Presentation of Capabilities and Competitive Advantage of Neuromics' umbilical derived human stem cells and MSCGro high performance growth and differentiation media.

1. Potent Human Mesenchymal Stem Cells and Media
Capabilities and Competitive Analysis
www.neuromics.com

2. STEM CELL PRODUCTS
Focus on Mesenchymal Stem Cells-Why MSCs?
• Numerous Clinical Uses:
Joint disease & injury, autoimmunity, stroke, organ failure (heart,
liver, kidney & lung), MS, promotion of HSC engraftment,
regenerative medicine, cancer, hearing loss, etc.
• Readily Available:
Blood, adipose (fat) tissue, bone marrow, teeth, etc

3. GFP-Expressing Human MSCs Catalog Number SC00A4
Image: FITC of Single Human MSC GFP-Expressing Human MSCs
express label in the cytoplasm.
Illustrates morphological detail of human mesenchymal stem cells.

4. GFP-Expressing Human MSCs
FITC Image FITC + PC showing selection

5. Red Fluorescent Human Mesenchymal Stem Cells
Self-Renewal Transfer of label to
chondrocyte (25 mM
PGE2)

6. MSC Growth in Low Serum MSCGro™ Compared to Lonza MSC-GM
MSCGroTM at Day 3 MSCGroTM at Day 4
Lonza at Day 3 Lonza at Day 4

7. Human MSC Growth in MSCGroTM vs Competitors Media:
MSCGroTM results in faster growth & 2-3-fold greater cell recovery

8. 160
Real Time Stability of MSCGro
140 Compared to Competitor's Medium
120
Doubling Time (Hrs)
100 MSCGro at 5% O2
MSCM-ScienCell at 5% O2
80
MSCGro at 20% O2
60 (Adipose MSCs by third party)
40
20
0
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14
o
Months at 2-8 C

9. Humanized Complete & Humanized Powder MSCGro™

10. Differentiation of Human MSCs Cultured in MSC-GroTM Medium
Chondrocyte Stained Adipocytes Stained with Osteoblast Stained
with Alcian Blue Oil Red with Alizarin Red

11. Competitive Advantages of MSCGroTM
• MSC growth rate/cell recovery > Lonza, InVitrogen, Stem Cell
Technologies, ScienCell & ZenBio media.
• MSC quality/potency (Lumenesc™) > Lonza, InVitrogen and Stem Cell
Technologies media.
• Vastly improved real time stability.
• Medium function unaffected by freezing.
• Out-performs competitors at both reduced & ambient O2
• Supports self-renewal of human, rat & mouse MSCs
• Also supports primary cultures: fibroblasts (human & rat); rat
cardiomyocytes & possibly hepatocytes
• Provided complete & ready to use as is.