SlideShare a Scribd company logo
1 of 10
Carotid Atherosclerotic Lesion Models
for Quality Control
of Clinical Trials
with MRI Endpoints
William Insull, M.D., Gareth Adams, B.S.
Cassius Bordelon, Ph.D. and Joel Morrisett, Ph.D.
Baylor College of Medicine
Houston,Texas
The Model Attributes
should closely resemble those of human carotid
arteries in terms of:
a) gross and microscopic structure and dimensions
b) spatial distribution of principal components
c) chemical composition of principal components
d) presence of normal and atherosclerotic tissues
e) presence of all stages of atherosclerotic lesions
f) identifiable zones of transition from normal to
atherosclerotic regions for indexing lesion
initiation, development, and growth
Model Attributes (continued)
g) identifiable major lesion types for indexing lesion changes and the
effects of treatment (retardation, stabilization, regression)
h) defined sites for replicate measures, whose locations are referenced to
the carotid bifurcation or an external marker
i) gross dimensions, histology, and chemical composition at reference
sites as defined by accepted independent measurements
(e.g. X-ray, raman, infrared spectroscopies)
j) multiple reference sites indexed for easy use
k) stability for extended time periods (e.g. years) in chemical
composition, and gross and microscopic morphology
The Model Holder
should:
a) have clear identification permanently attached
b) allow easy access to indexed sites
c) provide mounting that makes the model resistant to normal wear
d) be compatible with conventional and state-of-the-art MRI
instrumentation
e) be amenable to convenient handling and shipping
f) have attached instructions for handling by the user
Model Documentation
should provide:
a) accurate description of the gross and histological
composition, with location of defined, indexed referenced sites
for rapid orientation in scanning and measurement
b) an atlas of acceptable images
c) specifications for MR image acquisition at indexed
reference sites
d) defined quality control parameters with the accepted “in
control” range
e) periodic verification of stability of MRI characteristics
performed by the reference laboratory
f) life history of the model
CCA
BIF
ICA
ECA
Cadaveric human carotid artery used for Reference Standard
Tissue was perfusion fixed at mean arterial pressure before excision.
Most of the periadventitial tissue has been removed. The common
carotid artery (CCA), bifurcation (BIF), external carotid artery (ECA),
and internal carotid artery (ICA) are clearly seen.
Cadaveric Carotid Specimens
( models of the native carotid arteries)
-contain intima, media, adventitia, and
some perivascular soft tissue
-enable study of range of lesion types
-allow comparison of left/right carotids
-give reproducible images over >1 year
-provide stable reference for:
-intra-laboratory standardization
-inter-laboratory standardization
for multicenter clinical trials
Holder for imaging cadaveric carotid tissues.
The tubes (3.0cm diameter) are secured in four holes drilled in a plastic insert (8.0cm diameter)
fabricated from two 100ml plastic beakers fastened top-to-top by superglue. The insert is
placed in a larger plastic bottle (12.0cm diameter, 22.0cm tall) fitted with a water inlet at the
bottom and outlet at the top for temperature control. A pair of phased array coils (6cm Ultra
Image/Pathway) is fastened around the outside of the larger outer bottle with velcro strips.
Tissues are imaged in a GE Horizon 1.5T clinical MRI system with 10cm FOV, 2 NEX,
3mm slice thickness. TR/TE for the different contrast sequences: 3000/34 (PDW), 650/14
(T1W), and 3000/108 (T2W).
1.5T MR images of left and right human cadaveric carotid arteries
above, at, and below their bifurcation.
The plaque within each tissue slice (red area) has been identified by a
semi-automatic algorithm. 3D reconstructions from multiple slices are
used to calculate the aggregate volumes.
PDW
T1W
T2W
MR images of human cadaveric carotids acquired on a GE Horizon
LX 1.5T system fitted with UltraImage/Pathway phased array coils (also
used for human carotid studies in vivo). The slices (3mm) begin
at the bifurcation (left) and descend to the common carotid artery (right).
Proton density-, T1-, and T2- weighted images show plaque heterogeneity

