Hair Follicle Stem Cells and Small Intestinal    Submucosa in Vascular engineeringHaofan Peng (Eric) 1, Evan Schlaich 1, S...
Biocompatibility of SIS to stem cells                                     24hr           2 weeks                          ...
HF-SMCs attach and align on SIS                                                SEM                       EGFP   A         ...
Cells proliferation and migration in SIS        No cells                     HF-SMC/SISA                                B ...
SIS constructs show vascular contractility                         A       U46619                        B                ...
3D vascular graft      A                          BFibrin-glued    SIS      C                          DCarotidartery     ...
High burst pressure for arterial implantation                                        Burst pressure                       ...
Bioreactor mimicking arterial enviroment                                                 Chamber                          ...
Endothelial attached on SIS and aligned in              the direction of flow          static                             ...
Ovine carotid arterio-venous shunt model      •Whole blood      •Physiological shear      •Physiological pressure      •Co...
Bioreactor-animal  “hybrid”  modelA                                      B                                            COM ...
Cell seeded grafts have anti-coagulation potential                 Gross images                       H&E           A     ...
Anti-platelet adhesion property               20x              63x                       20x               63x           A...
A       1000x       B           3000x                                  G     1000x         H           3000x              ...
Compliance:Pulse wave velocity & Augmentation index                     1.   Clinical stiffness measurement               ...
SIS grafts have similar compliance as artery      A                                                        B              ...
Summary                             Anti-platelet             FunctionalHF-SMC                                Compliant + ...
Acknowledgement NIH NYSTEM New York state Center of Excellence Buffalo children and women hospital Cook Biotechnology...
Vein   A                                                pressure                                                  pressure...
SIS D                                                                            E                   50                pre...
Artery  G                                                                         H                                       ...
What professors want?   Multiple conditions in parallel   Mimic in vivo environment   Ready to use   Not only for tiss...
What students want?• Computer-driven and monitored• User-friendly• Contamination free Medium change Vessel touch-free B...
Nächste SlideShare
Wird geladen in …5
×

1105 Stem Cells Interaction With Biomaterial And Preclinical Cardiovascular Animal Model

227 Aufrufe

Veröffentlicht am

0 Kommentare
0 Gefällt mir
Statistik
Notizen
  • Als Erste(r) kommentieren

  • Gehören Sie zu den Ersten, denen das gefällt!

Keine Downloads
Aufrufe
Aufrufe insgesamt
227
Auf SlideShare
0
Aus Einbettungen
0
Anzahl an Einbettungen
40
Aktionen
Geteilt
0
Downloads
0
Kommentare
0
Gefällt mir
0
Einbettungen 0
Keine Einbettungen

