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Highlights on the application of stem cell therapy as the future medicine 
Yuyus Kusnadi 
Stem cell and Cancer Institute 
...
Outline 
Pekan Ilmiah Tahunan VII IDI kota Bogor 
•Sources of stem cells 
•Critical point to prepare stem cells 
•Paracrin...
Sources of stem cells? 
Pekan Ilmiah Tahunan VII 
IDI Kota Bogor
Sources of stem cells 
Bone marrow 
Peripheral blood 
Umbilical cord blood 
Adipose tissue 
Wharton’s jelly 
Placent...
Transplantation 
Pekan Ilmiah Tahunan VII IDI kota Bogor•Autologous•AllogeneicDonor & Recipient
J. Cell. Physiol. 213: 341–347, 2007 
Pekan Ilmiah Tahunan VII IDI kota Bogor
Stem cell from adpose tissue 
Pekan Ilmiah Tahunan VII IDI kota Bogor
Neurogenesis 
Chondrogenesis 
Osteogenesis 
Pekan Ilmiah Tahunan VII IDI kota Bogor
Embryonic stem cells 
Embryonic Stem Cells are taken from the Inner Cell Mass at the Blastocyst stage and cultured in a pe...
Pekan Ilmiah Tahunan VII IDI kota Bogor
•The stem cells were derived from embryos produced in IVF clinics for reproductive purpose. 
NIH guidelines for ESC 
•The ...
Pekan Ilmiah Tahunan VII IDI kota Bogor
Induced pluripotent stem cells 
Pekan Ilmiah Tahunan VII IDI kota Bogor
•Mononuclear cell 
Type of stem cell will be used 
•Hematopoietic stem cell 
•Mesenchymal stem cell 
•Differentiated stem ...
Critical points to prepare stem cell 
Pekan Ilmiah Tahunan VII 
IDI Kota Bogor
Facilities 
•Class D 
•Class C (preparation) 
•Class B (processing) 
Permenkes 50 , 2012 
Pekan Ilmiah Tahunan VII IDI kot...
Materials & reagent 
Active material 
In process control 
Final product 
Quality Control Process 
Pekan Ilmiah Tahunan VII...
Quality control product 
•Sterility test 
•Mycoplasma testing 
•Endotoxin testing 
•Characterization 
•Karyotyping 
•Diffe...
Paracrine Factors 
Pekan Ilmiah Tahunan VII 
IDI Kota Bogor
Is this cell therapy? 
Pekan Ilmiah Tahunan VII IDI kota Bogor
Paracrine factors 
Cytokine & Growth Factor Reviews 20 (2009) 419–427 
MSCs are promising therapeutic tools 
Pekan Ilmiah ...
Paracrine Factors 
Abstract Recent studies suggest that the therapeutic effects of stem cell 
transplantation following my...
Regulation in Indonesia 
Pekan Ilmiah Tahunan VII 
IDI Kota Bogor
Are stem cells regulated? 
•Autologous:Ministry of health 
Permenkes 833, 2009 & Permenkes 50, 2012 
•Allogeneic: National...
Where stem cell can be prepared? 
•inthe hospital 
•outsidethe hospital 
Permenkes 833/834, 2009 
Permenkes 50, 2012 
Peka...
Hospital for stem cell therapy 
•Only teaching hospitals (B class) are allowed to conduct clinical trial of stem cell 
Per...
Issues in clinical study 
Pekan Ilmiah Tahunan VII 
IDI Kota Bogor
Why stem cells are so fascinating? 
Pekan Ilmiah Tahunan VII IDI kota Bogor
Financial 
•Collaboration 
•Having Gran 
•Consumables and reagents are expesive 
Pekan Ilmiah Tahunan VII IDI kota Bogor 
...
Competency of clinician 
•Have ethical approval 
•Recognized by professional associations or medical colleges 
Pekan Ilmia...
Direct tissue injection 
using delivery device 
Current clinical trials of stem cells utilize two delivery 
methodologies:...
•Mononuclear cell 
Type of stem cell will be used 
•Hematopoietic stem cell 
•Mesenchymal stem cell 
•Differentiated stem ...
Summary 
Challenge stem cells as a the future medicine: 
•Source of stem cell will be used 
•Type of stem cells are admini...
Pekan Ilmiah Tahunan VII IDI kota Bogor
Thank you
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Highlights on the application of stem cell therapy as the future medicine yuyus kusnadi

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Highlights on the application of stem cell therapy as the future medicine
Prof. Dr. dr. Yuyus Kusnadi, PhD, MSc
PIT VII IDI Kota Bogor 1-2 November 2014

Veröffentlicht in: Gesundheit & Medizin
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Highlights on the application of stem cell therapy as the future medicine yuyus kusnadi

