1. Tech Challenge
Bio.Kitchen
Challenges
1. Teach COVID a Lesson: AR guided COVID-19 Testing in a mobile Lab
2. Bio.Game: Build Tools to fast forward Evolution
23.04.2020

2. ootstrapping im Bio/Life-Scince Bereich
“If I were a teenager today, I'd be hacking biology.”
Bill Gates
Bootstrapping
biological innovation

3. Bio.Kitchen enables lean and agile development of life science innovation
Biological innovation to date is not
agile. Large investments and time is
required to develop new prototypes
until market readiness. Our mobile
Bio.Kitchen brings agile innovation
into the domains of chemistry,
biology, pharma and medicine.
In our courses we merge the
organic, technical and digital worlds
to generate sustainable generation
of value following the UN “one
health” concept, to give answers to
the challenge of the 21. century:
green economy, food and
healthcare.
Agility: We analyze technology, user
needs and viability in an integrated and
flexible process
Transdisciplinarity: We work across
disciplines
High-Tech Hacking: We hack and
recombine technologies creatively
Rapid Prototyping: we can quickly
iterate and test biological, hardware and
software prototypes in less than a week
Neutral Ground: We dont take any
equity in our users inventions and open
source our own technology
Founder’s Mindset: We enable holistic
education and personality development.

4. Bio.Kitchen is an open community lab for tech-talents and life science innovation
our workstations: Lab.Bio - Micro.Bio - Syn.Bio - Play.Bio
our service: biopreneur community - biotech hacking - open laboratory - educational courses
Rüdiger Trojok
Lab Lead & Biohacker
Leo Sprengel
Lab Assistant
Dr. Dominik Böhler
Head of Entrepreneurship
& Tech Education

5. with our own mobile Tools & Tech we teach rapid prototyping & agile Innovation
Open Source lab
technology
High-Tech Analytics
Lab on a Chip Chemistry
and Biology
open reagents collection
We lower administrative, logistic and regulatory
hurdles to enable agile innovation in the life
sciences. Our competent team gives practical
workshops and provides a full equipped mobile lab
infrastructure for chemistry, biology, medtech and
material sciences. Participants In our workshops will
be guided from idea to a functional prototype and
then receive further coaching on how to form a
startup and commercialize their ideas and
technologies.

7. Biocommons
Synbio Oath
Read & Write DNA
printing DNA
Biological
Transformation
cradle to cradle
economics
Personalised
Medicine
phage therapy
lectures
Play.Bio
SLA 3D printing
Lego Biochips
Future lab
Bio-Computer
workshop
Biohacking
pharma bioethics
seminar
biological rapid
prototyping
Syn.bio
cell free system
workshop
Biosensors
station
Internet of Living
Things workshop
Lab.Bio
fungi cultivation
plant cultivation
insect cultivation
Microbiology for
beginners
Micro.Bio
sequencing DNA
Bio.Kitchen
lab intro workshop
yeast cultivation
open source
open licensing of
high tech
Biotic games
bio tournament
Biohacking
workshops
hacking evolution
workshop
prototype nature
biodesign studies
Microfluidics
DIY automation
workshop
sci-tech
concepts
education format
bootstrapping
biotech startups
personal phage
therapy
synthetic phage
workshop
advanced
Microbiology
Bio-Playstation
workshop
Copying DNA
workshop
one health
strategy
interplanetary
Bioteleportation
bacteria
cultivation
phage cultivation
We offer a large range of technology workshops, materials and tools

8. Micro.bio Syn.bio mini & max Play.bio Lab.bio
Safely grow, identify and select
microorganisms using a mobile
incubator and autoclave.
Expression of transgenic proteins
is possible with cell free systems.
Syn.bio mini provides DNA analytic
hardware. The Syn.Bio max station
provides the Kilobaser and
Nanopore technologies for
sequencing and synthesis of DNA
of DNA and comes with a
computer to program and analyze
DNA with a QPCR and our
desktop tools.
Our microfluidic toolbox enables
you to 3D print microfluidic
devices. Combine detectors for
optical density measurement with
actuators like pumps and valves
and our open source fluidic bricks
to rapid prototype your own
Bio.Tournament setup.
The station is equipped with a
centrifuge, pipettes, a
transportable fridge and freezer to
keep your reagents fresh and
usable anywhere. The convenient
storage of consumables and the
safe transportation of chemicals
make it the ideal mobile lab
platform.
Our Bio.Kitchen Mobile Lab covers the most relevant processes for early-stage
biological rapid prototyping within four work areas.