More Related Content

What's hot

Published Paper 2011
Published Paper 2011Published Paper 2011
Published Paper 2011
Bassel Samman
 
Koutsiaris 2013_b_ΜRI BLADE_Lumbar Spine
Koutsiaris 2013_b_ΜRI BLADE_Lumbar SpineKoutsiaris 2013_b_ΜRI BLADE_Lumbar Spine
Koutsiaris 2013_b_ΜRI BLADE_Lumbar Spine
Koutsiaris Aris
 

What's hot (11)

Optical coherence tomography jang-mgh
Optical coherence tomography jang-mghOptical coherence tomography jang-mgh
Optical coherence tomography jang-mgh
 
Oct with shourt phontonic needle
Oct with shourt phontonic needleOct with shourt phontonic needle
Oct with shourt phontonic needle
 
Aeha aha 2002 poster
Aeha aha 2002 posterAeha aha 2002 poster
Aeha aha 2002 poster
 
130 mri assessment of human atherosclerotic disease
130 mri assessment of human atherosclerotic disease130 mri assessment of human atherosclerotic disease
130 mri assessment of human atherosclerotic disease
 
Intensity Non-uniformity Correction for Image Segmentation
Intensity Non-uniformity Correction for Image SegmentationIntensity Non-uniformity Correction for Image Segmentation
Intensity Non-uniformity Correction for Image Segmentation
 
The Acoustic Technology for Ctcs Isolation in Blood: Low-Cost Devices_Crimson...
The Acoustic Technology for Ctcs Isolation in Blood: Low-Cost Devices_Crimson...The Acoustic Technology for Ctcs Isolation in Blood: Low-Cost Devices_Crimson...
The Acoustic Technology for Ctcs Isolation in Blood: Low-Cost Devices_Crimson...
 
New_IHBI_POSTER. last version.ppt
New_IHBI_POSTER. last version.pptNew_IHBI_POSTER. last version.ppt
New_IHBI_POSTER. last version.ppt
 
Published Paper 2011
Published Paper 2011Published Paper 2011
Published Paper 2011
 
Koutsiaris 2013_b_ΜRI BLADE_Lumbar Spine
Koutsiaris 2013_b_ΜRI BLADE_Lumbar SpineKoutsiaris 2013_b_ΜRI BLADE_Lumbar Spine
Koutsiaris 2013_b_ΜRI BLADE_Lumbar Spine
 
Example
ExampleExample
Example
 
SoA presentation
SoA presentationSoA presentation
SoA presentation
 

Viewers also liked

065 association for eradication of heart attack
065 association for eradication of heart attack065 association for eradication of heart attack
065 association for eradication of heart attack
SHAPE Society
 
235 does cd40 link atherosclerosis to thrombosis
235 does cd40 link atherosclerosis to thrombosis235 does cd40 link atherosclerosis to thrombosis
235 does cd40 link atherosclerosis to thrombosis
SHAPE Society
 
167 plaque p h heterogeneity in physiological media
167 plaque p h heterogeneity in physiological media167 plaque p h heterogeneity in physiological media
167 plaque p h heterogeneity in physiological media
SHAPE Society
 
130 mri assessment of human atherosclerotic disease
130 mri assessment of human atherosclerotic disease130 mri assessment of human atherosclerotic disease
130 mri assessment of human atherosclerotic disease
SHAPE Society
 
053 histologic evidence of spio accumulation in vulnerable plaques
053 histologic evidence of spio accumulation in vulnerable plaques053 histologic evidence of spio accumulation in vulnerable plaques
053 histologic evidence of spio accumulation in vulnerable plaques
SHAPE Society
 
212 basic molecular genetic studies in atherosclerosis
212 basic molecular genetic studies in atherosclerosis212 basic molecular genetic studies in atherosclerosis
212 basic molecular genetic studies in atherosclerosis
SHAPE Society
 

Viewers also liked (20)

222 animal models of heart attack
222 animal models of heart attack222 animal models of heart attack
222 animal models of heart attack
 
