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Faizan Ahmed Sheikh, Raminder Preet Pal Singh, Jang Bahadur Singh, Parveen Lehana/
International Journal of Engineering Research and Applications
(IJERA) ISSN: 2248-9622 www.ijera.com
Vol. 3, Issue 4, Jul-Aug 2013, pp.242-247
242 | P a g e
Effect Of Microwaves On The Resistance Of Aloe Vera Leaves
Faizan Ahmed Sheikh1
, Raminder Preet Pal Singh1
, Jang Bahadur Singh2
,
Parveen Lehana2
1
Deptt. of Electrical and Electronics, ARNI University, Kathgarh, Himachal Pradesh, India
2
D.S.P Lab, Deptt. of Physics and Electronics, University of Jammu, Jammu, J&K, India
ABSTRACT
For centuries plants has been exposed to
natural electromagnetic sources such as radiation
from sun, space, and earth. Today, in addition to
this natural radiation and owing to immediate
technological progresses these plants are further
exposed to man-made electromagnetic radiation
(EM). A variety of products and applications in
our day to day life makes use of a number of
forms of electromagnetic energy. One such form
of energy is Microwave. Microwaves are non-
ionizing electromagnetic radiations. These are
electromagnetic waves with wavelengths ranging
from as long as one meter to as short as one
millimetre, or equivalently, with frequencies
between 300 MHz (0.3 GHz) and 300 GHz. These
frequencies may affect the quality of micro waved
treated Aloe Vera leaves. The effect of microwave
on Aloe Vera leaves was studied. Aloe Vera plant
has been chosen in view of the fact that it has
numerous applications in dermatology. It is also
famous as “Lily of Desert”. The leaves of Aloe
Vera contain a soothing thick sap that is valuable
for healing and curing of wounds and diseases.
The objective of this study is to investigate the
changes in electrical properties of Aloe Vera
plants after exposure to different amount of
microwaves. It was found that the on exposure to
microwaves the Aloe Vera Leaves first showed a
marked increase in the current across it with a
slight increase in the voltage but afterwards it
adjusted itself to the normal value.
Keywords - Aloe Vera, D. C. resistance, Effect of
microwaves, Klystron, Microwave test bench,
Surface electrodes.
I. INTRODUCTION
Since atmosphere microwave window
allows diffusion of cosmic rays with frequencies
ranging within GHz domain, there is always a
microwave element within the environment emission
background [1]. Microwave spectrum falls in
between the Infra-Red (IR) and the Radio frequency
(RF) segment of the electromagnetic radiation.
Microwaves are electromagnetic waves with
wavelengths ranging from as long as one meter to as
short as one millimeter, or equivalently, with
frequencies between 300 MHz (0.3GHz) and
300GHz. This broad definition includes both UHF
and EHF (millimeter waves), and various sources
use different boundaries. In all cases, microwave
includes the entire SHF band (3 to 30 GHz, or 10 to
1cm) at minimum, with RF engineering often putting
the lower boundary at 1 GHz (30 cm), and the upper
around 100 GHz (3 mm). Microwaves are used in
plywood, paint, inks, synthetic rubber, and medical
related manufacturing industries [2]. Microwaves are
non ionizing radiations. These radiations are the part
of the electromagnetic spectrum. Wireless
telecommunication devices enhance the exposure of
radio and microwave frequencies in the
environment. The energy content of the
electromagnetic (EM) waves is related to the
frequency as:
E hv where E = Electric field, h = Plank’s
constant, and ν = frequency.
The plants have cellular receptors which are use
to observe changes in their surroundings. These
changes are of different types and differ from plant
to plant. All living organisms generate and conduct
electrochemical impulses all the way through their
different tissues and organs. It has been
demonstrated experimentally the properties of
temperature sensing in plants [3]. The plants also
show reaction to the exciting electrical signals.
These signals may set off different physiological
reactions [4]. The main physical signal in any
organism is electrical signal. The electrical signals
are capable of transmitting signals at a long distance.
Recently, biologists have revealed that the
significance of physiological activities depends upon
the electrical signals [5]. Plants play a significant
role in the living world as main producers of food
and oxygen, therefore it would be advantageous to
investigate their relations with today’s increased
exposure to radio and microwave frequency fields.
The effect of microwave causes alteration in
electrical properties of Aloe Vera plants. The new
and energetic area of scientific research is the studies
associated to the microwave effects. Microwave
effects can be generally classified into thermal and
non thermal. It is well known that microwave fields
have poor biological effects at high power levels.
But, effects at low power levels have not been fully
implicit. Radiations are known to encourage
physiological and genetic modifications [6-9]. The
Faizan Ahmed Sheikh, Raminder Preet Pal Singh, Jang Bahadur Singh, Parveen Lehana/
International Journal of Engineering Research and Applications
(IJERA) ISSN: 2248-9622 www.ijera.com
Vol. 3, Issue 4, Jul-Aug 2013, pp.242-247
243 | P a g e
objective of this paper is to investigate the changes
in electrical properties of the Aloe Vera leaves
exposed to microwave radiations. The details of
Aloe Vera plant is presented in Section II. The
methodology is given in Section III and results &
discussions in Section IV along with the conclusions
in Section V.