  1. 1. Highlights on the application of stem cell therapy as the future medicine Yuyus Kusnadi Stem cell and Cancer Institute ReGeniC Laboratory Pekan Ilmiah Tahunan VII IDI Kota Bogor
  2. 2. Outline Pekan Ilmiah Tahunan VII IDI kota Bogor •Sources of stem cells •Critical point to prepare stem cells •Paracrine factors •Regulation in Indonesia •Issues in clinical study
  3. 3. Sources of stem cells? Pekan Ilmiah Tahunan VII IDI Kota Bogor
  4. 4. Sources of stem cells Bone marrow Peripheral blood Umbilical cord blood Adipose tissue Wharton’s jelly Placenta Muscle Amniotic fluid Menstrual blood Dental pulp Etc. Pekan Ilmiah Tahunan VII IDI kota Bogor
  5. 5. Transplantation Pekan Ilmiah Tahunan VII IDI kota Bogor•Autologous•AllogeneicDonor & Recipient
  6. 6. J. Cell. Physiol. 213: 341–347, 2007 Pekan Ilmiah Tahunan VII IDI kota Bogor
  7. 7. Stem cell from adpose tissue Pekan Ilmiah Tahunan VII IDI kota Bogor
  8. 8. Neurogenesis Chondrogenesis Osteogenesis Pekan Ilmiah Tahunan VII IDI kota Bogor
  9. 9. Embryonic stem cells Embryonic Stem Cells are taken from the Inner Cell Mass at the Blastocyst stage and cultured in a petri disc Pekan Ilmiah Tahunan VII IDI kota Bogor
  10. 10. Pekan Ilmiah Tahunan VII IDI kota Bogor
  11. 11. •The stem cells were derived from embryos produced in IVF clinics for reproductive purpose. NIH guidelines for ESC •The stem cells were in excess of clinical need, meaning that donors had achieved a successful pregnancy. •The stem cells were derived from embryos that were frozen. •Informed consent and institutional review board approval was obtained. Pekan Ilmiah Tahunan VII IDI kota Bogor •No exchange of money was made, in order to avoid a financial influence.
  12. 12. Pekan Ilmiah Tahunan VII IDI kota Bogor
  13. 13. Induced pluripotent stem cells Pekan Ilmiah Tahunan VII IDI kota Bogor
  14. 14. •Mononuclear cell Type of stem cell will be used •Hematopoietic stem cell •Mesenchymal stem cell •Differentiated stem cell Pekan Ilmiah Tahunan VII IDI kota Bogor
  15. 15. Critical points to prepare stem cell Pekan Ilmiah Tahunan VII IDI Kota Bogor
  16. 16. Facilities •Class D •Class C (preparation) •Class B (processing) Permenkes 50 , 2012 Pekan Ilmiah Tahunan VII IDI kota Bogor
  17. 17. Materials & reagent Active material In process control Final product Quality Control Process Pekan Ilmiah Tahunan VII IDI kota Bogor
  18. 18. Quality control product •Sterility test •Mycoplasma testing •Endotoxin testing •Characterization •Karyotyping •Differentiation capacity •HLA Pekan Ilmiah Tahunan VII IDI kota Bogor
  19. 19. Paracrine Factors Pekan Ilmiah Tahunan VII IDI Kota Bogor
  20. 20. Is this cell therapy? Pekan Ilmiah Tahunan VII IDI kota Bogor
  21. 21. Paracrine factors Cytokine & Growth Factor Reviews 20 (2009) 419–427 MSCs are promising therapeutic tools Pekan Ilmiah Tahunan VII IDI kota Bogor
  22. 22. Paracrine Factors Abstract Recent studies suggest that the therapeutic effects of stem cell transplantation following myocardial infarction (MI) are mediated by paracrine factors. One of the main goals in the treatment of ischemic heart disease is to stimulate vascular repair mechanisms. Here, we sought to explore the therapeutic angiogenic potential of mesenchymal stem cell (MSC) secretions. Human MSC secretions were collected as conditioned medium (MSC-CM) using a clinically compliant protocol. Pekan Ilmiah Tahunan VII IDI kota Bogor
  23. 23. Regulation in Indonesia Pekan Ilmiah Tahunan VII IDI Kota Bogor
  24. 24. Are stem cells regulated? •Autologous:Ministry of health Permenkes 833, 2009 & Permenkes 50, 2012 •Allogeneic: National Agency for drug and food (Badan POM) as biological product Pekan Ilmiah Tahunan VII IDI kota Bogor
  25. 25. Where stem cell can be prepared? •inthe hospital •outsidethe hospital Permenkes 833/834, 2009 Permenkes 50, 2012 Pekan Ilmiah Tahunan VII IDI kota Bogor
  26. 26. Hospital for stem cell therapy •Only teaching hospitals (B class) are allowed to conduct clinical trial of stem cell Permenkes 833/834, 2009 •M. Jamil •Cipto Mangunkusumo •Harapan Kita •Dharmais •Persahabatan •Fatmawati •Hasan Sadikin •Karyadi •Sardjito •Soetomo •Sanglah •Permenkes 32, 2014, there are 11 hospital in Indonesia are allowed: Pekan Ilmiah Tahunan VII IDI kota Bogor
  27. 27. Issues in clinical study Pekan Ilmiah Tahunan VII IDI Kota Bogor
  28. 28. Why stem cells are so fascinating? Pekan Ilmiah Tahunan VII IDI kota Bogor
  29. 29. Financial •Collaboration •Having Gran •Consumables and reagents are expesive Pekan Ilmiah Tahunan VII IDI kota Bogor •Patient will pay
  30. 30. Competency of clinician •Have ethical approval •Recognized by professional associations or medical colleges Pekan Ilmiah Tahunan VII IDI kota Bogor
  31. 31. Direct tissue injection using delivery device Current clinical trials of stem cells utilize two delivery methodologies: Intravenous Delivery of stem cell to patient Pekan Ilmiah Tahunan VII IDI kota Bogor
  32. 32. •Mononuclear cell Type of stem cell will be used •Hematopoietic stem cell •Mesenchymal stem cell •Differentiated stem cell Pekan Ilmiah Tahunan VII IDI kota Bogor
  33. 33. Summary Challenge stem cells as a the future medicine: •Source of stem cell will be used •Type of stem cells are administered •Delivery system of stem cells to patient •Reduce cost •Therapeutic dose and timing Pekan Ilmiah Tahunan VII IDI kota Bogor
  34. 34. Pekan Ilmiah Tahunan VII IDI kota Bogor
  35. 35. Thank you

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