SlideShare ist ein Scribd-Unternehmen logo
1 von 51
Downloaden Sie, um offline zu lesen
Prymnesium parvum : Analysis of Pigmentation Dynamics Over Growth  by Alexis Martinez Identification through Bio-Optical Signatures  by Merissa Ludwig   REU Program Texas A&M University Summer 2005
Golden Algae ,[object Object],[object Object],         Photo: Dr. Carmelo Tomas UNC Wilmington
[object Object],[object Object],[object Object],[object Object]
Golden Algae Family Tree Illustrations by Robert G. Howells, TPWD
Cell Characteristics ,[object Object],[object Object],[object Object],[object Object],[object Object],                                Golden Alga,  Prymnesium parvum Drawing by Robert G. Howells, TPWD
“ Golden” Algae? ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],Ideal Environment for  P. parvum Photo: Dave Buzan, TPWD
What makes this algae so important? ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Unique Characteristics ,[object Object],[object Object],[object Object],[object Object],Photos by Urban Tillmann TIllmann, U. (1998) AME 14: 155-160
Where can we find golden algae? ,[object Object],[object Object],[object Object]
 
Locations found in the U.S. ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
 
History of  P. parvum  in Texas ,[object Object],[object Object],[object Object]
Impacts of Blooms ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Fish kill at Jackson Bend, Lake Granbury, 2003
Problem Research Solution Persistent toxic algae with the ability to decimate fish,  bivalve, and other phytoplankton populations.  Texas  economy feels effect in declining tourism and recreation  due to dead fish. Knowledge about  P. parvum  is limited, inability to detect indicators prior to harmful algal bloom. Immediate need for simple, rapid, and more accurate method to detect  P. parvum and to identify possible indicators before harmful algal bloom.
Current Methods of Identification   ,[object Object],[object Object],[object Object],Light micrograph (phase contrast) of a water sample, containing predominantly Prymnesium parvum , collected from Lake Koronia on 11th September, 2004. Inset: Motile  P. parvum  cell with visible flagellae and haptonema. Bars indicate 10 μm; inset 5 μm.
Future Methods ,[object Object],[object Object],[object Object],[object Object]
Experiment ,[object Object],[object Object],[object Object]
Experiment ,[object Object],[object Object],[object Object],[object Object],[object Object]
 
Prymnesium   parvum  Growth Characteristics:  Analysis of Pigmentation with Cell Growth ,[object Object]
Method for Assessing   Phytoplankton Assemblages ,[object Object],[object Object],[object Object],[object Object]
Pigments ,[object Object],[object Object],[object Object],[object Object]
Pigments ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],*Specific algal groups contain diagnostic pigments
High-Performance Liquid Chromatography   Fig. 1. Preparative and analytical procedures for HPLC from Millie et. al. (1993).
Chemical Taxonomy ,[object Object],[object Object],ChemTax   matrix software program = Algal group abundance
Objective of Study ,[object Object],[object Object],[object Object]
Analysis of Growth Characteristics: ,[object Object],[object Object]
Results
 