II. ALOE VERA
Aloe Vera is a stem less plant having a
height of 60–90 cm (24–36 inches) tall, spreading
by offsets. The stem has dormant root buds that
develop to form new roots. The leaves are thick and
fleshy, green to grey-green, with some varieties
showing white flecks on the upper and lower stem
surfaces [10]. Aloe Vera plant gel is a lively
ingredient in a number of different skin care
products that facilitates to treat a wide variety of
different problems of skin. Healing properties of
Aloe Vera plant make it so beneficial for skin care.
It helps in restoring skin's natural beauty this is one
of the most important benefits of Aloe Vera. The
plant can survive in hot temperatures ranging from
40 degrees Celsius down to freezing temperatures as
far as the root of the plant is not damaged [11]. Its
thick leaves include the water supply for the plant to
survive long periods of drought. These leaves have a
high capability of retaining the water. Transparent
gel from the pulp of the fleshy leaves of Aloe Vera
has been used topically for thousands of years to
treat wounds, skin infections, burns, and numerous
other dermatologic conditions. Dried latex from the
inner lining of the leaf has traditionally been used as
an oral laxative [12]. Aloe Vera gel is used as an
ingredient in commercially available lotions, yogurt,
beverages, and some desserts [13-14]. The use of
Aloe Vera gel in cosmetics has a function analogous
to anti-aging effects of vitamin A derivatives [15].
A. Water Storing Mechanism of Aloe Vera
The Aloe Vera store water in their enlarged
fleshy leaves stems or roots as shown below. This
allows them to survive in arid environments. The
Aloe Vera leaf has a clear, inner, fleshy, portion. It is
rich in nutrients and very healthy for both internal
and external consumption. Fig. 1. shows Split Aloe
Vera leaf with fleshy clear inner part.
B. Physiology of Aloe Vera
Aloe Vera is a perennial liliaceous plant
which has juicy green leaves attached at the stem in
a whorled pattern. It has thick fibrous root which
produces large basal leaves. The leaves are one to
two feet long, and three to four centimeters wide,
gradually tapering to a point. They are whitish green
on both sides and bear spiny teeth on the margins.
Fig. 2. shows the whorled pattern of Aloe Vera
leaves.
Fig. 1. Split Aloe Vera leaf with fleshy clear inner
part.
Fig. 2. Whorled pattern of Aloe Vera leaves.
C. Anatomy of Aloe Vera
It consists of three main parts:
1. Aloe Vera Rind: The protective, green,
outer covering of the leaf is called Aloe
Vera Rind.
2. Aloe Vera Latex: This is the middle part of
the Aloe Vera leaf which is bitter. It is in
between the inner fleshy part and outer part
like a sap. This sap is contained in special
tubules which form a portion of the nutrient
tubes of the vascular bundles located just
beneath the wax-covered, thick green rind
of the leaf.
3. Aloe Vera Gel: This is the inner portion of
the leaf and is very fleshy. It is rich in
nutrients and is recommended for
consumption. The gel, which consist the
bulk of the leaf substance, serves as the
water storage space organ for the plant.
This gel, which may be separated as a semi-
solid “fillet” before processing, contains
more than 200 different substances. Chief
among these are polysaccharides, glycol
proteins, vitamins, mineral and enzymes.
Faizan Ahmed Sheikh, Raminder Preet Pal Singh, Jang Bahadur Singh, Parveen Lehana/
International Journal of Engineering Research and Applications
(IJERA) ISSN: 2248-9622 www.ijera.com
Vol. 3, Issue 4, Jul-Aug 2013, pp.242-247
244 | P a g e
Fig. 3. Anatomy of Aloe Vera leaf.
D. Economical Importance of Aloe Vera
It is highly appreciated because of its use in
pharmaceutical industries, folk medicine, healthcare,
cosmetic and food industries. It also has high
economic value among all the plant species [16]. In
homeopathic medicine Aloe Vera is used for
haemorrhoids. In India Aloe Vera has been referred
to as "Kumari’ in Ayurveda treatments. A number of
selected Aloe Vera species are valued for their
beauty and ornamental value.
E. Electrical Properties of Aloe Vera
1.Electrical Resistance: Resistance is the
opposition offered by the Aloe Vera Plant
when the current is passed through the Aloe
Vera tissue .The phenomenon of polarization
occurs, when the current is passed through it,
i.e. at the level of each electrode a double
layer of ions get deposited which acts as an
insulator prevents the current from passing.
2.Electrical Capacitance: Capacitance is the
ability of a body to store an electrical charge.
When the current is passed through the Aloe
Vera tissue it gets electrically charged and the
charge is stored in the form of energy. It acts
like a parallel plate capacitor.
If the charges on the plates are +q and −q,
and V give the voltage between the plates, then the
capacitance C will be:
q
C
V

That gives the voltage/current relationship:
( )
( )
dV t
I t C
dt

Thus, for a fixed value of capacitance, the rate of
change of voltage is directly proportional to current.
III. METHODOLOGY
The investigations were carried out on the
leaves of the Aloe Vera plant to study the effect of
microwaves on the electrical resistance of the leaves.
The plant was grown in a pot containing fertile soil
under natural environmental conditions. In this
research, microwave setup was installed and these
microwaves were applied on the Aloe Vera leaf to
record any change in the resistance of the leaf. The
experimental setup can be understood below in
Fig. 4.
