2. Technological advances now
allow single-cell transcriptomics
Lane K et at. (2017) Measuring Signaling and RNA-
Seq in the Same Cell Links Gene Expression to
Dynamic Patterns of NF-κB Activation. Cell Syst
3. Challenge: Brain tissues consists
of intricate neural layers & circuits
Brain Tissue
Single Neurons
4. Research Questions
• How does the process of cellular dissociation alter
gene expression?
• Does the process of cellular dissociation alter our
ability to identify biologically meaningful patterns of
gene expression?
15. Thanks!
• Hans Hofmann & all the
Hofmann lab members
• UT Genomic Sequencing &
Analysis Facility
• UT Center for Computational
Biology & Bioinformatics
• Misha Matz & Rachel Wright
• Boris Zemelman, Laura Colgin
• Andre Fenton, Maddy Kao,
Ain Chung, Juan Marcos
Alarcon at NYU & SUNY
Downstate
• The Neural Systems &
Behavior Community
• Software/Data Carpentry
• NSF, NIH, Grass Foundation,
Promega, JAX Lab
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