 
A B C
Pigment ratio statistics of entire sampling period (Day 3 – 24) 57 30 38 18 24 15 COV %   0.01 0.06 0.01 0.09 0.04 0.01 STDV   0.03 0.20 0.01 0.48 0.15 0.05 AVG C     39 24 53 21 23 27 COV %   0.01 0.05 0.01 0.11 0.04 0.02 STDV   0.03 0.23 0.01 0.53 0.18 0.06 AVG B     56 13 57 12 21 33 COV %   0.01 0.03 0.01 0.06 0.03 0.02 STDV   0.02 0.19 0.01 0.49 0.16 0.05 AVG A Zeax- Diado-/Diato Viola- Fuco- Chl c1c2 Chl c3 TOTAL  
Growth Phase  Pigmentation Dynamics (highlighted areas display very high correlation of coefficient values) 86 23 40 14 21 16 COV %   0.01 0.04 0.01 0.07 0.03 0.01 STDV   0.02 0.16 0.01 0.47 0.13 0.05 AVG C     56 28 82 25 31 39 COV %   0.01 0.06 0.01 0.14 0.05 0.02 STDV   0.02 0.21 0.01 0.55 0.16 0.06 AVG B     74 10 90 10 30 42 COV %   0.01 0.02 0.01 0.05 0.05 0.02 STDV   0.01 0.17 0.01 0.50 0.15 0.06 AVG A Zeax- Diado-/Diato Viola- Fuco- Chl c1c2 Chl c3 GROWTH  
Stationary Phase  Pigmentation Dynamics 20 0 47 12 27 0 COV %   0.01 0.00 0.01 0.06 0.04 0.00 STDV   0.04 0.23 0.02 0.48 0.16 0.05 AVG C     16 23 0 12 13 17 COV %   0.01 0.06 0.00 0.07 0.03 0.01 STDV   0.04 0.25 0.01 0.55 0.20 0.06 AVG B     29 5 11 13 7 15 COV %   0.01 0.01 0.00 0.07 0.01 0.01 STDV   0.03 0.21 0.01 0.50 0.18 0.05 AVG A Zeax- Diado-/Diato Viola- Fuco- Chl c1c2 Chl c3 STATIONARY  
Comparison of  separating  analysis over phases versus using entire sampling period 57 30 38 18 24 15 TOTAL   20 0 47 12 27 0 STATIONARY    86 23 40 14 21 16 GROWTH  C       39 24 53 21 23 27 TOTAL   16 23 0 12 13 17 STATIONARY   56 28 82 25 31 39 GROWTH B       56 13 57 12 21 33 TOTAL   29 5 11 13 7 15 STATIONARY   74 10 90 10 30 42 GROWTH A Zeax- Diado-/Diato Viola- Fuco- Chl c1c2 Chl c3 COV %  
CONCLUSION ,[object Object],[object Object],[object Object],[object Object]
Rapid Detection through use of Optical Signatures Merissa Ludwig
Bio-Optical Methodologies ,[object Object],[object Object],[object Object],[object Object]
Finding an Optical Signature ,[object Object],[object Object],[object Object],[object Object]
Absorption ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object]
Collecting Data ,[object Object],[object Object],[object Object],[object Object]
Absorbance
Variance Values ,[object Object],[object Object],4.61 480 C 6.18 413 B 5.54 483 A Coefficient of Variation Wavelength (nm) Sample Maximum Variance in Absorbance
Optical Signature and Growth ,[object Object],[object Object]
Optical Signatures and Growth ,[object Object],[object Object],[object Object],[object Object]
Other Projects in the Lab ,[object Object],[object Object],[object Object],[object Object]
 
Acknowledgements ,[object Object],[object Object],[object Object],[object Object],[object Object]

Weitere ähnliche Inhalte

Was ist angesagt?

Economic importance of cyanobacteria
Economic importance of cyanobacteriaEconomic importance of cyanobacteria
Economic importance of cyanobacteriapayal96
 
Vegetative characters of equisetum
Vegetative characters of equisetumVegetative characters of equisetum
Vegetative characters of equisetumSijo A
 
Hutchinsons system of classification
Hutchinsons system of classificationHutchinsons system of classification
Hutchinsons system of classificationDr. T. A. Gitte
 
MASTERS BOTANY PROJECT TOPICS AND MATERIALS.docx
MASTERS BOTANY PROJECT TOPICS AND MATERIALS.docxMASTERS BOTANY PROJECT TOPICS AND MATERIALS.docx
MASTERS BOTANY PROJECT TOPICS AND MATERIALS.docxDanielChukwudi2
 
Economic and Ecological Importance of Bryophytes.pptx
Economic and Ecological Importance of Bryophytes.pptxEconomic and Ecological Importance of Bryophytes.pptx
Economic and Ecological Importance of Bryophytes.pptxDilip Gavande
 
All about Gnetum (Gymnosperm)
All about Gnetum (Gymnosperm)All about Gnetum (Gymnosperm)
All about Gnetum (Gymnosperm)Ratnakar Upadhyay
 
Bryophytes as ecological indicators
Bryophytes as ecological indicatorsBryophytes as ecological indicators
Bryophytes as ecological indicatorsAlen Shaji
 
Gymnosperm and its characteristics
Gymnosperm and its characteristicsGymnosperm and its characteristics
Gymnosperm and its characteristicspankaj kushwaha
 
Gymnosperm characteristics
Gymnosperm characteristics Gymnosperm characteristics
Gymnosperm characteristics Mohd Tariq
 
Economic importance of Bryophytes
Economic importance of BryophytesEconomic importance of Bryophytes
Economic importance of BryophytesSijo A
 

Was ist angesagt? (20)

Economic importance of cyanobacteria
Economic importance of cyanobacteriaEconomic importance of cyanobacteria
Economic importance of cyanobacteria
 
Vegetative characters of equisetum
Vegetative characters of equisetumVegetative characters of equisetum
Vegetative characters of equisetum
 
Hutchinsons system of classification
Hutchinsons system of classificationHutchinsons system of classification
Hutchinsons system of classification
 
Allomyces
AllomycesAllomyces
Allomyces
 
lichen
lichen lichen
lichen
 
Pteridophytes
PteridophytesPteridophytes
Pteridophytes
 
Pentoxylon
PentoxylonPentoxylon
Pentoxylon
 
MASTERS BOTANY PROJECT TOPICS AND MATERIALS.docx
MASTERS BOTANY PROJECT TOPICS AND MATERIALS.docxMASTERS BOTANY PROJECT TOPICS AND MATERIALS.docx
MASTERS BOTANY PROJECT TOPICS AND MATERIALS.docx
 