9. Our Bio.Kitchen Mobile Lab is a lab-in-a-box for events, education and innovation
We developed four mobile laboratory stations: Lab.bio (comprised of 2 units), Micro.bio, Syn.bio and Play.bio, which
can be easily equipped, transported and operated with minimal logistic effort. The mobile lab is ready to use within
24h, anywhere you want it to be. The full range of our courses can be offered at any preferred location. The lab
storage solution is optimized for our equipment and courses (see previous slide) and is size compatible with
Eurocontainers. Extensions of the table area can be added and stored conveniently for transportation. We offer new
demonstration experiments and the Bio.Tournament program. Of course, the lab itself can be used independently as a
technology support unit for any desired application, too.

10. COVID-19 Dance - North Africa lacks testing capacity
and needs a simple test protocol.
COVID-19 Dance consortium is building solution and
independent supply chain for COVID-19 Testing in mobile
labs using PT-qPCR and isothermal amplification.
Consortial Lead:
Concept Development
and Project Management
Production and
manufacturing of
mobile lab hardware.
OxGEn produces RNA
extraction reactions and
gets validation by
Georgian CDC
Clinical pilot with
diagnostic labs in May
Pilot study to establish additional test capacity
- Production of COVID-19 isothermal amplification
- 30 mobile lab stations
- 1.6 Million Tests in 6 months pilot study
- Scalable technology for continental rollout
- Sustainable reuse of technology after pandemic
Patient
sample
Virus
RNA
Rapid Virus RNA
extraction ~30min
Isothermal Ampflication
~30min
Reagents
1h
Patient
Diagnosis
Northern Africa needs an immense increase in test
capacity to fight off potential effects of COVID-19
Mai 2020 - Continuous improvement, field testing and education of healthcare professionals - December 2021
TUM produces a batch
of isothermal
amplification kits.

11. UnternehmerTUM GmbH / 21.11.19
AP5: Skalierung und Betrieb vor Ort
AP4: Produktion von Test-Kits
AP1: Produktion mobiler Labore
AP2: Ausbildung von Trainern
8
200
~35€
30
300
25-30€
30
800
<25€
# Labore
Tests/Tag und Labor
Kosten/Test
In 3 Phasen können wir Testkapazität für bis zu
1.6 Millionen Tests bei Vollkosten <25€ pro Test schaffen.
● Bau von 8 Stationen
● Zukauf von Reagenzien und
Maschinen für qPCR-Verfahren
● Probebetrieb von 8 mobilen Laboren vor Ort
● Erprobung Logistik
April-Mai ‘20
Prototyping
Juni-Juli ‘20
Testbetrieb, Produktion Reagenzien
August-Dezember ‘20
Skalierung Betrieb vor Ort
Bau
und
Betrieb
Labore
Entwicklung
und
Produktion
Reagenzien
KPIs
AP3: Entwicklung und Validierung von vereinfachten Testverfahren:
● Optimierung oder Effizienzsteigerung der isothermalen Amplifikationsreaktion
● Klinische Vorstudie am MRI
● Selektion eines Produktionspartners für
Test-Kits
● Schrittweise Skalierung auf 1.5 Millionen
Kits
● Ausbildung von 12 Trainern
● Ausbildung von weiteren 24 Trainern
● Entwicklung Online-Content
● Produktion von qPCR Testkits
mit OxGen, Tiflis, Georgien
● Bau von weiteren 22 Stationen
● Skalierung der isothermalen Reaktion
● Remoteunterstützung bei Bedarf
● Optimierung der Testkits auf den
Einsatz in Nordafrika auf Basis der
Forschungsergebnisse
● Kontinuierliche Produktion und
Logistik

