1. --IMMOBILIZED-n
FERMASE CALB : 10000
TECHNICAL DATA SHEET
FBL's Biotech Division -Inspired by life.
FermaseCALB™10000 is an immobilized enzyme catalyst of Candida antarctica lipase B expressed in yeast cells.
Product type
Fermase CALB™ 10000 is based on covalent immobilization on polyacrylate beads.
Application
Resolution of chiral alcohols and amines; synthesis oHatty-acid esters; versatile applications in organic synthesis;
exhibit good to excellent stereoselectivity.
Description
Creamish white to white coloured regularly shaped beads with defined particle size distribution. Colour may vary
from batch to batch and the colour intensity is not an indication ofthe product quality.
Particle size
300-500 IJm > 95.0 %
Enzyme activity
NLT 2500 TBU units per g dry weight (Tributyrin hydrolysis)
NLT 10000 PLU units per g dry weight (Propyllaurate synthesis)
Dry weight
NLT60%
Unit definition
One TBU enzyme unit corresponds to amount of enzyme catalyzing the formation of 1 micromole of butyric acid at
40·C, pH7.5 at 10% (vlv) Tributyrin concentration perminute permlenzyme in the milieu of 100 mMsodium phosphate
buffer.
One PLU enzyme unit corresponds to synthesis of 1 micromole propyllaurate perminute per g ofimmobilized enzyme
at60·Cfrom lauric acidand l-propanol.
Reaction schematics
For TBU activity
(C4 H70 )-O-CH 2
I
I
(C4 H70 )-O-CH 2
(C4H70)-O-CH2
H2C - OH
+ 3H20
I
Fermase CALB™ 10000
+ HC-OH
I
H2C-OH
Butyric acid
H
H
Glycerol
H
I I I
I I I
H H H
HO-C-C-C-H
Lauric acid
CH2-COOH
~
Tributyrin
For PLU activity
3CH3-CH2 -
l-Propanol
FermaseCALBTM10000~/ ' V 0 ~ + H20
II
o
Propyllaurate

2. --IMMOBILIZED-n
FERMASE CALB : 10000
Stability profile of Fermase CALB™1 0000
12000
10000
Activity
(PLUlgdry
beads)
Activity
8000
(PLU/g dry
beads)
6000
4000
2000
O+----.----r-.----.----.-.----.----r-,-----,
<;)~ ~o' o~'l',·- ~,}. ~~e .:J>~e ,p'<.e ~e~e ~o<:l' ~o~
~~~ o-~~ »# ~e ~e~ _10.",0 "a: l-0 10.0,0
,,>
<""
'"' <"" <Q~'
'-~
O+---.-----.----r-r-...----.-----.---.-r-.-----.
o
1
234
5
6
7
8
9
16000
14000
12000
10000
8000
6000
4000
2000
10 11
pH
Storage stability (5°C) Fermase CALB™ 10000
25000
20000
Activity
15000
(PLU/gdry
beads)
10000
5000
O+---.-----r----,,.-.-----.-----.----r-.---....----.-----r----,
o
20 40 60 80 100120140 160 180 200220240
Days
Advantages
•
•
Storage & product stability
Cool and dry conditions are recommended for storage. The recommended temperature for storing is
below 1DoC. When stored under these conditions, the product will maintain its declared activity for at
least 12 months.
Safety
Fermase CALB™ 10000 is very safe to handle, as it does not generate any aerosols. However, avoid
raising dust. Avoid contact with skin and eyes. Take up the spilled dust by mechanical means. Avoid
forming aerosols, wash contaminated clothing and ensure sufficient ventilation.
Material support data
•
•
Packing size
Minimum pack size
Bulkpacksize
Higheractivity
Broad application
•
Good stability
Technical data sheet
Material safety data sheet
•
•
Certificate ofanalysis
Protocol for assay methods
1kg
25kg
/(/iI. Fermenta Biotech Limited
Manufactured By:
'DIL' Complex, Ghodbunder Road, Majiwada, Thane(West)-40061 0, Maharashtra, India.
Tel: +91 2267980800/888 • Fax: +91 22 6798 0899
Email: info@fermentabiotech.com