3rd vulnerable plaque rumberger 3 16-02 4
3rd vulnerable plaque rumberger 3 16-02 43rd vulnerable plaque rumberger 3 16-02 4
3rd vulnerable plaque rumberger 3 16-02 4
 
261 molecular genetics and micro arrays
261 molecular genetics and micro arrays261 molecular genetics and micro arrays
261 molecular genetics and micro arrays
 
162 calcium imaging
162 calcium imaging162 calcium imaging
162 calcium imaging
 
152 spio photographs
152 spio photographs152 spio photographs
152 spio photographs
 
065 association for eradication of heart attack
065 association for eradication of heart attack065 association for eradication of heart attack
065 association for eradication of heart attack
 
235 does cd40 link atherosclerosis to thrombosis
235 does cd40 link atherosclerosis to thrombosis235 does cd40 link atherosclerosis to thrombosis
235 does cd40 link atherosclerosis to thrombosis
 
167 plaque p h heterogeneity in physiological media
167 plaque p h heterogeneity in physiological media167 plaque p h heterogeneity in physiological media
167 plaque p h heterogeneity in physiological media
 
130 mri assessment of human atherosclerotic disease
130 mri assessment of human atherosclerotic disease130 mri assessment of human atherosclerotic disease
130 mri assessment of human atherosclerotic disease
 
053 histologic evidence of spio accumulation in vulnerable plaques
053 histologic evidence of spio accumulation in vulnerable plaques053 histologic evidence of spio accumulation in vulnerable plaques
053 histologic evidence of spio accumulation in vulnerable plaques
 
212 basic molecular genetic studies in atherosclerosis
212 basic molecular genetic studies in atherosclerosis212 basic molecular genetic studies in atherosclerosis
212 basic molecular genetic studies in atherosclerosis
 
5
55
5
 
128 apo e deficient mice
128 apo e deficient mice128 apo e deficient mice
128 apo e deficient mice
 
103100
103100103100
103100
 
118 cell culture study
118 cell culture study118 cell culture study
118 cell culture study
 
239 coronary artery calcium
239 coronary artery calcium239 coronary artery calcium
239 coronary artery calcium
 
257 inhibition of atherosclerosis in mice
257 inhibition of atherosclerosis in mice257 inhibition of atherosclerosis in mice
257 inhibition of atherosclerosis in mice
 
3rd vulnerable plaque rumberg 3 16-02 2
3rd vulnerable plaque rumberg 3 16-02 23rd vulnerable plaque rumberg 3 16-02 2
3rd vulnerable plaque rumberg 3 16-02 2
 
236 complement inhibitor
236 complement inhibitor236 complement inhibitor
236 complement inhibitor
 
151 performance of a localized fiber optic
151 performance of a localized fiber optic151 performance of a localized fiber optic
151 performance of a localized fiber optic
 

Similar to 248 carotid atherosclerotic lesion models

Atlas of advanced endoaortic surgery
Atlas of advanced endoaortic surgeryAtlas of advanced endoaortic surgery
Atlas of advanced endoaortic surgery
Springer
 
Atlas of advanced endoaortic surgery
Atlas of advanced endoaortic surgeryAtlas of advanced endoaortic surgery
Atlas of advanced endoaortic surgery
Springer
 
Hofer ct teaching manual - a systematic approach to ct reading, 2nd ed.
Hofer   ct teaching manual - a systematic approach to ct reading,  2nd ed.Hofer   ct teaching manual - a systematic approach to ct reading,  2nd ed.
Hofer ct teaching manual - a systematic approach to ct reading, 2nd ed.
Michel Phuong
 
HDMICS WSS Koutsiaris 2007
HDMICS WSS Koutsiaris 2007HDMICS WSS Koutsiaris 2007
HDMICS WSS Koutsiaris 2007
Koutsiaris Aris
 
Site-specific intravascular ultrasound analysis of remodelling index and calc...
Site-specific intravascular ultrasound analysis of remodelling index and calc...Site-specific intravascular ultrasound analysis of remodelling index and calc...
Site-specific intravascular ultrasound analysis of remodelling index and calc...
Cardiovascular Diagnosis and Therapy (CDT)
 
Measuring of migration of a fully automated imaging based approach
Measuring of migration of a fully automated imaging based approachMeasuring of migration of a fully automated imaging based approach
Measuring of migration of a fully automated imaging based approach
PerkinElmer, Inc.
 
