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Polymerase Chain Reaction     By Whitney Housley
Definition-• The polymerase chain reaction aka PCR is a  method used to analyze a short sequence of  DNA or RNA, it is use...
Steps
Advantages• Much faster than using vectors• Only a little bit of target DNA is needed
Disadvantages• Are to synthesize primers, we need to know  the sequence flanking the DNA segment of  interest• Only applie...
What is it used for?•   Medical and biological research•   Cloning•   Diagnosis of hereditary diseases•   Identification o...
Cited Sources• Mrs. Scherman’s Honors Biology Class Notes• <http://www.open-access-  biology.com/pcr/pcr-boulter.html>.• <...
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Polymerase chain reaction powerpoint

  1. 1. Polymerase Chain Reaction By Whitney Housley
  2. 2. Definition-• The polymerase chain reaction aka PCR is a method used to analyze a short sequence of DNA or RNA, it is used to reproduce selected sections or DNA or RNA
  3. 3. Steps
  4. 4. Advantages• Much faster than using vectors• Only a little bit of target DNA is needed
  5. 5. Disadvantages• Are to synthesize primers, we need to know the sequence flanking the DNA segment of interest• Only applies to short DNA fragments, mostly less than 5 kb
  6. 6. What is it used for?• Medical and biological research• Cloning• Diagnosis of hereditary diseases• Identification of fingerprints• Forensic sciences• Paternity testing• Diagnosis of infectious diseases
  7. 7. Cited Sources• Mrs. Scherman’s Honors Biology Class Notes• <http://www.open-access- biology.com/pcr/pcr-boulter.html>.• <http://www.medicinenet.com/pcr_polymera se_chain_reaction/article.htm>.

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