Gymnosperms
GymnospermsGymnosperms
Gymnosperms
 
Economic and Ecological Importance of Bryophytes.pptx
Economic and Ecological Importance of Bryophytes.pptxEconomic and Ecological Importance of Bryophytes.pptx
Economic and Ecological Importance of Bryophytes.pptx
 
All about Gnetum (Gymnosperm)
All about Gnetum (Gymnosperm)All about Gnetum (Gymnosperm)
All about Gnetum (Gymnosperm)
 
Soral evolution in ferns
Soral evolution in fernsSoral evolution in ferns
Soral evolution in ferns
 
Pellia
PelliaPellia
Pellia
 
Anthoceros.ppt
Anthoceros.pptAnthoceros.ppt
Anthoceros.ppt
 
Flora, Revision and Monograph
Flora, Revision and  MonographFlora, Revision and  Monograph
Flora, Revision and Monograph
 
Bryophytes as ecological indicators
Bryophytes as ecological indicatorsBryophytes as ecological indicators
Bryophytes as ecological indicators
 
Gymnosperm and its characteristics
Gymnosperm and its characteristicsGymnosperm and its characteristics
Gymnosperm and its characteristics
 
Gymnosperm characteristics
Gymnosperm characteristics Gymnosperm characteristics
Gymnosperm characteristics
 
Economic importance of Bryophytes
Economic importance of BryophytesEconomic importance of Bryophytes
Economic importance of Bryophytes
 
Nodal Anatomy
Nodal AnatomyNodal Anatomy
Nodal Anatomy
 

Andere mochten auch (7)

Pterois
PteroisPterois
Pterois
 
Algae seaweeds
Algae  seaweedsAlgae  seaweeds
Algae seaweeds
 
Algae[1]
Algae[1]Algae[1]
Algae[1]
 
Protists
ProtistsProtists
Protists
 
ALGAE (PLANT KINGDOM)
ALGAE (PLANT KINGDOM)ALGAE (PLANT KINGDOM)
ALGAE (PLANT KINGDOM)
 
algae classification
algae classificationalgae classification
algae classification
 
Algae
AlgaeAlgae
Algae
 

Ähnlich wie Golden Algae

Assessment of the Plankton Biodiversity, Bay of Bengal, Cox's Bazar, Bangladesh
Assessment of the Plankton Biodiversity,Bay of Bengal, Cox's Bazar, BangladeshAssessment of the Plankton Biodiversity,Bay of Bengal, Cox's Bazar, Bangladesh
Assessment of the Plankton Biodiversity, Bay of Bengal, Cox's Bazar, BangladeshAbuMusa51
 
Penta aquaticf ate and effects
Penta aquaticf ate and effectsPenta aquaticf ate and effects
Penta aquaticf ate and effectsbann11f
 
321 samocha 9 00- final_go 100 m3
321 samocha 9 00- final_go 100 m3321 samocha 9 00- final_go 100 m3
321 samocha 9 00- final_go 100 m3Pedro Henrique
 
Use of stable and radio isotopes to understand the plant physiological process
Use of stable and radio isotopes to understand the plant physiological processUse of stable and radio isotopes to understand the plant physiological process
Use of stable and radio isotopes to understand the plant physiological processRAHUL GOPALE
 
OCCURRENCE, SEVERITY ASSESSMENT AND MANAGEMENT OF CITRUS GREENING IN KAVREPAL...
OCCURRENCE, SEVERITY ASSESSMENT AND MANAGEMENT OF CITRUS GREENING IN KAVREPAL...OCCURRENCE, SEVERITY ASSESSMENT AND MANAGEMENT OF CITRUS GREENING IN KAVREPAL...
OCCURRENCE, SEVERITY ASSESSMENT AND MANAGEMENT OF CITRUS GREENING IN KAVREPAL...IAAS, TU Nepal
 
Microbiological quality of drinking water
Microbiological quality of drinking water Microbiological quality of drinking water
Microbiological quality of drinking water Mirko Rossi
 
Lab #11 – EcotoxicologyPrelab DiscussionT.docx
Lab #11 – EcotoxicologyPrelab DiscussionT.docxLab #11 – EcotoxicologyPrelab DiscussionT.docx
Lab #11 – EcotoxicologyPrelab DiscussionT.docxcroysierkathey
 
Accumualtion of TBT and Intersex Occurence on Marine Snails Littoraria Scabra
Accumualtion of TBT and Intersex Occurence on Marine Snails Littoraria ScabraAccumualtion of TBT and Intersex Occurence on Marine Snails Littoraria Scabra
Accumualtion of TBT and Intersex Occurence on Marine Snails Littoraria ScabraMarkus T Lasut
 