12. Enabling Corona Testing Stations

13. UnternehmerTUM GmbH / 21.11.19
Phase 1: qPCR test protocol give
high quality results in 2h in the mobile lab
https://www.chaibio.com/openqpcr
0. Sample collection by
medical staff.
The patient sample is taken
with a swab, packaged and
labelled. Patient data is
stored with a smartphone.
1. Virus RNA extraction
virus lysis and RNA
purification using
established protocols with
a centrifuge in 1h. QR code
labels are used throughout
the process.
2. QPCR RNA
amplification
is done with a reverse
transcription and QPCR
protocol and CE certified
molecular biology test kits
in 2h.
3. machine result readout
is ready after 2h. QR codes
link the results to the
patient data.
Documentation happens by
smartphone photo and is
stored offline.

14. UnternehmerTUM GmbH / 21.11.19
Phase 2: quick-pre-test protocol give results in 1h
without the need for thermocyclers or centrifuges
https://international.neb.com/protocols/2016/08/15/warmstart-colorimetric-lamp-2x-master-mix-typical-lamp-protocol-m1800
http://oxgen.ge/product/pcr-fragment-purification/
0. Sample collection by
medical staff.
The patient sample is taken
with a swab, packaged and
labelled. Patient data is
stored with a smartphone.
1. Virus RNA extraction
using a simplified virus lysis
and RNA purification
protocol in 30 mins. QR
code labels are used
throughout the process.
2. QPCR RNA
amplification
is done with a reverse
transcription and TUM
certified isothermal LAMP
molecular biology test kits
in 30mins.
3. machine result readout
is ready after 1h with
minimal hands on time. QR
codes link the results to
the patient data.
Documentation happens by
smartphone photo and is
stored offline. Positive
results should be
confirmed with QPCR

15. UnternehmerTUM GmbH / 21.11.19
Phase 3: quick-pre-test protocol give results in 20mins
without the need for thermocyclers or centrifuges
https://international.neb.com/protocols/2016/08/15/warmstart-colorimetric-lamp-2x-master-mix-typical-lamp-protocol-m1800
http://oxgen.ge/product/pcr-fragment-purification/
0. Sample collection by medical staff
The patient sample is taken with a
swab, packaged and labelled. Patient
data is stored with a smartphone.
1. Isothermal RNA amplification
is done with LAMP molecular biology
test kit in a manual but high
throughput method.
2. visual result detection
is ready after 1h for photo
documentation with minimal hands
on time and lab equipment. Positive
results should be confirmed with
qPCR.

16. UnternehmerTUM GmbH / 21.11.19
Phase 4: automated quick-pre-test protocol give results
with only 5 mins hands on time
https://international.neb.com/protocols/2016/08/15/warmstart-colorimetric-lamp-2x-master-mix-typical-lamp-protocol-m1800
http://oxgen.ge/product/pcr-fragment-purification/
0. Sample collection by medical staff
The patient sample is taken with a
swab, packaged and labelled. Patient
data is stored by any state approved
smartphone app for Corona pandemic
control.
1. automated Isothermal RNA
amplification
is done with LAMP molecular biology
test kit in a automated and high
throughput method. No further lab
equipment needed.
2. visual result detection
is ready after 1h with 5mins hands on
time. Results are automatically
uploaded to any Corona pandemic
control app.

17. UnternehmerTUM GmbH / 21.11.19
Micro.bio Lab.bio
Safely isolate and extract coronaviruses using a mobile
glovebox with underpressure and autoclave to sterilize
contaminated materials. The station is designed for COVID-19
RNA extraction and comes with a RNA extraction kit supply
by Oxgen company. The station turns any tent, airport, or
hospital room or any place you need into a fully functional
biosafety level 2 facility.
The station provides a heat block, pipettes, a fridge and
freezer to keep your reagents fresh. With a heatblock, the
isothermal reaction can be controlled in temperature and
duration. Diagnostic results are documented by a foto with
the users smartphone. The convenient storage of
consumables and the safe transportation of chemicals
make it the ideal mobile lab platform for any environment.
Our Bio.Kitchen Mobile Lab covers all processes for
COVID-19 detection and safe sample handling
UnternehmerTUM GmbH / 21.11.19
17
https://www.chaibio.com/openqpcr
http://www.glovebox-systemtechnik.de/GS-Kunststoff-Glovebox.php
all pictures are for illustration only. The final product may differ.