Fig. 4. Microwave setup for exposure of Aloe Vera
leaf to microwaves.
First of all the Aloe Vera leaf was taken
and the two surface electrodes were attached on the
leaf at a distance of 6.5 cm. With these two
electrodes the electrical connections were
established as shown below in the Fig. 5, where e1
and e2 are surface electrodes on the Aloe Vera leaf.
DC
+ -
10 k
Potentiometer
µ A
e1
e2
Fig. 5. Circuit diagram for calculating resistance of
the Aloe Vera leaf.
The voltage as well as current across the
leaf was measured without exposing it to
microwaves. Then the microwave setup was
established that consisted of Klystron along with the
Klystron power supply. The voltage across the
Klystron power supply when switched on was 300
V. Klystron is a specialized liner-beam vacuum tube
which is used as an amplifier for high frequencies.
Here the klystron is used as a waveguide for
coupling microwave energy into and out of the
device. Now, the two surface electrodes were
attached to the Aloe Vera Leaf at a distance of 6.5
cm and further this Aloe Vera leaf bearing electrodes
was kept at a distance of 41 cm away from the
Klystron in between the two horns having a gap of
Faizan Ahmed Sheikh, Raminder Preet Pal Singh, Jang Bahadur Singh, Parveen Lehana/
International Journal of Engineering Research and Applications
(IJERA) ISSN: 2248-9622 www.ijera.com
Vol. 3, Issue 4, Jul-Aug 2013, pp.242-247
245 | P a g e
10 cm from each other. The distance between the
Aloe Vera leaf was 5 cm from either horn. The
length & width of the horn was 15 cm & 10 cm
respectively. After that the apparatus consisting of
Aloe Vera leaf bearing electrodes and the horns was
wrapped with the thin aluminum foil and was
exposed to microwaves for every 2400 seconds.
IV. RESULTS AND DISCUSSIONS
Investigations were carried out using
microwaves for the determination of the electrical
properties of the Aloe Vera plant. An Aloe Vera
tissue acts as an ionic conductor and when the direct
current is passed through an ionized solution the
well-known phenomenon of polarization occurs, i.e.
very rapidly at the level of each electrode a double
layer of ions gets deposited which acts as an
insulator and prevents the current from passing. Aloe
Vera leaf was given microwave exposure for same
durations of 2400 s, 4800 s, 7200 s, 14400 s and this
experiment was repeated 4 times on the same Aloe
Vera leaf. The results are explained in Table I.
Table I. Resistance calculated every 2400 s of
microwave exposure.
Microwave
Exposed
Time (s)
Voltage
(V)
Current (µA) Resistance
(MΩ)
0 4.78 6 0.79
2400 4.78 9 0.53
4800 4.81 6 0.8
7200 4.78 5 0.95
14400 4.79 5.5 0.87
The Resistance (in mega ohms) curve of
this circuit is represented in Fig. 6 below with
respect to the Time, where VCR variations are
variation in voltage, current and resistance in volts,
microampere and mega ohms respectively. For the
first 2400 s of the exposure to microwaves the
current across the Aloe Vera leaf was increased to 9
µA from 6µA which was without exposure to
microwaves. The voltage across the leaf remained
constant i.e. 4.78 V and the resistance calculated was
0.53 mega-ohms. After that the leaf was again
exposed to microwaves for next 4800 s, 7200 s and
14400 s and it was found that the current first
adjusted itself to 6 µA, 5 µA and 5.5 µA respectively
and the voltages recorded were 4.81 V, 4.78 V, and
4.79 V respectively.
Fig. 6. Resistance curve of Aloe Vera leaf with
respect to the exposed time.
The calculated values are represented in
form of Histogram.
Fig. 7. Variation of voltage, current and resistance
of Aloe Vera leaf with respect to the exposed time.
V. CONCLUSION
The investigations were carried out to study
the variation of resistance in the Aloe Vera leaves.
The experiments have shown that the resistance of
the Aloe Vera leaves showed a specific non-linear
function of time of microwave exposure. Also it
shows that whenever the dc power supply is given to
the Aloe Vera leaf, before microwave exposure it
offers a high resistance to the applied voltage and
after the microwave exposure the resistance
decreases slightly and then readjusts itself to the
normal value. Moreover this idea of electrical
processes in plants may lead to elaboration of new
approaches and mean of objective diagnostics of
physiological state of the plants.
ACKNOWLEDGEMENTS
The authors would like to express a deep
sense of gratitude and thanks to Mr. Adil Bhat,
M.Phil. Chemistry, for extending his knowledge and
help. The authors would also like to thanks to their
parents who have been the ultimate source of
Faizan Ahmed Sheikh, Raminder Preet Pal Singh, Jang Bahadur Singh, Parveen Lehana/
International Journal of Engineering Research and Applications
(IJERA) ISSN: 2248-9622 www.ijera.com
Vol. 3, Issue 4, Jul-Aug 2013, pp.242-247
246 | P a g e
support. Their constant guidelines and
encouragement made this possible.