Accumualtion of TBT and Intersex Occurence on Marine Snails Littoraria Scabra
Accumualtion of TBT and Intersex Occurence on Marine Snails Littoraria ScabraAccumualtion of TBT and Intersex Occurence on Marine Snails Littoraria Scabra
Accumualtion of TBT and Intersex Occurence on Marine Snails Littoraria Scabraguest355941c
 
Accumualtion of TBT and Intersex Occurence on Marine Snails Littoraria Scabra
Accumualtion of TBT and Intersex Occurence on Marine Snails Littoraria ScabraAccumualtion of TBT and Intersex Occurence on Marine Snails Littoraria Scabra
Accumualtion of TBT and Intersex Occurence on Marine Snails Littoraria Scabraguest68e9c2
 
Intro Getting Your Feet Wet: Intro to Different Types of Monitoring
Intro Getting Your Feet Wet: Intro to Different Types of MonitoringIntro Getting Your Feet Wet: Intro to Different Types of Monitoring
Intro Getting Your Feet Wet: Intro to Different Types of MonitoringThe Watershed Institute
 
Selection of Biological Methods for Industrial Effluents Assessments
Selection of Biological Methods for Industrial Effluents AssessmentsSelection of Biological Methods for Industrial Effluents Assessments
Selection of Biological Methods for Industrial Effluents AssessmentsGlobal Risk Forum GRFDavos
 
NJ AWWA Annual Conference
NJ AWWA Annual ConferenceNJ AWWA Annual Conference
NJ AWWA Annual ConferenceMichael Cohrs
 
Monitoring for Harmful Algal Blooms | From Data to Decisions
Monitoring for Harmful Algal Blooms | From Data to DecisionsMonitoring for Harmful Algal Blooms | From Data to Decisions
Monitoring for Harmful Algal Blooms | From Data to DecisionsXylem Inc.
 
Minorisa minuta and the transition to secondary plastid endosymbiosis. A sing...
Minorisa minuta and the transition to secondary plastid endosymbiosis. A sing...Minorisa minuta and the transition to secondary plastid endosymbiosis. A sing...
Minorisa minuta and the transition to secondary plastid endosymbiosis. A sing...Javier del Campo
 

Ähnlich wie Golden Algae (20)

Carotenoids quantification in Cassava
Carotenoids quantification in CassavaCarotenoids quantification in Cassava
Carotenoids quantification in Cassava
 
Assessment of the Plankton Biodiversity, Bay of Bengal, Cox's Bazar, Bangladesh
Assessment of the Plankton Biodiversity,Bay of Bengal, Cox's Bazar, BangladeshAssessment of the Plankton Biodiversity,Bay of Bengal, Cox's Bazar, Bangladesh
Assessment of the Plankton Biodiversity, Bay of Bengal, Cox's Bazar, Bangladesh
 
Rodriguez- HPLC-DAD.pdf
Rodriguez- HPLC-DAD.pdfRodriguez- HPLC-DAD.pdf
Rodriguez- HPLC-DAD.pdf
 
Charleston Harbor Marina Copper Study
Charleston Harbor Marina Copper StudyCharleston Harbor Marina Copper Study
Charleston Harbor Marina Copper Study
 
Penta aquaticf ate and effects
Penta aquaticf ate and effectsPenta aquaticf ate and effects
Penta aquaticf ate and effects
 
321 samocha 9 00- final_go 100 m3
321 samocha 9 00- final_go 100 m3321 samocha 9 00- final_go 100 m3
321 samocha 9 00- final_go 100 m3
 
Use of stable and radio isotopes to understand the plant physiological process
Use of stable and radio isotopes to understand the plant physiological processUse of stable and radio isotopes to understand the plant physiological process
Use of stable and radio isotopes to understand the plant physiological process
 
OCCURRENCE, SEVERITY ASSESSMENT AND MANAGEMENT OF CITRUS GREENING IN KAVREPAL...
OCCURRENCE, SEVERITY ASSESSMENT AND MANAGEMENT OF CITRUS GREENING IN KAVREPAL...OCCURRENCE, SEVERITY ASSESSMENT AND MANAGEMENT OF CITRUS GREENING IN KAVREPAL...
OCCURRENCE, SEVERITY ASSESSMENT AND MANAGEMENT OF CITRUS GREENING IN KAVREPAL...
 