18. Our Bio.Kitchen Mobile Corona Diagnostic Lab is a
ruggedized lab-in-a-box for pandemic response
▪ 3 mobile lab stations: Lab.bio (2 units), Micro.bio, Syn.bio (optional)
▪ Easily transported and operation - ready to use within ~24h, anywhere in EU
▪ Compatible to our molecular and microbiological lab tools
▪ Optimized equipment storage compatible with Eurocontainers
▪ Extendable table top area to a maximum of 7.2 m² (5 work spaces)
▪ On demand: mobile energy generator and voltage regulators

19. UnternehmerTUM GmbH / 21.11.19
With 1 station per 100 000 citizens our mobile lab allows
to compensate the lack of testing capacity in Germany.
Assumptions:
● Staffing: 3 clinical laboratory technician working in shifts 24/7
● 36 samples handled per hour
● with QPCR testing hands on time 120 mins to result
● isothermal testing hands on time 60 mins to result
● with automation the diagnoses per day can be scaled significantly
Maximum manual capacity in Phase 3:
800 diagnoses per station per 24h

20. Play.Bio
Bio.Game Environmental Challenge - evolve your organisms to live in the
microplastic habitat
Bio.Game teams develop biotechnological innovations and playfully
compete with each other by growing their organisms to explore a
new habitat on a petri dish. Participants learn our unique rapid
prototyping methodology to hack ecology and evolution to outsmart
your opponent bacterial cultures. By building their own open source
lab-on-a-chip “bio playstation” teams can test their ideas in a
real-time biological competition against other teams. The Bio.Game
can be hosted using our Micro.Bio and Play.Bio mobile lab stations.
Help us to develop this biological gaming station.
Lab.bio
● Evolve
● Select
● Expression
Micro.Bio
● Cultivate
● Optimize
● Diversify
Play.bio
● Automate
● Miniaturise
● Scale up
play.bio

21. 21
Challenge 2: Bio-Tournament
21
A biological competition.
Hack evolution and outsmart your
opponent bacterial cultures.
Advance your microfluidic workstation
and overcome the opponents cells with
phages and resistance breeding.
Apply your setup in a real-time biological
competition to take over as much space
on the agar playground as possible.

22. Moore’s Law for Biology
Exponentially grow the number of experiments per scientist
Experiments
per day
10.000.000
1.000.000
100.000
1.000
100
2009 2012 2015 2018 2021 2024

23. 23

24. Bio.Kitchen prices and offers
▪ take part in online and lab courses
- mobile lab construction workshop
- Micro-bio for beginners course
- Corona diagnosis course
- Rapid Prototyping Lab Automation course
▪ use our open innovation lab during this semester for free every monday afternoon
▪ receive free reagents and play the Bio.Game against the Bio.Kitchen team
▪ be a part in the first trial run of the mobile COVID-19 test lab
Join our Deep Dive on Wednesday 1pm - 3pm on Zoom to get more information or contact me on Slack any time.
Come by on monday afternoon to our Laboratory in:
UnternehmerTUM GmbH
Lichtenbergstr. 6
85748 Garching (Munich), Germany

25. Vielen Dank!
UnternehmerTUM GmbH
Lichtenbergstrasse 6
85748 Garching (Munich), Germany
Tel.+49 (89) 18 94 69-0
www.unternehmertum.de
UnternehmerTUM GmbH
Lichtenbergstr. 6
85748 Garching (Munich), Germany
+49-178-8835667
+995 598 26 67 61
trojok@unternehmertum.de
Rüdiger Trojok
Lab Lead & Biohacker