REFERENCES
[1] M. Ursache, G. Mindru1, D.E. C Reangea,
F.M. Tufescu, C. Goiceanu“Al. I. Cuza”,
The Effects of High Frequency
Electromagnetic Waves on The vegetal
Organisims (University, Faculty of Physics,
11A Blvd. Carol I, Iaşi, Romania).
[2] By Lalaram Guyadin April 20, 2009
Environ Mentors Program Mentor: Dr. John
J. Ohab Cardozo High School.
[3] C. Plieth, Temperature Sensing by Plants
Calcium-Permeable Channels as Primary
Sensors, A Model, The Journal of
Membrane Biology, 172, 1999, 121–127.
[4] S. Lautner, T. Grams, R. Matyssek, and J.
Fromm, Characteristics of Electrical
Signals in Poplar and Responses in
Photosynthesis, Plant Physiology, 138,
2005, 2200-2209.
[5] K.Trebacz, H.Dziubinska, E. Krol,
Electrical signals in long distance
communication in plants. In: Baluska F,
Mancuso S, Volkmann D, in plants –
neuronal aspects of plant life. Berlin,
Heidelberg: Springer-Verlag, 2006, 277–
290.
[6] M. Tafforeau, M. C. Verdus, R. Charlionet,
A. Cabin- Flaman and C. Ripoll, Two-
Dimensional Electrophoresis Investigation
of Short Term Response of Flax Seedlings
to Cold Shock, Electrophoresis, 23,15,2002,
2534-2540.
[7] M. Tafforeau, M. C. Verdus, V. Norris, G.
White, M. Demarty, M. Thellier and C.
Ripoll, SIMS Study of the Calcium-
Deprivation Step Related to Epidermal
Meris-tem Production Induced in Flax by
Cold Shock or Radia-tion from a GSM
Telephone, Journal of Trace and Mi-
croprobe Techniques, 20, 4, 2002, 611-623.
[8] M. Tafforeau, M. C. Verdus, V. Norris, G.
J. White, M. Cole, M. Demarty, M. Thellier
and C. Ripoll, Plant Sen-sitivity to Low
Intensity 105 GHz Electromagnetic Ra-
diation, Bioelectromagnetics, 25, 6, 2004,
403-407.
[9] M. Tafforeau, M. C. Verdus, V. Norris, C.
Ripoll and M. Thellier, Memory Processes
in the Response of Plants to Environmental
Signals, Plant Sign Behav.,1, 2006, 9-14
[10] Available online: Yates A. (2002) Yates
Garden Guide. Harper Collins Australia.
[11] M.A. Saeed, I. Ahmad, U.Yaqub, Akbar,
M.A. Waheed, A. Saleem, and Nasir-ud-Di.
Aloe Vera a plant of vital significance,
Science vision, 9, 1-2.
[12] Available online: Aloe Vera (Aloe Vera),
MayoClinic.com. 2012-09-01. Retrieved
2012-11-09
[13] Available online: Reynolds, T. (2004) Aloe
Vera’s: The Genus Aloe Vera. CRC Press.
[14] Available online: Liza Armstrong. Clean
and green. Australian Broadcasting
Corporation. Archived from the original
on 2008-05-24. Retrieved 2008-06-20.
[15] I. Danho, Potential reversal of
chronological and photo-aging of the skin
by topical application of natural
substances. Physiotherapy Research,
7,1993, 53-56.
[16] T. Reynolds, A.C. Dweck, J.
Ethnopharmacol, Aloe Vera leaf gel: a
review update, 68, 1999, 3.
BIOGRAPHY
Er. Faizan Ahmed Sheikh received
his B.E. Degree in Electronics and
Communication Engineering from
Model Institute of Engineering and
Technology, Kot Bhalwal, Jammu
(J&K) affiliated to University of
Jammu, Jammu (J&K). He is presently doing
M.Tech. in Electrical and Electronics Engineering
from ARNI University, Kathgarh (H.P). His fields of
interests are Microcontrollers, Microwaves and
Image processing. He also did four weeks industrial
training from Bharat Sanchar Nigam Limited,
Exchange Road, Jammu.
Mr. Raminder Preet Pal Singh
received his M.Tech. Degree in
Electronics and Communication
Engineering from Beant College of
Engineering and Technology,
Gurdaspur (Punjab) affiliated to
Punjab Technical University, Jalandhar (Punjab). He
is presently working as Associate Dean and Head in
ECE/EEE Department, ARNI University, Kathgarh
(Himachal Pradesh) and pursuing Ph.D in Nano-
technology. His research interests include Low-
Power VLSI Design, Digital System Design, and
Microcontroller Based System Design. He has
published research papers in reputed International
Journals/Conferences.
Mr. Jang Bahadur Singh is pursuing
Ph.D in biomedical speech signal
processing in the Department of
Physics and Electronics, University of
Jammu under the guidance of Dr.
Parveen Lehana. His research interests include
Faizan Ahmed Sheikh, Raminder Preet Pal Singh, Jang Bahadur Singh, Parveen Lehana/
International Journal of Engineering Research and Applications
(IJERA) ISSN: 2248-9622 www.ijera.com
Vol. 3, Issue 4, Jul-Aug 2013, pp.242-247
247 | P a g e
speech, biomedical signal processing, robotics, and
microwaves. He has more than 10 publications.