Dose delivered from varian's cbct to patients receiving imrt for prostate cancer
Dose delivered from varian's cbct to patients receiving imrt for prostate cancerDose delivered from varian's cbct to patients receiving imrt for prostate cancer
Dose delivered from varian's cbct to patients receiving imrt for prostate cancer
lanying
 
Feasibility of using 3D MR elastography to determine pancreatic stiffness in ...
Feasibility of using 3D MR elastography to determine pancreatic stiffness in ...Feasibility of using 3D MR elastography to determine pancreatic stiffness in ...
Feasibility of using 3D MR elastography to determine pancreatic stiffness in ...
Nicole Freitag
 

Similar to 248 carotid atherosclerotic lesion models (20)

HDMICS Koutsiaris 2010d
HDMICS Koutsiaris 2010dHDMICS Koutsiaris 2010d
HDMICS Koutsiaris 2010d
 
Atlas of advanced endoaortic surgery
Atlas of advanced endoaortic surgeryAtlas of advanced endoaortic surgery
Atlas of advanced endoaortic surgery
 
Atlas of advanced endoaortic surgery
Atlas of advanced endoaortic surgeryAtlas of advanced endoaortic surgery
Atlas of advanced endoaortic surgery
 
Koutsiaris 2013_a
Koutsiaris 2013_aKoutsiaris 2013_a
Koutsiaris 2013_a
 
Hofer ct teaching manual - a systematic approach to ct reading, 2nd ed.
Hofer   ct teaching manual - a systematic approach to ct reading,  2nd ed.Hofer   ct teaching manual - a systematic approach to ct reading,  2nd ed.
Hofer ct teaching manual - a systematic approach to ct reading, 2nd ed.
 
CT Angiography Lower Limb
CT Angiography Lower LimbCT Angiography Lower Limb
CT Angiography Lower Limb
 
Art19
Art19Art19
Art19
 
HDMICS WSS Koutsiaris 2007
HDMICS WSS Koutsiaris 2007HDMICS WSS Koutsiaris 2007
HDMICS WSS Koutsiaris 2007
 
174 multicontrast mri
174 multicontrast mri174 multicontrast mri
174 multicontrast mri
 
Applying Deep Learning Techniques in Automated Analysis of CT scan images for...
Applying Deep Learning Techniques in Automated Analysis of CT scan images for...Applying Deep Learning Techniques in Automated Analysis of CT scan images for...
Applying Deep Learning Techniques in Automated Analysis of CT scan images for...
 
Site-specific intravascular ultrasound analysis of remodelling index and calc...
Site-specific intravascular ultrasound analysis of remodelling index and calc...Site-specific intravascular ultrasound analysis of remodelling index and calc...
Site-specific intravascular ultrasound analysis of remodelling index and calc...
 
Measuring of migration of a fully automated imaging based approach
Measuring of migration of a fully automated imaging based approachMeasuring of migration of a fully automated imaging based approach
Measuring of migration of a fully automated imaging based approach
 
AUTOMATED SEGMENTATION OF FLUORESCENT AND FUNDS IMAGES BASED ON RETINAL BLOOD...
AUTOMATED SEGMENTATION OF FLUORESCENT AND FUNDS IMAGES BASED ON RETINAL BLOOD...AUTOMATED SEGMENTATION OF FLUORESCENT AND FUNDS IMAGES BASED ON RETINAL BLOOD...
AUTOMATED SEGMENTATION OF FLUORESCENT AND FUNDS IMAGES BASED ON RETINAL BLOOD...
 
AUTOMATED SEGMENTATION OF FLUORESCENT AND FUNDS IMAGES BASED ON RETINAL BLOOD...
AUTOMATED SEGMENTATION OF FLUORESCENT AND FUNDS IMAGES BASED ON RETINAL BLOOD...AUTOMATED SEGMENTATION OF FLUORESCENT AND FUNDS IMAGES BASED ON RETINAL BLOOD...
AUTOMATED SEGMENTATION OF FLUORESCENT AND FUNDS IMAGES BASED ON RETINAL BLOOD...
 
