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Protein Chemistry Bsc Nursing & Bpt
- 13. Classification of Proteins Shape & Size Functional Properties Solubility & Physical Properties Fibrous Globular Defence proteins Contractile Proteins Hormones Enzymes Structural proteins Respiratory Proteins Simple Proteins Conjugated Proteins Derived Proteins Protamines Scleroproteins Glutelins Gliadins (Prolamines) Globulins Albumins Histones Nucleo proteins Metallo proteins Lipoproteins Phospho proteins Chromo proteins Glycoproteins Muco Proteins Primary derived Secondary derived Proteans Coagulated proteins Metaproteins Proteoses peptides Peptones
- 14. Simple Proteins Basic Protein, N.A. + Histone=Nucleoprotein, Porphyrin+Histone=conjugated Protein, Repressor of template activity of DNA in synthesis of RNA’ Hemoglobin Globin Histone His & lys rich Alkaline Arginine, Histidine _ NH3 Water, Dil. Acid, Salt solution Histones Salmine Sardine Cyprinine(of fish sperms & testes) Basic protein, N.A+protamine=Nucleoprotein 7.4 Cysteine Tryptophan tyrosine Arginine rich _ Water Dilute acid Dilute alkali Dilute NH3 small Protamine Eg. Use pI AA- AA+ Heat coagulability Insolubility Solubility Size & shape Protein type
- 15. Simple proteins contd. Rice Oryzein, Wheat Glutelin Glutamic acid rich + Water, Neutral salt solution Dilute acid, Dilute alkali Large molecules Glutelins Wheat- Gliadin, Barley Hordein Plant Protein Lysine Proline Water, Salt soln., Absolute alcohol Alcohol(50-80% C2H5OH) Gliadin (Prolamines) Heme+globulin=Hemopexin, Heme+metal=Transferrin, Ceruloplasmin, Heme+Carbohydrate=Immunoglobulin, Eggs Ovoglobulin Milk Lactoglobulin Legumes Legumin Precipitated by half saturation with ammonium sulfate or full saturation with sodium chloride. + water Dilute neutral salt solutions Globulins Legumes—legumelin, Cereals—Leucosin, Egg—Ovalbumin Milk--Lactalbumin Precipitated out of solution by saturation by ammonium sulfate. 4.7 Glycine Cagulable by heat Product insoluble in water & dil. Salt solution Water, Dilute salt solution Albumin
- 16. Simple proteins contd. scleroproteins Found in Connective tissue, In Yellow elastic fibres, In ligament, tendon Formed in large amount during pregnancy Elastin is hydrolyzed by pancreatic elastase Cysteine, Methionine, 5-Hydroxylysine, Histidine Alanine, Leucine, Valine, Proline Elastins Found in connective tissue,Bone Collagen Tannic acid tough, hard, Collagen boiling gelatin highly soluble, easily digestible cool gel no tryptophan Insoluble in all neutral salt solvents Long, thin, partially crystalline Collagen Present in chidermal tissuesuch as horn, hair, nails, wool, hoofs, feathers(supporting structures) spider’s web, silk, reptilian scales Cysteine absent Hard keratin His:Lys:Arg 1:4:12, Soft or pseudokeratins A.A. not in same ratio, Neurokeratins 1:2:2, Human Hair alpha keratin cysteine rich, Beta Keratin Glycine, Alanine rich low Fibrous Keratins