Dr. Parveen Lehana received his
Master’s degree in Electronics from
Kurukshetra University in 1992 and
qualified UGC-CSIR-NET-JRF in
Physical Sciences and Electronics.
He joined University of Jammu in
1995 as Assistant Professor. Presently, he is working
as Associate Professor in Physics and Electronics
Department, University of Jammu. He did his
research from I.I.T Bombay in the area of signal
processing. He has good experience of guiding
M.Tech, M.Phil. and Ph.D students. He has more
than 120 research papers to his credit. His fields of
interests are speech signals processing, computer
vision, nanotechnology, and microwaves.

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  • 1. Faizan Ahmed Sheikh, Raminder Preet Pal Singh, Jang Bahadur Singh, Parveen Lehana/ International Journal of Engineering Research and Applications (IJERA) ISSN: 2248-9622 www.ijera.com Vol. 3, Issue 4, Jul-Aug 2013, pp.242-247 242 | P a g e Effect Of Microwaves On The Resistance Of Aloe Vera Leaves Faizan Ahmed Sheikh1 , Raminder Preet Pal Singh1 , Jang Bahadur Singh2 , Parveen Lehana2 1 Deptt. of Electrical and Electronics, ARNI University, Kathgarh, Himachal Pradesh, India 2 D.S.P Lab, Deptt. of Physics and Electronics, University of Jammu, Jammu, J&K, India ABSTRACT For centuries plants has been exposed to natural electromagnetic sources such as radiation from sun, space, and earth. Today, in addition to this natural radiation and owing to immediate technological progresses these plants are further exposed to man-made electromagnetic radiation (EM). A variety of products and applications in our day to day life makes use of a number of forms of electromagnetic energy. One such form of energy is Microwave. Microwaves are non- ionizing electromagnetic radiations. These are electromagnetic waves with wavelengths ranging from as long as one meter to as short as one millimetre, or equivalently, with frequencies between 300 MHz (0.3 GHz) and 300 GHz. These frequencies may affect the quality of micro waved treated Aloe Vera leaves. The effect of microwave on Aloe Vera leaves was studied. Aloe Vera plant has been chosen in view of the fact that it has numerous applications in dermatology. It is also famous as “Lily of Desert”. The leaves of Aloe Vera contain a soothing thick sap that is valuable for healing and curing of wounds and diseases. The objective of this study is to investigate the changes in electrical properties of Aloe Vera plants after exposure to different amount of microwaves. It was found that the on exposure to microwaves the Aloe Vera Leaves first showed a marked increase in the current across it with a slight increase in the voltage but afterwards it adjusted itself to the normal value. Keywords - Aloe Vera, D. C. resistance, Effect of microwaves, Klystron, Microwave test bench, Surface electrodes. I. INTRODUCTION Since atmosphere microwave window allows diffusion of cosmic rays with frequencies ranging within GHz domain, there is always a microwave element within the environment emission background [1]. Microwave spectrum falls in between the Infra-Red (IR) and the Radio frequency (RF) segment of the electromagnetic radiation. Microwaves are electromagnetic waves with wavelengths ranging from as long as one meter to as short as one millimeter, or equivalently, with frequencies between 300 MHz (0.3GHz) and 300GHz. This broad definition includes both UHF and EHF (millimeter waves), and various sources use different boundaries. In all cases, microwave includes the entire SHF band (3 to 30 GHz, or 10 to 1cm) at minimum, with RF engineering often putting the lower boundary at 1 GHz (30 cm), and the upper around 100 GHz (3 mm). Microwaves are used in plywood, paint, inks, synthetic rubber, and medical related manufacturing industries [2]. Microwaves are non ionizing radiations. These radiations are the part of the electromagnetic spectrum. Wireless telecommunication devices enhance the exposure of radio and microwave frequencies in the environment. The energy content of the electromagnetic (EM) waves is related to the frequency as: E hv where E = Electric field, h = Plank’s constant, and ν = frequency. The plants have cellular receptors which are use to observe changes in their surroundings. These changes are of different types and differ from plant to plant. All living organisms generate and conduct electrochemical impulses all the way through their different tissues and organs. It has been demonstrated experimentally the properties of temperature sensing in plants [3]. The plants also show reaction to the exciting electrical signals. These signals may set off different physiological reactions [4]. The main physical signal in any organism is electrical signal. The electrical signals are capable of transmitting signals at a long distance. Recently, biologists have revealed that the significance of physiological activities depends upon the electrical signals [5]. Plants play a significant role in the living world as main producers of food and oxygen, therefore it would be advantageous to investigate their relations with today’s increased exposure to radio and microwave frequency fields. The effect of microwave causes alteration in electrical properties of Aloe Vera plants. The new and energetic area of scientific research is the studies associated to the microwave effects. Microwave effects can be generally classified into thermal and non thermal. It is well known that microwave fields have poor biological effects at high power levels. But, effects at low power levels have not been fully implicit. Radiations are known to encourage physiological and genetic modifications [6-9]. The
