of 3-(2-methylquinolin-3-yl)-1H-pyrazol-5-ol (23)

56. Scheme 10: Synthesis of 2-methyl-3-(1-(prop-2-ynyl)-1H-pyrazol-3-yl)quinoline or
ethyl 3-(2-methylquinolin-3-yl)-1-(prop-2-ynyl)-1H-pyrazole-4-carboxylate

57. Figure 15: Molecular hybridization approach for structure optimization

58. Scheme 3. Synthesis of quinolinepyrazolyl 1,2,3-triazole hybridized moecules (21 molecules)
starting from common intermediates 13 and 14

63. Antimycobacteria
l activity
Cytotoxicity Antimycobacterial
activity
Cytotoxicity
A Ba&b Cc Dd Cc A Ba&b Cc Dd Cc
12a 28.8 0.032 >100 52b 33.76 0.01 36.6 17.52
12b >100 22.16 13.2 52c >100 12.35 0.26
12c 19.57 0.004 >100 52d >100 21.02 1.23
12d 17.26 0.003 >100 52e >100 >100
12e 21.62 0.050 >100 52f NA 23.65 8.13
12f >100 >100 52g >100 >100
12g 23.5 0.038 8.29 6.63 52h >100 15.49 8.71
12h >100 10.86 2.09 52i NA >100
12i 14.92 0.012 15.82 8.29 53a >100 20.44 9.75
12j >100 17.08 11.84 53b 33.27 0.012 16.04 0.75
12k >100 23.85 2.3 53c 25.17 0.013 >100
36 NA 10.52 6.49 53d 26.34 0.004 22.88 15.36
20a >100 24.29 22.97 53e 29.87 0.006 16.442 4.69
20b 20.64 0.06 11.14 5.84 53f >100 22.25 11.79
20c >100 15.19 2.93 53g >100 >100
20d NA >100 53h >100 >100
22 NA >100 54a NA 20.114 6.92
37 28.26 13.19 3.96 54b 40.5 >100
23 >100 15.26 5.92 54c >100 >100
52a NA >100 55 >100 16.85 9.22
X 12.07 ------- X 12.07 -------
Y 2.15 ------- Y 2.15 -------
Z ------- ------- 0.16 Z ------- -------
A: Compound, B: MIC (µg/mL), C: STD Dev; aMycobacterium smegmatis ATCC 14468 (MC2155).
b Minimum concentration of compounds inhibiting visible bacterial growth. c MIC values are indicated as mean ±SD (standard deviation) of
three independent. d IC50 (µg/mL) against A549 cells.
X: Isoniazid, Y: Rifampicin, Z: Doxorubicin
Antimycobacterial evaluation of optimized quinolinyl heterocycles

64. • The MIC values (µg/ml) of compounds 14a (28.8±0.032), 14c (19.57±0.004),
14d (17.26±0.003), 14e (21.62±0.05), 37b (33.76 ± 0.01), 38c (25.17±0.013)
and 39b (40.5) compared to their cytotoxicity in IC50 (>100) highlight that these
are promising scaffolds to design and develop
quinolinyl based antimycobacterial agents.
• 14i showed cytotoxicity with IC50 value 32.70±8.29 µM while its MIC was found
to be 14.92±0.012 (µg/ml).
• Thus, this compound may not be a good antitubercular agent.
• Interestingly quinolinyl hydrazones were shown good antimycobacterial activity
than the corresponding pyrazolyl1,2,3-triazoles.
• It was initiated to gone for antibacterial activity of quinolinyl hydrazone

