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Tomato Genetics and Breeding Research at Penn State Majid R. Foolad Department of Horticulture, and The Intercollege Graduate Degree Programs in Genetics and Plant Physiology The Pennsylvania State University
Goals ,[object Object],[object Object],[object Object],[object Object],[object Object]
Traits of Interest ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Goal 1 Identify Genetic Resources ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Screening for EB Resistance in Greenhouse
Screening for LB Resistance in High Tunnel
Screening for Drought Tolerance in Greenhouse
Screening for Salt Tolerance in Greenhouse
Genetic Source of High Fruit Lycopene Content Identified
Goal 2 Discern Genetic Basis of Traits ,[object Object]
Sensitive Parent X  Tolerant Parent F 1 X Control (n = 400) Cold (n = 400) Salt (n = 400) Treatment: Proportion  selected: Family  size: 5% 20 F 3 Families Control  Cold Salt 480 480 480 Treatment: 5% X Control  Cold Salt 480 480 480 5% X Control  Cold Salt 480 480 480 Progeny: X 20 F 3 Families 20 F 3 Families F 2 Estimation of Heritability and Correlation between Traits
Goal 3 Develop Genetic Markers and Maps ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Goal 4 Identify genes or QTLs for traits of interest ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
TG185  (14.9) CT172  (5.0) CT118  (2.0) TG318  (3.9) CT202  (2.0) XLRR.370  (7.1) RLRR.220  (6.0) PK12.340  (27.9) CT93  (23.9) CT242  (11.1) TG441  (13.1) CEL5  (10.7) CT101  (0.0) Chr1 Chr5 Chr9 Chr2 TG507  (1.5) TG250  (0.9) TG620  (0.9) CT59  (36.3) TG337  (36.2) TG484  (22.1) A35.9  (0.0) A35.7  (18.9) TG582  (6.5) CT176  (5.3) CT205  (6.3) TG608  (3.6) XLRR.380  (3.8) CLRR.490  (1.4) PK12.1200  (0.9) PK12.600  (0.0) Chr10 Chr3 TG214  (13.1) TG411  (26.9) XLRR.140  (8.4) TG246  (6.0) TG515  (3.1) TG388  (1.4) CT82  (2.1) S23.140  (3.5) TG621  (4.9) TG66  (3.8) A44  (8.5) TG525  (2.3) CT22  (19.1) CT141  (21.3) TG132  (0.0) TG517  (2.2) TG114  (0.0) Chr7 TG128  (21.1) C21  (31.9) CT52  (11.2) CT135  (2.8) TG61  (10.1) PK12.300  (9.6) TG174  (4.0) TG183  (12.8) CT226  (15.2) TG216  (17.6) TG499  (0.0) Chr11 Chr4 TG163  (4.3) CEL68  (4.3) CT173  (5.4) CT73  (6.0) CT50  (13.7) TG65  (15.0) TG652  (3.8) CT157  (5.3) PK34.300  (2.6) PK34.150  (8.9) TG586  (8.0) TG483  (0.0) Chr8 CT265  (25.0) CT68  (4.1) TG294  (22.4) TG330  (20.9) TG302  (6.4) TG349  (10.4) CT92  (14.7) TG176  (23.6) CD40  (0.0) Chr12 7.0 3.7 4.4 2.0 1.8 5.4 TG393  (26.7) TG36  (14.4) CT107c  (3.7) TG546  (8.4) CT107a  (5.7) CD17  (9.4) CT55  (3.6) A41.3  (3.6) TG147  (9.2) CT182  (9.2) TG523  (7.0) TG651  (5.8) TG508  (12.0) CT168  (0.0) TG429  (29.1) TG424  (5.6) TG421  (10.0) CT74  (8.1) TG248  (9.3) S11.210  (5.6) TG390  (7.1) TG551  (17.8) TG291  (12.5) XLRR.360  (2.1) CLRR.950  (5.6) RLRR.130  (0.0) RLRR.140  (16.7) TG254  (6.1) TG9  (1.6) C11  (6.4) CT143  (0.0) 8.2 6.8 3.8 5.3 KJB4  (14.4) CT276  (8.1) TG473  (26.3) PK12.320  (10.4) TG565  (0.8) TG283  (0.7) TG180  (3.7) AN23.390  (11.1) CT211  (6.3) CT79  (3.8) TG68  (16.2) CT100  (12.3) TG111  (16.9) CT156  (0.0) TG63  (16.4) TG403  (0.7) TG241  (6.3) CEL58  (3.6) CEL79  (5.9) CT57  (4.7) KJB3  (0.0) CC33  (10.4) CD34  (1.1) TG15  (7.0) CT287a  (3.5) CT274  (0.7) TG566  (3.0) CT234  (7.7) TG230  (0.0) CT137  (15.3) TG208a  (8.2) TG559  (15.6) TG460  (14.8) TG273  (16.9) TG223  (2.2) TG208b  (4.4) TG70  (9.1) XLRR.390  (0.0) AN23.230  (0.0) AN23.210  (4.5) TG24  (4.8) CT197  (0.0) 2.9 Chr6 2.2 EBR1.1 EBR2.1 EBR3.1 EBR5.1 EBR5.2 EBR8.1 EBR8.2 EBR12.1 EBR12.2 EBR11.1 EBR11.2 EBR10.1 EBR9.2 EBR9.1 2.4 EB  Resistance QTLs TG314  (20.7) CT107b  (8.0) TG279  (8.5) C54  (7.5) TG253  (9.4) TG365  (10.2) CG43  (8.8) CT58  (6.6) TG590  (6.2) AN23.410  (4.7) TG232  (5.0) CT83  (13.6) CT285  (0.0)
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Goal 5 Develop Tomato Breeding Lines and Cultivars
Goal 5 Develop Tomato Breeding Lines and Cultivars   ,[object Object],[object Object],[object Object]
Tomato Crossing Blocks in the GH
Seedling Preparation in the GH
Plants Growing in the Field
Goal 5 Develop Tomato Breeding Lines and Cultivars   ,[object Object],[object Object],[object Object],[object Object],[object Object]
EB-Resistant Breeding Lines ,[object Object],[object Object],[object Object],[object Object],[object Object]
High-Lycopene Breeding Lines ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
LB-Resistant Breeding Lines ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Penn State EB Resistant Tomatoes
Penn State High LYC Cherry and Grape Tomatoes Grape Cherry Others
Penn State Grape Tomato
Penn State FM Plum Tomato
Penn State Fresh Market Tomato
Penn State Processing Tomato
Penn State Hi-yielding Processing Tomatoes

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Penn State Tomato Breeding Program

  • 1. Tomato Genetics and Breeding Research at Penn State Majid R. Foolad Department of Horticulture, and The Intercollege Graduate Degree Programs in Genetics and Plant Physiology The Pennsylvania State University
  • 2.
  • 3.
  • 4.
  • 5. Screening for EB Resistance in Greenhouse
  • 6. Screening for LB Resistance in High Tunnel
  • 7. Screening for Drought Tolerance in Greenhouse
  • 8. Screening for Salt Tolerance in Greenhouse
  • 9. Genetic Source of High Fruit Lycopene Content Identified
  • 10.
  • 11. Sensitive Parent X Tolerant Parent F 1 X Control (n = 400) Cold (n = 400) Salt (n = 400) Treatment: Proportion selected: Family size: 5% 20 F 3 Families Control Cold Salt 480 480 480 Treatment: 5% X Control Cold Salt 480 480 480 5% X Control Cold Salt 480 480 480 Progeny: X 20 F 3 Families 20 F 3 Families F 2 Estimation of Heritability and Correlation between Traits
  • 12.
  • 13.