  • 2. Faizan Ahmed Sheikh, Raminder Preet Pal Singh, Jang Bahadur Singh, Parveen Lehana/ International Journal of Engineering Research and Applications (IJERA) ISSN: 2248-9622 www.ijera.com Vol. 3, Issue 4, Jul-Aug 2013, pp.242-247 243 | P a g e objective of this paper is to investigate the changes in electrical properties of the Aloe Vera leaves exposed to microwave radiations. The details of Aloe Vera plant is presented in Section II. The methodology is given in Section III and results & discussions in Section IV along with the conclusions in Section V. II. ALOE VERA Aloe Vera is a stem less plant having a height of 60–90 cm (24–36 inches) tall, spreading by offsets. The stem has dormant root buds that develop to form new roots. The leaves are thick and fleshy, green to grey-green, with some varieties showing white flecks on the upper and lower stem surfaces [10]. Aloe Vera plant gel is a lively ingredient in a number of different skin care products that facilitates to treat a wide variety of different problems of skin. Healing properties of Aloe Vera plant make it so beneficial for skin care. It helps in restoring skin's natural beauty this is one of the most important benefits of Aloe Vera. The plant can survive in hot temperatures ranging from 40 degrees Celsius down to freezing temperatures as far as the root of the plant is not damaged [11]. Its thick leaves include the water supply for the plant to survive long periods of drought. These leaves have a high capability of retaining the water. Transparent gel from the pulp of the fleshy leaves of Aloe Vera has been used topically for thousands of years to treat wounds, skin infections, burns, and numerous other dermatologic conditions. Dried latex from the inner lining of the leaf has traditionally been used as an oral laxative [12]. Aloe Vera gel is used as an ingredient in commercially available lotions, yogurt, beverages, and some desserts [13-14]. The use of Aloe Vera gel in cosmetics has a function analogous to anti-aging effects of vitamin A derivatives [15]. A. Water Storing Mechanism of Aloe Vera The Aloe Vera store water in their enlarged fleshy leaves stems or roots as shown below. This allows them to survive in arid environments. The Aloe Vera leaf has a clear, inner, fleshy, portion. It is rich in nutrients and very healthy for both internal and external consumption. Fig. 1. shows Split Aloe Vera leaf with fleshy clear inner part. B. Physiology of Aloe Vera Aloe Vera is a perennial liliaceous plant which has juicy green leaves attached at the stem in a whorled pattern. It has thick fibrous root which produces large basal leaves. The leaves are one to two feet long, and three to four centimeters wide, gradually tapering to a point. They are whitish green on both sides and bear spiny teeth on the margins. Fig. 2. shows the whorled pattern of Aloe Vera leaves. Fig. 1. Split Aloe Vera leaf with fleshy clear inner part. Fig. 2. Whorled pattern of Aloe Vera leaves. C. Anatomy of Aloe Vera It consists of three main parts: 1. Aloe Vera Rind: The protective, green, outer covering of the leaf is called Aloe Vera Rind. 2. Aloe Vera Latex: This is the middle part of the Aloe Vera leaf which is bitter. It is in between the inner fleshy part and outer part like a sap. This sap is contained in special tubules which form a portion of the nutrient tubes of the vascular bundles located just beneath the wax-covered, thick green rind of the leaf. 3. Aloe Vera Gel: This is the inner portion of the leaf and is very fleshy. It is rich in nutrients and is recommended for consumption. The gel, which consist the bulk of the leaf substance, serves as the water storage space organ for the plant. This gel, which may be separated as a semi- solid “fillet” before processing, contains more than 200 different substances. Chief among these are polysaccharides, glycol proteins, vitamins, mineral and enzymes.
  • 3. Faizan Ahmed Sheikh, Raminder Preet Pal Singh, Jang Bahadur Singh, Parveen Lehana/ International Journal of Engineering Research and Applications (IJERA) ISSN: 2248-9622 www.ijera.com Vol. 3, Issue 4, Jul-Aug 2013, pp.242-247 244 | P a g e Fig. 3. Anatomy of Aloe Vera leaf. D. Economical Importance of Aloe Vera It is highly appreciated because of its use in pharmaceutical industries, folk medicine, healthcare, cosmetic and food industries. It also has high economic value among all the plant species [16]. In homeopathic medicine Aloe Vera is used for haemorrhoids. In India Aloe Vera has been referred to as "Kumari’ in Ayurveda treatments. A number of selected Aloe Vera species are valued for their beauty and ornamental value. E. Electrical Properties of Aloe Vera 1.Electrical Resistance: Resistance is the opposition offered by the Aloe Vera Plant when the current is passed through the Aloe Vera tissue .The phenomenon of polarization occurs, when the current is passed through it, i.e. at the level of each electrode a double layer of ions get deposited which acts as an insulator prevents the current from passing. 