Dose delivered from varian's cbct to patients receiving imrt for prostate cancer
Dose delivered from varian's cbct to patients receiving imrt for prostate cancerDose delivered from varian's cbct to patients receiving imrt for prostate cancer
Dose delivered from varian's cbct to patients receiving imrt for prostate cancer
 
Ann Vasc Surg 2012 Funnel Technique
Ann Vasc Surg 2012 Funnel TechniqueAnn Vasc Surg 2012 Funnel Technique
Ann Vasc Surg 2012 Funnel Technique
 
MR Elastography
MR ElastographyMR Elastography
MR Elastography
 
3 d crt
3 d crt3 d crt
3 d crt
 
Feasibility of using 3D MR elastography to determine pancreatic stiffness in ...
Feasibility of using 3D MR elastography to determine pancreatic stiffness in ...Feasibility of using 3D MR elastography to determine pancreatic stiffness in ...
Feasibility of using 3D MR elastography to determine pancreatic stiffness in ...
 
CTO and bifurcation
CTO and bifurcationCTO and bifurcation
CTO and bifurcation
 

More from SHAPE Society (20)

Barth imt
Barth imtBarth imt
Barth imt
 
Braunwald
BraunwaldBraunwald
Braunwald
 
Acc vp-org 3-02 stone
Acc vp-org 3-02 stoneAcc vp-org 3-02 stone
Acc vp-org 3-02 stone
 
101205 shape aha 2005 f
101205 shape aha 2005 f101205 shape aha 2005 f
101205 shape aha 2005 f
 
Acc v porg becker
Acc v porg beckerAcc v porg becker
Acc v porg becker
 
Acc 2005-1, v pl-vp
Acc 2005-1, v pl-vpAcc 2005-1, v pl-vp
Acc 2005-1, v pl-vp
 
Acc presentation macrophage (1)
Acc presentation macrophage (1)Acc presentation macrophage (1)
Acc presentation macrophage (1)
 
Acc v porg
Acc v porgAcc v porg
Acc v porg
 
Acc presentation (1)
Acc presentation (1)Acc presentation (1)
Acc presentation (1)
 
Ac cculprit
Ac cculpritAc cculprit
Ac cculprit
 
Acc presentation lipid coated(2)
Acc presentation lipid coated(2)Acc presentation lipid coated(2)
Acc presentation lipid coated(2)
 
Acc 2002 microarray mehran for print
Acc 2002 microarray mehran for printAcc 2002 microarray mehran for print
Acc 2002 microarray mehran for print
 
Acc booth inventory
Acc booth inventoryAcc booth inventory
Acc booth inventory
 
Acc 04 vuln pt
Acc 04 vuln ptAcc 04 vuln pt
Acc 04 vuln pt
 
Acc 2002 mehran for print
Acc 2002  mehran for printAcc 2002  mehran for print
Acc 2002 mehran for print
 
Acc2002 thermography
Acc2002 thermographyAcc2002 thermography
Acc2002 thermography
 
A20 gene
A20 geneA20 gene
A20 gene
 
#1 killer of human beings in the 21st century
#1 killer of human beings in the 21st century#1 killer of human beings in the 21st century
#1 killer of human beings in the 21st century
 
A20 is an atherosclerosis
A20 is an atherosclerosisA20 is an atherosclerosis
A20 is an atherosclerosis
 
259 crp as a risk factor
259 crp as a risk factor259 crp as a risk factor
259 crp as a risk factor
 

Recently uploaded

Call Girls Aurangabad Just Call 8250077686 Top Class Call Girl Service Available
Call Girls Aurangabad Just Call 8250077686 Top Class Call Girl Service AvailableCall Girls Aurangabad Just Call 8250077686 Top Class Call Girl Service Available
Call Girls Aurangabad Just Call 8250077686 Top Class Call Girl Service Available
Dipal Arora
 