Microbiological quality of drinking water
Microbiological quality of drinking water Microbiological quality of drinking water
Microbiological quality of drinking water
 
Lab #11 – EcotoxicologyPrelab DiscussionT.docx
Lab #11 – EcotoxicologyPrelab DiscussionT.docxLab #11 – EcotoxicologyPrelab DiscussionT.docx
Lab #11 – EcotoxicologyPrelab DiscussionT.docx
 
Accumualtion of TBT and Intersex Occurence on Marine Snails Littoraria Scabra
Accumualtion of TBT and Intersex Occurence on Marine Snails Littoraria ScabraAccumualtion of TBT and Intersex Occurence on Marine Snails Littoraria Scabra
Accumualtion of TBT and Intersex Occurence on Marine Snails Littoraria Scabra
 
Accumualtion of TBT and Intersex Occurence on Marine Snails Littoraria Scabra
Accumualtion of TBT and Intersex Occurence on Marine Snails Littoraria ScabraAccumualtion of TBT and Intersex Occurence on Marine Snails Littoraria Scabra
Accumualtion of TBT and Intersex Occurence on Marine Snails Littoraria Scabra
 
Accumualtion of TBT and Intersex Occurence on Marine Snails Littoraria Scabra
Accumualtion of TBT and Intersex Occurence on Marine Snails Littoraria ScabraAccumualtion of TBT and Intersex Occurence on Marine Snails Littoraria Scabra
Accumualtion of TBT and Intersex Occurence on Marine Snails Littoraria Scabra
 
tesi finale Giulio Scalzotto
tesi finale Giulio Scalzottotesi finale Giulio Scalzotto
tesi finale Giulio Scalzotto
 
Intro Getting Your Feet Wet: Intro to Different Types of Monitoring
Intro Getting Your Feet Wet: Intro to Different Types of MonitoringIntro Getting Your Feet Wet: Intro to Different Types of Monitoring
Intro Getting Your Feet Wet: Intro to Different Types of Monitoring
 
Selection of Biological Methods for Industrial Effluents Assessments
Selection of Biological Methods for Industrial Effluents AssessmentsSelection of Biological Methods for Industrial Effluents Assessments
Selection of Biological Methods for Industrial Effluents Assessments
 
NJ AWWA Annual Conference
NJ AWWA Annual ConferenceNJ AWWA Annual Conference
NJ AWWA Annual Conference
 
Monitoring for Harmful Algal Blooms | From Data to Decisions
Monitoring for Harmful Algal Blooms | From Data to DecisionsMonitoring for Harmful Algal Blooms | From Data to Decisions
Monitoring for Harmful Algal Blooms | From Data to Decisions
 
WV-AS poster 2016
WV-AS poster 2016WV-AS poster 2016
WV-AS poster 2016
 
Minorisa minuta and the transition to secondary plastid endosymbiosis. A sing...
Minorisa minuta and the transition to secondary plastid endosymbiosis. A sing...Minorisa minuta and the transition to secondary plastid endosymbiosis. A sing...
Minorisa minuta and the transition to secondary plastid endosymbiosis. A sing...
 

Mehr von South Santee Aquaculture (10)

Macro Zyme Beneficial Bacteria Msds
Macro Zyme Beneficial Bacteria MsdsMacro Zyme Beneficial Bacteria Msds
Macro Zyme Beneficial Bacteria Msds
 
Silvercup Fish Food Manual
Silvercup Fish Food ManualSilvercup Fish Food Manual
Silvercup Fish Food Manual
 
Dnr.Sc.Gov News Yr2005 Nov1405 Nov14 Aqua.Html
Dnr.Sc.Gov News Yr2005 Nov1405 Nov14 Aqua.HtmlDnr.Sc.Gov News Yr2005 Nov1405 Nov14 Aqua.Html
Dnr.Sc.Gov News Yr2005 Nov1405 Nov14 Aqua.Html
 
Loch Ness Ss Pond Dye Msds
Loch Ness Ss Pond Dye MsdsLoch Ness Ss Pond Dye Msds
Loch Ness Ss Pond Dye Msds
 
Earth Tec Algicide Msds
Earth Tec Algicide MsdsEarth Tec Algicide Msds
Earth Tec Algicide Msds
 
Habitat Herbicide Msds
Habitat Herbicide MsdsHabitat Herbicide Msds
Habitat Herbicide Msds
 
Green Clean Pro Algicide Msds
Green Clean Pro Algicide MsdsGreen Clean Pro Algicide Msds
Green Clean Pro Algicide Msds
 
South Santee Aquaculture Brochure Web
South Santee Aquaculture Brochure WebSouth Santee Aquaculture Brochure Web
South Santee Aquaculture Brochure Web
 
Sonic Solutions Quick Reference Guide
Sonic Solutions Quick Reference GuideSonic Solutions Quick Reference Guide
Sonic Solutions Quick Reference Guide
 
Sustainable Seafood
Sustainable SeafoodSustainable Seafood
Sustainable Seafood
 

Kürzlich hochgeladen

Practical Research 1: Lesson 8 Writing the Thesis Statement.pptx
Practical Research 1: Lesson 8 Writing the Thesis Statement.pptxPractical Research 1: Lesson 8 Writing the Thesis Statement.pptx
Practical Research 1: Lesson 8 Writing the Thesis Statement.pptxKatherine Villaluna
 