2.Electrical Capacitance: Capacitance is the ability of a body to store an electrical charge. When the current is passed through the Aloe Vera tissue it gets electrically charged and the charge is stored in the form of energy. It acts like a parallel plate capacitor. If the charges on the plates are +q and −q, and V give the voltage between the plates, then the capacitance C will be: q C V  That gives the voltage/current relationship: ( ) ( ) dV t I t C dt  Thus, for a fixed value of capacitance, the rate of change of voltage is directly proportional to current. III. METHODOLOGY The investigations were carried out on the leaves of the Aloe Vera plant to study the effect of microwaves on the electrical resistance of the leaves. The plant was grown in a pot containing fertile soil under natural environmental conditions. In this research, microwave setup was installed and these microwaves were applied on the Aloe Vera leaf to record any change in the resistance of the leaf. The experimental setup can be understood below in Fig. 4. Fig. 4. Microwave setup for exposure of Aloe Vera leaf to microwaves. First of all the Aloe Vera leaf was taken and the two surface electrodes were attached on the leaf at a distance of 6.5 cm. With these two electrodes the electrical connections were established as shown below in the Fig. 5, where e1 and e2 are surface electrodes on the Aloe Vera leaf. DC + - 10 k Potentiometer µ A e1 e2 Fig. 5. Circuit diagram for calculating resistance of the Aloe Vera leaf. The voltage as well as current across the leaf was measured without exposing it to microwaves. Then the microwave setup was established that consisted of Klystron along with the Klystron power supply. The voltage across the Klystron power supply when switched on was 300 V. Klystron is a specialized liner-beam vacuum tube which is used as an amplifier for high frequencies. Here the klystron is used as a waveguide for coupling microwave energy into and out of the device. Now, the two surface electrodes were attached to the Aloe Vera Leaf at a distance of 6.5 cm and further this Aloe Vera leaf bearing electrodes was kept at a distance of 41 cm away from the Klystron in between the two horns having a gap of
  • 4. Faizan Ahmed Sheikh, Raminder Preet Pal Singh, Jang Bahadur Singh, Parveen Lehana/ International Journal of Engineering Research and Applications (IJERA) ISSN: 2248-9622 www.ijera.com Vol. 3, Issue 4, Jul-Aug 2013, pp.242-247 245 | P a g e 10 cm from each other. The distance between the Aloe Vera leaf was 5 cm from either horn. The length & width of the horn was 15 cm & 10 cm respectively. After that the apparatus consisting of Aloe Vera leaf bearing electrodes and the horns was wrapped with the thin aluminum foil and was exposed to microwaves for every 2400 seconds. IV. RESULTS AND DISCUSSIONS Investigations were carried out using microwaves for the determination of the electrical properties of the Aloe Vera plant. An Aloe Vera tissue acts as an ionic conductor and when the direct current is passed through an ionized solution the well-known phenomenon of polarization occurs, i.e. very rapidly at the level of each electrode a double layer of ions gets deposited which acts as an insulator and prevents the current from passing. Aloe Vera leaf was given microwave exposure for same durations of 2400 s, 4800 s, 7200 s, 14400 s and this experiment was repeated 4 times on the same Aloe Vera leaf. The results are explained in Table I. Table I. Resistance calculated every 2400 s of microwave exposure. Microwave Exposed Time (s) Voltage (V) Current (µA) Resistance (MΩ) 0 4.78 6 0.79 2400 4.78 9 0.53 4800 4.81 6 0.8 7200 4.78 5 0.95 14400 4.79 5.5 0.87 The Resistance (in mega ohms) curve of this circuit is represented in Fig. 6 below with respect to the Time, where VCR variations are variation in voltage, current and resistance in volts, microampere and mega ohms respectively. For the first 2400 s of the exposure to microwaves the current across the Aloe Vera leaf was increased to 9 µA from 6µA which was without exposure to microwaves. The voltage across the leaf remained constant i.e. 4.78 V and the resistance calculated was 0.53 mega-ohms. After that the leaf was again exposed to microwaves for next 4800 s, 7200 s and 14400 s and it was found that the current first adjusted itself to 6 µA, 5 µA and 5.5 µA respectively and the voltages recorded were 4.81 V, 4.78 V, and 4.79 V respectively. Fig. 6. Resistance curve of Aloe Vera leaf with respect to the exposed time. The calculated values are represented in form of Histogram. Fig. 7. Variation of voltage, current and resistance of Aloe Vera leaf with respect to the exposed time. V. CONCLUSION The investigations were carried out to study the variation of resistance in the Aloe Vera leaves. The experiments have shown that the resistance of the Aloe Vera leaves showed a specific non-linear function of time of microwave exposure. Also it shows that whenever the dc power supply is given to the Aloe Vera leaf, before microwave exposure it offers a high resistance to the applied voltage and after the microwave exposure the resistance decreases slightly and then readjusts itself to the normal value. Moreover this idea of electrical processes in plants may lead to elaboration of new approaches and mean of objective diagnostics of physiological state of the plants. ACKNOWLEDGEMENTS The authors would like to express a deep sense of gratitude and thanks to Mr. Adil Bhat, M.Phil. Chemistry, for extending his knowledge and help. The authors would also like to thanks to their parents who have been the ultimate source of