65. In vitro antibacterial activity of quinolinyl hydrazones
MIC (10μg/mL)
Gram Negative bacterial strains Gram Positive bacterial strains
Comp a b c d e f g h i j
12a 0.14 -- -- 0.11 -- -- 0.11 -- -- --
12b -- -- -- -- 0.15 0.15 -- 0.12(
25)
-- --
12c 0.11 -- -- -- 0.14 0.14 0.12 - -- --
12d 0.14 -- -- 0.13 0.11 -- 0.11 014 -- --
12e -- -- -- -- 0.14 -- 0.13 -- -- --
12f -- -- -- -- 0.15 -- 0.15 -- -- --
Std --- 0.18 0.22 --- --- 0.18 0.14 0.16 0.22 0.18
Std (Standard): Streptomycin a= Eschericha coli, b= Proteus vulgaris, c= Proteus mirabilis,
d= Klebsiela pneumoniae, e= Enterrobacter aerogens, f= Bacillus subtilis, g= Bacillum
megaterium, h= Bacillus pumilis, i= Staphylo coccusaureus, j= Streptococcus pyogens and -
-- Not Active.
Six of the ten
compounds tested showed
impressive MIC values
compared to streptomycin
which was used as standard
(10μg/mL) on different strains.
Among all the tested
compounds, 12(a-f) effectively
inhibited the growth of drug
resistant gram negative bacteria
compared to gram positive
bacteria at 10µg/mL
concentration. Graphical
representation of antibacterial
activity of quinolinyl
hydrazones in MIC is shown

66. • Selective structure optimization of the hits was attempted on 3-(5-(2-methylquinolin-
3-yl)-1,3,4- oxadiazol-2-yl)-4H-chromen-4-one and 2-methyl-3-(1H-pyrazol-5-
yl)quinoline molecules by both forward and reverse synthetic approaches.
• Molecular hybridization of 2-methyl-3-(1H-pyrazol-5-yl)quinoline employing the
copper catalyzed Huisgen’s 1,3-dipolar cycloaddition (Click reaction) led to
introduction of 1,2,3-triazole ring.
• 40 compounds synthesized were evaluated for their antimycobacterial activity and
cytotoxicity against A549 cell line.
• All Among the 14 antitubercular active compounds, 3 have shown promising activity
coupled with low cytotoxicity profile.
• Quinolinyl hydrazones were also evaluated for anti bacterial activity which shown
good results
• Finding of this results have been Communicated to
Eur. J. Med. Chem. Venkatesham Rachakonda, Manjula Alla, Sudha Sravanti
Kotapalli, Ramesh Ummanni, Ramakrishna Munnaluri, Yamini Lingala, Vijjulatha
Manga.
Results

67. Chapter-IV
(Bromodimethylsulfonium)bromide Catalyzed Organic
Transformations Under Solvent Free Conditions

68. Figure 1: Catalytic and chemical nature of BDMS

69. Figure 5. BDMS catalyzed solvent free synthesis of imidazo[1,2-a]pyridines and quinoline

71. Section-A
(Bromodimethylsulfonium)bromide catalyzed solvent free one pot three
component synthesis of imidazo[1,2-a]pyridine

72. Hantzsch
synthesisBucherer-Bergs
reaction
isocyanide based
multicomponent
reactions
Figure 2: Pictorial representation of various multicomoponent reactions

73. Figure 3: Pictorial representation of isonitrile based MCRs

74. Figure 4: Pictorial representation of Strecker, Blackburn and Hulme
imidazo[1,2-a]pyridine synthesis.

75. Scheme 11: BDMS catalyzed synthesis of N-benzylidene-2-phenylimidazo[1,2-a]pyridine derivatives
S.No Addition Sequence Catalyst Solvent Tempa Timeb Yieldc
1 1 9a (1eq) BDMS 7 MeOH r.t 8 41
2 1 9a (1eq) BDMS 7 …….. r.t 8 40
3 1 9a (2eq) BDMS 7 MeOH r.t 8 93
4 1 9a (2eq) BDMS 7 …….. r.t 8 91
5 Schiff’s base of 4-chlorobenzaldehyde, 2-amino
pyridine and catalyst then TMSCN (1:2:1)
…….. r.t 3 91
6 4-chlorobenzaldehyde (2eq), TMSCN and BDMS
stirred for 3 hrs then 2-aminopyridine.
…….. r.t 8 90
Tempa=reaction temparature;Timeb= reaction time in hrs; Yieldc= isolated yield in %.
The above experiments indicate that, both imine and cyanohydrin formation are plausible pathways
for this reaction

