4. MICROBIOLOGICAL ASSAY OF ANTIBIOTICS
Inhibition of growth of
concentrations of the
Effect that produced by
known concentration of
a standard preparation
of the antibiotic having
a known activity.
7. Take bacterial
Take petri dish with
sample in the media
Perforation in media
antibiotics in the
Place in the
Measure zone of
9. TURBIDIMETRIC METHOD
Bacteria with nutrient medium
Take 5 different concentration of standard and
sample concentration antibiotics
Take 1ml of standard and sample concentration of
antibiotics in different test tubes
Add 9ml of bacteria with nutrient medium solution
in each tubes
11. WHAY MICROBIOLOGICAL ASSAY OF ANTIBIOTICS?
The inhibition of growth may be utilized for
demonstrating the therapeutic efficacy of antibiotics.
Any subtle change in the antibiotic molecule which
may not be detected by chemical methods will be
revealed by a change in the antimicrobial activity.
Resolving doubts regarding possible change in
potency of antibiotics and their preparations.