Keine Notizen für die Folie

1105 Stem Cells Interaction With Biomaterial And Preclinical Cardiovascular Animal Model

  1. 1. Hair Follicle Stem Cells and Small Intestinal Submucosa in Vascular engineeringHaofan Peng (Eric) 1, Evan Schlaich 1, Sindhu Row 1, Daniel Swartz 2, 3 and Stelios Andreadis 1, 3 1 Department of Chemical and Biological Engineering, 2 Department of Pediatrics, Women and Children’s Hospital of Buffalo,3 Center of Excellence in Bioinformatics and Life Sciences, University at Buffalo, State University of New York, Amherst, NY 14260-4200
  2. 2. Biocompatibility of SIS to stem cells 24hr 2 weeks B C A Unstrained PDMS cell D ESIS 10% strain force 1cm force
  3. 3. HF-SMCs attach and align on SIS SEM EGFP A B A C Unstrained 10m 6m 20m 50m D E F10% strain force HF-SMC: Hair follicle derived smooth muscle cells
  4. 4. Cells proliferation and migration in SIS No cells HF-SMC/SISA B H&EC D CollagenE F Elastin 100m
  5. 5. SIS constructs show vascular contractility A U46619 B 2wks 2wks Y27632 24hrs 24hrs C Constriction D Relaxation SIS 24hrs SIS 2wks native artery SIS 24hrs SIS 2wks native artery 200 1.E+05 * * * * * 1.E+04 150Constriction (Pa) Relaxation (%) * 1.E+03 100 1.E+02 50 1.E+01 1.E+00 0 U4 ET1 KCl SNAP Y2
  6. 6. 3D vascular graft A BFibrin-glued SIS C DCarotidartery 1cm 1cm E F 200um Pavcnik et al
  7. 7. High burst pressure for arterial implantation Burst pressure 1400 1200 Burst pressure (mmHg) 1000 800 600 * * 400 200 0 5.5 11 22 Fibrin concentration (mg/ml)
  8. 8. Bioreactor mimicking arterial enviroment Chamber Pressure medium transducerShear stress Rotor1 Rotor 2 Lower Blood Pressure.com Pressure (mmHg) Flow rate (ml/min) 120 450 80 time
  9. 9. Endothelial attached on SIS and aligned in the direction of flow static shear ~1 weekA 4hr B 24hr D flow C 63X E 20m shear
  10. 10. Ovine carotid arterio-venous shunt model •Whole blood •Physiological shear •Physiological pressure •Convenient
  11. 11. Bioreactor-animal  “hybrid”  modelA B COM LED C V FT R PT A LED S:Sheep A: carotid artery PT: Pressure Transducer FT: Flow rate Transducer C:Chamber (grafts) R: Restrictor Com: Computer and digital reader LED: LED micrometer V: jugular vein
  12. 12. Cell seeded grafts have anti-coagulation potential Gross images H&E A D F Istatic static vein vein B E G Jshear shear artery artery 50m C HSIS only SIS only 1cm
  13. 13. Anti-platelet adhesion property 20x 63x 20x 63x A B G H Pstatic vein P C D P I J P arteryshear E FSIS only P 50m 20m
  14. 14. A 1000x B 3000x G 1000x H 3000x p pstatic vein p p p C D I J W p pshear artery p p 10m 50m E F K 70 R p # 60SIS only platelet coverage (%) F 50 # 40 30 20 * 10 * * * 0 SIS static shear artery vein
  15. 15. Compliance:Pulse wave velocity & Augmentation index 1. Clinical stiffness measurement 2. None-invasive 3. Aging, smoking related 4. Stenosis, calcification, hypertension…
  16. 16. SIS grafts have similar compliance as artery A B 40 15 * Augmentation index (%) Pulse wave velocity (m/s) 30 10 20 5 * 10 * 0 0 C 140 Vein SIS Artery Vein SIS Artery 120 *elastic modulus (mmHg) 100 80 60 Important to remodeling: 40 Hyperplasia , stenosis, aneurysm…etc 20 * 0 Vein SIS Artery
  17. 17. Summary Anti-platelet FunctionalHF-SMC Compliant + High Pressure Drugs 3DSIS O O 2 months O O
  18. 18. Acknowledgement NIH NYSTEM New York state Center of Excellence Buffalo children and women hospital Cook Biotechnology Tissue growth technology University of buffalo microscopy center SEM instrument center director
  19. 19. Vein A pressure pressure 50 B 40 Ps Ps Pressure (mmHg) 40 39.5 Pi Pressure (mmHg) 30 A 39 20 Pd 10 38.5 0 38 0 0.5 1 1.5 2 0.45 0.55 0.65 Time (Sec) Time (Sec) C diameter 11.6 distance distance( PT - LED) PWV  Diameter (mm) time TA  TA 11.4 Ps - Pi AIx  Ps - Pd 11.2 A’ 11 0 0.5 1 1.5 2 Time (Sec)
  20. 20. SIS D E 50 pressure 40 pressure Pressure (mmHg) Pressure (mmHg) 40 Ps 30 35 A Pi 20 10 30 0 0.5 1 1.5 2 3.5 4 Time (Sec) Time (Sec) F diameter 6.3Diameter (mm) A’ 6.2 0 0.5 1 1.5 2 Time (Sec)
  21. 21. Artery G H pressure pressure 60 50 Ps 50 Pressure (mmHg) Pressure (mmHg) 45 40 30 A 40 20 Pi 10 35 0 0.5 1 1.5 2 0.1 0.6 Time (Sec) Time (Sec) I diameter 4.35Diameter (mm) A’ 4.3 0 0.5 1 1.5 2 Time (Sec)
  22. 22. What professors want? Multiple conditions in parallel Mimic in vivo environment Ready to use Not only for tissue but for cells too Cheap
  23. 23. What students want?• Computer-driven and monitored• User-friendly• Contamination free Medium change Vessel touch-free Bubbling Noise Leak from vessel Real time reactivity

×