Recently uploaded (20)

Call Girls Nagpur Just Call 9907093804 Top Class Call Girl Service Available
Call Girls Nagpur Just Call 9907093804 Top Class Call Girl Service AvailableCall Girls Nagpur Just Call 9907093804 Top Class Call Girl Service Available
Call Girls Nagpur Just Call 9907093804 Top Class Call Girl Service Available
 
Bangalore Call Girls Nelamangala Number 9332606886 Meetin With Bangalore Esc...
Bangalore Call Girls Nelamangala Number 9332606886  Meetin With Bangalore Esc...Bangalore Call Girls Nelamangala Number 9332606886  Meetin With Bangalore Esc...
Bangalore Call Girls Nelamangala Number 9332606886 Meetin With Bangalore Esc...
 
Best Rate (Patna ) Call Girls Patna ⟟ 8617370543 ⟟ High Class Call Girl In 5 ...
Best Rate (Patna ) Call Girls Patna ⟟ 8617370543 ⟟ High Class Call Girl In 5 ...Best Rate (Patna ) Call Girls Patna ⟟ 8617370543 ⟟ High Class Call Girl In 5 ...
Best Rate (Patna ) Call Girls Patna ⟟ 8617370543 ⟟ High Class Call Girl In 5 ...
 
Call Girls Faridabad Just Call 9907093804 Top Class Call Girl Service Available
Call Girls Faridabad Just Call 9907093804 Top Class Call Girl Service AvailableCall Girls Faridabad Just Call 9907093804 Top Class Call Girl Service Available
Call Girls Faridabad Just Call 9907093804 Top Class Call Girl Service Available
 
Call Girls Horamavu WhatsApp Number 7001035870 Meeting With Bangalore Escorts
Call Girls Horamavu WhatsApp Number 7001035870 Meeting With Bangalore EscortsCall Girls Horamavu WhatsApp Number 7001035870 Meeting With Bangalore Escorts
Call Girls Horamavu WhatsApp Number 7001035870 Meeting With Bangalore Escorts
 
♛VVIP Hyderabad Call Girls Chintalkunta🖕7001035870🖕Riya Kappor Top Call Girl ...
♛VVIP Hyderabad Call Girls Chintalkunta🖕7001035870🖕Riya Kappor Top Call Girl ...♛VVIP Hyderabad Call Girls Chintalkunta🖕7001035870🖕Riya Kappor Top Call Girl ...
♛VVIP Hyderabad Call Girls Chintalkunta🖕7001035870🖕Riya Kappor Top Call Girl ...
 
Night 7k to 12k Navi Mumbai Call Girl Photo 👉 BOOK NOW 9833363713 👈 ♀️ night ...
Night 7k to 12k Navi Mumbai Call Girl Photo 👉 BOOK NOW 9833363713 👈 ♀️ night ...Night 7k to 12k Navi Mumbai Call Girl Photo 👉 BOOK NOW 9833363713 👈 ♀️ night ...
Night 7k to 12k Navi Mumbai Call Girl Photo 👉 BOOK NOW 9833363713 👈 ♀️ night ...
 
Call Girls Ludhiana Just Call 9907093804 Top Class Call Girl Service Available
Call Girls Ludhiana Just Call 9907093804 Top Class Call Girl Service AvailableCall Girls Ludhiana Just Call 9907093804 Top Class Call Girl Service Available
Call Girls Ludhiana Just Call 9907093804 Top Class Call Girl Service Available
 
Manyata Tech Park ( Call Girls ) Bangalore ✔ 6297143586 ✔ Hot Model With Sexy...
Manyata Tech Park ( Call Girls ) Bangalore ✔ 6297143586 ✔ Hot Model With Sexy...Manyata Tech Park ( Call Girls ) Bangalore ✔ 6297143586 ✔ Hot Model With Sexy...
Manyata Tech Park ( Call Girls ) Bangalore ✔ 6297143586 ✔ Hot Model With Sexy...
 