What is the Future of QuickBooks DeskTop?
What is the Future of QuickBooks DeskTop?What is the Future of QuickBooks DeskTop?
What is the Future of QuickBooks DeskTop?TechSoup
 
Patient Counselling. Definition of patient counseling; steps involved in pati...
Patient Counselling. Definition of patient counseling; steps involved in pati...Patient Counselling. Definition of patient counseling; steps involved in pati...
Patient Counselling. Definition of patient counseling; steps involved in pati...raviapr7
 
General views of Histopathology and step
General views of Histopathology and stepGeneral views of Histopathology and step
General views of Histopathology and stepobaje godwin sunday
 
Patterns of Written Texts Across Disciplines.pptx
Patterns of Written Texts Across Disciplines.pptxPatterns of Written Texts Across Disciplines.pptx
Patterns of Written Texts Across Disciplines.pptxMYDA ANGELICA SUAN
 
P4C x ELT = P4ELT: Its Theoretical Background (Kanazawa, 2024 March).pdf
P4C x ELT = P4ELT: Its Theoretical Background (Kanazawa, 2024 March).pdfP4C x ELT = P4ELT: Its Theoretical Background (Kanazawa, 2024 March).pdf
P4C x ELT = P4ELT: Its Theoretical Background (Kanazawa, 2024 March).pdfYu Kanazawa / Osaka University
 
Benefits & Challenges of Inclusive Education
Benefits & Challenges of Inclusive EducationBenefits & Challenges of Inclusive Education
Benefits & Challenges of Inclusive EducationMJDuyan
 
How to Manage Cross-Selling in Odoo 17 Sales
How to Manage Cross-Selling in Odoo 17 SalesHow to Manage Cross-Selling in Odoo 17 Sales
How to Manage Cross-Selling in Odoo 17 SalesCeline George
 
Clinical Pharmacy Introduction to Clinical Pharmacy, Concept of clinical pptx
Clinical Pharmacy  Introduction to Clinical Pharmacy, Concept of clinical pptxClinical Pharmacy  Introduction to Clinical Pharmacy, Concept of clinical pptx
Clinical Pharmacy Introduction to Clinical Pharmacy, Concept of clinical pptxraviapr7
 
CapTechU Doctoral Presentation -March 2024 slides.pptx
CapTechU Doctoral Presentation -March 2024 slides.pptxCapTechU Doctoral Presentation -March 2024 slides.pptx
CapTechU Doctoral Presentation -March 2024 slides.pptxCapitolTechU
 
DUST OF SNOW_BY ROBERT FROST_EDITED BY_ TANMOY MISHRA
DUST OF SNOW_BY ROBERT FROST_EDITED BY_ TANMOY MISHRADUST OF SNOW_BY ROBERT FROST_EDITED BY_ TANMOY MISHRA
DUST OF SNOW_BY ROBERT FROST_EDITED BY_ TANMOY MISHRATanmoy Mishra
 
Easter in the USA presentation by Chloe.
Easter in the USA presentation by Chloe.Easter in the USA presentation by Chloe.
Easter in the USA presentation by Chloe.EnglishCEIPdeSigeiro
 
In - Vivo and In - Vitro Correlation.pptx
In - Vivo and In - Vitro Correlation.pptxIn - Vivo and In - Vitro Correlation.pptx
In - Vivo and In - Vitro Correlation.pptxAditiChauhan701637
 
HED Office Sohayok Exam Question Solution 2023.pdf
HED Office Sohayok Exam Question Solution 2023.pdfHED Office Sohayok Exam Question Solution 2023.pdf
HED Office Sohayok Exam Question Solution 2023.pdfMohonDas
 
Practical Research 1 Lesson 9 Scope and delimitation.pptx
Practical Research 1 Lesson 9 Scope and delimitation.pptxPractical Research 1 Lesson 9 Scope and delimitation.pptx
Practical Research 1 Lesson 9 Scope and delimitation.pptxKatherine Villaluna
 
Ultra structure and life cycle of Plasmodium.pptx
Ultra structure and life cycle of Plasmodium.pptxUltra structure and life cycle of Plasmodium.pptx
Ultra structure and life cycle of Plasmodium.pptxDr. Asif Anas
 
How to Show Error_Warning Messages in Odoo 17
How to Show Error_Warning Messages in Odoo 17How to Show Error_Warning Messages in Odoo 17
How to Show Error_Warning Messages in Odoo 17Celine George
 
How to Use api.constrains ( ) in Odoo 17
How to Use api.constrains ( ) in Odoo 17How to Use api.constrains ( ) in Odoo 17
How to Use api.constrains ( ) in Odoo 17Celine George
 
Education and training program in the hospital APR.pptx
Education and training program in the hospital APR.pptxEducation and training program in the hospital APR.pptx
Education and training program in the hospital APR.pptxraviapr7
 
