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Protein Function:
Question 1. Protein A binds to protein B with an association rate of 8.9 x 103 M-1s-1
and an overall dis...
Enzyme Kinetics
Question 1: κcat for enzyme A is 600 s-1. Experimentally, when [E] = 20nM, and
[S] = 40 µM, V0 = 9.6 µMs-1...
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Answers Test 1

  1. 1. Protein Function: Question 1. Protein A binds to protein B with an association rate of 8.9 x 103 M-1s-1 and an overall dissociation constant (Kd) of 10 nM. Calculate the dissociation rate κd. Ka = κa / κd, Kd = 1/Ka, so Kd = κd / κa 10nM = κd / 8.9 x 103M-1s-1 Κd = 8.9 x 10-5s-1 Question 2: An antibody binds to an antigen with a Kd of 5 x 10-8 M. At what concentration of antigen will θ be 0.2? Θ = [L] / [L] + Kd Θ([L] + Kd) = [L] ΘKd = [L] + Θ[L] Θ(Kd)/ (1- Θ) = [L] Solving for [L], [L] = 0.2(5 x 108M)/(1-0.2)) = 0.2(6 x 10-8M) = 1.2 x 10-8M
  2. 2. Enzyme Kinetics Question 1: κcat for enzyme A is 600 s-1. Experimentally, when [E] = 20nM, and [S] = 40 µM, V0 = 9.6 µMs-1. Calculate Km for this enzyme. Use Equation V0 = Kcat[Et][S] / Km + [S] 9.6 µMs-1 = (600s-1)(20µM)(40µM) / Km + 40µM Km + 40 µM = 480 µM2s-1 / 9.6 µMs-1 Km + 40 µM = 50 µM Km = 10 µM Question 2 we went over in class.

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