Lucknow Call girls - 8800925952 - 24x7 service with hotel room
Lucknow Call girls - 8800925952 - 24x7 service with hotel roomLucknow Call girls - 8800925952 - 24x7 service with hotel room
Lucknow Call girls - 8800925952 - 24x7 service with hotel room
 
Call Girls Coimbatore Just Call 9907093804 Top Class Call Girl Service Available
Call Girls Coimbatore Just Call 9907093804 Top Class Call Girl Service AvailableCall Girls Coimbatore Just Call 9907093804 Top Class Call Girl Service Available
Call Girls Coimbatore Just Call 9907093804 Top Class Call Girl Service Available
 
Call Girls Visakhapatnam Just Call 9907093804 Top Class Call Girl Service Ava...
Call Girls Visakhapatnam Just Call 9907093804 Top Class Call Girl Service Ava...Call Girls Visakhapatnam Just Call 9907093804 Top Class Call Girl Service Ava...
Call Girls Visakhapatnam Just Call 9907093804 Top Class Call Girl Service Ava...
 
Premium Call Girls Cottonpet Whatsapp 7001035870 Independent Escort Service
Premium Call Girls Cottonpet Whatsapp 7001035870 Independent Escort ServicePremium Call Girls Cottonpet Whatsapp 7001035870 Independent Escort Service
Premium Call Girls Cottonpet Whatsapp 7001035870 Independent Escort Service
 
💎VVIP Kolkata Call Girls Parganas🩱7001035870🩱Independent Girl ( Ac Rooms Avai...
💎VVIP Kolkata Call Girls Parganas🩱7001035870🩱Independent Girl ( Ac Rooms Avai...💎VVIP Kolkata Call Girls Parganas🩱7001035870🩱Independent Girl ( Ac Rooms Avai...
💎VVIP Kolkata Call Girls Parganas🩱7001035870🩱Independent Girl ( Ac Rooms Avai...
 
Call Girls Cuttack Just Call 9907093804 Top Class Call Girl Service Available
Call Girls Cuttack Just Call 9907093804 Top Class Call Girl Service AvailableCall Girls Cuttack Just Call 9907093804 Top Class Call Girl Service Available
Call Girls Cuttack Just Call 9907093804 Top Class Call Girl Service Available
 
Call Girls Tirupati Just Call 8250077686 Top Class Call Girl Service Available
Call Girls Tirupati Just Call 8250077686 Top Class Call Girl Service AvailableCall Girls Tirupati Just Call 8250077686 Top Class Call Girl Service Available
Call Girls Tirupati Just Call 8250077686 Top Class Call Girl Service Available
 
(Rocky) Jaipur Call Girl - 09521753030 Escorts Service 50% Off with Cash ON D...
(Rocky) Jaipur Call Girl - 09521753030 Escorts Service 50% Off with Cash ON D...(Rocky) Jaipur Call Girl - 09521753030 Escorts Service 50% Off with Cash ON D...
(Rocky) Jaipur Call Girl - 09521753030 Escorts Service 50% Off with Cash ON D...
 
Best Rate (Hyderabad) Call Girls Jahanuma ⟟ 8250192130 ⟟ High Class Call Girl...
Best Rate (Hyderabad) Call Girls Jahanuma ⟟ 8250192130 ⟟ High Class Call Girl...Best Rate (Hyderabad) Call Girls Jahanuma ⟟ 8250192130 ⟟ High Class Call Girl...
Best Rate (Hyderabad) Call Girls Jahanuma ⟟ 8250192130 ⟟ High Class Call Girl...
 