77. Plausible mechanism
via imine formation
via cyanohydrin formation

78. Comp Product Time (hrs) Yield (%) Mp (OC) Comp Product Time (hrs) Yield (%) Mp (OC)
10a 10 91 170-174 10i 10 65 182-185
10b 11 70 145-148 10j 10 80 125-128
10c 11 60 93-95 10k 10.5 82 95-98
10d 10 79 184-190 10l
11
72 205-210
10e 10 60 88-90 10m 11 68 173-175
10f 11 70 105-108 1on 11 50 278-281
10g 11 50 200-205 10o 11 55 200- 203
10h 10.5 61 244-247

79. Scheme 12: Hydrolysis of N-(4-chlorobenzylidene)-2-(4-chlorophenyl)imidazo[1,2-a]pyridin-3-amine

80. Section-A
(Bromodimethylsulfonium)bromide catalyzed solvent free Friedlander synthesis of
Quinoline

81. Scheme 14: Friedländer synthesis of quinoline derivatives
S.No BDMSa Yield (%)b Timec
1 0 Traces 24 hrs
2 5 50 2 hrs
3 10 80 50 min
4 20 80 50 min
a; BDMS mol%, b; isolated yield; c; reaction time

82. Figure 7. Plausible mechanism of BDMS catalyzed quinoline synthesis

83. Compound Product Yield (%) M.p (OC) Compound Product Yield (%) M.p (OC)
14a 80 98-100 14l 62 Yellow Oil
14b 81 114-116 14m 83 Yellow Oil
14c 81 105-107 14n 84 178-180
14d 80 153-156 14o 92 130-132
14e 85 94-95 14p 72 217-220
14f 81 196-198 14q 93 144-148
14g 64 116-118 14r 81 315-318
14h 40 90-92 14s 88 136-138
14i 32 74-76 14t 80 154-157
14j 41 188-191 14u 60 Yellow Oil
14k 81 Yellow Oil

85. Total 129 compounds were synthesized. Design, diversity oriented synthesis of
bedaquiline related N-heterocycles were succesfully achieved and evaluated against
M.smegmatis strain for anti TB activity. Based on hits observed, lead generation, SAR and
structure optimization was carried out. In all 100 novel quinolinyl heterocycles, 80
compounds were tested for antimycobacterial activity 34 active and 6 promising
compounds were observed. Low cytotoxocity of the compounds highlighted their
efficiency. In addition to this, these compounds also having good antimicrobial as well as
anti-inflammatiry activities. Synthesis of imidazo[1,2-a]pyridine derivatives (16
compounds) were completely studied and a variety of cyclic, acyclic quinolinyl derivatives
(21 compounds) were achieved under solvent free conditions without involving tedious
purification techniques
Conclusions
Ihe End
But journey will
cotinue…………

86. Acknowledgemen
ts• Dr. A. Manjula, Principal Scientist, Supervisor, CPC Division
CSIR-IICT
• Dr. B. Vittal Rao, Retired Scientist, CPC Division CSIR-IICT
• Head CPC and DIICT
• Proff. V. Ravinder, Head, Dept of Chemistry, KU
• Proff. G. Dayakar, Chairmen, BOS, Dept of Chemistry, KU
• Proff Sadanandam, Dean Faculty of Science
• Teaching Staff Kakatiya University
• Proff. K. Rajareddy, KITS Warangal
• Proff. Komal Reddy, Dean Faculty of Science, Satavahana University, Karimnagar
• Proff. M. Thirumala Chary, Chairmen, BOS, JNTU Hyderabad
• Dr. Madhukar Reddy, KITS Warangal
• Dr. Ramesh Babu, KITS Warangal
• Dr. D. Prabhakara Chary, KITS Warangal
• Dr. Ranadeer Kumar, KITS Warangal
• S. Ramakrishna Reddy (Degree Lecturer)
Friends
& My Family

87. Thank
You