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Introduction infertility clinic in delhi,india priory clinic - copy

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Introduction infertility clinic in delhi,india priory clinic - copy

  1. 1. Priory Clinic Best IVF Expert India www.ivfexpertindia.com Contact: 9810119072 Dr. S.Basu | Dr. S.N.Basu
  2. 2. Cryopreservation Treatment • At Priory Clinic, we recognize that several diverse factors could affect a couple’s fertility; we also understand how much anxiety infertility can cause to couples. We have state of the art facilities at Priory Clinic that help you to get to the root of the problem. We strive to provide you with a solution through various methods. Cryopreservation is one of the facilities offered at our fertility clinic. Let me tell you all about it.
  3. 3. Understand what it is The word ‘Cryo’ comes from the Greek word ‘kruos’, which means icy cold; in the combining form, it refers to low temperatures. So basically it means preserving in very low temperatures. This is essentially a process whereby cells, whole tissues, or anything else that is likely to be damaged due to chemical reactions or the passage of time, are preserved, by cooling them to extremely low temperatures. In infertility clinics, embryos, gametes, sperms, and ovarian and testicular tissue are the materials preserved in this manner, for use in the future. This method is more efficient and cost effective, and more convenient for the couple. To protect the materials from further damage that may be brought about by ice formation while freezing, they are usually coated with ‘Cryoprotectants’. At our clinic it is a two-step process: First, we collect the sample from the couple in special straw like tubes. Next, they are stored at sub-zero temperatures in special solutions; before use, these specimens are thawed. There are several types of Cryopreservation, and they differ in these areas: specimen type, temperature, type of cryopreservant, freezing and thawing times.
  4. 4. The Advantages • Improves Efficiency: The extracted sperms, eggs or embryos can be used whenever needed, saving money, energy and time. The woman does not have to go through repeated ovarian stimulation; single oocyte retrieval is sufficient. • Avoids hyper stimulation syndrome: Multiple medical stimulation of the ovaries is risky and strenuous for the ovaries, and can even cause death in extreme cases. With this method, that risk is eliminated as stimulation is done only once. • R & D: Many preserved embryos are used for research; often, a couple conceives in the first attempt and they donate the rest of the embryos for research. • Eliminates need for multiple implants: Earlier, to improve the chances of conception, multiple implantations were made but nowadays in a single attempt, only one or maximum two embryos are implanted, with the others being frozen for later use. • Preservation of Fertility: Couples facing risk of becoming infertile, like those undergoing chemotherapy, or women with early menopause genes, can greatly benefit from preserving their eggs, sperms and embryos for future use.
  5. 5. The Process Cryopreservation is basically a process of freezing human tissues to keep them ‘live’ for a long period of time. This means that the cells have to be preserved undamaged, so that once thawed, they can function normally, and become active. There are several different conventions to be followed for achieving this. Cryosurvival refers to the process of thawing the frozen tissues and making them active again. The necessity of maintaining water movement inside the cell membrane to ensure the cell’s survival makes this a very challenging task. In spite of the low external temperature, if water movement inside the cell is constant, it will maintain the same pressure. Do bear in mind that all human cells do not have the same structure; the water movement varies across cells. Hence, it is crucial to determine the protocol for freezing and thawing, depending on the material being preserved.
  6. 6. The Process Let us take a look at the methods of cryopreservation of different cells in our clinic. 1. Gametes: preserving the male gametes is quite simple, as thousands of sperms are found even in a little semen. Even with less than perfect preservation techniques, sufficient number of sperms will remain viable. Survival is nearly double when the semen is taken via the normozoospermic method as compared to the oligoozoospermic method. Preparation of the semen is also crucial. The survival rate can be increased by a further 12% - 27% with the ‘swim up’ technique and 23% with the centrifugal technique. 2. Oocytes: Preservation of these cells is far more difficult as eggs are extremely delicate and their outer shells get injured easily even in spite of the precautions taken. However, emergent technologies in this field are now helping us to preserve oocytes much better than before; in fact, the survival rate is up to 60%. Yet, the incidence of live births with the use of survived oocytes is still low in comparison to fresh oocytes. One of the innovations has been the use of rapid freezing; it was observed that slow freezing diminished the survival chances of the oocyte.
  7. 7. The Process 3. Embryos: Embryos can be cryopreserved efficiently and successfully. Research has shown that while the chance for a live birth through a preserved embryo is around 5%, it could be doubled by preserving blastocytes. In my opinion it is prudent to fertilize the oocyte in lab, and then freezing it for preservation. Whether or not the implanted embryo develops into a baby depends on, a) the embryo’s developmental stage at freezing, and b), the implantation time post thawing. At Priory Clinic we recommend the freezing of the embryo when it has grown to a size of over 6 cells. Implantation of such embryos has a higher chance at success. Waiting for the thawed embryo to commence division and replication before implanting it, also increases its survival odds. 4. Testicular and Ovarian Tissue: There may be cases where there is paucity of time for ovarian stimulation or gamete collection; either the patient may not have reached puberty, or has been prescribed treatment that may cause infertility (like, say, chemotherapy for cancer). Gonadal tissue is collected to preserve fertility. However, readers need to understand that this is still in the experimental stage, and wide success has not yet been reported.
  8. 8. Priory IVF Clinic- Contact Us Address: 652, Ambika Apartments, Sector 14, Rohini , Delhi-110085 New Delhi-110085,India Contact: +91 9810119072 Email Id: priory.clinic@hotmail.com Website: www.ivfexpertindia.com