1. REMOVAL OF RED ACID DYE BY SURFACTANT MODIFIED
BAGASSE
Harshini E (14MBT0060), Dr. Sangeetha subramanian, SBST, VIT university.
Scope of the Project
Methodology
Introduction
References
Conclusion/ Summary
Discussion
Textile industry is the fastest growing industry with the requirements of several
types of dyes for dyeing and printing of clothes. The effluent from these industries is
the major cause of environmental pollution, containing smaller amounts of dye are
highly detectable, causing environmental pollution and serious health hazards such as
severe headache, profuse sweating, mental confusion and similar health hazards. Dye
is a colored substance applied to fabrics to impart a permanent colour, which is not
removable by washing or drying. W.H. Perkin was the first to use dye in 1856. As per
the reports of Central Pollution Control Board (CPCB), commercially produced dyes
and its intermediates in India tends up to a million. Based upon the chemical
structure, dyes can be classified as, Natural dyes and Synthetic dyes.
a) Extraction of surfactant from plant source and microbial source.
b) Treating of bagasse with surfactants like A10, P21, R10.
c) Comparison between three different surfactants treated with bagasse
such as BA10, BR10, BP21.
EXTRACTION OF
SURFACTANT FROM P21:
The culture inoculated in
MSM along with crude oil
and culture was
centrifuged at
10,000rpm for 15 min,
the supernatant obtained
was added to 300 ml of
acetone and is
stabilized for 24hrs for
the formation of white
brown sediment that is
obtained after
centrifugation.
EXTRACTION OF
SURFACTANT FROM R10:
10g of Reetha is diluted
in 100ml of distilled
water and placed on a
magnetic stirrer for 3hrs.
Add 200ml of Methanol
after centrifuging at
10000 rpm for 15mins
and is stabilized for
24hrs for the formation
of dark brown precipitate
that is obtained after
centrifugation.
EXTRACTION OF
SURFACTANT FROM
A10:
10g of Acacia is diluted
in 100ml of distilled
water and placed on a
magnetic stirrer
for 3hrs. Add 200ml of
Ethanol after
centrifuging at 10000
rpm for 15mins and is
stabilized for 24hrs for
the formation of dark
brown precipitate that is
obtained after
centrifugation.BAGASSE TREATED
REETHA:
2g of extracted R10
along with 2g bagasse
is diluted in 100ml of
water and kept in
orbital shaker for
24hrs. The medium is
dried under hot air
oven or sun shade
after 24hrs and is
used for further use.
BAGASSE TREATED
ACACIA:
2g of extracted A10
along with 2g
bagasse is diluted in
100ml of water and
kept in orbital shaker
for 24hrs. The
medium is dried
under hot air oven or
sun shade after
24hrs and is used for
further use.
BAGASSE TREATED
PLANOCOCCUS:
2g of extracted P21
along with 2g bagasse
is diluted in 100ml of
water and kept in
orbital shaker for 24hrs.
The medium is dried
under hot air oven or
sun shade after
24hrs and is used for
further use.
EFFECT OF pH:
Effect of various pH
4,5,6,7,8 on dye
decolorization of AR dye
using B, BR10, BA10, BP21
was studied for 120 min
and was analyzed by taking
absorbance at 510nm using
UV spectrophotometer.
EFFECT OF ADSORBANCE:
Effect of various adsorbent 20, 40, 60,
80, 100 (mg dye/l dist.H2O) on dye
decolorization of AR dye using B,
BR10, BA10, BP21 at pH 7 was
studied for 15 mins and was analyzed
by taking absorbance at 510nm using
UV spectrophotometer and R2 value
of Freundlich and Langmuir isotherm
was calculated.
PERCENTAGE OF DYE REMOVAL:
Dye removal is used to calculate the removal of dye at particular time
at particular pH and concentration of the solution and is given by a
formula,
% removal= [ (Control OD – Sample OD)/ (Control OD) ] / 100
Results
Among three surfactants such as P21, A10, R10 treated with bagasse,
BA10 shows 99.4% removal at pH 7 while BR10, BP21 shows 98.1
and 98.0 respectively at pH 7. BA10 has fast degrading activity in
short period of time when compared to BR10, BP21.
The environmental problems created by the textile industries have received
increased attention for several decades because of contaminated effluents,
which mainly arise from dyeing processes. The present project shows that
if low cost adsorbents perform well in removing dye colour they will be
used widely in the industrial sector to improve profit and to minimize the
cost in efficiency in the industrial sector. These low cost biosorbents also
offer a lot of promising benefits for commercial purposes in the future.
Various researcher are working on removal of dyes using biosorption as a
green approach , because of its low cost ,no hazardous chemical formation.
EXTRACTED
SURFACTANTS OF
A10,R10,P21.
SURFACTANTS TREATED
WITH BAGASSE
pH 30
min
60
min
90
min
120
min
4 91.09 92.5 92.5 95.9
5 94.5 94.5 95.5 96
6 95.5 95.8 95.9 96.2
7 97.1 97.2 98.5 98.9
Dye
removal
of BA10
for
different
pH
values.
pH 30
min
60
min
90
min
120
min
4 75.6 75.8 82.5 89.9
5 78.5 79.5 85.5 90
6 86.3 86.8 90.9 92.2
7 90.1 92.2 95.5 98.9
Dye
removal
of BP21
for
different
pH values.
0
20
40
60
80
100
120
0 1 2 3 4 5 6
30 mins
60 mins
90 mins
120 mins
pH 30
min
60
min
90
min
120
min
4 74.2 74.9 75.3 77.9
5 76.3 77.5 78.2 78.8
6 78.9 78.9 79 79.2
7 79 79.3 79.5 79.9
Dye
removal
of BR10
for
different
pH values.
0
10
20
30
40
50
60
70
80
90
0 1 2 3 4 5 6
30 mins
60 mins
90 mins
120 mins
ppm c q 1/c 1/q log c log q
20 13.02 0.069 0.076 14.4 1.11 -1.16
40 30.96 0.09 0.032 11.1 1.49 -1.04
60 48.12 0.118 0.02 8.47 1.68 -0.92
80 64.32 0.156 0.015 6.41 1.8 -0.8
100 80.7 0.193 0.012 5.18 1.9 -0.71
Langmuir and freundlich
isotherm of BA10 for different
concentrations at 5 mins.
Gopi, V., Akhilesh, U. and Soundararajan, N. 2012. Bioremediation potential of
individual and consortium non-adapted fungal strains on Azo dye containing
textile effluent. Adv. Appl. Sci. Res. 3(1): 303-311.
ppm c q 1/c 1/q log c log q
20 17.62 0.023 0.056 43.4 1.24 -1.63
40 35.68 0.043 0.028 23.2 1.55 -1.36
60 53.46 0.079 0.018 12.6 1.72 -1.1
80 72.48 0.196 0.013 5.1 1.86 -0.7
100 92.01 0.3 0.01 3.33 1.96 -0.522
Langmuir and freundlich
isotherm of BR10 for different
concentrations at 10mins.
Langmuir and freundlich
isotherm of BP21 for different
concentrations at 15mins.
ppm c q 1/c 1/q log c log q
20 15.7 0.043 0.063 23.25 1.19 -1.36
40 34.6 0.054 0.028 18.51 1.53 -1.26
60 52.9 0.071 0.018 14.08 1.72 -1.14
80 70.9 0.091 0.014 10.98 1.85 -1.04
100 90.1 0.099 0.011 10.1 1.95 -1