The basics of sentences session 10pptx.pptx
The basics of sentences session 10pptx.pptxThe basics of sentences session 10pptx.pptx
The basics of sentences session 10pptx.pptxheathfieldcps1
 

Kürzlich hochgeladen (20)

Practical Research 1: Lesson 8 Writing the Thesis Statement.pptx
Practical Research 1: Lesson 8 Writing the Thesis Statement.pptxPractical Research 1: Lesson 8 Writing the Thesis Statement.pptx
Practical Research 1: Lesson 8 Writing the Thesis Statement.pptx
 
What is the Future of QuickBooks DeskTop?
What is the Future of QuickBooks DeskTop?What is the Future of QuickBooks DeskTop?
What is the Future of QuickBooks DeskTop?
 
Patient Counselling. Definition of patient counseling; steps involved in pati...
Patient Counselling. Definition of patient counseling; steps involved in pati...Patient Counselling. Definition of patient counseling; steps involved in pati...
Patient Counselling. Definition of patient counseling; steps involved in pati...
 
General views of Histopathology and step
General views of Histopathology and stepGeneral views of Histopathology and step
General views of Histopathology and step
 
Patterns of Written Texts Across Disciplines.pptx
Patterns of Written Texts Across Disciplines.pptxPatterns of Written Texts Across Disciplines.pptx
Patterns of Written Texts Across Disciplines.pptx
 
P4C x ELT = P4ELT: Its Theoretical Background (Kanazawa, 2024 March).pdf
P4C x ELT = P4ELT: Its Theoretical Background (Kanazawa, 2024 March).pdfP4C x ELT = P4ELT: Its Theoretical Background (Kanazawa, 2024 March).pdf
P4C x ELT = P4ELT: Its Theoretical Background (Kanazawa, 2024 March).pdf
 
Benefits & Challenges of Inclusive Education
Benefits & Challenges of Inclusive EducationBenefits & Challenges of Inclusive Education
Benefits & Challenges of Inclusive Education
 
How to Manage Cross-Selling in Odoo 17 Sales
How to Manage Cross-Selling in Odoo 17 SalesHow to Manage Cross-Selling in Odoo 17 Sales
How to Manage Cross-Selling in Odoo 17 Sales
 
Clinical Pharmacy Introduction to Clinical Pharmacy, Concept of clinical pptx
Clinical Pharmacy  Introduction to Clinical Pharmacy, Concept of clinical pptxClinical Pharmacy  Introduction to Clinical Pharmacy, Concept of clinical pptx
Clinical Pharmacy Introduction to Clinical Pharmacy, Concept of clinical pptx
 
CapTechU Doctoral Presentation -March 2024 slides.pptx
CapTechU Doctoral Presentation -March 2024 slides.pptxCapTechU Doctoral Presentation -March 2024 slides.pptx
CapTechU Doctoral Presentation -March 2024 slides.pptx
 
DUST OF SNOW_BY ROBERT FROST_EDITED BY_ TANMOY MISHRA
DUST OF SNOW_BY ROBERT FROST_EDITED BY_ TANMOY MISHRADUST OF SNOW_BY ROBERT FROST_EDITED BY_ TANMOY MISHRA
DUST OF SNOW_BY ROBERT FROST_EDITED BY_ TANMOY MISHRA
 
Easter in the USA presentation by Chloe.
Easter in the USA presentation by Chloe.Easter in the USA presentation by Chloe.
Easter in the USA presentation by Chloe.
 
In - Vivo and In - Vitro Correlation.pptx
In - Vivo and In - Vitro Correlation.pptxIn - Vivo and In - Vitro Correlation.pptx
In - Vivo and In - Vitro Correlation.pptx
 
HED Office Sohayok Exam Question Solution 2023.pdf
HED Office Sohayok Exam Question Solution 2023.pdfHED Office Sohayok Exam Question Solution 2023.pdf
HED Office Sohayok Exam Question Solution 2023.pdf
 
Practical Research 1 Lesson 9 Scope and delimitation.pptx
Practical Research 1 Lesson 9 Scope and delimitation.pptxPractical Research 1 Lesson 9 Scope and delimitation.pptx
Practical Research 1 Lesson 9 Scope and delimitation.pptx
 
Ultra structure and life cycle of Plasmodium.pptx
Ultra structure and life cycle of Plasmodium.pptxUltra structure and life cycle of Plasmodium.pptx
Ultra structure and life cycle of Plasmodium.pptx
 
How to Show Error_Warning Messages in Odoo 17
How to Show Error_Warning Messages in Odoo 17How to Show Error_Warning Messages in Odoo 17
How to Show Error_Warning Messages in Odoo 17
 
How to Use api.constrains ( ) in Odoo 17
How to Use api.constrains ( ) in Odoo 17How to Use api.constrains ( ) in Odoo 17
How to Use api.constrains ( ) in Odoo 17
 