Call Girls Bareilly Just Call 8250077686 Top Class Call Girl Service Available
Call Girls Bareilly Just Call 8250077686 Top Class Call Girl Service AvailableCall Girls Bareilly Just Call 8250077686 Top Class Call Girl Service Available
Call Girls Bareilly Just Call 8250077686 Top Class Call Girl Service Available
 
Call Girls Aurangabad Just Call 8250077686 Top Class Call Girl Service Available
Call Girls Aurangabad Just Call 8250077686 Top Class Call Girl Service AvailableCall Girls Aurangabad Just Call 8250077686 Top Class Call Girl Service Available
Call Girls Aurangabad Just Call 8250077686 Top Class Call Girl Service Available
 

248 carotid atherosclerotic lesion models

  • 1. Carotid Atherosclerotic Lesion Models for Quality Control of Clinical Trials with MRI Endpoints William Insull, M.D., Gareth Adams, B.S. Cassius Bordelon, Ph.D. and Joel Morrisett, Ph.D. Baylor College of Medicine Houston,Texas
  • 2. The Model Attributes should closely resemble those of human carotid arteries in terms of: a) gross and microscopic structure and dimensions b) spatial distribution of principal components c) chemical composition of principal components d) presence of normal and atherosclerotic tissues e) presence of all stages of atherosclerotic lesions f) identifiable zones of transition from normal to atherosclerotic regions for indexing lesion initiation, development, and growth
  • 3. Model Attributes (continued) g) identifiable major lesion types for indexing lesion changes and the effects of treatment (retardation, stabilization, regression) h) defined sites for replicate measures, whose locations are referenced to the carotid bifurcation or an external marker i) gross dimensions, histology, and chemical composition at reference sites as defined by accepted independent measurements (e.g. X-ray, raman, infrared spectroscopies) j) multiple reference sites indexed for easy use k) stability for extended time periods (e.g. years) in chemical composition, and gross and microscopic morphology
  • 4. The Model Holder should: a) have clear identification permanently attached b) allow easy access to indexed sites c) provide mounting that makes the model resistant to normal wear d) be compatible with conventional and state-of-the-art MRI instrumentation e) be amenable to convenient handling and shipping f) have attached instructions for handling by the user
  • 5. Model Documentation should provide: a) accurate description of the gross and histological composition, with location of defined, indexed referenced sites for rapid orientation in scanning and measurement b) an atlas of acceptable images c) specifications for MR image acquisition at indexed reference sites d) defined quality control parameters with the accepted “in control” range e) periodic verification of stability of MRI characteristics performed by the reference laboratory f) life history of the model
  • 6. CCA BIF ICA ECA Cadaveric human carotid artery used for Reference Standard Tissue was perfusion fixed at mean arterial pressure before excision. Most of the periadventitial tissue has been removed. The common carotid artery (CCA), bifurcation (BIF), external carotid artery (ECA), and internal carotid artery (ICA) are clearly seen.
  • 7. Cadaveric Carotid Specimens ( models of the native carotid arteries) -contain intima, media, adventitia, and some perivascular soft tissue -enable study of range of lesion types -allow comparison of left/right carotids -give reproducible images over >1 year -provide stable reference for: -intra-laboratory standardization -inter-laboratory standardization for multicenter clinical trials
  • 8. Holder for imaging cadaveric carotid tissues. The tubes (3.0cm diameter) are secured in four holes drilled in a plastic insert (8.0cm diameter) fabricated from two 100ml plastic beakers fastened top-to-top by superglue. The insert is placed in a larger plastic bottle (12.0cm diameter, 22.0cm tall) fitted with a water inlet at the bottom and outlet at the top for temperature control. A pair of phased array coils (6cm Ultra Image/Pathway) is fastened around the outside of the larger outer bottle with velcro strips. Tissues are imaged in a GE Horizon 1.5T clinical MRI system with 10cm FOV, 2 NEX, 3mm slice thickness. TR/TE for the different contrast sequences: 3000/34 (PDW), 650/14 (T1W), and 3000/108 (T2W).
  • 9. 1.5T MR images of left and right human cadaveric carotid arteries above, at, and below their bifurcation. The plaque within each tissue slice (red area) has been identified by a semi-automatic algorithm. 3D reconstructions from multiple slices are used to calculate the aggregate volumes.
  • 10. PDW T1W T2W MR images of human cadaveric carotids acquired on a GE Horizon LX 1.5T system fitted with UltraImage/Pathway phased array coils (also used for human carotid studies in vivo). The slices (3mm) begin at the bifurcation (left) and descend to the common carotid artery (right). Proton density-, T1-, and T2- weighted images show plaque heterogeneity