  • 14. TG185 (14.9) CT172 (5.0) CT118 (2.0) TG318 (3.9) CT202 (2.0) XLRR.370 (7.1) RLRR.220 (6.0) PK12.340 (27.9) CT93 (23.9) CT242 (11.1) TG441 (13.1) CEL5 (10.7) CT101 (0.0) Chr1 Chr5 Chr9 Chr2 TG507 (1.5) TG250 (0.9) TG620 (0.9) CT59 (36.3) TG337 (36.2) TG484 (22.1) A35.9 (0.0) A35.7 (18.9) TG582 (6.5) CT176 (5.3) CT205 (6.3) TG608 (3.6) XLRR.380 (3.8) CLRR.490 (1.4) PK12.1200 (0.9) PK12.600 (0.0) Chr10 Chr3 TG214 (13.1) TG411 (26.9) XLRR.140 (8.4) TG246 (6.0) TG515 (3.1) TG388 (1.4) CT82 (2.1) S23.140 (3.5) TG621 (4.9) TG66 (3.8) A44 (8.5) TG525 (2.3) CT22 (19.1) CT141 (21.3) TG132 (0.0) TG517 (2.2) TG114 (0.0) Chr7 TG128 (21.1) C21 (31.9) CT52 (11.2) CT135 (2.8) TG61 (10.1) PK12.300 (9.6) TG174 (4.0) TG183 (12.8) CT226 (15.2) TG216 (17.6) TG499 (0.0) Chr11 Chr4 TG163 (4.3) CEL68 (4.3) CT173 (5.4) CT73 (6.0) CT50 (13.7) TG65 (15.0) TG652 (3.8) CT157 (5.3) PK34.300 (2.6) PK34.150 (8.9) TG586 (8.0) TG483 (0.0) Chr8 CT265 (25.0) CT68 (4.1) TG294 (22.4) TG330 (20.9) TG302 (6.4) TG349 (10.4) CT92 (14.7) TG176 (23.6) CD40 (0.0) Chr12 7.0 3.7 4.4 2.0 1.8 5.4 TG393 (26.7) TG36 (14.4) CT107c (3.7) TG546 (8.4) CT107a (5.7) CD17 (9.4) CT55 (3.6) A41.3 (3.6) TG147 (9.2) CT182 (9.2) TG523 (7.0) TG651 (5.8) TG508 (12.0) CT168 (0.0) TG429 (29.1) TG424 (5.6) TG421 (10.0) CT74 (8.1) TG248 (9.3) S11.210 (5.6) TG390 (7.1) TG551 (17.8) TG291 (12.5) XLRR.360 (2.1) CLRR.950 (5.6) RLRR.130 (0.0) RLRR.140 (16.7) TG254 (6.1) TG9 (1.6) C11 (6.4) CT143 (0.0) 8.2 6.8 3.8 5.3 KJB4 (14.4) CT276 (8.1) TG473 (26.3) PK12.320 (10.4) TG565 (0.8) TG283 (0.7) TG180 (3.7) AN23.390 (11.1) CT211 (6.3) CT79 (3.8) TG68 (16.2) CT100 (12.3) TG111 (16.9) CT156 (0.0) TG63 (16.4) TG403 (0.7) TG241 (6.3) CEL58 (3.6) CEL79 (5.9) CT57 (4.7) KJB3 (0.0) CC33 (10.4) CD34 (1.1) TG15 (7.0) CT287a (3.5) CT274 (0.7) TG566 (3.0) CT234 (7.7) TG230 (0.0) CT137 (15.3) TG208a (8.2) TG559 (15.6) TG460 (14.8) TG273 (16.9) TG223 (2.2) TG208b (4.4) TG70 (9.1) XLRR.390 (0.0) AN23.230 (0.0) AN23.210 (4.5) TG24 (4.8) CT197 (0.0) 2.9 Chr6 2.2 EBR1.1 EBR2.1 EBR3.1 EBR5.1 EBR5.2 EBR8.1 EBR8.2 EBR12.1 EBR12.2 EBR11.1 EBR11.2 EBR10.1 EBR9.2 EBR9.1 2.4 EB Resistance QTLs TG314 (20.7) CT107b (8.0) TG279 (8.5) C54 (7.5) TG253 (9.4) TG365 (10.2) CG43 (8.8) CT58 (6.6) TG590 (6.2) AN23.410 (4.7) TG232 (5.0) CT83 (13.6) CT285 (0.0)
  • 15.
  • 16.
  • 19. Plants Growing in the Field
  • 20.
  • 21.
  • 22.
  • 23.
  • 24. Penn State EB Resistant Tomatoes
  • 25. Penn State High LYC Cherry and Grape Tomatoes Grape Cherry Others
  • 27. Penn State FM Plum Tomato
  • 28. Penn State Fresh Market Tomato
  • 30. Penn State Hi-yielding Processing Tomatoes