Education and training program in the hospital APR.pptx
Education and training program in the hospital APR.pptxEducation and training program in the hospital APR.pptx
Education and training program in the hospital APR.pptx
 
The basics of sentences session 10pptx.pptx
The basics of sentences session 10pptx.pptxThe basics of sentences session 10pptx.pptx
The basics of sentences session 10pptx.pptx
 

Golden Algae

  • 1. Prymnesium parvum : Analysis of Pigmentation Dynamics Over Growth by Alexis Martinez Identification through Bio-Optical Signatures by Merissa Ludwig REU Program Texas A&M University Summer 2005
  • 2.
  • 3.
  • 4. Golden Algae Family Tree Illustrations by Robert G. Howells, TPWD
  • 5.
  • 6.
  • 7.
  • 8.
  • 9.
  • 10.
  • 11.  
  • 12.
  • 13.  
  • 14.
  • 15.
  • 16. Problem Research Solution Persistent toxic algae with the ability to decimate fish, bivalve, and other phytoplankton populations. Texas economy feels effect in declining tourism and recreation due to dead fish. Knowledge about P. parvum is limited, inability to detect indicators prior to harmful algal bloom. Immediate need for simple, rapid, and more accurate method to detect P. parvum and to identify possible indicators before harmful algal bloom.
  • 17.
  • 18.
  • 19.
  • 20.
  • 21.  
  • 22.
  • 23.
  • 24.
  • 25.
  • 26. High-Performance Liquid Chromatography Fig. 1. Preparative and analytical procedures for HPLC from Millie et. al. (1993).
  • 27.
  • 28.
  • 29.
  • 31.  
  • 32.  
  • 33. A B C
  • 34. Pigment ratio statistics of entire sampling period (Day 3 – 24) 57 30 38 18 24 15 COV %   0.01 0.06 0.01 0.09 0.04 0.01 STDV   0.03 0.20 0.01 0.48 0.15 0.05 AVG C     39 24 53 21 23 27 COV %   0.01 0.05 0.01 0.11 0.04 0.02 STDV   0.03 0.23 0.01 0.53 0.18 0.06 AVG B     56 13 57 12 21 33 COV %   0.01 0.03 0.01 0.06 0.03 0.02 STDV   0.02 0.19 0.01 0.49 0.16 0.05 AVG A Zeax- Diado-/Diato Viola- Fuco- Chl c1c2 Chl c3 TOTAL  
  • 35. Growth Phase Pigmentation Dynamics (highlighted areas display very high correlation of coefficient values) 86 23 40 14 21 16 COV %   0.01 0.04 0.01 0.07 0.03 0.01 STDV   0.02 0.16 0.01 0.47 0.13 0.05 AVG C     56 28 82 25 31 39 COV %   0.01 0.06 0.01 0.14 0.05 0.02 STDV   0.02 0.21 0.01 0.55 0.16 0.06 AVG B     74 10 90 10 30 42 COV %   0.01 0.02 0.01 0.05 0.05 0.02 STDV   0.01 0.17 0.01 0.50 0.15 0.06 AVG A Zeax- Diado-/Diato Viola- Fuco- Chl c1c2 Chl c3 GROWTH  
  • 36. Stationary Phase Pigmentation Dynamics 20 0 47 12 27 0 COV %   0.01 0.00 0.01 0.06 0.04 0.00 STDV   0.04 0.23 0.02 0.48 0.16 0.05 AVG C     16 23 0 12 13 17 COV %   0.01 0.06 0.00 0.07 0.03 0.01 STDV   0.04 0.25 0.01 0.55 0.20 0.06 AVG B     29 5 11 13 7 15 COV %   0.01 0.01 0.00 0.07 0.01 0.01 STDV   0.03 0.21 0.01 0.50 0.18 0.05 AVG A Zeax- Diado-/Diato Viola- Fuco- Chl c1c2 Chl c3 STATIONARY  
  • 37. Comparison of separating analysis over phases versus using entire sampling period 57 30 38 18 24 15 TOTAL   20 0 47 12 27 0 STATIONARY   86 23 40 14 21 16 GROWTH C       39 24 53 21 23 27 TOTAL   16 23 0 12 13 17 STATIONARY   56 28 82 25 31 39 GROWTH B       56 13 57 12 21 33 TOTAL   29 5 11 13 7 15 STATIONARY   74 10 90 10 30 42 GROWTH A Zeax- Diado-/Diato Viola- Fuco- Chl c1c2 Chl c3 COV %  
  • 38.
  • 39. Rapid Detection through use of Optical Signatures Merissa Ludwig
  • 40.
  • 41.
  • 42.
  • 43.
  • 44.
  • 46.
  • 47.
  • 48.
  • 49.
  • 50.  
  • 51.