In response to clastogen DNA double strand breaks, H2AX is phosphorylated within minutes at Ser 139, and is then referred as γH2AX. Contrary to γH2AX, micronuclei are a late marker of damages either to DNA and be related to aneugens nor clastogens effect. By detecting both H2AX and micronuclei on our high content screening plateform, our assay provide a efficient tool to assess the genotoxicity properties of your compounds.

1. In response to clastogen DNA double strand breaks, H2AX is phosphorylated within minutes
at Ser 139, and is then referred as γH2AX. Contrary to γH2AX, micronuclei are a late marker
of damages either to DNA and be related to aneugens nor clastogens effect. By detecting
both H2AX and micronuclei on our high content screening plateform, our assay provide a
efficient tool to assess the genotoxicity properties of your compounds.
G E N O T O X I C I T Y
Study DNA integrity
Example of Benzo[a]pyrene
Cells: - adherent cells
- primary cells or cell line
- with or without S9
Compounds: powder or in stock solution
HepG2 cells exposed 24 hours to several concentrations of Benzo[a]pyrene.
Plates : 96 or
384 wells plates
Requirements
Micronucleus and
H2AX assay
96 or 384-well plates
CTRL B[A]P 100µM
Micronucleus
Hoechst, H2AX
Micronucleus
H2AX H2AX
For more information, go to www.hcs-pharma.com
For a quotation, contact us at welcome@hcs-pharma.com
Ask for our short report on HepG2 cells exposed to our in
house genotoxicity library !
Detection of
micronuclei
Detection of
H2AX spots