  • 5. Faizan Ahmed Sheikh, Raminder Preet Pal Singh, Jang Bahadur Singh, Parveen Lehana/ International Journal of Engineering Research and Applications (IJERA) ISSN: 2248-9622 www.ijera.com Vol. 3, Issue 4, Jul-Aug 2013, pp.242-247 246 | P a g e support. Their constant guidelines and encouragement made this possible. REFERENCES [1] M. Ursache, G. Mindru1, D.E. C Reangea, F.M. Tufescu, C. Goiceanu“Al. I. Cuza”, The Effects of High Frequency Electromagnetic Waves on The vegetal Organisims (University, Faculty of Physics, 11A Blvd. Carol I, Iaşi, Romania). [2] By Lalaram Guyadin April 20, 2009 Environ Mentors Program Mentor: Dr. John J. Ohab Cardozo High School. [3] C. Plieth, Temperature Sensing by Plants Calcium-Permeable Channels as Primary Sensors, A Model, The Journal of Membrane Biology, 172, 1999, 121–127. [4] S. Lautner, T. Grams, R. Matyssek, and J. Fromm, Characteristics of Electrical Signals in Poplar and Responses in Photosynthesis, Plant Physiology, 138, 2005, 2200-2209. [5] K.Trebacz, H.Dziubinska, E. Krol, Electrical signals in long distance communication in plants. In: Baluska F, Mancuso S, Volkmann D, in plants – neuronal aspects of plant life. Berlin, Heidelberg: Springer-Verlag, 2006, 277– 290. [6] M. Tafforeau, M. C. Verdus, R. Charlionet, A. Cabin- Flaman and C. Ripoll, Two- Dimensional Electrophoresis Investigation of Short Term Response of Flax Seedlings to Cold Shock, Electrophoresis, 23,15,2002, 2534-2540. [7] M. Tafforeau, M. C. Verdus, V. Norris, G. White, M. Demarty, M. Thellier and C. Ripoll, SIMS Study of the Calcium- Deprivation Step Related to Epidermal Meris-tem Production Induced in Flax by Cold Shock or Radia-tion from a GSM Telephone, Journal of Trace and Mi- croprobe Techniques, 20, 4, 2002, 611-623. [8] M. Tafforeau, M. C. Verdus, V. Norris, G. J. White, M. Cole, M. Demarty, M. Thellier and C. Ripoll, Plant Sen-sitivity to Low Intensity 105 GHz Electromagnetic Ra- diation, Bioelectromagnetics, 25, 6, 2004, 403-407. [9] M. Tafforeau, M. C. Verdus, V. Norris, C. Ripoll and M. Thellier, Memory Processes in the Response of Plants to Environmental Signals, Plant Sign Behav.,1, 2006, 9-14 [10] Available online: Yates A. (2002) Yates Garden Guide. Harper Collins Australia. [11] M.A. Saeed, I. Ahmad, U.Yaqub, Akbar, M.A. Waheed, A. Saleem, and Nasir-ud-Di. Aloe Vera a plant of vital significance, Science vision, 9, 1-2. [12] Available online: Aloe Vera (Aloe Vera), MayoClinic.com. 2012-09-01. Retrieved 2012-11-09 [13] Available online: Reynolds, T. (2004) Aloe Vera’s: The Genus Aloe Vera. CRC Press. [14] Available online: Liza Armstrong. Clean and green. Australian Broadcasting Corporation. Archived from the original on 2008-05-24. Retrieved 2008-06-20. [15] I. Danho, Potential reversal of chronological and photo-aging of the skin by topical application of natural substances. Physiotherapy Research, 7,1993, 53-56. [16] T. Reynolds, A.C. Dweck, J. Ethnopharmacol, Aloe Vera leaf gel: a review update, 68, 1999, 3. BIOGRAPHY Er. Faizan Ahmed Sheikh received his B.E. Degree in Electronics and Communication Engineering from Model Institute of Engineering and Technology, Kot Bhalwal, Jammu (J&K) affiliated to University of Jammu, Jammu (J&K). He is presently doing M.Tech. in Electrical and Electronics Engineering from ARNI University, Kathgarh (H.P). His fields of interests are Microcontrollers, Microwaves and Image processing. He also did four weeks industrial training from Bharat Sanchar Nigam Limited, Exchange Road, Jammu. Mr. Raminder Preet Pal Singh received his M.Tech. Degree in Electronics and Communication Engineering from Beant College of Engineering and Technology, Gurdaspur (Punjab) affiliated to Punjab Technical University, Jalandhar (Punjab). He is presently working as Associate Dean and Head in ECE/EEE Department, ARNI University, Kathgarh (Himachal Pradesh) and pursuing Ph.D in Nano- technology. His research interests include Low- Power VLSI Design, Digital System Design, and Microcontroller Based System Design. He has published research papers in reputed International Journals/Conferences. Mr. Jang Bahadur Singh is pursuing Ph.D in biomedical speech signal processing in the Department of Physics and Electronics, University of Jammu under the guidance of Dr. Parveen Lehana. His research interests include
  • 6. Faizan Ahmed Sheikh, Raminder Preet Pal Singh, Jang Bahadur Singh, Parveen Lehana/ International Journal of Engineering Research and Applications (IJERA) ISSN: 2248-9622 www.ijera.com Vol. 3, Issue 4, Jul-Aug 2013, pp.242-247 247 | P a g e speech, biomedical signal processing, robotics, and microwaves. He has more than 10 publications. Dr. Parveen Lehana received his Master’s degree in Electronics from Kurukshetra University in 1992 and qualified UGC-CSIR-NET-JRF in Physical Sciences and Electronics. He joined University of Jammu in 1995 as Assistant Professor. Presently, he is working as Associate Professor in Physics and Electronics Department, University of Jammu. He did his research from I.I.T Bombay in the area of signal processing. He has good experience of guiding M.Tech, M.Phil. and Ph.D students. He has more than 120 research papers to his credit. His fields of interests are speech signals processing, computer vision, nanotechnology, and microwaves.