Enzymes in clinical use and importance of enzymes in diagnosis, enzymes which are increased in plasma during certain disease are used to diagnose that particular defect

1. Enzymes in clinical use

2. DIAGNOSTIC VALUE OF
SERUM ENZYMES
tPA…is very important tissue
plasminogen activator…..streptokinase
activates it to destroy blood clots

3. CARDIAC BIOMARKERS
CK-MB AST LDH

4. ENZYME PROFILE IN LIVER DISEASE
ALT ALP γGT

5. ALT
 Alanine aminotransaminase
 ↑ in acute liver damage
ALP
 Alkaline phosphatase
 Bone & liver
 ↑in rickets, hyperparathyroidism, Paget disease, obstructive
jaundice, osteoblastic sarcoma.
γGT
 Gamma glutamyl transpeptidase
 ↑ in hepatobiliary obstruction, alcohol induced liver disease

6. ALP ↑ in bone diseaseS
(rickets,hyperparathyroidism, ca. Of bone,obstructive jaundice)
ACID PHOSPHATASE ↑ in prostatic carcinoma with metastasis
CREATINE KINASE(MM) ↑ in muscle disease
AMYLASE ↑ in parotitis, acute pancreatitis
• In acute pancreatitis Amylase activity is raised to
more than 1000 i.u in 24 hrs.
• Also raised in pancreatic abcess & obstruction of
pancreatic duct.